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P53564

- CUX1_MOUSE

UniProt

P53564 - CUX1_MOUSE

Protein

Homeobox protein cut-like 1

Gene

Cux1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi540 – 62788CUT 1PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi929 – 101688CUT 2PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi1112 – 119988CUT 3PROSITE-ProRule annotationAdd
    BLAST
    DNA bindingi1239 – 129860HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. DNA binding Source: MGI
    3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
    4. RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProt

    GO - Biological processi

    1. auditory receptor cell differentiation Source: MGI
    2. kidney development Source: MGI
    3. lung development Source: MGI
    4. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    5. positive regulation of dendrite morphogenesis Source: UniProt
    6. positive regulation of transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein cut-like 1
    Alternative name(s):
    CCAAT displacement protein
    Short name:
    CDP
    Homeobox protein cux-1
    Gene namesi
    Name:Cux1
    Synonyms:Cutl1, Cux, Kiaa4047
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:88568. Cux1.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15151515Homeobox protein cut-like 1PRO_0000202394Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei761 – 7611PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53564.
    PRIDEiP53564.

    PTM databases

    PhosphoSiteiP53564.

    Expressioni

    Tissue specificityi

    Isoform 6 is testis-specific where it is expressed in germ cells.1 Publication

    Developmental stagei

    In postpubertal testis, isoform 6 is expressed from stages IV-V of spermatogenesis in the outer layer of round spermatids. Expression continues through stages VI-VII but no expression is detected in stages IX-XI. In prepubertal testis, isoform 6 is expressed in post-meiotic germ cells at the round spermatid stage.1 Publication

    Gene expression databases

    CleanExiMM_CUX1.
    GenevestigatoriP53564.

    Interactioni

    Subunit structurei

    Interacts with BANP. Interacts with SATB1 (via DNA-binding domains); the interaction inhibits the attachment of both proteins to DNA.2 Publications

    Protein-protein interaction databases

    BioGridi198981. 1 interaction.
    IntActiP53564. 3 interactions.
    MINTiMINT-1634604.

    Structurei

    3D structure databases

    ProteinModelPortaliP53564.
    SMRiP53564. Positions 545-633, 929-1019, 1111-1199.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili56 – 361306Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1358 – 1463106Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CUT homeobox family.Curated
    Contains 3 CUT DNA-binding domains.PROSITE-ProRule annotation
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Homeobox, Repeat

    Phylogenomic databases

    HOGENOMiHOG000143386.
    HOVERGENiHBG051268.
    InParanoidiP53564.
    KOiK09313.
    PhylomeDBiP53564.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 3 hits.
    InterProiIPR003350. Hmoeo_CUT.
    IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    [Graphical view]
    PfamiPF02376. CUT. 3 hits.
    PF00046. Homeobox. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 3 hits.
    PROSITEiPS51042. CUT. 3 hits.
    PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Isoform 5 (identifier: P53564-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLCVAGAKLK RELDATATVL ANRQDESEQS RKRLIEQSRE FKKNTPEDLR     50
    KQVAPLLKSF QGEIDALSKR SKEAEAAFLT VYKRLIDVPD PVPALDVGQQ 100
    LEIKVQRLHD IETENQKLRE TLEEYNKEFA EVKNQEVTIK ALKEKIREYE 150
    QTLKSQAETI ALEKEQKLQN DFAEKERKLQ ETQMSTTSKL EEAEHKLQTL 200
    QTALEKTRTE LFDLKTKYDE ETTAKADEIE MIMTDLERAN QRAEVAQREA 250
    ETLREQLSSA NHSLQLASQI QKAPDVAIEV LTRSSLEVEL AAKEREIAQL 300
    VEDVQRLQAS LTKLRENSAS QISQLEQQLN AKNSTLKQLE EKLKGQADYE 350
    EVKKELNTLK SMEFAPSEGA GTQDSTKPLE VLLLEKNRSL QSENATLRIS 400
    NSDLSGSARR KGRDQPESRR PGPLPASPPP QLPRNTGEQV SNTNGTHHFS 450
    PAGLSQDFFS SNLASPSLPL ASTGKFALNS LLQRQLMQSF YSKAMQEAGS 500
    TSTIFSTGPY STNSISSPSP LQQSPDVNGM APSPSQSESA GSISEGEEID 550
    TAEIARQVKE QLIKHNIGQR IFGHYVLGLS QGSVSEILAR PKPWNKLTVR 600
    GKEPFHKMKQ FLSDEQNILA LRSIQGRQRE NPGQSLNRLF QEVPKRRNRS 650
    EGNITTRIRA SETGSDEAIK SILEQAKREL QVQKTAEPVQ TSSTSSSGNS 700
    DDAIRSILQQ ARREMEAQQA ALDPALKPAP LSQPDLTILT PKHLSASPMS 750
    TVSTYPPLAI SLKKTPAAPE TSTAALPSAP ALKKEAQDVP TLDPPGSADA 800
    AQGVLRPMKS ELVRGSTWKD PWWSPIQPER RNLTSSEETK ADETTASGKE 850
    RAGSSQPRAE RSQLQGPSAS AEYWKEWPSA ESPYSQSSEL SLTGASRSET 900
    PQNSPLPSSP IVPMAKPAKP SVPPLTPEQY EVYMYQEVDT IELTRQVKEK 950
    LAKNGICQRI FGEKVLGLSQ GSVSDMLSRP KPWSKLTQKG REPFIRMQLW 1000
    LNGELGQGVL PVQGQQQGPV LHSVASLQDP LQQGCVSSES TPKTSASCSP 1050
    APESPMSSSE SVKSLTELVQ QPCPAIETSK EGKPPEPSDP PASDSQPTTP 1100
    LPLSGHSALS IQELVAMSPE LDTYGITKRV KEVLTDNNLG QRLFGETILG 1150
    LTQGSVSDLL ARPKPWHKLS LKGREPFVRM QLWLNDPNNV EKLMDMKRME 1200
    KKAYMKRRHS SVSDSQPCEP PSVGIDYSQG ASPQPQHQLK KPRVVLAPEE 1250
    KEALKRAYQQ KPYPSPKTIE ELATQLNLKT STVINWFHNY RSRIRRELFI 1300
    EEIQAGSQGQ AGASDSPSAR SSRAAPSSEG DSCDGVEATD AEEPGGNIVA 1350
    TKSQGGLAEV AAAPADREEA TQPAEKAKAQ PLCSGTPGQD DGEDASRPRP 1400
    LPEGLADAPA PVPSLAAPAA GEDAATSATA PATATEAPGA ARAGPAERSS 1450
    ALPSTSAPAN APARRPSSLQ SLFGLPEAAG ARDNPVRKKK AANLNSIIHR 1500
    LEKAASREEP IEWEF 1515
    Length:1,515
    Mass (Da):165,596
    Last modified:September 27, 2005 - v3
    Checksum:i72BC6E8D8ECD78DE
    GO
    Isoform 2 (identifier: P53564-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         406-507: Missing.

