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Protein

Actin-7

Gene

ACT7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Essential component of cell cytoskeleton; plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. This is considered as one of the vegetative actins which is involved in the regulation of hormone-induced plant cell proliferation and callus formation.1 Publication

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: TAIR

GO - Biological processi

  • cell division Source: TAIR
  • response to auxin Source: TAIR
  • response to light stimulus Source: TAIR
  • response to wounding Source: TAIR
  • root development Source: TAIR
  • root epidermal cell differentiation Source: TAIR
  • root hair elongation Source: TAIR
  • seed germination Source: TAIR
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-7
Alternative name(s):
Actin-2
Gene namesi
Name:ACT7
Ordered Locus Names:At5g09810
ORF Names:MYH9.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G09810.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytoskeleton Source: TAIR
  • cytosol Source: TAIR
  • mitochondrion Source: TAIR
  • nucleolus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 377377Actin-7PRO_0000088892Add
BLAST

Proteomic databases

PRIDEiP53492.

Expressioni

Tissue specificityi

Constitutively expressed at high levels in young expanding vegetative tissues. Also strongly expressed in the hypocotyl and seed coat. Little or no expression is detected in mature pollen sacs, ovules, embryos or seeds.

Inductioni

Differentially regulated in response to exogenous hormone treatment. In particular, auxin is a strong inducer.1 Publication

Gene expression databases

GenevestigatoriP53492.

Interactioni

Subunit structurei

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. The binding of profilin to monomeric G-actin cause the sequestration of actin into profilactin complexes, and prevents the polymerization.

Protein-protein interaction databases

BioGridi16119. 9 interactions.
IntActiP53492. 11 interactions.
STRINGi3702.AT5G09810.1-P.

Structurei

3D structure databases

ProteinModelPortaliP53492.
SMRiP53492. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family.Curated

Phylogenomic databases

HOGENOMiHOG000233339.
InParanoidiP53492.
KOiK10355.
OMAiEDAKHYM.
PhylomeDBiP53492.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG
60 70 80 90 100
QKDAYVGDEA QSKRGILTLK YPIEHGIVSN WDDMEKIWHH TFYNELRVAP
110 120 130 140 150
EEHPVLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT
160 170 180 190 200
TGIVLDSGDG VSHTVPIYEG YALPHAILRL DLAGRDLTDS LMKILTERGY
210 220 230 240 250
MFTTTAEREI VRDIKEKLAY VALDYEQELE TAKSSSSVEK NYELPDGQVI
260 270 280 290 300
TIGAERFRCP EVLFQPSLIG MEAPGIHETT YNSIMKCDVD IRKDLYGNIV
310 320 330 340 350
LSGGSTMFPG IADRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS
360 370
LSTFQQMWIS KSEYDESGPS IVHRKCF
Length:377
Mass (Da):41,736
Last modified:October 1, 1996 - v1
Checksum:i0BED4053EDA9F916
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37281 mRNA. Translation: AAA80356.1.
U27811 Genomic DNA. Translation: AAB52506.1.
AB016893 Genomic DNA. Translation: BAB09402.1.
CP002688 Genomic DNA. Translation: AED91450.1.
AY062702 mRNA. Translation: AAL32780.1.
AY063980 mRNA. Translation: AAL36336.1.
AY096397 mRNA. Translation: AAM20037.1.
AY114679 mRNA. Translation: AAM47998.1.
AY120779 mRNA. Translation: AAM53337.1.
PIRiS68107.
RefSeqiNP_196543.1. NM_121018.3.
UniGeneiAt.23346.
At.23605.
At.24396.
At.65835.

Genome annotation databases

EnsemblPlantsiAT5G09810.1; AT5G09810.1; AT5G09810.
GeneIDi830841.
KEGGiath:AT5G09810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37281 mRNA. Translation: AAA80356.1.
U27811 Genomic DNA. Translation: AAB52506.1.
AB016893 Genomic DNA. Translation: BAB09402.1.
CP002688 Genomic DNA. Translation: AED91450.1.
AY062702 mRNA. Translation: AAL32780.1.
AY063980 mRNA. Translation: AAL36336.1.
AY096397 mRNA. Translation: AAM20037.1.
AY114679 mRNA. Translation: AAM47998.1.
AY120779 mRNA. Translation: AAM53337.1.
PIRiS68107.
RefSeqiNP_196543.1. NM_121018.3.
UniGeneiAt.23346.
At.23605.
At.24396.
At.65835.

3D structure databases

ProteinModelPortaliP53492.
SMRiP53492. Positions 10-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16119. 9 interactions.
IntActiP53492. 11 interactions.
STRINGi3702.AT5G09810.1-P.

Proteomic databases

PRIDEiP53492.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G09810.1; AT5G09810.1; AT5G09810.
GeneIDi830841.
KEGGiath:AT5G09810.

Organism-specific databases

TAIRiAT5G09810.

Phylogenomic databases

HOGENOMiHOG000233339.
InParanoidiP53492.
KOiK10355.
OMAiEDAKHYM.
PhylomeDBiP53492.

Miscellaneous databases

PROiP53492.

Gene expression databases

GenevestigatoriP53492.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR004001. Actin_CS.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
PRINTSiPR00190. ACTIN.
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS00406. ACTINS_1. 1 hit.
PS00432. ACTINS_2. 1 hit.
PS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cDNA sequence encoding a novel actin from Arabidopsis thaliana."
    Hong Y., Chua N.
    Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The Arabidopsis ACT7 actin gene is expressed in rapidly developing tissues and responds to several external stimuli."
    McDowell J.M., An Y.-Q., Huang S., McKinney E.C., Meagher R.B.
    Plant Physiol. 111:699-711(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: cv. Columbia.
  3. McKinney E.C., An Y.-Q.
    Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Structure and evolution of the actin gene family in Arabidopsis thaliana."
    McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.
    Genetics 142:587-602(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, CHARACTERIZATION.
    Strain: cv. Columbia.
  8. "One plant actin isovariant, ACT7, is induced by auxin and required for normal callus formation."
    Kandasamy M.K., Gilliland L.U., McKinney E.C., Meagher R.B.
    Plant Cell 13:1541-1554(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
    Strain: cv. Columbia.
  9. "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis thaliana."
    Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E., Rathjen J.P., Peck S.C.
    J. Proteomics 72:439-451(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiACT7_ARATH
AccessioniPrimary (citable) accession number: P53492
Secondary accession number(s): P53495
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 29, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 8 actin genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.