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Protein

Elongation factor 4

Gene

lepA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi54 – 596GTPUniRule annotation
Nucleotide bindingi174 – 1774GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:ML0611
ORF Names:B1937_F3_81
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0611.

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 646646Elongation factor 4PRO_0000176300Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0611.

Structurei

3D structure databases

ProteinModelPortaliP53530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 227186tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
KOiK03596.
OMAiKPMVFCG.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53530-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWSLRVDKP DTGESPFKHS LVTGAQEIPI SSFADKTFTA PAQIRNFCII
60 70 80 90 100
AHIDHGKSTL ADRMLQLTGV VDERSMRAQY LDRMDIERER GITIKAQNVR
110 120 130 140 150
LSWSVTAGGT TENYVLHLID TPGHVDFTYE VSRALEACEG AVLLVDAVQG
160 170 180 190 200
IEAQTLANLY LALERDLTII PVLNKIDLPA ADPDRYAAEI AHIIGYESGD
210 220 230 240 250
VLRVSGKTGE GVSDLLDEVV RRVPHPQGDP DAPTRAMIFD SVYDIYRGVV
260 270 280 290 300
TYVRVVDGKI SPRERIAMMS TGATYELLEV GIVSPEPKAS AGLGVGEVGY
310 320 330 340 350
LITGVKDVRQ SKVGDTVTTV RYGATEPLTG YREPKPMVYS GLYPVDSSDY
360 370 380 390 400
PSLRDALGKL QLNDAALTYE PETSVALGVG YRCGFLGLLH IDITRERLER
410 420 430 440 450
EFDLDLISTS PNVVYRVVTE DNTEIVVTNP SDWPEGKIRT VYEPVVKITI
460 470 480 490 500
IAPSEFIGTI MELCQSRRGE LGGMDYLSPE RVELRYIMPL GEIIFDFFDS
510 520 530 540 550
LKSRTRGYAS LDYEEAGEQE AQLVKVDILL QGEAVDAFSA IVHKDSASAY
560 570 580 590 600
GNKMTNKLKE LIPRQQFEVP VQAAIGSKVI ARENIRAIRK DVLSKCYGGD
610 620 630 640
ITRKRKLLEK QKEGKKRMKT IGRVEVPQEA FVAALSADAA GDKDKK
Length:646
Mass (Da):71,329
Last modified:October 1, 1996 - v1
Checksum:i256DC1EAE894C4A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00016 Genomic DNA. Translation: AAA17177.1.
AL583919 Genomic DNA. Translation: CAC30119.1.
PIRiS72609.
RefSeqiNP_301513.1. NC_002677.1.
WP_010907837.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC30119; CAC30119; CAC30119.
GeneIDi910558.
KEGGimle:ML0611.
PATRICi18052250. VBIMycLep78757_1072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00016 Genomic DNA. Translation: AAA17177.1.
AL583919 Genomic DNA. Translation: CAC30119.1.
PIRiS72609.
RefSeqiNP_301513.1. NC_002677.1.
WP_010907837.1. NC_002677.1.

3D structure databases

ProteinModelPortaliP53530.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0611.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC30119; CAC30119; CAC30119.
GeneIDi910558.
KEGGimle:ML0611.
PATRICi18052250. VBIMycLep78757_1072.

Organism-specific databases

LepromaiML0611.

Phylogenomic databases

eggNOGiENOG4105C4S. Bacteria.
COG0481. LUCA.
HOGENOMiHOG000020624.
KOiK03596.
OMAiKPMVFCG.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR031157. G_TR_CS.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Smith D.R., Robison K.
    Submitted (MAR-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiLEPA_MYCLE
AccessioniPrimary (citable) accession number: P53530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The overproduction of this protein is lethal to M.leprae.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.