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P53446 (ALF2_LAMJA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase, non-muscle type

EC=4.1.2.13
OrganismLampetra japonica (Japanese lamprey) (Entosphenus japonicus)
Taxonomic identifier980415 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataHyperoartiaPetromyzontiformesPetromyzontidaeLethenteron

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subunit structure

Homotetramer By similarity.

Tissue specificity

Expressed mainly in the liver and also in brain and other tissues, except for the heart muscle. Ref.1

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandSchiff base
   Molecular functionLyase
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 364364Fructose-bisphosphate aldolase, non-muscle type
PRO_0000216955

Sites

Active site2301Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site561Substrate By similarity
Binding site1471Substrate By similarity
Site3641Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

Sequences

Sequence LengthMass (Da)Tools
P53446 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 1F495DB6AAA1A7E5

FASTA36438,952
        10         20         30         40         50         60 
MAALYPALTP EQKKELAEIA QRIVSNGKGI LAADESTGTM GKRLSAIKVE NVDENRRVYR 

        70         80         90        100        110        120 
QLLFSSDPSV TKAIGGVIFY EETLYQKTDD GTPFVKLIKD RGIVVGIKVD KGTVPLAGTD 

       130        140        150        160        170        180 
GESTTQGLDG LAERCARYKK DGADFAKWRC VLKISKNTPS ALAIAENANV LARYASICQQ 

       190        200        210        220        230        240 
NGLVPIVEPE ILPDGDHDLK TCQYVTEKVL AATYKALSDH HVYLEGSLLK PNMVTAGQAS 

       250        260        270        280        290        300 
KIRCSPQEVA MATVTALRRT VPSAVPGITF LSGGQSEEDA SLNLNAINQL PLERPWALSF 

       310        320        330        340        350        360 
SYGRALQASV LKAWAGAPAN IPAAKKEFEK RAAINGLAAQ GKYVPAGSSG SAASESLFIA 


NHNY 

« Hide

References

[1]"Structures of cDNAs encoding the muscle-type and non-muscle-type isozymes of lamprey fructose bisphosphate aldolases and the evolution of aldolase genes."
Zhang R., Yatsuki H., Kusakabe T., Iwabe N., Miyata T., Imai T., Yoshida M., Hori K.
J. Biochem. 117:545-553(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D38619 mRNA. Translation: BAA07607.1.

3D structure databases

ProteinModelPortalP53446.
SMRP53446. Positions 5-364.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP53446.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG002386.

Enzyme and pathway databases

SABIO-RKP53446.
UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALF2_LAMJA
AccessionPrimary (citable) accession number: P53446
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 16, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways