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Protein

Putative meiotic phospholipase SPO1

Gene

SPO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates spindle pole duplication in meiosis I, but not in mitosis. Required for meiosis I, meiosis II chromosome segregation and spore formation. Binds phosphatidylinositol (4)P mono- and polyphosphates.2 Publications

GO - Molecular functioni

  • phospholipase activity Source: SGD

GO - Biological processi

  • ascospore-type prospore membrane assembly Source: SGD
  • phospholipid catabolic process Source: InterPro
  • spindle pole body duplication Source: SGD
  • spore membrane bending pathway Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Meiosis, Sporulation

Enzyme and pathway databases

BioCyciYEAST:YNL012W-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative meiotic phospholipase SPO1 (EC:3.1.1.-)
Alternative name(s):
Sporulation-specific protein 1
Gene namesi
Name:SPO1
Ordered Locus Names:YNL012W
ORF Names:N2858
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL012W
SGDiS000004957 SPO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi103I → F in SPO1-1; defective in sporulation at 34 degrees Celsius. 1 Publication1
Mutagenesisi122S → A: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000012056918 – 631Putative meiotic phospholipase SPO1Add BLAST614

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi233N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi303N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi500N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi536N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi560N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi563N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi572N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP53541
PRIDEiP53541

Expressioni

Inductioni

Induced early in sporulation. Not detected during vegetative growth.1 Publication

Interactioni

Subunit structurei

Interacts with SPO23.1 Publication

Protein-protein interaction databases

BioGridi35813109 interactors.
DIPiDIP-1650N
IntActiP53541 15 interactors.
MINTiP53541
STRINGi4932.YNL012W

Structurei

3D structure databases

ProteinModelPortaliP53541
SMRiP53541
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 631PLA2cPROSITE-ProRule annotationAdd BLAST608

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 67Required for lipid-binding and function in meiosisAdd BLAST44

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000051940
HOGENOMiHOG000074554
InParanoidiP53541
KOiK13357
OMAiDDGQNIP
OrthoDBiEOG092C13BG

Family and domain databases

InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR002642 LysoPLipase_cat_dom
PfamiView protein in Pfam
PF01735 PLA2_B, 2 hits
SMARTiView protein in SMART
SM00022 PLAc, 1 hit
SUPFAMiSSF52151 SSF52151, 2 hits
PROSITEiView protein in PROSITE
PS51210 PLA2C, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53541-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKLLFVFSV LLTVVLATAP FQVQCPSSPL IREAKHELCP EETLYLKKKK
60 70 80 90 100
IKTKNKLIQF LKSLTEAKFS SKFYKRVLKD PPKIGIAISG GGYRSMLVGT
110 120 130 140 150
GFISQMNDYG LFEYSDYIAG LSGGSWILMD LVVQNFEVKS LLQEWDLEED
160 170 180 190 200
LLLGIPEFDI SEEEIVTNAK KEYNDNDLKM KKRQGGSLIT SSSNFYEQIE
210 220 230 240 250
EIMNSIEEIP EDYMITKRNL NPLARLKKIF FPNNTFTGTD AKIETFKKVL
260 270 280 290 300
DFYKSLHLKI KPKKMEGFQI SFTDYWGKAI VQRLKKNFDD DPNHSFSFSK
310 320 330 340 350
LVNSSKKFKE CSVPIPIFVA NCKNGLLSNV IFEFTPFEFG SWENILRLFV
360 370 380 390 400
KLPYLGSKIV SGKAEKCINN FDDLGFITAT SSSIFNNVLI FIWNLASQSS
410 420 430 440 450
REAMKALNMV MGIFGLGKEE IFSISKDSSR LETDYAVYQP NPFYLYPEKD
460 470 480 490 500
NVLTNKNHLY LVDGGEDGEN IPLRTLVIPE RELDVIFVLD SSSDIDNYPN
510 520 530 540 550
GSKLKRIFEK LDEENVHYQF PNNVKTFTHP IVIGCNATKR TGHDSFLPII
560 570 580 590 600
IYHANANHGN ASNTSTFKIT YNQSEVSSML PTGRGVFSND YDLYYKNCLG
610 620 630
CILTKRTMDR LPRKKKFSPF CLQCFKDYCY S
Length:631
Mass (Da):72,187
Last modified:September 5, 2006 - v2
Checksum:i2861BD03FB19C2D3
GO

Sequence cautioni

The sequence AAB38425 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAA95872 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L39372 Genomic DNA Translation: AAB38425.1 Sequence problems.
Z71288 Genomic DNA Translation: CAA95872.1 Sequence problems.
BK006947 Genomic DNA Translation: DAA10531.1
PIRiS62110
RefSeqiNP_014386.2, NM_001182851.1

Genome annotation databases

EnsemblFungiiYNL012W; YNL012W; YNL012W
GeneIDi855720
KEGGisce:YNL012W

Similar proteinsi

Entry informationi

Entry nameiSPO1_YEAST
AccessioniPrimary (citable) accession number: P53541
Secondary accession number(s): D6W1G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 5, 2006
Last modified: March 28, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome