P53447 (ALDOB_SPAAU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase B
EC=4.1.2.13

Alternative name(s):
Liver-type aldolase

Gene names

Name:

aldob

Organism

Sparus aurata (Gilthead sea bream)

Taxonomic identifier

8175 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Perciformes › Percoidei › Sparidae › Sparus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 364	363	Fructose-bisphosphate aldolase B		PRO_0000216946
Sites
	Active site	188	1	Proton acceptor By similarity
	Active site	230	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	364	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53447 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: EA7A5E75AF7D72FA	FASTA	364	39,645
	10 20 30 40 50 60 MTHQFPSLSP EQKKELSDIA QRIVAPGKGI LAADESTGTM GKRFQNINVE NIEENRRCFR 70 80 90 100 110 120 DILFSTDASI ANCVGGIIFF HETLYQKSSN GKLFPQVVKE KGIVVGIKVD KGTAPLMGTD 130 140 150 160 170 180 KETTTQGLDG LSERCAQYKK DGCDFAKWRC VLKISDGCPF ALAIAENANV LARYASICQM 190 200 210 220 230 240 NGLVPIVEPE ILPDGDHDLQ RCQYATEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHSC 250 260 270 280 290 300 PKKFTPQEVA MATVTALRRT VPASVPGICF LSGGQSEEEA SIHLNAINQV PLHRPWKLTF 310 320 330 340 350 360 SYGRALQASA LAAWQGKDAN KAATQQVFVT RAKINGLASK GEYKPSGSAD QASQQSLYTA SYVY

References

[1]	"Cloning and characterisation of a fish aldolase B gene." Llewellyn L., Ramsurn V.P., Sweeney G.E., Wigham T., Santos C.R., Power D.M. Biochim. Biophys. Acta 1263:75-78(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82278 mRNA. Translation: CAA57729.1.
PIR	S48810. S57270.
3D structure databases
ProteinModelPortal	P53447.
SMR	P53447. Positions 3-364.
ModBase	Search...
Proteomic databases
PRIDE	P53447.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG002386.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDOB_SPAAU

Accession

Primary (citable) accession number: P53447

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program