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P53566

- CEBPA_MOUSE

UniProt

P53566 - CEBPA_MOUSE

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Protein
CCAAT/enhancer-binding protein alpha
Gene
Cebpa
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  3. protein binding Source: UniProtKB
  4. protein homodimerization activity Source: MGI
  5. sequence-specific DNA binding Source: InterPro
  6. sequence-specific DNA binding transcription factor activity Source: UniProtKB
  7. transcription factor binding Source: UniProtKB
  8. transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  1. acute-phase response Source: Ensembl
  2. brown fat cell differentiation Source: MGI
  3. cell maturation Source: MGI
  4. cellular response to lithium ion Source: MGI
  5. cellular response to organic cyclic compound Source: MGI
  6. cholesterol metabolic process Source: MGI
  7. cytokine-mediated signaling pathway Source: UniProtKB
  8. embryonic placenta development Source: MGI
  9. fat cell differentiation Source: MGI
  10. inner ear development Source: MGI
  11. liver development Source: MGI
  12. lung development Source: MGI
  13. macrophage differentiation Source: MGI
  14. mitochondrion organization Source: MGI
  15. myeloid cell differentiation Source: UniProtKB
  16. negative regulation of cell proliferation Source: MGI
  17. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  18. organ regeneration Source: Ensembl
  19. positive regulation of fat cell differentiation Source: MGI
  20. positive regulation of osteoblast differentiation Source: MGI
  21. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  22. positive regulation of transcription from RNA polymerase III promoter Source: Ensembl
  23. positive regulation of transcription, DNA-templated Source: MGI
  24. regulation of cell proliferation Source: MGI
  25. regulation of transcription from RNA polymerase II promoter Source: MGI
  26. regulation of transcription, DNA-templated Source: MGI
  27. response to glucocorticoid Source: Ensembl
  28. response to vitamin B2 Source: Ensembl
  29. transcription from RNA polymerase II promoter Source: GOC
  30. transcription, DNA-templated Source: UniProtKB
  31. urea cycle Source: MGI
  32. white fat cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
CCAAT/enhancer-binding protein alpha
Short name:
C/EBP alpha
Gene namesi
Name:Cebpa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:99480. Cebpa.

Subcellular locationi

GO - Cellular componenti

  1. Rb-E2F complex Source: Ensembl
  2. nuclear matrix Source: Ensembl
  3. nucleoplasm Source: Reactome
  4. nucleus Source: MGI
  5. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359CCAAT/enhancer-binding protein alpha
PRO_0000076614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53566.
PRIDEiP53566.

PTM databases

PhosphoSiteiP53566.

Expressioni

Gene expression databases

BgeeiP53566.
CleanExiMM_CEBPA.
GenevestigatoriP53566.

Interactioni

Subunit structurei

Interacts with UBN1 By similarity. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with PRDM16. Interacts with ZNF638; this interaction increases transcriptional activation.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Foxo1Q9R1E05EBI-2644207,EBI-1371343

Protein-protein interaction databases

BioGridi198667. 17 interactions.
IntActiP53566. 3 interactions.
MINTiMINT-1529126.

Structurei

3D structure databases

ProteinModelPortaliP53566.
SMRiP53566. Positions 282-341.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini283 – 34664bZIP
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni287 – 31428Basic motif By similarity
Add
BLAST
Regioni318 – 34629Leucine-zipper By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi99 – 1024Poly-Gly
Compositional biasi181 – 19010Poly-Pro
Compositional biasi262 – 27110Poly-Gly

Sequence similaritiesi

Belongs to the bZIP family. C/EBP subfamily.

Phylogenomic databases

eggNOGiNOG244516.
GeneTreeiENSGT00530000063192.
HOGENOMiHOG000013112.
HOVERGENiHBG050879.
InParanoidiP53566.
KOiK09055.
OMAiPPPGYGC.
OrthoDBiEOG7R56TQ.
PhylomeDBiP53566.
TreeFamiTF105008.

Family and domain databases

InterProiIPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view]
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53566-1 [UniParc]FASTAAdd to Basket

« Hide

MESADFYEVE PRPPMSSHLQ SPPHAPSNAA FGFPRGAGPA PPPAPPAAPE    50
PLGGICEHET SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAAGPAGG 100
GGDFDYPGAP AGPGGAVMSA GAHGPPPGYG CAAAGYLDGR LEPLYERVGA 150
PALRPLVIKQ EPREEDEAKQ LALAGLFPYQ PPPPPPPPHP HASPAHLAAP 200
HLQFQIAHCG QTTMHLQPGH PTPPPTPVPS PHAAPALGAA GLPGPGSALK 250
GLAGAHPDLR TGGGGGGSGA GAGKAKKSVD KNSNEYRVRR ERNNIAVRKS 300
RDKAKQRNVE TQQKVLELTS DNDRLRKRVE QLSRELDTLR GIFRQLPESS 350
LVKAMGNCA 359
Length:359
Mass (Da):37,430
Last modified:January 23, 2002 - v2
Checksum:i1E6CC09A330BEFEF
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti30 – 5425AFGFP…EPLGG → RLWLSPGRGPRAAPSPTCRP GAAGR in AAA37374. 1 Publication
Add
BLAST
Sequence conflicti356 – 3594GNCA → ATAREARGCGTALGRPPGWR PRGWFRVAGSLGCPGRASQD in AAA37374. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62362 Genomic DNA. Translation: AAA37374.1.
BC011118 mRNA. Translation: AAH11118.1.
BC028890 mRNA. Translation: AAH28890.1.
BC051102 mRNA. Translation: AAH51102.1.
BC058161 mRNA. Translation: AAH58161.1.
CCDSiCCDS21145.1.
PIRiI49575.
RefSeqiNP_031704.2. NM_007678.3.
UniGeneiMm.349667.

Genome annotation databases

EnsembliENSMUST00000042985; ENSMUSP00000096129; ENSMUSG00000034957.
GeneIDi12606.
KEGGimmu:12606.
UCSCiuc009gjl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62362 Genomic DNA. Translation: AAA37374.1 .
BC011118 mRNA. Translation: AAH11118.1 .
BC028890 mRNA. Translation: AAH28890.1 .
BC051102 mRNA. Translation: AAH51102.1 .
BC058161 mRNA. Translation: AAH58161.1 .
CCDSi CCDS21145.1.
PIRi I49575.
RefSeqi NP_031704.2. NM_007678.3.
UniGenei Mm.349667.

3D structure databases

ProteinModelPortali P53566.
SMRi P53566. Positions 282-341.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198667. 17 interactions.
IntActi P53566. 3 interactions.
MINTi MINT-1529126.

PTM databases

PhosphoSitei P53566.

Proteomic databases

PaxDbi P53566.
PRIDEi P53566.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000042985 ; ENSMUSP00000096129 ; ENSMUSG00000034957 .
GeneIDi 12606.
KEGGi mmu:12606.
UCSCi uc009gjl.2. mouse.

Organism-specific databases

CTDi 1050.
MGIi MGI:99480. Cebpa.

Phylogenomic databases

eggNOGi NOG244516.
GeneTreei ENSGT00530000063192.
HOGENOMi HOG000013112.
HOVERGENi HBG050879.
InParanoidi P53566.
KOi K09055.
OMAi PPPGYGC.
OrthoDBi EOG7R56TQ.
PhylomeDBi P53566.
TreeFami TF105008.

Enzyme and pathway databases

Reactomei REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

NextBioi 281750.
PROi P53566.
SOURCEi Search...

Gene expression databases

Bgeei P53566.
CleanExi MM_CEBPA.
Genevestigatori P53566.

Family and domain databases

InterProi IPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view ]
Pfami PF07716. bZIP_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMARTi SM00338. BRLZ. 1 hit.
[Graphical view ]
PROSITEi PS50217. BZIP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "CCAAT/enhancer binding protein gene promoter: binding of nuclear factors during differentiation of 3T3-L1 preadipocytes."
    Christy R.J., Kaestner K.H., Geiman D.E., Lane M.D.
    Proc. Natl. Acad. Sci. U.S.A. 88:2593-2597(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex."
    Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M.
    Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRDM16.
  4. "Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
    Meruvu S., Hugendubler L., Mueller E.
    J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZNF638.

Entry informationi

Entry nameiCEBPA_MOUSE
AccessioniPrimary (citable) accession number: P53566
Secondary accession number(s): Q91XB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2002
Last modified: September 3, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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