Reviewed, UniProtKB/Swiss-Prot P53553 (AK2_BACST)

Names and origin

Protein names

Recommended name:
    Aspartokinase 2
    EC=2.7.2.4
Alternative name(s):
    Aspartokinase II
    Aspartate kinase 2

Gene names

Name:

lysC

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Enzyme regulation	Lysine-sensitive By similarity.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.
Subunit structure	Tetramer consisting of 2 isoforms Alpha (catalytic) and 2 isoforms Beta (function not known).
Sequence similarities	Belongs to the aspartokinase family. Contains 2 ACT domains.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Coding sequence diversity	Alternative initiation
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW amino acid binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Alpha (identifier: P53553-1)

Also known as: Aspartokinase 2 subunit alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Beta (identifier: P53553-2)

Also known as: Aspartokinase 2 subunit beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-245: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Aspartokinase 2		PRO_0000002371
Regions
	Domain	262 – 335	74	ACT 1
	Domain	339 – 404	66	ACT 2
Natural variations
	Alternative sequence	1 – 245	245	Missing in isoform Beta.		VSP_018654

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha (Aspartokinase 2 subunit alpha) [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2A848E6A7037B172	FASTA	407	44,002
	10 20 30 40 50 60 MGIIVQKFGG TSVGSIERIQ HVANRVIEEV QKGNDVVVVV SAMGKTTDEL VNLAKQISNH 70 80 90 100 110 120 PSKREMDMLL STGEQVSIAL LAMSLHEKGY KAVSLTGWQA GITTEEMHGN ARIMNIDTTR 130 140 150 160 170 180 IRRCLDEGAI VIVAGFQGVT ETGEITTLGR GGSDTTAVAL AAALKAEKCD IYTDVTGVFT 190 200 210 220 230 240 TDPRYVKTAR KIKEISYDEM LELANLGAGV LHPRAVEFAK NYEVPLEVRS SMENERGTMV 250 260 270 280 290 300 KEEVSMEQHL IVRGIAFEDQ VTRVTVVGIE KYLQSVATIF TALANRGINV DIIIQNATNS 310 320 330 340 350 360 ETASVSFSIR TEDLPETLQV LQALEGADVH YESGLAKVSI VGSGMISNPG VAARVFEVLA 370 380 390 400 DQGIEIKMVS ISEIKISTVI DEKYMVSAVE ELHEAFGLAE EAAAVRS
	Isoform Beta (Aspartokinase 2 subunit beta). Checksum: 3F8D01A6B3591229	FASTA	162	17,435
	10 20 30 40 50 60 MEQHLIVRGI AFEDQVTRVT VVGIEKYLQS VATIFTALAN RGINVDIIIQ NATNSETASV 70 80 90 100 110 120 SFSIRTEDLP ETLQVLQALE GADVHYESGL AKVSIVGSGM ISNPGVAARV FEVLADQGIE 130 140 150 160 IKMVSISEIK ISTVIDEKYM VSAVEELHEA FGLAEEAAAV RS

References

[1]	"Sequence of the Bacillus stearothermophilus gene encoding aspartokinase II." Cantoni R., Labo M., de Rossi E., Riccardi G. Gene 169:135-136(1996) [PubMed: 8635739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NUB3621.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L46351 Genomic DNA. Translation: AAB06216.1.
PIR	JC4640.
3D structure databases
SMR	P53553. Positions 4-396.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.4. 266715.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005260. Asp_kin_monofn. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00656. asp_kin_monofn. 1 hit. TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK2_BACST

Accession

Primary (citable) accession number: P53553

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)