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P53564

- CUX1_MOUSE

UniProt

P53564 - CUX1_MOUSE

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Protein

Homeobox protein cut-like 1

Gene

Cux1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi540 – 62788CUT 1PROSITE-ProRule annotationAdd
BLAST
DNA bindingi929 – 101688CUT 2PROSITE-ProRule annotationAdd
BLAST
DNA bindingi1112 – 119988CUT 3PROSITE-ProRule annotationAdd
BLAST
DNA bindingi1239 – 129860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. DNA binding Source: MGI
  3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  4. RNA polymerase II regulatory region sequence-specific DNA binding Source: UniProt

GO - Biological processi

  1. auditory receptor cell differentiation Source: MGI
  2. kidney development Source: MGI
  3. lung development Source: MGI
  4. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  5. positive regulation of dendrite morphogenesis Source: UniProt
  6. positive regulation of transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein cut-like 1
Alternative name(s):
CCAAT displacement protein
Short name:
CDP
Homeobox protein cux-1
Gene namesi
Name:Cux1
Synonyms:Cutl1, Cux, Kiaa4047
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:88568. Cux1.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15151515Homeobox protein cut-like 1PRO_0000202394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei761 – 7611PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53564.
PRIDEiP53564.

PTM databases

PhosphoSiteiP53564.

Expressioni

Tissue specificityi

Isoform 6 is testis-specific where it is expressed in germ cells.1 Publication

Developmental stagei

In postpubertal testis, isoform 6 is expressed from stages IV-V of spermatogenesis in the outer layer of round spermatids. Expression continues through stages VI-VII but no expression is detected in stages IX-XI. In prepubertal testis, isoform 6 is expressed in post-meiotic germ cells at the round spermatid stage.1 Publication

Gene expression databases

CleanExiMM_CUX1.
GenevestigatoriP53564.

Interactioni

Subunit structurei

Interacts with BANP. Interacts with SATB1 (via DNA-binding domains); the interaction inhibits the attachment of both proteins to DNA.2 Publications

Protein-protein interaction databases

BioGridi198981. 1 interaction.
IntActiP53564. 3 interactions.
MINTiMINT-1634604.

Structurei

3D structure databases

ProteinModelPortaliP53564.
SMRiP53564. Positions 545-633, 929-1019, 1111-1199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili56 – 361306Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1358 – 1463106Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the CUT homeobox family.Curated
Contains 3 CUT DNA-binding domains.PROSITE-ProRule annotation
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Homeobox, Repeat

Phylogenomic databases

HOGENOMiHOG000143386.
HOVERGENiHBG051268.
InParanoidiP53564.
KOiK09313.
PhylomeDBiP53564.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 3 hits.
InterProiIPR003350. CUT_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view]
PfamiPF02376. CUT. 3 hits.
PF00046. Homeobox. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 3 hits.
PROSITEiPS51042. CUT. 3 hits.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. Align

Isoform 5 (identifier: P53564-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLCVAGAKLK RELDATATVL ANRQDESEQS RKRLIEQSRE FKKNTPEDLR
60 70 80 90 100
KQVAPLLKSF QGEIDALSKR SKEAEAAFLT VYKRLIDVPD PVPALDVGQQ
110 120 130 140 150
LEIKVQRLHD IETENQKLRE TLEEYNKEFA EVKNQEVTIK ALKEKIREYE
160 170 180 190 200
QTLKSQAETI ALEKEQKLQN DFAEKERKLQ ETQMSTTSKL EEAEHKLQTL
210 220 230 240 250
QTALEKTRTE LFDLKTKYDE ETTAKADEIE MIMTDLERAN QRAEVAQREA
260 270 280 290 300
ETLREQLSSA NHSLQLASQI QKAPDVAIEV LTRSSLEVEL AAKEREIAQL
310 320 330 340 350
VEDVQRLQAS LTKLRENSAS QISQLEQQLN AKNSTLKQLE EKLKGQADYE
360 370 380 390 400
EVKKELNTLK SMEFAPSEGA GTQDSTKPLE VLLLEKNRSL QSENATLRIS
410 420 430 440 450
NSDLSGSARR KGRDQPESRR PGPLPASPPP QLPRNTGEQV SNTNGTHHFS
460 470 480 490 500
PAGLSQDFFS SNLASPSLPL ASTGKFALNS LLQRQLMQSF YSKAMQEAGS
510 520 530 540 550
TSTIFSTGPY STNSISSPSP LQQSPDVNGM APSPSQSESA GSISEGEEID
560 570 580 590 600
TAEIARQVKE QLIKHNIGQR IFGHYVLGLS QGSVSEILAR PKPWNKLTVR
610 620 630 640 650
GKEPFHKMKQ FLSDEQNILA LRSIQGRQRE NPGQSLNRLF QEVPKRRNRS
660 670 680 690 700
EGNITTRIRA SETGSDEAIK SILEQAKREL QVQKTAEPVQ TSSTSSSGNS
710 720 730 740 750
DDAIRSILQQ ARREMEAQQA ALDPALKPAP LSQPDLTILT PKHLSASPMS
760 770 780 790 800
TVSTYPPLAI SLKKTPAAPE TSTAALPSAP ALKKEAQDVP TLDPPGSADA
810 820 830 840 850
AQGVLRPMKS ELVRGSTWKD PWWSPIQPER RNLTSSEETK ADETTASGKE
860 870 880 890 900
RAGSSQPRAE RSQLQGPSAS AEYWKEWPSA ESPYSQSSEL SLTGASRSET
910 920 930 940 950
PQNSPLPSSP IVPMAKPAKP SVPPLTPEQY EVYMYQEVDT IELTRQVKEK
960 970 980 990 1000
LAKNGICQRI FGEKVLGLSQ GSVSDMLSRP KPWSKLTQKG REPFIRMQLW
1010 1020 1030 1040 1050
LNGELGQGVL PVQGQQQGPV LHSVASLQDP LQQGCVSSES TPKTSASCSP
1060 1070 1080 1090 1100
APESPMSSSE SVKSLTELVQ QPCPAIETSK EGKPPEPSDP PASDSQPTTP
1110 1120 1130 1140 1150
LPLSGHSALS IQELVAMSPE LDTYGITKRV KEVLTDNNLG QRLFGETILG
1160 1170 1180 1190 1200
LTQGSVSDLL ARPKPWHKLS LKGREPFVRM QLWLNDPNNV EKLMDMKRME
1210 1220 1230 1240 1250
KKAYMKRRHS SVSDSQPCEP PSVGIDYSQG ASPQPQHQLK KPRVVLAPEE
1260 1270 1280 1290 1300
KEALKRAYQQ KPYPSPKTIE ELATQLNLKT STVINWFHNY RSRIRRELFI
1310 1320 1330 1340 1350
EEIQAGSQGQ AGASDSPSAR SSRAAPSSEG DSCDGVEATD AEEPGGNIVA
1360 1370 1380 1390 1400
TKSQGGLAEV AAAPADREEA TQPAEKAKAQ PLCSGTPGQD DGEDASRPRP
1410 1420 1430 1440 1450
LPEGLADAPA PVPSLAAPAA GEDAATSATA PATATEAPGA ARAGPAERSS
1460 1470 1480 1490 1500
ALPSTSAPAN APARRPSSLQ SLFGLPEAAG ARDNPVRKKK AANLNSIIHR
1510
LEKAASREEP IEWEF
Length:1,515
Mass (Da):165,596
Last modified:September 27, 2005 - v3
Checksum:i72BC6E8D8ECD78DE
GO
Isoform 2 (identifier: P53564-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     406-507: Missing.

Show »
Length:1,413
Mass (Da):154,697
Checksum:i5C7D6BE7CA4AD3BE
GO
Isoform 3 (identifier: P53564-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-10: MLCVAGAKLK → MAANVGSMFQYWKRFDLQQLQ
     630-651: Missing.

Note: No experimental confirmation available.

Show »
Length:1,504
Mass (Da):164,487
Checksum:i5B1EEBFC0FA74487
GO
Isoform 4 (identifier: P53564-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-183: Missing.

Show »
Length:1,332
Mass (Da):144,361
Checksum:i6A6305042CCADD61
GO
Isoform 6 (identifier: P53564-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1055: Missing.

Show »
Length:460
Mass (Da):49,021
Checksum:iB425EAF9639B9916
GO
Isoform 1 (identifier: P70403-1) [UniParc]FASTAAdd to Basket

Also known as: CASP

The sequence of this isoform can be found in the external entry P70403.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:678
Mass (Da):77,269
GO

Sequence cautioni

The sequence BAD90358.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti276 – 2761V → VEQ in AAK59986. (PubMed:11544187)Curated
Sequence conflicti649 – 6491R → G in CAA52922. (PubMed:7910552)Curated
Sequence conflicti649 – 6491R → G in AAD12485. (PubMed:9858552)Curated
Sequence conflicti1480 – 14801G → A in CAA52922. (PubMed:7910552)Curated
Sequence conflicti1485 – 14851P → L in AAD12485. (PubMed:9858552)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 10551055Missing in isoform 6. 1 PublicationVSP_017360Add
BLAST
Alternative sequencei1 – 183183Missing in isoform 4. 1 PublicationVSP_015748Add
BLAST
Alternative sequencei1 – 1010MLCVAGAKLK → MAANVGSMFQYWKRFDLQQL Q in isoform 3. 1 PublicationVSP_015749
Alternative sequencei406 – 507102Missing in isoform 2. 1 PublicationVSP_002311Add
BLAST
Alternative sequencei630 – 65122Missing in isoform 3. 1 PublicationVSP_015750Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75013 mRNA. Translation: CAA52922.1.
U46684 mRNA. Translation: AAB41146.1.
AY037807 mRNA. Translation: AAK59986.1.
AK220173 mRNA. Translation: BAD90358.1. Different initiation.
AF004225 mRNA. Translation: AAD12485.1.
U46683 mRNA. Translation: AAC52775.1.
CCDSiCCDS71684.1. [P53564-3]
PIRiI48314.
RefSeqiNP_001278162.1. NM_001291233.1.
NP_001278163.1. NM_001291234.1.
NP_034116.3. NM_009986.4.
UniGeneiMm.320317.

