Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Actin-12

Gene

ACT12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the reproductive actins.

Miscellaneous

There are 8 actin genes in A.thaliana.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-2029482 Regulation of actin dynamics for phagocytic cup formation
R-ATH-5663213 RHO GTPases Activate WASPs and WAVEs

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-12
Gene namesi
Name:ACT12
Ordered Locus Names:At3g46520
ORF Names:F12A12.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G46520
TAIRilocus:2075160 AT3G46520

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000888962 – 377Actin-12Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53497
PRIDEiP53497

Expressioni

Tissue specificityi

Displays particular expression in the root cap and pericycle tissues associated with lateral root development. Little or no reproductive-gene expression is detected in vegetative organs, such as stems, leaves, sepals and petals.

Developmental stagei

Expressed primarily in pollen.

Gene expression databases

ExpressionAtlasiP53497 baseline and differential
GenevisibleiP53497 AT

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Protein-protein interaction databases

BioGridi912517 interactors.
IntActiP53497 12 interactors.
STRINGi3702.AT3G46520.1

Structurei

3D structure databases

ProteinModelPortaliP53497
SMRiP53497
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676 Eukaryota
COG5277 LUCA
HOGENOMiHOG000233339
InParanoidiP53497
KOiK10355
OMAiATLAMCE
OrthoDBiEOG09360BTG
PhylomeDBiP53497

Family and domain databases

InterProiView protein in InterPro
IPR004000 Actin
IPR020902 Actin/actin-like_CS
IPR004001 Actin_CS
PANTHERiPTHR11937 PTHR11937, 1 hit
PfamiView protein in Pfam
PF00022 Actin, 1 hit
PRINTSiPR00190 ACTIN
SMARTiView protein in SMART
SM00268 ACTIN, 1 hit
PROSITEiView protein in PROSITE
PS00406 ACTINS_1, 1 hit
PS00432 ACTINS_2, 1 hit
PS01132 ACTINS_ACT_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP
110 120 130 140 150
EEHPVLLTEA PLNPKANREK MTQIMFETFN TPAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDH LMKILTERGY
210 220 230 240 250
SFTTTAEREI VRDMKEKLSY IALDYEQELE TSKTSSSVEK SFELPDGQVI
260 270 280 290 300
TIGAERFRCP EVLFQPSMIG MENPGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGTTMFGG IGDRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIA KAEYDESGPS IVHRKCF
Length:377
Mass (Da):41,795
Last modified:October 1, 1996 - v1
Checksum:iBD1C7C523DBB167B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27982 Genomic DNA Translation: AAB39405.1
AL133314 Genomic DNA Translation: CAB62322.1
CP002686 Genomic DNA Translation: AEE78168.1
CP002686 Genomic DNA Translation: ANM65018.1
BT003847 mRNA Translation: AAO41897.1
BT005073 mRNA Translation: AAO50606.1
PIRiS68110
RefSeqiNP_001327017.1, NM_001339262.1
NP_190236.1, NM_114519.3
UniGeneiAt.35913

Genome annotation databases

EnsemblPlantsiAT3G46520.1; AT3G46520.1; AT3G46520
AT3G46520.2; AT3G46520.2; AT3G46520
GeneIDi823805
GrameneiAT3G46520.1; AT3G46520.1; AT3G46520
AT3G46520.2; AT3G46520.2; AT3G46520
KEGGiath:AT3G46520

Similar proteinsi

Entry informationi

Entry nameiACT12_ARATH
AccessioniPrimary (citable) accession number: P53497
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome