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P53538

- SSU72_YEAST

UniProt

P53538 - SSU72_YEAST

Protein

RNA polymerase II subunit A C-terminal domain phosphatase SSU72

Gene

SSU72

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. Component of the APT complex, which may be involved in polyadenylation-independent transcript 3'-end formation. SSU72 is required for 3'-end formation of snoRNAs.
    Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    GO - Molecular functioni

    1. CTD phosphatase activity Source: SGD
    2. phosphoprotein phosphatase activity Source: SGD
    3. protein serine/threonine phosphatase activity Source: SGD
    4. protein tyrosine phosphatase activity Source: SGD

    GO - Biological processi

    1. dephosphorylation of RNA polymerase II C-terminal domain Source: SGD
    2. mRNA 3'-end processing Source: SGD
    3. mRNA cleavage Source: SGD
    4. peptidyl-tyrosine dephosphorylation Source: GOC
    5. snoRNA transcription Source: SGD
    6. termination of RNA polymerase II transcription Source: SGD
    7. termination of RNA polymerase II transcription, exosome-dependent Source: SGD
    8. termination of RNA polymerase II transcription, poly(A)-coupled Source: SGD
    9. transcriptional start site selection at RNA polymerase II promoter Source: SGD
    10. transcription antitermination Source: SGD
    11. transcription elongation from RNA polymerase II promoter Source: SGD
    12. transcription initiation from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    mRNA processing

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33225-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA polymerase II subunit A C-terminal domain phosphatase SSU72 (EC:3.1.3.16)
    Short name:
    CTD phosphatase SSU72
    Alternative name(s):
    Suppressor of SUA7 protein 2
    Gene namesi
    Name:SSU72
    Ordered Locus Names:YNL222W
    ORF Names:N1279
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL222w.
    SGDiS000005166. SSU72.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. mRNA cleavage and polyadenylation specificity factor complex Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 206206RNA polymerase II subunit A C-terminal domain phosphatase SSU72PRO_0000072230Add
    BLAST

    Proteomic databases

    MaxQBiP53538.
    PaxDbiP53538.
    PeptideAtlasiP53538.

    Expressioni

    Gene expression databases

    GenevestigatoriP53538.

    Interactioni

    Subunit structurei

    Component of the cleavage and polyadenylation factor (CPF) complex, which is composed of PTI1, SYC1, SSU72, GLC7, MPE1, REF2, PFS2, PTA1, YSH1/BRR5, SWD2, CFT2/YDH1, YTH1, CFT1/YHH1, FIP1 and PAP1. Component of the APT complex, which is a subcomplex of CPF, and is composed of PTI1, SYC1, SSU72, GLC7, REF2, PTA1 and SWD2.1 Publication

    Protein-protein interaction databases

    BioGridi35614. 134 interactions.
    DIPiDIP-6700N.
    IntActiP53538. 16 interactions.
    MINTiMINT-432815.
    STRINGi4932.YNL222W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53538.
    SMRiP53538. Positions 9-191.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SSU72 phosphatase family.Curated

    Phylogenomic databases

    eggNOGiCOG5211.
    GeneTreeiENSGT00390000010165.
    HOGENOMiHOG000183445.
    KOiK15544.
    OMAiMAKSNDI.
    OrthoDBiEOG7GTTG0.

    Family and domain databases

    InterProiIPR006811. RNA_pol_II_suA.
    [Graphical view]
    PANTHERiPTHR20383. PTHR20383. 1 hit.
    PfamiPF04722. Ssu72. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSHRNSNLK FCTVCASNNN RSMESHKVLQ EAGYNVSSYG TGSAVRLPGL    50
    SIDKPNVYSF GTPYNDIYND LLSQSADRYK SNGLLQMLDR NRRLKKAPEK 100
    WQESTKVFDF VFTCEERCFD AVCEDLMNRG GKLNKIVHVI NVDIKDDDEN 150
    AKIGSKAILE LADMLNDKIE QCEKDDIPFE DCIMDILTEW QSSHSQLPSL 200
    YAPSYY 206
    Length:206
    Mass (Da):23,469
    Last modified:October 1, 1996 - v1
    Checksum:i1AE1B29B87C82FE9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti199 – 1991S → P in AAS56747. (PubMed:17322287)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20390 Genomic DNA. Translation: AAA86497.1.
    Z71498 Genomic DNA. Translation: CAA96125.1.
    AY558421 Genomic DNA. Translation: AAS56747.1.
    BK006947 Genomic DNA. Translation: DAA10334.1.
    PIRiS63180.
    RefSeqiNP_014177.1. NM_001183060.1.

    Genome annotation databases

    EnsemblFungiiYNL222W; YNL222W; YNL222W.
    GeneIDi855499.
    KEGGisce:YNL222W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U20390 Genomic DNA. Translation: AAA86497.1 .
    Z71498 Genomic DNA. Translation: CAA96125.1 .
    AY558421 Genomic DNA. Translation: AAS56747.1 .
    BK006947 Genomic DNA. Translation: DAA10334.1 .
    PIRi S63180.
    RefSeqi NP_014177.1. NM_001183060.1.

    3D structure databases

    ProteinModelPortali P53538.
    SMRi P53538. Positions 9-191.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35614. 134 interactions.
    DIPi DIP-6700N.
    IntActi P53538. 16 interactions.
    MINTi MINT-432815.
    STRINGi 4932.YNL222W.

    Proteomic databases

    MaxQBi P53538.
    PaxDbi P53538.
    PeptideAtlasi P53538.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL222W ; YNL222W ; YNL222W .
    GeneIDi 855499.
    KEGGi sce:YNL222W.

    Organism-specific databases

    CYGDi YNL222w.
    SGDi S000005166. SSU72.

    Phylogenomic databases

    eggNOGi COG5211.
    GeneTreei ENSGT00390000010165.
    HOGENOMi HOG000183445.
    KOi K15544.
    OMAi MAKSNDI.
    OrthoDBi EOG7GTTG0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33225-MONOMER.

    Miscellaneous databases

    NextBioi 979493.
    PROi P53538.

    Gene expression databases

    Genevestigatori P53538.

    Family and domain databases

    InterProi IPR006811. RNA_pol_II_suA.
    [Graphical view ]
    PANTHERi PTHR20383. PTHR20383. 1 hit.
    Pfami PF04722. Ssu72. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Synthetic enhancement of a TFIIB defect by a mutation in SSU72, an essential yeast gene encoding a novel protein that affects transcription start site selection in vivo."
      Sun Z.W., Hampsey M.
      Mol. Cell. Biol. 16:1557-1566(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Ssu72 is a phosphatase essential for transcription termination of snoRNAs and specific mRNAs in yeast."
      Ganem C., Devaux F., Torchet C., Jacq C., Quevillon-Cheruel S., Labesse G., Facca C., Faye G.
      EMBO J. 22:1588-1598(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN SNORNA 3-END FORMATION.
    6. "Organization and function of APT, a subcomplex of the yeast cleavage and polyadenylation factor involved in the formation of mRNA and small nucleolar RNA 3'-ends."
      Nedea E., He X., Kim M., Pootoolal J., Zhong G., Canadien V., Hughes T., Buratowski S., Moore C.L., Greenblatt J.
      J. Biol. Chem. 278:33000-33010(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE CPF COMPLEX, COMPOSITION OF THE APT COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    7. Cited for: FUNCTION AS A PHOSPHATASE, FUNCTION IN SNORNA 3'-END FORMATION.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSSU72_YEAST
    AccessioniPrimary (citable) accession number: P53538
    Secondary accession number(s): D6W0W8, Q6Q569
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3