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P53444

- ALF_NEUCR

UniProt

P53444 - ALF_NEUCR

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Protein
Fructose-bisphosphate aldolase
Gene
fba, NCU07807
Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631Glyceraldehyde 3-phosphate By similarity
Active sitei112 – 1121Proton donor By similarity
Metal bindingi113 – 1131Zinc 1; catalytic By similarity
Metal bindingi147 – 1471Zinc 2 By similarity
Metal bindingi177 – 1771Zinc 2 By similarity
Metal bindingi229 – 2291Zinc 1; catalytic By similarity
Binding sitei230 – 2301Dihydroxyacetone phosphate; via amide nitrogen By similarity
Metal bindingi268 – 2681Zinc 1; catalytic By similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
ORF Names:NCU07807
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 2, Linkage Group V

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Fructose-bisphosphate aldolase
PRO_0000178759Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

STRINGi5141.NCU07807.1.

Structurei

3D structure databases

ProteinModelPortaliP53444.
SMRiP53444. Positions 3-362.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni269 – 2713Dihydroxyacetone phosphate binding By similarity
Regioni290 – 2934Dihydroxyacetone phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227794.
KOiK01624.
OMAiHNSLDFV.
OrthoDBiEOG7HTHSN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53444-1 [UniParc]FASTAAdd to Basket

« Hide

MGIFDELNLP AGVLYGDDVL KLFQYAREKQ FAIPACNVTS SSTAVAALEA    50
ARDQKAPIIL QTSQGGAAFF AGKGIKDSAE KREASVAGAI AAAHYIRSIA 100
PIYGIPVVLH TDHCAKKLLP WLDGMLEEDE KFFKANGVPL FSSHMIDLSE 150
EPVEENISTC VKYLKRMAPM KQWLEMEIGI TGGEEDGVDN SEVDNASLYT 200
QPEDIWQIEE AFRPISPYFS IAAGFGNVHG VYAPGNVKLH PELLGKHQAY 250
VSEKLGGKDK KPVFFVFHGG SGSSKEEYRE AISNGVVKVN VDTDLQWSYL 300
VGIRDYILNN IDYLRSQVGN PEGPNKPNKK KYDPRVWIRE GEKTMKARVE 350
EALKDFNAAG TV 362
Length:362
Mass (Da):39,889
Last modified:March 1, 2004 - v2
Checksum:iC402186EEEBD9368
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1515MGIFD…AGVLY → MRCPIPEHKQNLTFSLPL in AAB00930. 1 Publication
Add
BLAST
Sequence conflicti58 – 592II → SS in AAB00930. 1 Publication
Sequence conflicti66 – 661G → C in AAB00930. 1 Publication
Sequence conflicti82 – 843REA → ARD in AAB00930. 1 Publication
Sequence conflicti109 – 1091L → RI in AAB00930. 1 Publication
Sequence conflicti120 – 1267PWLDGML → FGDGIP in AAB00930. 1 Publication
Sequence conflicti211 – 2122AF → DS in AAB00930. 1 Publication
Sequence conflicti222 – 2221A → G in AAB00930. 1 Publication
Sequence conflicti242 – 2421E → D in AAB00930. 1 Publication
Sequence conflicti247 – 2471H → Q in AAB00930. 1 Publication
Sequence conflicti252 – 2521S → P in AAB00930. 1 Publication
Sequence conflicti263 – 2631V → F in AAB00930. 1 Publication
Sequence conflicti352 – 3609ALKDFNAAG → GPQGPSNTTV in AAB00930. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42380 Genomic DNA. Translation: AAB00930.1.
CM002240 Genomic DNA. Translation: ESA42348.1.
CM002240 Genomic DNA. Translation: ESA42349.1.
PIRiT47260.
RefSeqiXP_001728093.1. XM_001728041.1.
XP_958393.1. XM_953300.2.
UniGeneiNcr.25490.

Genome annotation databases

EnsemblFungiiEFNCRT00000008000; EFNCRP00000007989; EFNCRG00000007988.
EFNCRT00000008001; EFNCRP00000007990; EFNCRG00000007988.
GeneIDi3874540.
KEGGincr:NCU07807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42380 Genomic DNA. Translation: AAB00930.1 .
CM002240 Genomic DNA. Translation: ESA42348.1 .
CM002240 Genomic DNA. Translation: ESA42349.1 .
PIRi T47260.
RefSeqi XP_001728093.1. XM_001728041.1.
XP_958393.1. XM_953300.2.
UniGenei Ncr.25490.

3D structure databases

ProteinModelPortali P53444.
SMRi P53444. Positions 3-362.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5141.NCU07807.1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000008000 ; EFNCRP00000007989 ; EFNCRG00000007988 .
EFNCRT00000008001 ; EFNCRP00000007990 ; EFNCRG00000007988 .
GeneIDi 3874540.
KEGGi ncr:NCU07807.

Phylogenomic databases

eggNOGi COG0191.
HOGENOMi HOG000227794.
KOi K01624.
OMAi HNSLDFV.
OrthoDBi EOG7HTHSN.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA sequence of fructose-1,6-bisphosphate aldolase in N. crassa."
    Yamashita R., Stuart W.D.
    Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 74A.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Entry informationi

Entry nameiALF_NEUCR
AccessioniPrimary (citable) accession number: P53444
Secondary accession number(s): A7UX41, Q7S1C1, V5IKX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 1, 2004
Last modified: June 11, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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