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Protein

Malate--CoA ligase subunit beta

Gene

mtkA

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + malate + CoA = ADP + phosphate + malyl-CoA.

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Pathwayi: formaldehyde assimilation via serine pathway

This protein is involved in the pathway formaldehyde assimilation via serine pathway, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway formaldehyde assimilation via serine pathway and in One-carbon metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi197 – 1971Magnesium or manganeseUniRule annotation
Metal bindingi199 – 1991Magnesium or manganeseUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 10874ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-4228.
MEXT272630:GBY6-1654-MONOMER.
UniPathwayiUPA00927.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate--CoA ligase subunit beta (EC:6.2.1.9)
Alternative name(s):
MTK-beta
Malate thiokinase
Malyl-CoA synthetase
Gene namesi
Name:mtkA
Ordered Locus Names:MexAM1_META1p1730
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Malate--CoA ligase subunit betaPRO_0000102888Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1730.

Structurei

3D structure databases

ProteinModelPortaliP53594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 244236ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CMV. Bacteria.
COG0045. LUCA.
HOGENOMiHOG000007059.
KOiK14067.
OMAiERDCTQV.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVHEYQAKE LLASFGVAVP KGAVAFSPDQ AVYAATELGG SFWAVKAQIH
60 70 80 90 100
AGARGKAGGI KLCRTYNEVR DAARDLLGKR LVTLQTGPEG KPVQRVYVET
110 120 130 140 150
ADPFERELYL GYVLDRKAER VRVIASQRGG MDIEEIAAKE PEALIQVVVE
160 170 180 190 200
PAVGLQQFQA REIAFQLGLN IKQVSAAVKT IMNAYRAFRD CDGTMLEINP
210 220 230 240 250
LVVTKDDRVL ALDAKMSFDD NALFRRRNIA DMHDPSQGDP REAQAAEHNL
260 270 280 290 300
SYIGLEGEIG CIVNGAGLAM ATMDMIKHAG GEPANFLDVG GGASPDRVAT
310 320 330 340 350
AFRLVLSDRN VKAILVNIFA GINRCDWVAE GVVKAAREVK IDVPLIVRLA
360 370 380 390
GTNVDEGKKI LAESGLDLIT ADTLTEAARK AVEACHGAKH
Length:390
Mass (Da):42,074
Last modified:September 22, 2009 - v2
Checksum:iF25CB69F899760C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601I → L in AAA62654 (PubMed:7961516).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33465 Genomic DNA. Translation: AAA62654.1.
CP001510 Genomic DNA. Translation: ACS39574.1.
PIRiB55230.
RefSeqiWP_003597633.1. NC_012808.1.

Genome annotation databases

EnsemblBacteriaiACS39574; ACS39574; MexAM1_META1p1730.
KEGGimea:Mex_1p1730.
PATRICi22509290. VBIMetExt101010_1720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L33465 Genomic DNA. Translation: AAA62654.1.
CP001510 Genomic DNA. Translation: ACS39574.1.
PIRiB55230.
RefSeqiWP_003597633.1. NC_012808.1.

3D structure databases

ProteinModelPortaliP53594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p1730.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACS39574; ACS39574; MexAM1_META1p1730.
KEGGimea:Mex_1p1730.
PATRICi22509290. VBIMetExt101010_1720.

Phylogenomic databases

eggNOGiENOG4105CMV. Bacteria.
COG0045. LUCA.
HOGENOMiHOG000007059.
KOiK14067.
OMAiERDCTQV.

Enzyme and pathway databases

UniPathwayiUPA00927.
BioCyciMetaCyc:MONOMER-4228.
MEXT272630:GBY6-1654-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTKA_METEA
AccessioniPrimary (citable) accession number: P53594
Secondary accession number(s): C5B110
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 22, 2009
Last modified: September 7, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.