Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein cut-like 1

Gene

Cux1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.3 Publications

Miscellaneous

Asn-1285 may participate in regulating DNA-binding activity by promoting homo- and heterodimerization.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi540 – 627CUT 1PROSITE-ProRule annotationAdd BLAST88
DNA bindingi929 – 1016CUT 2PROSITE-ProRule annotationAdd BLAST88
DNA bindingi1112 – 1199CUT 3PROSITE-ProRule annotationAdd BLAST88
DNA bindingi1239 – 1298HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • chromatin binding Source: MGI
  • DNA binding Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProtKB
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific DNA binding Source: MGI

GO - Biological processi

  • Golgi vesicle transport Source: GO_Central
  • inner ear auditory receptor cell differentiation Source: MGI
  • kidney development Source: MGI
  • lung development Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of dendrite morphogenesis Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein cut-like 1
Alternative name(s):
CCAAT displacement protein
Short name:
CDP
Homeobox protein cux-1
Gene namesi
Name:Cux1
Synonyms:Cutl1, Cux, Kiaa4047
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88568 Cux1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002023941 – 1515Homeobox protein cut-like 1Add BLAST1515

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei427PhosphoserineCombined sources1
Modified residuei761PhosphoserineBy similarity1
Cross-linki783Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki809Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki840Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei904PhosphoserineBy similarity1
Modified residuei1054PhosphoserineBy similarity1
Modified residuei1064PhosphoserineBy similarity1
Modified residuei1265PhosphoserineBy similarity1
Cross-linki1279Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei1332PhosphoserineCombined sources1
Modified residuei1468PhosphoserineBy similarity1
Modified residuei1496PhosphoserineBy similarity1
Modified residuei1506PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PeptideAtlasiP53564
PRIDEiP53564

PTM databases

iPTMnetiP53564
PhosphoSitePlusiP53564

Expressioni

Tissue specificityi

Isoform 6 is testis-specific where it is expressed in germ cells.1 Publication

Developmental stagei

In postpubertal testis, isoform 6 is expressed from stages IV-V of spermatogenesis in the outer layer of round spermatids. Expression continues through stages VI-VII but no expression is detected in stages IX-XI. In prepubertal testis, isoform 6 is expressed in post-meiotic germ cells at the round spermatid stage.1 Publication

Gene expression databases

CleanExiMM_CUX1

Interactioni

Subunit structurei

Interacts with BANP. Interacts with SATB1 (via DNA-binding domains); the interaction inhibits the attachment of both proteins to DNA.2 Publications

Protein-protein interaction databases

BioGridi198981, 4 interactors
IntActiP53564, 5 interactors
MINTiP53564

Structurei

3D structure databases

ProteinModelPortaliP53564
SMRiP53564
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili56 – 361Sequence analysisAdd BLAST306

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1358 – 1463Ala-richAdd BLAST106

Sequence similaritiesi

Belongs to the CUT homeobox family.Curated

Keywords - Domaini

Coiled coil, Homeobox, Repeat

Phylogenomic databases

HOGENOMiHOG000143386
HOVERGENiHBG051268
InParanoidiP53564
KOiK09313
PhylomeDBiP53564

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR003350 CUT_dom
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
PfamiView protein in Pfam
PF02376 CUT, 3 hits
PF00046 Homeobox, 1 hit
SMARTiView protein in SMART
SM01109 CUT, 3 hits
SM00389 HOX, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 3 hits
PROSITEiView protein in PROSITE
PS51042 CUT, 3 hits
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 5 (identifier: P53564-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLCVAGAKLK RELDATATVL ANRQDESEQS RKRLIEQSRE FKKNTPEDLR
60 70 80 90 100
KQVAPLLKSF QGEIDALSKR SKEAEAAFLT VYKRLIDVPD PVPALDVGQQ
110 120 130 140 150
LEIKVQRLHD IETENQKLRE TLEEYNKEFA EVKNQEVTIK ALKEKIREYE
160 170 180 190 200
QTLKSQAETI ALEKEQKLQN DFAEKERKLQ ETQMSTTSKL EEAEHKLQTL
210 220 230 240 250
QTALEKTRTE LFDLKTKYDE ETTAKADEIE MIMTDLERAN QRAEVAQREA
260 270 280 290 300
ETLREQLSSA NHSLQLASQI QKAPDVAIEV LTRSSLEVEL AAKEREIAQL
310 320 330 340 350
VEDVQRLQAS LTKLRENSAS QISQLEQQLN AKNSTLKQLE EKLKGQADYE
360 370 380 390 400
EVKKELNTLK SMEFAPSEGA GTQDSTKPLE VLLLEKNRSL QSENATLRIS
410 420 430 440 450
NSDLSGSARR KGRDQPESRR PGPLPASPPP QLPRNTGEQV SNTNGTHHFS
460 470 480 490 500
PAGLSQDFFS SNLASPSLPL ASTGKFALNS LLQRQLMQSF YSKAMQEAGS
510 520 530 540 550
TSTIFSTGPY STNSISSPSP LQQSPDVNGM APSPSQSESA GSISEGEEID
560 570 580 590 600
TAEIARQVKE QLIKHNIGQR IFGHYVLGLS QGSVSEILAR PKPWNKLTVR
610 620 630 640 650
GKEPFHKMKQ FLSDEQNILA LRSIQGRQRE NPGQSLNRLF QEVPKRRNRS
660 670 680 690 700
EGNITTRIRA SETGSDEAIK SILEQAKREL QVQKTAEPVQ TSSTSSSGNS
710 720 730 740 750
DDAIRSILQQ ARREMEAQQA ALDPALKPAP LSQPDLTILT PKHLSASPMS
760 770 780 790 800
TVSTYPPLAI SLKKTPAAPE TSTAALPSAP ALKKEAQDVP TLDPPGSADA
810 820 830 840 850
AQGVLRPMKS ELVRGSTWKD PWWSPIQPER RNLTSSEETK ADETTASGKE
860 870 880 890 900
RAGSSQPRAE RSQLQGPSAS AEYWKEWPSA ESPYSQSSEL SLTGASRSET
910 920 930 940 950
PQNSPLPSSP IVPMAKPAKP SVPPLTPEQY EVYMYQEVDT IELTRQVKEK
960 970 980 990 1000
LAKNGICQRI FGEKVLGLSQ GSVSDMLSRP KPWSKLTQKG REPFIRMQLW
1010 1020 1030 1040 1050
LNGELGQGVL PVQGQQQGPV LHSVASLQDP LQQGCVSSES TPKTSASCSP
1060 1070 1080 1090 1100
APESPMSSSE SVKSLTELVQ QPCPAIETSK EGKPPEPSDP PASDSQPTTP
1110 1120 1130 1140 1150
LPLSGHSALS IQELVAMSPE LDTYGITKRV KEVLTDNNLG QRLFGETILG
1160 1170 1180 1190 1200
LTQGSVSDLL ARPKPWHKLS LKGREPFVRM QLWLNDPNNV EKLMDMKRME
1210 1220 1230 1240 1250
KKAYMKRRHS SVSDSQPCEP PSVGIDYSQG ASPQPQHQLK KPRVVLAPEE
1260 1270 1280 1290 1300
KEALKRAYQQ KPYPSPKTIE ELATQLNLKT STVINWFHNY RSRIRRELFI
1310 1320 1330 1340 1350
EEIQAGSQGQ AGASDSPSAR SSRAAPSSEG DSCDGVEATD AEEPGGNIVA
1360 1370 1380 1390 1400
TKSQGGLAEV AAAPADREEA TQPAEKAKAQ PLCSGTPGQD DGEDASRPRP
1410 1420 1430 1440 1450
LPEGLADAPA PVPSLAAPAA GEDAATSATA PATATEAPGA ARAGPAERSS
1460 1470 1480 1490 1500
ALPSTSAPAN APARRPSSLQ SLFGLPEAAG ARDNPVRKKK AANLNSIIHR
1510
LEKAASREEP IEWEF
Length:1,515
Mass (Da):165,596
Last modified:September 27, 2005 - v3
Checksum:i72BC6E8D8ECD78DE
GO
Isoform 2 (identifier: P53564-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     406-507: Missing.

Show »
Length:1,413
Mass (Da):154,697
Checksum:i5C7D6BE7CA4AD3BE
GO
Isoform 3 (identifier: P53564-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MLCVAGAKLK → MAANVGSMFQYWKRFDLQQLQ
     630-651: Missing.

Note: No experimental confirmation available.
Show »
Length:1,504
Mass (Da):164,487
Checksum:i5B1EEBFC0FA74487
GO
Isoform 4 (identifier: P53564-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-183: Missing.

Show »
Length:1,332
Mass (Da):144,361
Checksum:i6A6305042CCADD61
GO
Isoform 6 (identifier: P53564-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1055: Missing.

Show »
Length:460
Mass (Da):49,021
Checksum:iB425EAF9639B9916
GO
Isoform 1 (identifier: P70403-1) [UniParc]FASTAAdd to basket
Also known as: CASP
The sequence of this isoform can be found in the external entry P70403.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:678
Mass (Da):77,269
GO

Sequence cautioni

The sequence BAD90358 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti276V → VEQ in AAK59986 (PubMed:11544187).Curated1
Sequence conflicti649R → G in CAA52922 (PubMed:7910552).Curated1
Sequence conflicti649R → G in AAD12485 (PubMed:9858552).Curated1
Sequence conflicti1480G → A in CAA52922 (PubMed:7910552).Curated1
Sequence conflicti1485P → L in AAD12485 (PubMed:9858552).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0173601 – 1055Missing in isoform 6. 1 PublicationAdd BLAST1055
Alternative sequenceiVSP_0157481 – 183Missing in isoform 4. 1 PublicationAdd BLAST183
Alternative sequenceiVSP_0157491 – 10MLCVAGAKLK → MAANVGSMFQYWKRFDLQQL Q in isoform 3. 1 Publication10
Alternative sequenceiVSP_002311406 – 507Missing in isoform 2. 1 PublicationAdd BLAST102
Alternative sequenceiVSP_015750630 – 651Missing in isoform 3. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75013 mRNA Translation: CAA52922.1
U46684 mRNA Translation: AAB41146.1
AY037807 mRNA Translation: AAK59986.1
AK220173 mRNA Translation: BAD90358.1 Different initiation.
AF004225 mRNA Translation: AAD12485.1
U46683 mRNA Translation: AAC52775.1
CCDSiCCDS71684.1 [P53564-3]
PIRiI48314
RefSeqiNP_001278162.1, NM_001291233.1
NP_001278163.1, NM_001291234.1
NP_034116.3, NM_009986.4
UniGeneiMm.320317
Mm.436792

Genome annotation databases

GeneIDi13047
KEGGimmu:13047

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCUX1_MOUSE
AccessioniPrimary (citable) accession number: P53564
Secondary accession number(s): O08994
, P70301, Q571L6, Q91ZD2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 27, 2005
Last modified: March 28, 2018
This is version 160 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health