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Protein

Elongation factor 4

Gene

lepA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Miscellaneous

The overproduction of this protein is lethal to M.leprae.

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 59GTPUniRule annotation6
Nucleotide bindingi174 – 177GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-671-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:ML0611
ORF Names:B1937_F3_81
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0611

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001763001 – 646Elongation factor 4Add BLAST646

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0611

Structurei

3D structure databases

ProteinModelPortaliP53530
SMRiP53530
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 227tr-type GAdd BLAST186

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S Bacteria
COG0481 LUCA
HOGENOMiHOG000020624
KOiK03596
OMAiALIFDSW
OrthoDBiPOG091H02HW

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.2570, 1 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SMARTiView protein in SMART
SM00838 EFG_C, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P53530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWSLRVDKP DTGESPFKHS LVTGAQEIPI SSFADKTFTA PAQIRNFCII
60 70 80 90 100
AHIDHGKSTL ADRMLQLTGV VDERSMRAQY LDRMDIERER GITIKAQNVR
110 120 130 140 150
LSWSVTAGGT TENYVLHLID TPGHVDFTYE VSRALEACEG AVLLVDAVQG
160 170 180 190 200
IEAQTLANLY LALERDLTII PVLNKIDLPA ADPDRYAAEI AHIIGYESGD
210 220 230 240 250
VLRVSGKTGE GVSDLLDEVV RRVPHPQGDP DAPTRAMIFD SVYDIYRGVV
260 270 280 290 300
TYVRVVDGKI SPRERIAMMS TGATYELLEV GIVSPEPKAS AGLGVGEVGY
310 320 330 340 350
LITGVKDVRQ SKVGDTVTTV RYGATEPLTG YREPKPMVYS GLYPVDSSDY
360 370 380 390 400
PSLRDALGKL QLNDAALTYE PETSVALGVG YRCGFLGLLH IDITRERLER
410 420 430 440 450
EFDLDLISTS PNVVYRVVTE DNTEIVVTNP SDWPEGKIRT VYEPVVKITI
460 470 480 490 500
IAPSEFIGTI MELCQSRRGE LGGMDYLSPE RVELRYIMPL GEIIFDFFDS
510 520 530 540 550
LKSRTRGYAS LDYEEAGEQE AQLVKVDILL QGEAVDAFSA IVHKDSASAY
560 570 580 590 600
GNKMTNKLKE LIPRQQFEVP VQAAIGSKVI ARENIRAIRK DVLSKCYGGD
610 620 630 640
ITRKRKLLEK QKEGKKRMKT IGRVEVPQEA FVAALSADAA GDKDKK
Length:646
Mass (Da):71,329
Last modified:October 1, 1996 - v1
Checksum:i256DC1EAE894C4A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00016 Genomic DNA Translation: AAA17177.1
AL583919 Genomic DNA Translation: CAC30119.1
PIRiS72609
RefSeqiNP_301513.1, NC_002677.1
WP_010907837.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC30119; CAC30119; CAC30119
GeneIDi910558
KEGGimle:ML0611
PATRICifig|272631.5.peg.1072

Similar proteinsi

Entry informationi

Entry nameiLEPA_MYCLE
AccessioniPrimary (citable) accession number: P53530
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health