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Protein

Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial

Gene

sucg-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (suca-1), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (sucl-1), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (sucl-2), Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (sucg-1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39GTPUniRule annotation1
Sitei61Important for substrate specificityUniRule annotation1
Binding sitei130GTP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Sitei131Important for substrate specificityUniRule annotation1
Metal bindingi227MagnesiumUniRule annotation1
Metal bindingi241MagnesiumUniRule annotation1
Binding sitei292Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi72 – 74GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialUniRule annotationBy similarity (EC:6.2.1.4UniRule annotation)
Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit betaUniRule annotation
Short name:
G-SCSUniRule annotation
Short name:
GTPSCSUniRule annotation
Succinyl-CoA synthetase beta-G chainUniRule annotation
Short name:
SCS-betaGUniRule annotation
Gene namesi
Name:sucg-1Imported
ORF Names:C50F7.4Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC50F7.4; CE04242; WBGene00016844; sucg-1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19MitochondrionUniRule annotationAdd BLAST19
ChainiPRO_000003335920 – 415Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialUniRule annotationAdd BLAST396

Proteomic databases

EPDiP53589.
PaxDbiP53589.
PeptideAtlasiP53589.
PRIDEiP53589.

Expressioni

Gene expression databases

BgeeiWBGene00016844.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.UniRule annotation

Protein-protein interaction databases

STRINGi6239.C50F7.4.

Structurei

3D structure databases

ProteinModelPortaliP53589.
SMRiP53589.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 258ATP-graspUniRule annotationAdd BLAST231

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni349 – 351Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
GeneTreeiENSGT00390000010170.
HOGENOMiHOG000007059.
InParanoidiP53589.
KOiK01900.
OMAiVQIEINP.
OrthoDBiEOG091G07T9.
PhylomeDBiP53589.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
MF_03221. Succ_CoA_betaG_euk. 1 hit.
InterProiView protein in InterPro
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR034722. Succ_CoA_betaG_euk.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiView protein in Pfam
PF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiView protein in PROSITE
PS01217. SUCCINYL_COA_LIG_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRAAGNLSK SMMKSQRRFL NLQEFQSKEI LEKHGCSVQN FVVASNRKEA
60 70 80 90 100
EEKWMSFGDH EYVVKAQILA GGRGKGKFIN GTKGIGGVFI TKEKDAALEA
110 120 130 140 150
IDEMIGKRLV TKQTTSEGVR VDKVMIAEGV DIKRETYLAV LMDRESNGPV
160 170 180 190 200
VVASPDGGMD IEAVAEKTPE RIFKTPIDIQ MGMTEGQSLK IAKDLQFEGK
210 220 230 240 250
LIGVAAQEIK RLYDLFIAVD ATQVEINPLV ETADGRVFCV DAKMNFDDSA
260 270 280 290 300
AYRQKEIFAY ETFEEHDPRE VDAHQFNLNY IGMDGNIACL VNGAGLAMAT
310 320 330 340 350
MDLIKLHGGE PANFLDVGGA VTEDAVFNAV RIITSDPRVK CVLINIFGGI
360 370 380 390 400
VNCATIANGV VSAVNKIGLN VPMVVRLEGT NVDAAKQIMK KSGLKILTAN
410
NLDEAAAKAV SSLPK
Length:415
Mass (Da):45,092
Last modified:October 1, 1996 - v1
Checksum:iA6B234E2A951E6E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080878 Genomic DNA. Translation: CCD67452.1.
PIRiT29296.
RefSeqiNP_501266.1. NM_068865.4.
UniGeneiCel.13261.

Genome annotation databases

EnsemblMetazoaiC50F7.4; C50F7.4; WBGene00016844.
GeneIDi177555.
KEGGicel:CELE_C50F7.4.
UCSCiC50F7.4. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiSUCB2_CAEEL
AccessioniPrimary (citable) accession number: P53589
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 25, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families