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P53538

- SSU72_YEAST

UniProt

P53538 - SSU72_YEAST

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Protein
RNA polymerase II subunit A C-terminal domain phosphatase SSU72
Gene
SSU72, YNL222W, N1279
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. SSU72 is required for 3'-end formation of snoRNAs.2 Publications
Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

GO - Molecular functioni

  1. CTD phosphatase activity Source: SGD
  2. phosphoprotein phosphatase activity Source: SGD
  3. protein serine/threonine phosphatase activity Source: SGD
  4. protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  1. dephosphorylation of RNA polymerase II C-terminal domain Source: SGD
  2. mRNA 3'-end processing Source: SGD
  3. mRNA cleavage Source: SGD
  4. peptidyl-tyrosine dephosphorylation Source: GOC
  5. snoRNA transcription Source: SGD
  6. termination of RNA polymerase II transcription Source: SGD
  7. termination of RNA polymerase II transcription, exosome-dependent Source: SGD
  8. termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD
  9. transcription antitermination Source: SGD
  10. transcription elongation from RNA polymerase II promoter Source: SGD
  11. transcription initiation from RNA polymerase II promoter Source: SGD
  12. transcriptional start site selection at RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

mRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-33225-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (EC:3.1.3.16)
Short name:
CTD phosphatase SSU72
Alternative name(s):
Suppressor of SUA7 protein 2
Gene namesi
Name:SSU72
Ordered Locus Names:YNL222W
ORF Names:N1279
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL222w.
SGDiS000005166. SSU72.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. mRNA cleavage and polyadenylation specificity factor complex Source: SGD
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206RNA polymerase II subunit A C-terminal domain phosphatase SSU72
PRO_0000072230Add
BLAST

Proteomic databases

MaxQBiP53538.
PaxDbiP53538.
PeptideAtlasiP53538.

Expressioni

Gene expression databases

GenevestigatoriP53538.

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.1 Publication

Protein-protein interaction databases

BioGridi35614. 134 interactions.
DIPiDIP-6700N.
IntActiP53538. 16 interactions.
MINTiMINT-432815.
STRINGi4932.YNL222W.

Structurei

3D structure databases

ProteinModelPortaliP53538.
SMRiP53538. Positions 9-191.

Family & Domainsi

Sequence similaritiesi

Belongs to the SSU72 phosphatase family.

Phylogenomic databases

eggNOGiCOG5211.
GeneTreeiENSGT00390000010165.
HOGENOMiHOG000183445.
KOiK15544.
OMAiMAKSNDI.
OrthoDBiEOG7GTTG0.

Family and domain databases

InterProiIPR006811. RNA_pol_II_suA.
[Graphical view]
PANTHERiPTHR20383. PTHR20383. 1 hit.
PfamiPF04722. Ssu72. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53538-1 [UniParc]FASTAAdd to Basket

« Hide

MPSHRNSNLK FCTVCASNNN RSMESHKVLQ EAGYNVSSYG TGSAVRLPGL    50
SIDKPNVYSF GTPYNDIYND LLSQSADRYK SNGLLQMLDR NRRLKKAPEK 100
WQESTKVFDF VFTCEERCFD AVCEDLMNRG GKLNKIVHVI NVDIKDDDEN 150
AKIGSKAILE LADMLNDKIE QCEKDDIPFE DCIMDILTEW QSSHSQLPSL 200
YAPSYY 206
Length:206
Mass (Da):23,469
Last modified:October 1, 1996 - v1
Checksum:i1AE1B29B87C82FE9
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti199 – 1991S → P in AAS56747. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20390 Genomic DNA. Translation: AAA86497.1.
Z71498 Genomic DNA. Translation: CAA96125.1.
AY558421 Genomic DNA. Translation: AAS56747.1.
BK006947 Genomic DNA. Translation: DAA10334.1.
PIRiS63180.
RefSeqiNP_014177.1. NM_001183060.1.

Genome annotation databases

EnsemblFungiiYNL222W; YNL222W; YNL222W.
GeneIDi855499.
KEGGisce:YNL222W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U20390 Genomic DNA. Translation: AAA86497.1 .
Z71498 Genomic DNA. Translation: CAA96125.1 .
AY558421 Genomic DNA. Translation: AAS56747.1 .
BK006947 Genomic DNA. Translation: DAA10334.1 .
PIRi S63180.
RefSeqi NP_014177.1. NM_001183060.1.

3D structure databases

ProteinModelPortali P53538.
SMRi P53538. Positions 9-191.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35614. 134 interactions.
DIPi DIP-6700N.
IntActi P53538. 16 interactions.
MINTi MINT-432815.
STRINGi 4932.YNL222W.

Proteomic databases

MaxQBi P53538.
PaxDbi P53538.
PeptideAtlasi P53538.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL222W ; YNL222W ; YNL222W .
GeneIDi 855499.
KEGGi sce:YNL222W.

Organism-specific databases

CYGDi YNL222w.
SGDi S000005166. SSU72.

Phylogenomic databases

eggNOGi COG5211.
GeneTreei ENSGT00390000010165.
HOGENOMi HOG000183445.
KOi K15544.
OMAi MAKSNDI.
OrthoDBi EOG7GTTG0.

Enzyme and pathway databases

BioCyci YEAST:G3O-33225-MONOMER.

Miscellaneous databases

NextBioi 979493.
PROi P53538.

Gene expression databases

Genevestigatori P53538.

Family and domain databases

InterProi IPR006811. RNA_pol_II_suA.
[Graphical view ]
PANTHERi PTHR20383. PTHR20383. 1 hit.
Pfami PF04722. Ssu72. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo."
    Sun Z.W., Hampsey M.
    Mol. Cell. Biol. 16:1557-1566(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast."
    Ganem C., Devaux F., Torchet C., Jacq C., Quevillon-Cheruel S., Labesse G., Facca C., Faye G.
    EMBO J. 22:1588-1598(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN SNORNA 3-END FORMATION.
  6. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
    Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
    J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CPF COMPLEX, COMPOSITION OF THE APT COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  7. Cited for: FUNCTION AS A PHOSPHATASE, FUNCTION IN SNORNA 3'-END FORMATION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSSU72_YEAST
AccessioniPrimary (citable) accession number: P53538
Secondary accession number(s): D6W0W8, Q6Q569
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

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