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P53566

- CEBPA_MOUSE

UniProt

P53566 - CEBPA_MOUSE

Protein

CCAAT/enhancer-binding protein alpha

Gene

Cebpa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (23 Jan 2002)
      Previous versions | rss
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    Functioni

    C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: UniProtKB
    3. protein homodimerization activity Source: MGI
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: NTNU_SB
    6. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    7. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    8. transcription factor binding Source: UniProtKB
    9. transcription regulatory region DNA binding Source: MGI

    GO - Biological processi

    1. acute-phase response Source: Ensembl
    2. brown fat cell differentiation Source: MGI
    3. cell maturation Source: MGI
    4. cellular response to lithium ion Source: MGI
    5. cellular response to organic cyclic compound Source: MGI
    6. cholesterol metabolic process Source: MGI
    7. cytokine-mediated signaling pathway Source: UniProtKB
    8. embryonic placenta development Source: MGI
    9. fat cell differentiation Source: MGI
    10. inner ear development Source: MGI
    11. liver development Source: MGI
    12. lung development Source: MGI
    13. macrophage differentiation Source: MGI
    14. mitochondrion organization Source: MGI
    15. myeloid cell differentiation Source: UniProtKB
    16. negative regulation of cell proliferation Source: MGI
    17. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    18. organ regeneration Source: Ensembl
    19. positive regulation of fat cell differentiation Source: MGI
    20. positive regulation of osteoblast differentiation Source: MGI
    21. positive regulation of transcription, DNA-templated Source: MGI
    22. positive regulation of transcription from RNA polymerase III promoter Source: Ensembl
    23. positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    24. regulation of cell proliferation Source: MGI
    25. regulation of transcription, DNA-templated Source: MGI
    26. regulation of transcription from RNA polymerase II promoter Source: MGI
    27. response to glucocorticoid Source: Ensembl
    28. response to vitamin B2 Source: Ensembl
    29. transcription, DNA-templated Source: UniProtKB
    30. transcription from RNA polymerase II promoter Source: GOC
    31. urea cycle Source: MGI
    32. white fat cell differentiation Source: MGI

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CCAAT/enhancer-binding protein alpha
    Short name:
    C/EBP alpha
    Gene namesi
    Name:Cebpa
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:99480. Cebpa.

    Subcellular locationi

    GO - Cellular componenti

    1. nuclear matrix Source: Ensembl
    2. nucleoplasm Source: Reactome
    3. nucleus Source: MGI
    4. Rb-E2F complex Source: Ensembl
    5. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 359359CCAAT/enhancer-binding protein alphaPRO_0000076614Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei159 – 1591N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP53566.
    PRIDEiP53566.

    PTM databases

    PhosphoSiteiP53566.

    Expressioni

    Gene expression databases

    BgeeiP53566.
    CleanExiMM_CEBPA.
    GenevestigatoriP53566.

    Interactioni

    Subunit structurei

    Interacts with UBN1 By similarity. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with PRDM16. Interacts with ZNF638; this interaction increases transcriptional activation.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Foxo1Q9R1E05EBI-2644207,EBI-1371343

    Protein-protein interaction databases

    BioGridi198667. 17 interactions.
    DIPiDIP-44054N.
    IntActiP53566. 3 interactions.
    MINTiMINT-1529126.

    Structurei

    3D structure databases

    ProteinModelPortaliP53566.
    SMRiP53566. Positions 282-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini283 – 34664bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni287 – 31428Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni318 – 34629Leucine-zipperPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi99 – 1024Poly-Gly
    Compositional biasi181 – 19010Poly-Pro
    Compositional biasi262 – 27110Poly-Gly

    Sequence similaritiesi

    Belongs to the bZIP family. C/EBP subfamily.Curated
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG244516.
    GeneTreeiENSGT00530000063192.
    HOGENOMiHOG000013112.
    HOVERGENiHBG050879.
    InParanoidiP53566.
    KOiK09055.
    OMAiPPPGYGC.
    OrthoDBiEOG7R56TQ.
    PhylomeDBiP53566.
    TreeFamiTF105008.

