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P53444

- ALF_NEUCR

UniProt

P53444 - ALF_NEUCR

Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 Mar 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Cofactori

    Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei63 – 631Glyceraldehyde 3-phosphateBy similarity
    Active sitei112 – 1121Proton donorBy similarity
    Metal bindingi113 – 1131Zinc 1; catalyticBy similarity
    Metal bindingi147 – 1471Zinc 2By similarity
    Metal bindingi177 – 1771Zinc 2By similarity
    Metal bindingi229 – 2291Zinc 1; catalyticBy similarity
    Binding sitei230 – 2301Dihydroxyacetone phosphate; via amide nitrogenBy similarity
    Metal bindingi268 – 2681Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase (EC:4.1.2.13)
    Short name:
    FBP aldolase
    Short name:
    FBPA
    Alternative name(s):
    Fructose-1,6-bisphosphate aldolase
    Gene namesi
    Name:fba
    ORF Names:NCU07807
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 362362Fructose-bisphosphate aldolasePRO_0000178759Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi5141.NCU07807.1.

    Structurei

    3D structure databases

    ProteinModelPortaliP53444.
    SMRiP53444. Positions 3-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni269 – 2713Dihydroxyacetone phosphate bindingBy similarity
    Regioni290 – 2934Dihydroxyacetone phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0191.
    HOGENOMiHOG000227794.
    KOiK01624.
    OMAiHNSLDFV.
    OrthoDBiEOG7HTHSN.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view]
    PfamiPF01116. F_bP_aldolase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsiTIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53444-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGIFDELNLP AGVLYGDDVL KLFQYAREKQ FAIPACNVTS SSTAVAALEA    50
    ARDQKAPIIL QTSQGGAAFF AGKGIKDSAE KREASVAGAI AAAHYIRSIA 100
    PIYGIPVVLH TDHCAKKLLP WLDGMLEEDE KFFKANGVPL FSSHMIDLSE 150
    EPVEENISTC VKYLKRMAPM KQWLEMEIGI TGGEEDGVDN SEVDNASLYT 200
    QPEDIWQIEE AFRPISPYFS IAAGFGNVHG VYAPGNVKLH PELLGKHQAY 250
    VSEKLGGKDK KPVFFVFHGG SGSSKEEYRE AISNGVVKVN VDTDLQWSYL 300
    VGIRDYILNN IDYLRSQVGN PEGPNKPNKK KYDPRVWIRE GEKTMKARVE 350
    EALKDFNAAG TV 362
    Length:362
    Mass (Da):39,889
    Last modified:March 1, 2004 - v2
    Checksum:iC402186EEEBD9368
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 1515MGIFD…AGVLY → MRCPIPEHKQNLTFSLPL in AAB00930. 1 PublicationCuratedAdd
    BLAST
    Sequence conflicti58 – 592II → SS in AAB00930. 1 PublicationCurated
    Sequence conflicti66 – 661G → C in AAB00930. 1 PublicationCurated
    Sequence conflicti82 – 843REA → ARD in AAB00930. 1 PublicationCurated
    Sequence conflicti109 – 1091L → RI in AAB00930. 1 PublicationCurated
    Sequence conflicti120 – 1267PWLDGML → FGDGIP in AAB00930. 1 PublicationCurated
    Sequence conflicti211 – 2122AF → DS in AAB00930. 1 PublicationCurated
    Sequence conflicti222 – 2221A → G in AAB00930. 1 PublicationCurated
    Sequence conflicti242 – 2421E → D in AAB00930. 1 PublicationCurated
    Sequence conflicti247 – 2471H → Q in AAB00930. 1 PublicationCurated
    Sequence conflicti252 – 2521S → P in AAB00930. 1 PublicationCurated
    Sequence conflicti263 – 2631V → F in AAB00930. 1 PublicationCurated
    Sequence conflicti352 – 3609ALKDFNAAG → GPQGPSNTTV in AAB00930. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42380 Genomic DNA. Translation: AAB00930.1.
    CM002240 Genomic DNA. Translation: ESA42348.1.
    CM002240 Genomic DNA. Translation: ESA42349.1.
    PIRiT47260.
    RefSeqiXP_001728093.1. XM_001728041.1.
    XP_958393.1. XM_953300.2.
    UniGeneiNcr.25490.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000008000; EFNCRP00000007989; EFNCRG00000007988.
    EFNCRT00000008001; EFNCRP00000007990; EFNCRG00000007988.
    GeneIDi3874540.
    KEGGincr:NCU07807.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42380 Genomic DNA. Translation: AAB00930.1 .
    CM002240 Genomic DNA. Translation: ESA42348.1 .
    CM002240 Genomic DNA. Translation: ESA42349.1 .
    PIRi T47260.
    RefSeqi XP_001728093.1. XM_001728041.1.
    XP_958393.1. XM_953300.2.
    UniGenei Ncr.25490.

    3D structure databases

    ProteinModelPortali P53444.
    SMRi P53444. Positions 3-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5141.NCU07807.1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000008000 ; EFNCRP00000007989 ; EFNCRG00000007988 .
    EFNCRT00000008001 ; EFNCRP00000007990 ; EFNCRG00000007988 .
    GeneIDi 3874540.
    KEGGi ncr:NCU07807.

    Phylogenomic databases

    eggNOGi COG0191.
    HOGENOMi HOG000227794.
    KOi K01624.
    OMAi HNSLDFV.
    OrthoDBi EOG7HTHSN.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR006411. Fruct_bisP_bact.
    IPR000771. Ketose_bisP_aldolase_II.
    [Graphical view ]
    Pfami PF01116. F_bP_aldolase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
    TIGRFAMsi TIGR00167. cbbA. 1 hit.
    TIGR01520. FruBisAldo_II_A. 1 hit.
    PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
    PS00806. ALDOLASE_CLASS_II_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA sequence of fructose-1,6-bisphosphate aldolase in N. crassa."
      Yamashita R., Stuart W.D.
      Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 74A.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

    Entry informationi

    Entry nameiALF_NEUCR
    AccessioniPrimary (citable) accession number: P53444
    Secondary accession number(s): A7UX41, Q7S1C1, V5IKX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: March 1, 2004
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3