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Protein

RNA polymerase II subunit A C-terminal domain phosphatase SSU72

Gene

SSU72

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. SSU72 is required for 3'-end formation of snoRNAs.
Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  • CTD phosphatase activity Source: SGD
  • phosphoprotein phosphatase activity Source: SGD
  • protein serine/threonine phosphatase activity Source: SGD
  • protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  • dephosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • mRNA 3'-end processing Source: SGD
  • mRNA polyadenylation Source: GO_Central
  • pre-mRNA cleavage required for polyadenylation Source: SGD
  • snoRNA transcription Source: SGD
  • termination of RNA polymerase II transcription Source: SGD
  • termination of RNA polymerase II transcription, exosome-dependent Source: SGD
  • termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD
  • transcriptional start site selection at RNA polymerase II promoter Source: SGD
  • transcription antitermination Source: SGD
  • transcription elongation from RNA polymerase II promoter Source: SGD
  • transcription initiation from RNA polymerase II promoter Source: SGD

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processmRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33225-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (EC:3.1.3.16)
Short name:
CTD phosphatase SSU72
Alternative name(s):
Suppressor of SUA7 protein 2
Gene namesi
Name:SSU72
Ordered Locus Names:YNL222W
ORF Names:N1279
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL222W
SGDiS000005166 SSU72

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000722301 – 206RNA polymerase II subunit A C-terminal domain phosphatase SSU72Add BLAST206

Proteomic databases

MaxQBiP53538
PaxDbiP53538
PRIDEiP53538

PTM databases

iPTMnetiP53538

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PTA1Q013296EBI-18134,EBI-14145

Protein-protein interaction databases

BioGridi35614, 230 interactors
DIPiDIP-6700N
IntActiP53538, 16 interactors
MINTiP53538
STRINGi4932.YNL222W

Structurei

3D structure databases

ProteinModelPortaliP53538
SMRiP53538
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SSU72 phosphatase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010165
HOGENOMiHOG000183445
InParanoidiP53538
KOiK15544
OMAiWQERWPN
OrthoDBiEOG092C4UKX

Family and domain databases

InterProiView protein in InterPro
IPR006811 RNA_pol_II_suA
PANTHERiPTHR20383 PTHR20383, 1 hit
PfamiView protein in Pfam
PF04722 Ssu72, 1 hit

Sequencei

Sequence statusi: Complete.

P53538-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSHRNSNLK FCTVCASNNN RSMESHKVLQ EAGYNVSSYG TGSAVRLPGL
60 70 80 90 100
SIDKPNVYSF GTPYNDIYND LLSQSADRYK SNGLLQMLDR NRRLKKAPEK
110 120 130 140 150
WQESTKVFDF VFTCEERCFD AVCEDLMNRG GKLNKIVHVI NVDIKDDDEN
160 170 180 190 200
AKIGSKAILE LADMLNDKIE QCEKDDIPFE DCIMDILTEW QSSHSQLPSL

YAPSYY
Length:206
Mass (Da):23,469
Last modified:October 1, 1996 - v1
Checksum:i1AE1B29B87C82FE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti199S → P in AAS56747 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20390 Genomic DNA Translation: AAA86497.1
Z71498 Genomic DNA Translation: CAA96125.1
AY558421 Genomic DNA Translation: AAS56747.1
BK006947 Genomic DNA Translation: DAA10334.1
PIRiS63180
RefSeqiNP_014177.1, NM_001183060.1

Genome annotation databases

EnsemblFungiiYNL222W; YNL222W; YNL222W
GeneIDi855499
KEGGisce:YNL222W

Similar proteinsi

Entry informationi

Entry nameiSSU72_YEAST
AccessioniPrimary (citable) accession number: P53538
Secondary accession number(s): D6W0W8, Q6Q569
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health