    Show »
    Length:1,413
    Mass (Da):154,697
    Checksum:i5C7D6BE7CA4AD3BE
    GO
    Isoform 3 (identifier: P53564-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-10: MLCVAGAKLK → MAANVGSMFQYWKRFDLQQLQ
         630-651: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,504
    Mass (Da):164,487
    Checksum:i5B1EEBFC0FA74487
    GO
    Isoform 4 (identifier: P53564-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-183: Missing.

    Show »
    Length:1,332
    Mass (Da):144,361
    Checksum:i6A6305042CCADD61
    GO
    Isoform 6 (identifier: P53564-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-1055: Missing.

    Show »
    Length:460
    Mass (Da):49,021
    Checksum:iB425EAF9639B9916
    GO
    Isoform 1 (identifier: P70403-1) [UniParc]FASTAAdd to Basket

    Also known as: CASP

    The sequence of this isoform can be found in the external entry P70403.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:678
    Mass (Da):77,269
    GO

    Sequence cautioni

    The sequence BAD90358.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti276 – 2761V → VEQ in AAK59986. (PubMed:11544187)Curated
    Sequence conflicti649 – 6491R → G in CAA52922. (PubMed:7910552)Curated
    Sequence conflicti649 – 6491R → G in AAD12485. (PubMed:9858552)Curated
    Sequence conflicti1480 – 14801G → A in CAA52922. (PubMed:7910552)Curated
    Sequence conflicti1485 – 14851P → L in AAD12485. (PubMed:9858552)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 10551055Missing in isoform 6. 1 PublicationVSP_017360Add
    BLAST
    Alternative sequencei1 – 183183Missing in isoform 4. 1 PublicationVSP_015748Add
    BLAST
    Alternative sequencei1 – 1010MLCVAGAKLK → MAANVGSMFQYWKRFDLQQL Q in isoform 3. 1 PublicationVSP_015749
    Alternative sequencei406 – 507102Missing in isoform 2. 1 PublicationVSP_002311Add
    BLAST
    Alternative sequencei630 – 65122Missing in isoform 3. 1 PublicationVSP_015750Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75013 mRNA. Translation: CAA52922.1.
    U46684 mRNA. Translation: AAB41146.1.
    AY037807 mRNA. Translation: AAK59986.1.
    AK220173 mRNA. Translation: BAD90358.1. Different initiation.
    AF004225 mRNA. Translation: AAD12485.1.
    U46683 mRNA. Translation: AAC52775.1.
    CCDSiCCDS71684.1. [P53564-3]
    PIRiI48314.
    RefSeqiNP_001278162.1. NM_001291233.1.
    NP_001278163.1. NM_001291234.1.
    NP_034116.3. NM_009986.4.
    UniGeneiMm.320317.