Genome annotation databases

GeneIDi13047.
KEGGimmu:13047.
UCSCiuc009aap.1. mouse. [P53564-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75013 mRNA. Translation: CAA52922.1 .
U46684 mRNA. Translation: AAB41146.1 .
AY037807 mRNA. Translation: AAK59986.1 .
AK220173 mRNA. Translation: BAD90358.1 . Different initiation.
AF004225 mRNA. Translation: AAD12485.1 .
U46683 mRNA. Translation: AAC52775.1 .
CCDSi CCDS71684.1. [P53564-3 ]
PIRi I48314.
RefSeqi NP_001278162.1. NM_001291233.1.
NP_001278163.1. NM_001291234.1.
NP_034116.3. NM_009986.4.
UniGenei Mm.320317.

3D structure databases

ProteinModelPortali P53564.
SMRi P53564. Positions 545-633, 929-1019, 1111-1199.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198981. 1 interaction.
IntActi P53564. 3 interactions.
MINTi MINT-1634604.

PTM databases

PhosphoSitei P53564.

Proteomic databases

MaxQBi P53564.
PRIDEi P53564.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13047.
KEGGi mmu:13047.
UCSCi uc009aap.1. mouse. [P53564-1 ]

Organism-specific databases

CTDi 1523.
MGIi MGI:88568. Cux1.
Rougei Search...

Phylogenomic databases

HOGENOMi HOG000143386.
HOVERGENi HBG051268.
InParanoidi P53564.
KOi K09313.
PhylomeDBi P53564.

Miscellaneous databases

ChiTaRSi Cux1. mouse.
NextBioi 282952.
SOURCEi Search...

Gene expression databases

CleanExi MM_CUX1.
Genevestigatori P53564.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 3 hits.
InterProi IPR003350. CUT_dom.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
[Graphical view ]
Pfami PF02376. CUT. 3 hits.
PF00046. Homeobox. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 3 hits.
PROSITEi PS51042. CUT. 3 hits.
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse homeodomain protein Phox2 regulates Ncam promoter activity in concert with Cux/CDP and is a putative determinant of neurotransmitter phenotype."
    Valarche I., Tissier-Seta J.-P., Hirsch M.R., Martinez S., Goridis C., Brunet J.-F.
    Development 119:881-896(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
    Strain: A/J and BALB/c.
    Tissue: Brain.
  2. "A unique variant of a homeobox gene related to Drosophila cut is expressed in mouse testis."
    Vanden Heuvel G.B., Quaggin S.E., Igarashi P.
    Biol. Reprod. 55:731-739(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Testis.
  3. "The transcriptional repressor CDP (Cutl1) is essential for epithelial cell differentiation of the lung and the hair follicle."
    Ellis T., Gambardella L., Horcher M., Tschanz S., Capol J., Bertram P., Jochum W., Barrandon Y., Busslinger M.
    Genes Dev. 15:2307-2319(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), FUNCTION.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Embryonic tail.
  5. "Cux/CDP homeoprotein is a component of NF-muNR and represses the immunoglobulin heavy chain intronic enhancer by antagonizing the bright transcription activator."
    Wang Z., Goldstein A., Zong R.-T., Lin D., Neufeld E.J., Scheuermann R.H., Tucker P.W.
    Mol. Cell. Biol. 19:284-295(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 121-1515 (ISOFORM 2), FUNCTION.
    Strain: C57BL/6N.
  6. "Expression of a cut-related homeobox gene in developing and polycystic mouse kidney."
    Vanden Heuvel G.B., Bodmer R., McConnell K.R., Nagami G.T., Igarashi P.
    Kidney Int. 50:453-461(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 762-1515 (ISOFORMS 2/3/4/5).
  7. "Homeoproteins CDP and SATB1 interact: potential for tissue-specific regulation."
    Liu J., Barnett A., Neufeld E.J., Dudley J.P.
    Mol. Cell. Biol. 19:4918-4926(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SATB1.
  8. "SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCRbeta enhancer (Ebeta)."
    Kaul-Ghanekar R., Jalota-Badhwar A., Pavithra L., Tucker P., Chattopadhyay S.
    Nucleic Acids Res. 32:4862-4875(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH BANP, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCUX1_MOUSE
AccessioniPrimary (citable) accession number: P53564
Secondary accession number(s): O08994
, P70301, Q571L6, Q91ZD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 27, 2005
Last modified: November 26, 2014
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Asn-1285 may participate in regulating DNA-binding activity by promoting homo- and heterodimerization.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3