P53569 (CEBPZ_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCAAT/enhancer-binding protein zeta

Alternative name(s):
CCAAT-box-binding transcription factor
Short name=CBF
Short name=CCAAT-binding factor

Gene names

Name:	Cebpz
Synonyms:	Cbf2, Cebpa-rs1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1052 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stimulates transcription from the HSP70 promoter.
Subcellular location	Nucleus.
Tissue specificity	Ubiquitous.
Sequence similarities	Belongs to the CBF/MAK21 family.
Sequence caution	The sequence AAB01503.1 differs from that shown. Reason: Frameshift at position 998. The sequence BAE34799.1 differs from that shown. Reason: Frameshift at position 1036.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-templated Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CBF1 (identifier: P53569-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CBF2 (identifier: P53569-2)

The sequence of this isoform differs from the canonical sequence as follows:
     455-461: SELANKL → KRCWIQA
     462-1052: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1052	1052	CCAAT/enhancer-binding protein zeta		PRO_0000173471
Amino acid modifications
	Modified residue	629	1	Phosphoserine By similarity
	Modified residue	641	1	Phosphoserine Ref.3
	Modified residue	837	1	Phosphoserine By similarity
	Modified residue	972	1	Phosphoserine By similarity
	Modified residue	977	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	455 – 461	7	SELANKL → KRCWIQA in isoform CBF2.		VSP_000853
	Alternative sequence	462 – 1052	591	Missing in isoform CBF2.		VSP_000854
Experimental info
	Sequence conflict	112	1	A → T in BAE22857. Ref.2
	Sequence conflict	112	1	A → T in BAE34655. Ref.2
	Sequence conflict	146	1	A → P in BAE22857. Ref.2
	Sequence conflict	146	1	A → P in BAE34655. Ref.2
	Sequence conflict	196	1	P → L in BAE22857. Ref.2
	Sequence conflict	196	1	P → L in BAE34655. Ref.2
	Sequence conflict	318	1	M → L in BAE22857. Ref.2
	Sequence conflict	318	1	M → L in BAE34655. Ref.2
	Sequence conflict	422	1	G → A in BAE22857. Ref.2
	Sequence conflict	422	1	G → A in BAE34655. Ref.2
	Sequence conflict	471	1	T → S in AAB01503. Ref.1
	Sequence conflict	652	1	G → E in BAE22857. Ref.2
	Sequence conflict	652	1	G → E in BAE34655. Ref.2
	Sequence conflict	875	1	T → A in BAE22857. Ref.2
	Sequence conflict	875	1	T → A in BAE34655. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform CBF1 [UniParc]. Last modified April 18, 2006. Version 2. Checksum: 8518FBAA30D9C41C	FASTA	1,052	120,262
	10 20 30 40 50 60 MSADQEPVAF LAKQPWRPKQ VTEDPDEEDE EDGDEGKNGF SLEEVLRLGG TKQDYLMLAT 70 80 90 100 110 120 LDENEEVVDG GKKGTIDDLQ QGELESFIQN LNLAKYSKSL IEEDEPEKKE NASKKEAKLL 130 140 150 160 170 180 KVENKKQKAT EGKKTSEKKV KNKTVAEQRP ESCPVSKAKK DKQPDVFEFL ERQTMLLRPG 190 200 210 220 230 240 GKWYDMEYSG EYSLEPQPPD VVSKYKALAQ KLYEHEVSLF KSKTNNQKGG SSTWMKAIVS 250 260 270 280 290 300 SGTLADRMAA MILLIQDDAV HTLQFVETLM SLVKKKGSKQ QCLMALDTFK ELLITDLLPD 310 320 330 340 350 360 SRKLRVFSQH PFHKLEEMSS GNKDSRDRRL ILWYYEHQLK HLVAEFVQVL ETLSHDSLVT 370 380 390 400 410 420 TKTRALVAAH ELLCDKPEEE KALLVQVINK LGDPQNRIAT KASHLLEVLL RKHPNMKGVV 430 440 450 460 470 480 CGEIERLLFR SNISPKAQYY AICFLNQMVL SHEESELANK LITLYFCFFR TCIKKKDIES 490 500 510 520 530 540 KMLSAILTGV NRAYPYSQIG DDKVREQVDT LFKVLHVVNF NTSVQALMLL FQVMNSQQTI 550 560 570 580 590 600 SDRYYTALYR KMLDPGLTTC SKQAMFLNLI YKSLKADIML RRVKAFVKRL LQVTCTQMPP 610 620 630 640 650 660 FICGALYLVS EILKAKPDLR SQLDDHPESD EENFVDVGDD SDDEKFTDAD KGTATDAVKE 670 680 690 700 710 720 VESKETEPES SAEAEKPKAA SWVHFDNLKG GKQIKTYDPF SRNPLFCGAE NTTLWELKKL 730 740 750 760 770 780 SEHFHPSVAL FAKTILEGNC IQYSGDPLQD FTLMRFLDRF VYRNPKLHKG KENTDSVVMQ 790 800 810 820 830 840 PKRKHFMKNV RDLAVNSKEF LAKEESQIPV DEVFFYRYYK KVAVVKDKQK RSADEESIED 850 860 870 880 890 900 IDDEEFENMI DTFEDDNCFP PGKDDIDFAS NMKKTKGAKA DLEDSESSDG ELGDLDDDEV 910 920 930 940 950 960 SLGSMNDEDF EIDEDGGTFM DVSDDESEDA PEFADANPKA NTKKSKRKSE DDFDFAGSFQ 970 980 990 1000 1010 1020 GQKKKKKSFN DSSLFVSAEE FGHLLDENMG SKFDTIGMNA MANRDNASFK QLKWEAERDD 1030 1040 1050 WLHNRDVKSI IKKKKNFRKK MKAPQKPKRQ RK
	Isoform CBF2 [UniParc]. Checksum: 635D6ACD0D8780DB	FASTA	461	52,609
	10 20 30 40 50 60 MSADQEPVAF LAKQPWRPKQ VTEDPDEEDE EDGDEGKNGF SLEEVLRLGG TKQDYLMLAT 70 80 90 100 110 120 LDENEEVVDG GKKGTIDDLQ QGELESFIQN LNLAKYSKSL IEEDEPEKKE NASKKEAKLL 130 140 150 160 170 180 KVENKKQKAT EGKKTSEKKV KNKTVAEQRP ESCPVSKAKK DKQPDVFEFL ERQTMLLRPG 190 200 210 220 230 240 GKWYDMEYSG EYSLEPQPPD VVSKYKALAQ KLYEHEVSLF KSKTNNQKGG SSTWMKAIVS 250 260 270 280 290 300 SGTLADRMAA MILLIQDDAV HTLQFVETLM SLVKKKGSKQ QCLMALDTFK ELLITDLLPD 310 320 330 340 350 360 SRKLRVFSQH PFHKLEEMSS GNKDSRDRRL ILWYYEHQLK HLVAEFVQVL ETLSHDSLVT 370 380 390 400 410 420 TKTRALVAAH ELLCDKPEEE KALLVQVINK LGDPQNRIAT KASHLLEVLL RKHPNMKGVV 430 440 450 460 CGEIERLLFR SNISPKAQYY AICFLNQMVL SHEEKRCWIQ A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of mouse CCAAT binding factor." Hoeppner M.A., Gilbert D.J., Copeland N.G., Jenkins N.A., Linzer D.I.H., Wu B. Nucleic Acids Res. 24:1091-1098(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS CBF1 AND CBF2). Strain: BALB/c.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CBF1). Strain: C57BL/6J and CD-1. Tissue: Egg, Rathke gland and Visual cortex.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-641, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19891 mRNA. Translation: AAB01503.1. Frameshift. U19892 mRNA. Translation: AAB01504.1. AK136173 mRNA. Translation: BAE22857.1. AK158771 mRNA. Translation: BAE34655.1. AK159081 mRNA. Translation: BAE34799.1. Frameshift.
PIR	JC6067.
RefSeq	NP_001019977.1. NM_001024806.2.
UniGene	Mm.24169.
3D structure databases
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	198668. 1 interaction.
IntAct	P53569. 2 interactions.
MINT	MINT-4090575.
PTM databases
PhosphoSite	P53569.
Proteomic databases
PaxDb	P53569.
PRIDE	P53569.
Protocols and materials databases
DNASU	12607.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000024885; ENSMUSP00000024885; ENSMUSG00000024081.
GeneID	12607.
KEGG	mmu:12607.
UCSC	uc008dpk.1. mouse.
Organism-specific databases
CTD	10153.
MGI	MGI:109386. Cebpz.
Phylogenomic databases
eggNOG	COG5593.
GeneTree	ENSGT00390000006395.
HOGENOM	HOG000168357.
HOVERGEN	HBG050881.
InParanoid	P53569.
KO	K14832.
OrthoDB	EOG7NW68M.
TreeFam	TF105010.
Gene expression databases
ArrayExpress	P53569.
Bgee	P53569.
CleanEx	MM_CEBPZ.
Genevestigator	P53569.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. IPR005612. CCAAT-binding_factor. [Graphical view]
Pfam	PF03914. CBF. 1 hit. [Graphical view]
SUPFAM	SSF48371. SSF48371. 1 hit.
ProtoNet	Search...
Other
NextBio	281754.
PRO	P53569.
SOURCE	Search...

Entry information

Entry name

CEBPZ_MOUSE

Accession

Primary (citable) accession number: P53569
Secondary accession number(s): Q3TXW5, Q3TYA8, Q3UWQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	April 18, 2006
Last modified:	February 19, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program