    Family and domain databases

    InterProiIPR004827. bZIP.
    IPR016468. CCAAT/enhancer-binding.
    [Graphical view]
    PfamiPF07716. bZIP_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005879. CCAAT/enhancer-binding. 1 hit.
    SMARTiSM00338. BRLZ. 1 hit.
    [Graphical view]
    PROSITEiPS50217. BZIP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESADFYEVE PRPPMSSHLQ SPPHAPSNAA FGFPRGAGPA PPPAPPAAPE    50
    PLGGICEHET SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAAGPAGG 100
    GGDFDYPGAP AGPGGAVMSA GAHGPPPGYG CAAAGYLDGR LEPLYERVGA 150
    PALRPLVIKQ EPREEDEAKQ LALAGLFPYQ PPPPPPPPHP HASPAHLAAP 200
    HLQFQIAHCG QTTMHLQPGH PTPPPTPVPS PHAAPALGAA GLPGPGSALK 250
    GLAGAHPDLR TGGGGGGSGA GAGKAKKSVD KNSNEYRVRR ERNNIAVRKS 300
    RDKAKQRNVE TQQKVLELTS DNDRLRKRVE QLSRELDTLR GIFRQLPESS 350
    LVKAMGNCA 359
    Length:359
    Mass (Da):37,430
    Last modified:January 23, 2002 - v2
    Checksum:i1E6CC09A330BEFEF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti30 – 5425AFGFP…EPLGG → RLWLSPGRGPRAAPSPTCRP GAAGR in AAA37374. (PubMed:2006196)CuratedAdd
    BLAST
    Sequence conflicti356 – 3594GNCA → ATAREARGCGTALGRPPGWR PRGWFRVAGSLGCPGRASQD in AAA37374. (PubMed:2006196)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62362 Genomic DNA. Translation: AAA37374.1.
    BC011118 mRNA. Translation: AAH11118.1.
    BC028890 mRNA. Translation: AAH28890.1.
    BC051102 mRNA. Translation: AAH51102.1.
    BC058161 mRNA. Translation: AAH58161.1.
    CCDSiCCDS21145.1.
    PIRiI49575.
    RefSeqiNP_031704.2. NM_007678.3.
    UniGeneiMm.349667.

    Genome annotation databases

    EnsembliENSMUST00000042985; ENSMUSP00000096129; ENSMUSG00000034957.
    GeneIDi12606.
    KEGGimmu:12606.
    UCSCiuc009gjl.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M62362 Genomic DNA. Translation: AAA37374.1 .
    BC011118 mRNA. Translation: AAH11118.1 .
    BC028890 mRNA. Translation: AAH28890.1 .
    BC051102 mRNA. Translation: AAH51102.1 .
    BC058161 mRNA. Translation: AAH58161.1 .
    CCDSi CCDS21145.1.
    PIRi I49575.
    RefSeqi NP_031704.2. NM_007678.3.
    UniGenei Mm.349667.

    3D structure databases

    ProteinModelPortali P53566.
    SMRi P53566. Positions 282-341.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198667. 17 interactions.
    DIPi DIP-44054N.
    IntActi P53566. 3 interactions.
    MINTi MINT-1529126.

    PTM databases

    PhosphoSitei P53566.

    Proteomic databases

    PaxDbi P53566.
    PRIDEi P53566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000042985 ; ENSMUSP00000096129 ; ENSMUSG00000034957 .
    GeneIDi 12606.
    KEGGi mmu:12606.
    UCSCi uc009gjl.2. mouse.

    Organism-specific databases

    CTDi 1050.
    MGIi MGI:99480. Cebpa.

    Phylogenomic databases

    eggNOGi NOG244516.
    GeneTreei ENSGT00530000063192.
    HOGENOMi HOG000013112.
    HOVERGENi HBG050879.
    InParanoidi P53566.
    KOi K09055.
    OMAi PPPGYGC.
    OrthoDBi EOG7R56TQ.
    PhylomeDBi P53566.
    TreeFami TF105008.

    Enzyme and pathway databases

    Reactomei REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

    Miscellaneous databases

    NextBioi 281750.
    PROi P53566.
    SOURCEi Search...

    Gene expression databases

    Bgeei P53566.
    CleanExi MM_CEBPA.
    Genevestigatori P53566.

    Family and domain databases

    InterProi IPR004827. bZIP.
    IPR016468. CCAAT/enhancer-binding.
    [Graphical view ]
    Pfami PF07716. bZIP_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005879. CCAAT/enhancer-binding. 1 hit.
    SMARTi SM00338. BRLZ. 1 hit.
    [Graphical view ]
    PROSITEi PS50217. BZIP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "CCAAT/enhancer binding protein gene promoter: binding of nuclear factors during differentiation of 3T3-L1 preadipocytes."
      Christy R.J., Kaestner K.H., Geiman D.E., Lane M.D.
      Proc. Natl. Acad. Sci. U.S.A. 88:2593-2597(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    3. "Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex."
      Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M.
      Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRDM16.
    4. "Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
      Meruvu S., Hugendubler L., Mueller E.
      J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZNF638.

    Entry informationi

    Entry nameiCEBPA_MOUSE
    AccessioniPrimary (citable) accession number: P53566
    Secondary accession number(s): Q91XB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2002
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3