    Genome annotation databases

    GeneIDi13047.
    KEGGimmu:13047.
    UCSCiuc009aap.1. mouse. [P53564-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75013 mRNA. Translation: CAA52922.1 .
    U46684 mRNA. Translation: AAB41146.1 .
    AY037807 mRNA. Translation: AAK59986.1 .
    AK220173 mRNA. Translation: BAD90358.1 . Different initiation.
    AF004225 mRNA. Translation: AAD12485.1 .
    U46683 mRNA. Translation: AAC52775.1 .
    CCDSi CCDS71684.1. [P53564-3 ]
    PIRi I48314.
    RefSeqi NP_001278162.1. NM_001291233.1.
    NP_001278163.1. NM_001291234.1.
    NP_034116.3. NM_009986.4.
    UniGenei Mm.320317.

    3D structure databases

    ProteinModelPortali P53564.
    SMRi P53564. Positions 545-633, 929-1019, 1111-1199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198981. 1 interaction.
    IntActi P53564. 3 interactions.
    MINTi MINT-1634604.

    PTM databases

    PhosphoSitei P53564.

    Proteomic databases

    MaxQBi P53564.
    PRIDEi P53564.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13047.
    KEGGi mmu:13047.
    UCSCi uc009aap.1. mouse. [P53564-1 ]

    Organism-specific databases

    MGIi MGI:88568. Cux1.
    Rougei Search...

    Phylogenomic databases

    HOGENOMi HOG000143386.
    HOVERGENi HBG051268.
    InParanoidi P53564.
    KOi K09313.
    PhylomeDBi P53564.

    Miscellaneous databases

    ChiTaRSi CUX1. mouse.
    NextBioi 282952.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CUX1.
    Genevestigatori P53564.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 3 hits.
    InterProi IPR003350. Hmoeo_CUT.
    IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    [Graphical view ]
    Pfami PF02376. CUT. 3 hits.
    PF00046. Homeobox. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 3 hits.
    PROSITEi PS51042. CUT. 3 hits.
    PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The mouse homeodomain protein Phox2 regulates Ncam promoter activity in concert with Cux/CDP and is a putative determinant of neurotransmitter phenotype."
      Valarche I., Tissier-Seta J.-P., Hirsch M.R., Martinez S., Goridis C., Brunet J.-F.
      Development 119:881-896(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
      Strain: A/J and BALB/c.
      Tissue: Brain.
    2. "A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis."
      Vanden Heuvel G.B., Quaggin S.E., Igarashi P.
      Biol. Reprod. 55:731-739(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Testis.
    3. "The transcriptional repressor CDP (Cutl1) is essential for epithelial cell differentiation of the lung and the hair follicle."
      Ellis T., Gambardella L., Horcher M., Tschanz S., Capol J., Bertram P., Jochum W., Barrandon Y., Busslinger M.
      Genes Dev. 15:2307-2319(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), FUNCTION.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
      Tissue: Embryonic tail.
    5. "Cux/CDP homeoprotein is a component of NF-muNR and represses the immunoglobulin heavy chain intronic enhancer by antagonizing the bright transcription activator."
      Wang Z., Goldstein A., Zong R.-T., Lin D., Neufeld E.J., Scheuermann R.H., Tucker P.W.
      Mol. Cell. Biol. 19:284-295(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 121-1515 (ISOFORM 2), FUNCTION.
      Strain: C57BL/6N.
    6. "Expression of a cut-related homeobox gene in developing and polycystic mouse kidney."
      Vanden Heuvel G.B., Bodmer R., McConnell K.R., Nagami G.T., Igarashi P.
      Kidney Int. 50:453-461(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 762-1515 (ISOFORMS 2/3/4/5).
    7. "Homeoproteins CDP and SATB1 interact: potential for tissue-specific regulation."
      Liu J., Barnett A., Neufeld E.J., Dudley J.P.
      Mol. Cell. Biol. 19:4918-4926(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SATB1.
    8. "SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCRbeta enhancer (Ebeta)."
      Kaul-Ghanekar R., Jalota-Badhwar A., Pavithra L., Tucker P., Chattopadhyay S.
      Nucleic Acids Res. 32:4862-4875(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH BANP, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCUX1_MOUSE
    AccessioniPrimary (citable) accession number: P53564
    Secondary accession number(s): O08994
    , P70301, Q571L6, Q91ZD2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Asn-1285 may participate in regulating DNA-binding activity by promoting homo- and heterodimerization.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3