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Protein

Methionine aminopeptidase A

Gene

slr0918

Organism
Synechocystis sp. (strain PCC 6803 / Kazusa)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.UniRule annotation

Catalytic activityi

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.UniRule annotation

Cofactori

Co2+UniRule annotation, Zn2+UniRule annotation, Mn2+UniRule annotation, Fe2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe2+-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80SubstrateUniRule annotation1
Metal bindingi98Divalent metal cation 1UniRule annotation1
Metal bindingi109Divalent metal cation 1UniRule annotation1
Metal bindingi109Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi172Divalent metal cation 2; catalytic; via tele nitrogenUniRule annotation1
Binding sitei179SubstrateUniRule annotation1
Metal bindingi205Divalent metal cation 2; catalyticUniRule annotation1
Metal bindingi236Divalent metal cation 1UniRule annotation1
Metal bindingi236Divalent metal cation 2; catalyticUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMetal-binding

Protein family/group databases

MEROPSiM24.001

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine aminopeptidase AUniRule annotation (EC:3.4.11.18UniRule annotation)
Short name:
MAP AUniRule annotation
Short name:
MetAP AUniRule annotation
Alternative name(s):
Peptidase MUniRule annotation
Gene namesi
Ordered Locus Names:slr0918
OrganismiSynechocystis sp. (strain PCC 6803 / Kazusa)
Taxonomic identifieri1111708 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesMerismopediaceaeSynechocystis
Proteomesi
  • UP000001425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001489631 – 253Methionine aminopeptidase AAdd BLAST253

Proteomic databases

PaxDbiP53579

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi1148.SYNGTS_2503

Structurei

3D structure databases

ProteinModelPortaliP53579
SMRiP53579
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000030426
InParanoidiP53579
KOiK01265
OMAiSYFHGPP
PhylomeDBiP53579

Family and domain databases

CDDicd01086 MetAP1, 1 hit
HAMAPiMF_01974 MetAP_1, 1 hit
InterProiView protein in InterPro
IPR036005 Creatinase/aminopeptidase-like
IPR000994 Pept_M24
IPR001714 Pept_M24_MAP
IPR002467 Pept_M24A_MAP1
PfamiView protein in Pfam
PF00557 Peptidase_M24, 1 hit
PRINTSiPR00599 MAPEPTIDASE
SUPFAMiSSF55920 SSF55920, 1 hit
TIGRFAMsiTIGR00500 met_pdase_I, 1 hit
PROSITEiView protein in PROSITE
PS00680 MAP_1, 1 hit

Sequencei

Sequence statusi: Complete.

P53579-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDTITLLSR REIEKMRQAG QLAAALLDHL APMVQPGITT LELNDEAEKW
60 70 80 90 100
TKAHGAISAP LGYNGFPKSI CTSINEVICH GIPHRKRVLQ AGDIINVDVT
110 120 130 140 150
PIVDGYHGDC SRTFFVGTPS PVAEKLVKVT EECLRLGIEA VKPGGKIGDI
160 170 180 190 200
GAAIQSHAEA QGFSVVRDFV GHGISKIFHT APQIPHYGKA GKGKRLRPGM
210 220 230 240 250
VFTIEPMINE GTWEAVLLDD GWTAITKDGK LSAQFEHTIA VTEDGVEILT

LGE
Length:253
Mass (Da):27,235
Last modified:October 1, 1996 - v1
Checksum:iBABEA87651501D72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000022 Genomic DNA Translation: BAA10466.1
PIRiS75731

Genome annotation databases

EnsemblBacteriaiBAA10466; BAA10466; BAA10466
KEGGisyn:slr0918

Similar proteinsi

Entry informationi

Entry nameiMAP11_SYNY3
AccessioniPrimary (citable) accession number: P53579
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health