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P53492 (ACT7_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-7
Alternative name(s):
Actin-2
Gene names
Name:ACT7
Ordered Locus Names:At5g09810
ORF Names:MYH9.2
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins which is involved in the regulation of hormone-induced plant cell proliferation and callus formation. Ref.8

Subunit structure

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Subcellular location

Cytoplasmcytoskeleton.

Tissue specificity

Constitutively expressed at high levels in young expanding vegetative tissues. Also strongly expressed in the hypocotyl and seed coat. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.

Induction

Differentially regulated in response to exogenous hormone treatment. In particular, auxin is a strong inducer. Ref.8

Miscellaneous

There are 8 actin genes in A.thaliana.

Sequence similarities

Belongs to the actin family.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   LigandATP-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell division

Inferred from mutant phenotype PubMed 19304937. Source: TAIR

response to auxin

Inferred from expression pattern Ref.8. Source: TAIR

response to light stimulus

Inferred from expression pattern Ref.2. Source: TAIR

response to wounding

Inferred from expression pattern Ref.2. Source: TAIR

root development

Inferred from mutant phenotype PubMed 12535345PubMed 19304937. Source: TAIR

root epidermal cell differentiation

Inferred from mutant phenotype PubMed 19304937. Source: TAIR

root hair elongation

Inferred from mutant phenotype PubMed 19304937. Source: TAIR

seed germination

Inferred from mutant phenotype PubMed 12535345. Source: TAIR

   Cellular_componentcell wall

Inferred from direct assay PubMed 16287169. Source: TAIR

chloroplast envelope

Inferred from direct assay PubMed 20061580. Source: TAIR

chloroplast stroma

Inferred from direct assay PubMed 20061580. Source: TAIR

cytoskeleton

Inferred from sequence or structural similarity Ref.2. Source: TAIR

cytosol

Inferred from direct assay PubMed 21166475. Source: TAIR

mitochondrion

Inferred from direct assay PubMed 14671022. Source: TAIR

nucleolus

Inferred from direct assay PubMed 15496452. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 17317660PubMed 17644812. Source: TAIR

plasmodesma

Inferred from direct assay PubMed 21533090. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural constituent of cytoskeleton

Inferred from sequence or structural similarity Ref.2. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Actin-7
PRO_0000088892

Sequences

Sequence LengthMass (Da)Tools
P53492 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0BED4053EDA9F916

FASTA37741,736
        10         20         30         40         50         60 
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA 

        70         80         90        100        110        120 
QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP EEHPVLLTEA PLNPKANREK 

       130        140        150        160        170        180 
MTQIMFETFN VPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL 

       190        200        210        220        230        240 
DLAGRDLTDS LMKILTERGY MFTTTAEREI VRDIKEKLAY VALDYEQELE TAKSSSSVEK 

       250        260        270        280        290        300 
NYELPDGQVI TIGAERFRCP EVLFQPSLIG MEAPGIHETT YNSIMKCDVD IRKDLYGNIV 

       310        320        330        340        350        360 
LSGGSTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIS 

       370 
KSEYDESGPS IVHRKCF 

« Hide

References

« Hide 'large scale' references
[1]"A cDNA sequence encoding a novel actin from Arabidopsis thaliana."
Hong Y., Chua N.
Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The Arabidopsis ACT7 actin gene is expressed in rapidly developing tissues and responds to several external stimuli."
McDowell J.M., An Y.-Q., Huang S., McKinney E.C., Meagher R.B.
Plant Physiol. 111:699-711(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: cv. Columbia.
[3]McKinney E.C., An Y.-Q.
Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Structure and evolution of the actin gene family in Arabidopsis thaliana."
McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.
Genetics 142:587-602(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, CHARACTERIZATION.
Strain: cv. Columbia.
[8]"One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation."
Kandasamy M.K., Gilliland L.U., McKinney E.C., Meagher R.B.
Plant Cell 13:1541-1554(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
Strain: cv. Columbia.
[9]"Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37281 mRNA. Translation: AAA80356.1.
U27811 Genomic DNA. Translation: AAB52506.1.
AB016893 Genomic DNA. Translation: BAB09402.1.
CP002688 Genomic DNA. Translation: AED91450.1.
AY062702 mRNA. Translation: AAL32780.1.
AY063980 mRNA. Translation: AAL36336.1.
AY096397 mRNA. Translation: AAM20037.1.
AY114679 mRNA. Translation: AAM47998.1.
AY120779 mRNA. Translation: AAM53337.1.
PIRS68107.
RefSeqNP_196543.1. NM_121018.3.
UniGeneAt.23346.
At.23605.
At.24396.
At.65835.

3D structure databases

ProteinModelPortalP53492.
SMRP53492. Positions 10-377.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid16119. 9 interactions.
IntActP53492. 10 interactions.
STRING3702.AT5G09810.1-P.

Proteomic databases

PRIDEP53492.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G09810.1; AT5G09810.1; AT5G09810.
GeneID830841.
KEGGath:AT5G09810.

Organism-specific databases

TAIRAT5G09810.

Phylogenomic databases

HOGENOMHOG000233339.
InParanoidP53492.
KOK10355.
OMAAMCEEED.
PhylomeDBP53492.
ProtClustDBCLSN2682469.

Gene expression databases

GenevestigatorP53492.

Family and domain databases

InterProIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERPTHR11937. PTHR11937. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
PRINTSPR00190. ACTIN.
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameACT7_ARATH
AccessionPrimary (citable) accession number: P53492
Secondary accession number(s): P53495
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names