P53445 (ALF1_LAMJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Fructose-bisphosphate aldolase, muscle type EC=4.1.2.13
Organism	Lampetra japonica (Japanese lamprey) (Entosphenus japonicus)
Taxonomic identifier	980415 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Hyperoartia › Petromyzontiformes › Petromyzontidae › Lethenteron

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer By similarity.
Tissue specificity	Expressed mainly in the skeletal muscle, heart muscle, brain, and some other tissues, but probably not in liver. Ref.1
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Fructose-bisphosphate aldolase, muscle type		PRO_0000216954
Sites
	Active site	230	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	363	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

Sequences

	Sequence		Length	Mass (Da)
	P53445 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 15BC9A40A7FB9EFC	FASTA	363	39,239
	10 20 30 40 50 60 MSPHFPALTP DQKKELADIA QRIVASGKGI LAADESVGTM GKRLTQIGLE NTDEHRRFYR 70 80 90 100 110 120 QLLFTTDPSI KEHIGGIIFF HETMYQKTDG GVPFVKLVKD NGILVGIKVD KGVVPLAGTN 130 140 150 160 170 180 GEGTTQGLDG LAERCAQYKK DGADFAKWRC VLKISPNTPS RLSIVENANV LARYATICQQ 190 200 210 220 230 240 NGLVPIVEPE ILPDGDHDLK TCQYITEKVL AATYKALSDH HVYLEGTLLK PNMVTVGTAA 250 260 270 280 290 300 PASTRPEQVA MATLTALRRT VPPAVPGITF LSGGQSEEDA SIHLNAINKL HLIKPWALTF 310 320 330 340 350 360 SYGRALQASV LKAWGGKKEN LKAAQDELMR RAKINGQASK GEYKPTGTGA AAGESLFVAN HAY

References

[1]

"Structures of cDNAs encoding the muscle-type and non-muscle-type isozymes of lamprey fructose bisphosphate aldolases and the evolution of aldolase genes." Zhang R., Yatsuki H., Kusakabe T., Iwabe N., Miyata T., Imai T., Yoshida M., Hori K. J. Biochem. 117:545-553(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38620 mRNA. Translation: BAA07608.1.
3D structure databases
ProteinModelPortal	P53445.
SMR	P53445. Positions 4-363.
ModBase	Search...
Proteomic databases
PRIDE	P53445.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG002386.
Enzyme and pathway databases
SABIO-RK	P53445.
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_LAMJA

Accession

Primary (citable) accession number: P53445

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program