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P53566 (CEBPA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CCAAT/enhancer-binding protein alpha

Short name=C/EBP alpha
Gene names
Name:Cebpa
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Subunit structure

Interacts with UBN1 By similarity. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with PRDM16. Interacts with ZNF638; this interaction increases transcriptional activation. Ref.3 Ref.4

Subcellular location

Nucleus.

Sequence similarities

Belongs to the bZIP family. C/EBP subfamily.

Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacute-phase response

Inferred from electronic annotation. Source: Ensembl

brown fat cell differentiation

Inferred from direct assay PubMed 18492766. Source: MGI

cell maturation

Inferred from mutant phenotype PubMed 16467360. Source: MGI

cellular response to lithium ion

Inferred from direct assay PubMed 15673614. Source: MGI

cellular response to organic cyclic compound

Inferred from direct assay PubMed 12055200. Source: MGI

cholesterol metabolic process

Inferred from mutant phenotype PubMed 18974039. Source: MGI

cytokine-mediated signaling pathway

Non-traceable author statement PubMed 15664994. Source: UniProtKB

embryonic placenta development

Inferred from genetic interaction PubMed 15509779. Source: MGI

fat cell differentiation

Inferred from direct assay PubMed 12037571PubMed 12502791. Source: MGI

inner ear development

Inferred from direct assay PubMed 21795542. Source: MGI

liver development

Inferred from mutant phenotype PubMed 17021047. Source: MGI

lung development

Inferred from mutant phenotype PubMed 16467360. Source: MGI

macrophage differentiation

Inferred from mutant phenotype PubMed 15073037. Source: MGI

mitochondrion organization

Inferred from mutant phenotype PubMed 11940593. Source: MGI

myeloid cell differentiation

Inferred from direct assay PubMed 15664994. Source: UniProtKB

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 15073037. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10859308. Source: MGI

organ regeneration

Inferred from electronic annotation. Source: Ensembl

positive regulation of fat cell differentiation

Inferred from direct assay PubMed 10937998. Source: MGI

positive regulation of osteoblast differentiation

Inferred from direct assay PubMed 15175325. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18632661. Source: MGI

positive regulation of transcription from RNA polymerase III promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 17213233. Source: MGI

regulation of cell proliferation

Inferred from mutant phenotype PubMed 17021047. Source: MGI

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 18632661. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 10075730. Source: MGI

response to glucocorticoid

Inferred from electronic annotation. Source: Ensembl

response to vitamin B2

Inferred from electronic annotation. Source: Ensembl

transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 10859308. Source: GOC

transcription, DNA-templated

Non-traceable author statement PubMed 15664994PubMed 7575576. Source: UniProtKB

urea cycle

Inferred from direct assay PubMed 17213233. Source: MGI

white fat cell differentiation

Inferred from mutant phenotype PubMed 15130516. Source: MGI

   Cellular_componentRb-E2F complex

Inferred from electronic annotation. Source: Ensembl

nuclear matrix

Inferred from electronic annotation. Source: Ensembl

nucleoplasm

Traceable author statement. Source: Reactome

nucleus

Inferred from direct assay PubMed 11940593PubMed 15073037PubMed 15519652PubMed 18824566PubMed 21669876. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 12861022. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 10510303PubMed 12821655PubMed 15073037PubMed 16912278PubMed 17213233. Source: MGI

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 10859308. Source: MGI

protein homodimerization activity

Inferred from direct assay PubMed 12821655. Source: MGI

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Non-traceable author statement PubMed 15664994. Source: UniProtKB

transcription factor binding

Inferred from sequence or structural similarity. Source: UniProtKB

transcription regulatory region DNA binding

Inferred from direct assay PubMed 18632661. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Foxo1Q9R1E05EBI-2644207,EBI-1371343

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359CCAAT/enhancer-binding protein alpha
PRO_0000076614

Regions

Domain283 – 34664bZIP
Region287 – 31428Basic motif By similarity
Region318 – 34629Leucine-zipper By similarity
Compositional bias99 – 1024Poly-Gly
Compositional bias181 – 19010Poly-Pro
Compositional bias262 – 27110Poly-Gly

Amino acid modifications

Modified residue1591N6-acetyllysine By similarity

Experimental info

Sequence conflict30 – 5425AFGFP…EPLGG → RLWLSPGRGPRAAPSPTCRP GAAGR in AAA37374. Ref.1
Sequence conflict356 – 3594GNCA → ATAREARGCGTALGRPPGWR PRGWFRVAGSLGCPGRASQD in AAA37374. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P53566 [UniParc].

Last modified January 23, 2002. Version 2.
Checksum: 1E6CC09A330BEFEF

FASTA35937,430
        10         20         30         40         50         60 
MESADFYEVE PRPPMSSHLQ SPPHAPSNAA FGFPRGAGPA PPPAPPAAPE PLGGICEHET 

        70         80         90        100        110        120 
SIDISAYIDP AAFNDEFLAD LFQHSRQQEK AKAAAGPAGG GGDFDYPGAP AGPGGAVMSA 

       130        140        150        160        170        180 
GAHGPPPGYG CAAAGYLDGR LEPLYERVGA PALRPLVIKQ EPREEDEAKQ LALAGLFPYQ 

       190        200        210        220        230        240 
PPPPPPPPHP HASPAHLAAP HLQFQIAHCG QTTMHLQPGH PTPPPTPVPS PHAAPALGAA 

       250        260        270        280        290        300 
GLPGPGSALK GLAGAHPDLR TGGGGGGSGA GAGKAKKSVD KNSNEYRVRR ERNNIAVRKS 

       310        320        330        340        350 
RDKAKQRNVE TQQKVLELTS DNDRLRKRVE QLSRELDTLR GIFRQLPESS LVKAMGNCA 

« Hide

References

« Hide 'large scale' references
[1]"CCAAT/enhancer binding protein gene promoter: binding of nuclear factors during differentiation of 3T3-L1 preadipocytes."
Christy R.J., Kaestner K.H., Geiman D.E., Lane M.D.
Proc. Natl. Acad. Sci. U.S.A. 88:2593-2597(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[3]"Initiation of myoblast to brown fat switch by a PRDM16-C/EBP-beta transcriptional complex."
Kajimura S., Seale P., Kubota K., Lunsford E., Frangioni J.V., Gygi S.P., Spiegelman B.M.
Nature 460:1154-1158(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PRDM16.
[4]"Regulation of adipocyte differentiation by the zinc finger protein ZNF638."
Meruvu S., Hugendubler L., Mueller E.
J. Biol. Chem. 286:26516-26523(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZNF638.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M62362 Genomic DNA. Translation: AAA37374.1.
BC011118 mRNA. Translation: AAH11118.1.
BC028890 mRNA. Translation: AAH28890.1.
BC051102 mRNA. Translation: AAH51102.1.
BC058161 mRNA. Translation: AAH58161.1.
PIRI49575.
RefSeqNP_031704.2. NM_007678.3.
UniGeneMm.349667.

3D structure databases

ProteinModelPortalP53566.
SMRP53566. Positions 282-341.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198667. 17 interactions.
IntActP53566. 3 interactions.
MINTMINT-1529126.

PTM databases

PhosphoSiteP53566.

Proteomic databases

PaxDbP53566.
PRIDEP53566.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000042985; ENSMUSP00000096129; ENSMUSG00000034957.
GeneID12606.
KEGGmmu:12606.
UCSCuc009gjl.2. mouse.

Organism-specific databases

CTD1050.
MGIMGI:99480. Cebpa.

Phylogenomic databases

eggNOGNOG244516.
GeneTreeENSGT00530000063192.
HOGENOMHOG000013112.
HOVERGENHBG050879.
InParanoidP53566.
KOK09055.
OMAPPPGYGC.
OrthoDBEOG7R56TQ.
PhylomeDBP53566.
TreeFamTF105008.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Gene expression databases

BgeeP53566.
CleanExMM_CEBPA.
GenevestigatorP53566.

Family and domain databases

InterProIPR004827. bZIP.
IPR016468. CCAAT/enhancer-binding.
[Graphical view]
PfamPF07716. bZIP_2. 1 hit.
[Graphical view]
PIRSFPIRSF005879. CCAAT/enhancer-binding. 1 hit.
SMARTSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281750.
PROP53566.
SOURCESearch...

Entry information

Entry nameCEBPA_MOUSE
AccessionPrimary (citable) accession number: P53566
Secondary accession number(s): Q91XB6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2002
Last modified: April 16, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot