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Protein

Biotin biosynthesis cytochrome P450

Gene

bioI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the C-C bond cleavage of fatty acid linked to acyl carrier protein (ACP) to generate pimelic acid for biotin biosynthesis. It has high affinity for long-chain fatty acids with the greatest affinity for myristic acid.2 Publications

Catalytic activityi

A long-chain acyl-[acyl-carrier protein] + 2 reduced flavodoxin + 3 O2 = pimeloyl-[acyl-carrier protein] + an n-alkanal + 2 oxidized flavodoxin + 3 H2O.1 Publication

Cofactori

heme1 PublicationNote: Binds 1 heme group covalently per subunit.1 Publication

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60Substrate1
Binding sitei307Substrate1
Metal bindingi345Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

  • biotin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU30190-MONOMER.
MetaCyc:BSU30190-MONOMER.
BRENDAi1.14.15.12. 658.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin biosynthesis cytochrome P450 (EC:1.14.15.12)
Gene namesi
Name:bioI
Synonyms:CYP107H
Ordered Locus Names:BSU30190
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000522602 – 395Biotin biosynthesis cytochrome P450Add BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi250 ↔ 2751 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP53554.

Interactioni

Protein-protein interaction databases

DIPiDIP-46307N.
STRINGi224308.Bsubs1_010100016446.

Structurei

Secondary structure

1395
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi11 – 14Combined sources4
Helixi16 – 26Combined sources11
Beta strandi28 – 34Combined sources7
Beta strandi37 – 42Combined sources6
Helixi45 – 53Combined sources9
Helixi71 – 78Combined sources8
Helixi81 – 83Combined sources3
Helixi88 – 97Combined sources10
Helixi98 – 100Combined sources3
Helixi102 – 106Combined sources5
Helixi109 – 121Combined sources13
Turni122 – 126Combined sources5
Beta strandi127 – 130Combined sources4
Helixi131 – 134Combined sources4
Helixi136 – 148Combined sources13
Helixi152 – 154Combined sources3
Helixi155 – 166Combined sources12
Helixi167 – 169Combined sources3
Helixi175 – 201Combined sources27
Helixi207 – 213Combined sources7
Helixi222 – 252Combined sources31
Helixi255 – 263Combined sources9
Helixi265 – 267Combined sources3
Helixi268 – 278Combined sources11
Beta strandi284 – 291Combined sources8
Beta strandi293 – 295Combined sources3
Beta strandi298 – 300Combined sources3
Beta strandi305 – 309Combined sources5
Helixi310 – 313Combined sources4
Turni317 – 319Combined sources3
Beta strandi320 – 322Combined sources3
Helixi341 – 343Combined sources3
Helixi348 – 365Combined sources18
Beta strandi382 – 384Combined sources3
Beta strandi391 – 393Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EJBX-ray2.00B/D/F/H2-395[»]
3EJDX-ray2.10B/D/F/H2-395[»]
3EJEX-ray2.10B/D/F/H2-395[»]
ProteinModelPortaliP53554.
SMRiP53554.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53554.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni89 – 93Heme binding5
Regioni169 – 173Substrate binding5
Regioni285 – 287Heme binding3
Regioni343 – 345Heme binding3

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiENOG4106A3M. Bacteria.
COG2124. LUCA.
HOGENOMiHOG000243678.
InParanoidiP53554.
KOiK16593.
OMAiPTQMTAR.
PhylomeDBiP53554.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIASSTASS EFLKNPYSFY DTLRAVHPIY KGSFLKYPGW YVTGYEETAA
60 70 80 90 100
ILKDARFKVR TPLPESSTKY QDLSHVQNQM MLFQNQPDHR RLRTLASGAF
110 120 130 140 150
TPRTTESYQP YIIETVHHLL DQVQGKKKME VISDFAFPLA SFVIANIIGV
160 170 180 190 200
PEEDREQLKE WAASLIQTID FTRSRKALTE GNIMAVQAMA YFKELIQKRK
210 220 230 240 250
RHPQQDMISM LLKGREKDKL TEEEAASTCI LLAIAGHETT VNLISNSVLC
260 270 280 290 300
LLQHPEQLLK LRENPDLIGT AVEECLRYES PTQMTARVAS EDIDICGVTI
310 320 330 340 350
RQGEQVYLLL GAANRDPSIF TNPDVFDITR SPNPHLSFGH GHHVCLGSSL
360 370 380 390
ARLEAQIAIN TLLQRMPSLN LADFEWRYRP LFGFRALEEL PVTFE
Length:395
Mass (Da):44,865
Last modified:October 1, 1996 - v1
Checksum:iE4AC3AF2637ACE1A
GO

Mass spectrometryi

Molecular mass is 44732 Da from positions 2 - 395. Determined by ESI. 1 Publication
Molecular mass is 45348 Da from positions 2 - 395. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51868 Genomic DNA. Translation: AAB17462.1.
AF008220 Genomic DNA. Translation: AAC00266.1.
AL009126 Genomic DNA. Translation: CAB14997.1.
PIRiG69594.
RefSeqiNP_390897.1. NC_000964.3.
WP_004398783.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14997; CAB14997; BSU30190.
GeneIDi935928.
KEGGibsu:BSU30190.
PATRICi18977940. VBIBacSub10457_3158.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51868 Genomic DNA. Translation: AAB17462.1.
AF008220 Genomic DNA. Translation: AAC00266.1.
AL009126 Genomic DNA. Translation: CAB14997.1.
PIRiG69594.
RefSeqiNP_390897.1. NC_000964.3.
WP_004398783.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EJBX-ray2.00B/D/F/H2-395[»]
3EJDX-ray2.10B/D/F/H2-395[»]
3EJEX-ray2.10B/D/F/H2-395[»]
ProteinModelPortaliP53554.
SMRiP53554.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46307N.
STRINGi224308.Bsubs1_010100016446.

Proteomic databases

PaxDbiP53554.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14997; CAB14997; BSU30190.
GeneIDi935928.
KEGGibsu:BSU30190.
PATRICi18977940. VBIBacSub10457_3158.

Phylogenomic databases

eggNOGiENOG4106A3M. Bacteria.
COG2124. LUCA.
HOGENOMiHOG000243678.
InParanoidiP53554.
KOiK16593.
OMAiPTQMTAR.
PhylomeDBiP53554.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciBSUB:BSU30190-MONOMER.
MetaCyc:BSU30190-MONOMER.
BRENDAi1.14.15.12. 658.

Miscellaneous databases

EvolutionaryTraceiP53554.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002397. Cyt_P450_B.
IPR017972. Cyt_P450_CS.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00359. BP450.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOI_BACSU
AccessioniPrimary (citable) accession number: P53554
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.