P53566 (CEBPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCAAT/enhancer-binding protein alpha
Short name=C/EBP alpha

Gene names

Name:

Cebpa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.
Subunit structure	Interacts with UBN1 By similarity. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with PRDM16. Interacts with ZNF638; this interaction increases transcriptional activation. Ref.3 Ref.4
Subcellular location	Nucleus.
Sequence similarities	Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Activator
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acute-phase response Inferred from electronic annotation. Source: Compara brown fat cell differentiation Inferred from direct assay PubMed 18492766. Source: MGI cell maturation Inferred from mutant phenotype PubMed 16467360. Source: MGI cellular response to lithium ion Inferred from direct assay PubMed 15673614. Source: MGI cellular response to organic cyclic compound Inferred from direct assay PubMed 12055200. Source: MGI cholesterol metabolic process Inferred from mutant phenotype PubMed 18974039. Source: MGI cytokine-mediated signaling pathway Non-traceable author statement PubMed 15664994. Source: UniProtKB embryonic placenta development Inferred from genetic interaction PubMed 15509779. Source: MGI inner ear development Inferred from direct assay PubMed 21795542. Source: MGI liver development Inferred from mutant phenotype PubMed 17021047. Source: MGI lung development Inferred from mutant phenotype PubMed 16467360. Source: MGI macrophage differentiation Inferred from mutant phenotype PubMed 15073037. Source: MGI mitochondrion organization Inferred from mutant phenotype PubMed 11940593. Source: MGI negative regulation of cell proliferation Inferred from mutant phenotype PubMed 15073037. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 10859308. Source: MGI organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of fat cell differentiation Inferred from direct assay PubMed 10937998. Source: MGI positive regulation of osteoblast differentiation Inferred from direct assay PubMed 15175325. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 18632661. Source: MGI positive regulation of transcription from RNA polymerase III promoter Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to vitamin B2 Inferred from electronic annotation. Source: Compara urea cycle Inferred from direct assay PubMed 17213233. Source: MGI white fat cell differentiation Inferred from mutant phenotype PubMed 15130516. Source: MGI
Cellular_component	Rb-E2F complex Inferred from electronic annotation. Source: Compara nuclear matrix Inferred from electronic annotation. Source: Compara transcription factor complex Inferred from direct assay PubMed 12861022. Source: MGI
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 10859308. Source: MGI protein homodimerization activity Inferred from direct assay PubMed 12821655. Source: MGI sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor binding Inferred from sequence or structural similarity. Source: UniProtKB transcription regulatory region DNA binding Inferred from direct assay PubMed 18632661. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	CCAAT/enhancer-binding protein alpha		PRO_0000076614
Regions
	Domain	283 – 346	64	bZIP
	Region	287 – 314	28	Basic motif By similarity
	Region	318 – 346	29	Leucine-zipper By similarity
	Compositional bias	99 – 102	4	Poly-Gly
	Compositional bias	181 – 190	10	Poly-Pro
	Compositional bias	262 – 271	10	Poly-Gly
Amino acid modifications
	Modified residue	159	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	30 – 54	25	AFGFP…EPLGG → RLWLSPGRGPRAAPSPTCRP GAAGR in AAA37374. Ref.1
	Sequence conflict	356 – 359	4	GNCA → ATAREARGCGTALGRPPGWR PRGWFRVAGSLGCPGRASQD in AAA37374. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P53566 [UniParc]. Last modified January 23, 2002. Version 2. Checksum: 1E6CC09A330BEFEF	FASTA	359	37,430
	10 20 30 40 50 60 MESADFYEVE PRPPMSSHLQ SPPHAPSNAA FGFPRGAGPA PPPAPPAAPE PLGGICEHET 70 80 90 100 110 120 SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAAGPAGG GGDFDYPGAP AGPGGAVMSA 130 140 150 160 170 180 GAHGPPPGYG CAAAGYLDGR LEPLYERVGA PALRPLVIKQ EPREEDEAKQ LALAGLFPYQ 190 200 210 220 230 240 PPPPPPPPHP HASPAHLAAP HLQFQIAHCG QTTMHLQPGH PTPPPTPVPS PHAAPALGAA 250 260 270 280 290 300 GLPGPGSALK GLAGAHPDLR TGGGGGGSGA GAGKAKKSVD KNSNEYRVRR ERNNIAVRKS 310 320 330 340 350 RDKAKQRNVE TQQKVLELTS DNDRLRKRVE QLSRELDTLR GIFRQLPESS LVKAMGNCA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"CCAAT/enhancer binding protein gene promoter: binding of nuclear factors during differentiation of 3T3-L1 preadipocytes." Christy R.J., Kaestner K.H., Geiman D.E., Lane M.D. Proc. Natl. Acad. Sci. U.S.A. 88:2593-2597(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
[3]	"Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex." Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M. Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PRDM16.
[4]	"Regulation of adipocyte differentiation by the zinc finger protein ZNF638." Meruvu S., Hugendubler L., Mueller E. J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZNF638.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62362 Genomic DNA. Translation: AAA37374.1. BC011118 mRNA. Translation: AAH11118.1. BC028890 mRNA. Translation: AAH28890.1. BC051102 mRNA. Translation: AAH51102.1. BC058161 mRNA. Translation: AAH58161.1.
IPI	IPI00132435.
PIR	I49575.
RefSeq	NP_031704.2. NM_007678.3.
UniGene	Mm.349667.
3D structure databases
ProteinModelPortal	P53566.
SMR	P53566. Positions 282-341.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1529126.
PTM databases
PhosphoSite	P53566.
Proteomic databases
PaxDb	P53566.
PRIDE	P53566.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000042985; ENSMUSP00000096129; ENSMUSG00000034957.
GeneID	12606.
KEGG	mmu:12606.
UCSC	uc009gjl.2. mouse.
Organism-specific databases
CTD	1050.
MGI	MGI:99480. Cebpa.
Phylogenomic databases
eggNOG	NOG244516.
GeneTree	ENSGT00530000063192.
HOGENOM	HOG000013112.
HOVERGEN	HBG050879.
InParanoid	P53566.
KO	K09055.
OMA	DICENEN.
OrthoDB	EOG4M65JR.
Enzyme and pathway databases
Reactome	REACT_127416. Developmental Biology. REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
Gene expression databases
Bgee	P53566.
CleanEx	MM_CEBPA.
Genevestigator	P53566.
GermOnline	ENSMUSG00000034957. Mus musculus.
Family and domain databases
InterPro	IPR004827. bZIP. IPR016468. CCAAT/enhancer-binding. [Graphical view]
Pfam	PF07716. bZIP_2. 1 hit. [Graphical view]
PIRSF	PIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	281750.
SOURCE	Search...

Entry information

Entry name

CEBPA_MOUSE

Accession

Primary (citable) accession number: P53566
Secondary accession number(s): Q91XB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2002
Last modified:	May 29, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program