P53592 (SUCC_COXBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinyl-CoA ligase [ADP-forming] subunit beta
EC=6.2.1.5

Alternative name(s):
Succinyl-CoA synthetase subunit beta
Short name=SCS-beta

Gene names

Name:	sucC
Ordered Locus Names:	CBU_1397

Organism

Coxiella burnetii (strain RSA 493 / Nine Mile phase I) [Reference proteome] [HAMAP]

Taxonomic identifier

227377 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Coxiellaceae › Coxiella › Coxiella burnetii

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + succinate + CoA = ADP + phosphate + succinyl-CoA. HAMAP-Rule MF_00558
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1. HAMAP-Rule MF_00558
Subunit structure	Heterotetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase beta subunit family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP succinate-CoA ligase (ADP-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Succinyl-CoA ligase [ADP-forming] subunit beta HAMAP-Rule MF_00558		PRO_0000102830
Regions
	Domain	9 – 245	237	ATP-grasp
	Nucleotide binding	35 – 108	74	ATP By similarity
Sites
	Metal binding	198	1	Magnesium or manganese By similarity
	Metal binding	200	1	Magnesium or manganese By similarity
Experimental info
	Sequence conflict	10	1	H → Y in CAA54876. Ref.2
	Sequence conflict	13	1	K → N in CAA54876. Ref.2
	Sequence conflict	18	1	P → A in CAA54876. Ref.2
	Sequence conflict	140 – 143	4	Missing Ref.1
	Sequence conflict	293	1	S → T in AAA61787. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53592 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 1D582B0D573F7CBC	FASTA	390	42,333
	10 20 30 40 50 60 MNLHEYQSKH LLKKYNIPVP ASEVVFNPDA AVDAAAKIGG DRWVVKAQVH AGGRGKAGGV 70 80 90 100 110 120 RLVKNKEELK SAVKALLGTR LVTYQTDERG QPVNQILVEQ TSDIARELYL GAVIDRASQR 130 140 150 160 170 180 IVFMASTEGG VEIEKVAEKS PEKILKVTID PAIGLQPFQC RQLFFGLGLQ DLKQMRSFTD 190 200 210 220 230 240 IVMGLYRLFT ERDLSLLEIN PLVITGSGEL ICLDAKINID DSALYRQSEL REMRDTTQED 250 260 270 280 290 300 EHETMAQQWE LNYIKLDGNI GCMVNGAGLA MATMDLIKLS GGDPANFLDV GGSATKERVT 310 320 330 340 350 360 EAFKIIVSDK NVKGILVNIF GGIVRCDLIA DGIISAVKEV GIDVPVVVRL EGNNAQLGAK 370 380 390 KLADSGMNII AAKGFADAAE QIVKQVGVIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Schimmels J.A., Mallavia L.P. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile phase I.
[2]	Thiele D., Willems H., Oswald W., Krauss H. Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nine Mile phase I.
[3]	"Complete genome sequence of the Q-fever pathogen, Coxiella burnetii." Seshadri R., Paulsen I.T., Eisen J.A., Read T.D., Nelson K.E., Nelson W.C., Ward N.L., Tettelin H., Davidsen T.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Brinkac L.M., Madupu R., Dodson R.J., Khouri H.M., Lee K.H., Carty H.A. , Scanlan D., Heinzen R.A., Thompson H.A., Samuel J.E., Fraser C.M., Heidelberg J.F. Proc. Natl. Acad. Sci. U.S.A. 100:5455-5460(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RSA 493 / Nine Mile phase I.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U07789 Unassigned DNA. Translation: AAA61787.1. X77919 Genomic DNA. Translation: CAA54876.1. AE016828 Genomic DNA. Translation: AAO90896.1.
RefSeq	NP_820382.1. NC_002971.3.
3D structure databases
ProteinModelPortal	P53592.
SMR	P53592. Positions 1-386.
ModBase	Search...
Protein-protein interaction databases
STRING	227377.CBU_1397.
Proteomic databases
PRIDE	P53592.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO90896; AAO90896; CBU_1397.
GeneID	1209303.
KEGG	cbu:CBU_1397.
PATRIC	17931551. VBICoxBur82552_1399.
Phylogenomic databases
eggNOG	COG0045.
HOGENOM	HOG000007059.
KO	K01903.
OMA	AREMKMD.
ProtClustDB	PRK00696.
Enzyme and pathway databases
BioCyc	CBUR227377:GJ7S-1385-MONOMER.
UniPathway	UPA00223; UER00999.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.261. 1 hit.
HAMAP	MF_00558. Succ_CoA_beta.
InterPro	IPR011761. ATP-grasp. IPR013650. ATP-grasp_succ-CoA_synth-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005811. CoA_ligase. IPR017866. Succ-CoA_synthase_bsu_CS. IPR005809. Succ_CoA_synthase_bsu. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
PANTHER	PTHR11815. PTHR11815. 1 hit.
Pfam	PF08442. ATP-grasp_2. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001554. SucCS_beta. 1 hit.
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01016. sucCoAbeta. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUCC_COXBU

Accession

Primary (citable) accession number: P53592

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 9, 2003
Last modified:	May 1, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program