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Protein

Actin-12

Gene

ACT12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the reproductive actins.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-12
Gene namesi
Name:ACT12
Ordered Locus Names:At3g46520
ORF Names:F12A12.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G46520.

Subcellular locationi

GO - Cellular componenti

  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytoskeleton Source: UniProtKB-SubCell
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 377376Actin-12PRO_0000088896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP53497.
PRIDEiP53497.

Expressioni

Tissue specificityi

Displays particular expression in the root cap and pericycle tissues associated with lateral root development. Little or no reproductive-gene expression is detected in vegetative organs, such as stems, leaves, sepals and petals.

Developmental stagei

Expressed primarily in pollen.

Gene expression databases

GenevisibleiP53497. AT.

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Protein-protein interaction databases

BioGridi9125. 17 interactions.
IntActiP53497. 12 interactions.
STRINGi3702.AT3G46520.1.

Structurei

3D structure databases

ProteinModelPortaliP53497.
SMRiP53497. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
HOGENOMiHOG000233339.
InParanoidiP53497.
KOiK10355.
OMAiHTTTFNS.
OrthoDBiEOG09360BTG.
PhylomeDBiP53497.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP
110 120 130 140 150
EEHPVLLTEA PLNPKANREK MTQIMFETFN TPAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDH LMKILTERGY
210 220 230 240 250
SFTTTAEREI VRDMKEKLSY IALDYEQELE TSKTSSSVEK SFELPDGQVI
260 270 280 290 300
TIGAERFRCP EVLFQPSMIG MENPGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGTTMFGG IGDRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIA KAEYDESGPS IVHRKCF
Length:377
Mass (Da):41,795
Last modified:October 1, 1996 - v1
Checksum:iBD1C7C523DBB167B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27982 Genomic DNA. Translation: AAB39405.1.
AL133314 Genomic DNA. Translation: CAB62322.1.
CP002686 Genomic DNA. Translation: AEE78168.1.
BT003847 mRNA. Translation: AAO41897.1.
BT005073 mRNA. Translation: AAO50606.1.
PIRiS68110.
RefSeqiNP_190236.1. NM_114519.2.
UniGeneiAt.35913.

Genome annotation databases

EnsemblPlantsiAT3G46520.1; AT3G46520.1; AT3G46520.
GeneIDi823805.
GrameneiAT3G46520.1; AT3G46520.1; AT3G46520.
KEGGiath:AT3G46520.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27982 Genomic DNA. Translation: AAB39405.1.
AL133314 Genomic DNA. Translation: CAB62322.1.
CP002686 Genomic DNA. Translation: AEE78168.1.
BT003847 mRNA. Translation: AAO41897.1.
BT005073 mRNA. Translation: AAO50606.1.
PIRiS68110.
RefSeqiNP_190236.1. NM_114519.2.
UniGeneiAt.35913.

3D structure databases

ProteinModelPortaliP53497.
SMRiP53497. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9125. 17 interactions.
IntActiP53497. 12 interactions.
STRINGi3702.AT3G46520.1.

Proteomic databases

PaxDbiP53497.
PRIDEiP53497.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G46520.1; AT3G46520.1; AT3G46520.
GeneIDi823805.
GrameneiAT3G46520.1; AT3G46520.1; AT3G46520.
KEGGiath:AT3G46520.

Organism-specific databases

TAIRiAT3G46520.

Phylogenomic databases

eggNOGiKOG0676. Eukaryota.
COG5277. LUCA.
HOGENOMiHOG000233339.
InParanoidiP53497.
KOiK10355.
OMAiHTTTFNS.
OrthoDBiEOG09360BTG.
PhylomeDBiP53497.

Miscellaneous databases

PROiP53497.

Gene expression databases

GenevisibleiP53497. AT.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACT12_ARATH
AccessioniPrimary (citable) accession number: P53497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 8 actin genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.