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Protein

Phosphatidate cytidylyltransferase

Gene

cdgs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Provides CDP-diacylglycerol, an important precursor for the synthesis of phosphatidylinositol (PtdIns).By similarity

Catalytic activityi

CTP + phosphatidate = diphosphate + CDP-diacylglycerol.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable glycerol-3-phosphate acyltransferase, mitochondrial (acl-6)
  2. Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase acl-2 (acl-2), Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase acl-1 (acl-1), Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase acl-12 (acl-12)
  3. Phosphatidate cytidylyltransferase (cdgs-1), Phosphatidate cytidylyltransferase, mitochondrial (Y71F9B.2)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-CEL-1483148 Synthesis of PG
R-CEL-1483226 Synthesis of PI
UniPathwayiUPA00557; UER00614

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate cytidylyltransferase (EC:2.7.7.41)
Alternative name(s):
CDP-DAG synthase
CDP-DG synthase
CDP-diacylglycerol synthase
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
Gene namesi
Name:cdgs-1
ORF Names:C33H5.18
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC33H5.18a ; CE04161 ; WBGene00016384 ; cdgs-1
C33H5.18b ; CE26907 ; WBGene00016384 ; cdgs-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei95 – 117HelicalSequence analysisAdd BLAST23
Transmembranei121 – 143HelicalSequence analysisAdd BLAST23
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Transmembranei367 – 387HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced levels of phosphatidylinositol. No effect on two-cell stage nuclear morphology. Significant rescue of the nuclear morphology defects resulting from inhibition of cnep-1 or partial inhibition of lpin-1 but does not rescue the nuclear envelope disassembly defects observed following depletion of npp-12.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000907191 – 465Phosphatidate cytidylyltransferaseAdd BLAST465

Proteomic databases

EPDiP53439
PaxDbiP53439
PeptideAtlasiP53439
PRIDEiP53439

PTM databases

iPTMnetiP53439

Expressioni

Gene expression databases

BgeeiWBGene00016384

Interactioni

Protein-protein interaction databases

IntActiP53439, 1 interactor
MINTiP53439
STRINGi6239.C33H5.18b

Structurei

3D structure databases

ProteinModelPortaliP53439
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1440 Eukaryota
COG0575 LUCA
GeneTreeiENSGT00390000016175
HOGENOMiHOG000209582
InParanoidiP53439
KOiK00981
OMAiVVQAHFI
OrthoDBiEOG091G06CA
PhylomeDBiP53439

Family and domain databases

InterProiView protein in InterPro
IPR000374 PC_trans
IPR016720 PC_Trfase_euk
PIRSFiPIRSF018269 PC_trans_euk, 1 hit
PROSITEiView protein in PROSITE
PS01315 CDS, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P53439-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDQPPAENA DVRQRRAPES PVTERLRAPA RDDARPTSDE SDMEGILQDE
60 70 80 90 100
DSDAGSKNKE EKLERLTQAI PQDKGSLGVF ADSMLEALPP RWRNWVVRGL
110 120 130 140 150
FSIIMISTFT FIVTRGATWL MFLVFLIQFK CFQEIISIGL AVYRLYDFPW
160 170 180 190 200
FRALSWYFLL TSNYFFFGES LIDYWGIVLK KDNFLHFLVA YHRLVSFALY
210 220 230 240 250
CIGFVSFVLS LRKGYYMRQF SLFAWTHLTL LLIVSQSFFI IQNIFQGLIW
260 270 280 290 300
FLAPVAMIIC CDIMSYMFGF FWGKTPLIKL SPKKTWEGFI GGAFSTVVFG
310 320 330 340 350
ILLSLALYNR PFFVCPVQHY QTDSSNCTIP LAFQLQDYPV PRPFSFVYKI
360 370 380 390 400
LRKEPIIQLC PFVFHSIALS LFASILGPFG GFFASGFKRA FKIKDFGDVI
410 420 430 440 450
PGHGGLMDRF DCQLLMGTFV MVYIHSFIRV PDASKLLKQI MTLEPQDQLN
460
IFNLLQSELS KTGLI
Note: No experimental confirmation available.
Length:465
Mass (Da):53,460
Last modified:September 3, 2014 - v2
Checksum:iD02E7C5F17E9D3DD
GO
Isoform a (identifier: P53439-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-62: SDAGSKNKEEK → R

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):52,442
Checksum:iECD8048CA0A28649
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05541752 – 62SDAGSKNKEEK → R in isoform a. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080768 Genomic DNA Translation: CCD66581.1
FO080768 Genomic DNA Translation: CCD66582.1
PIRiT34155
RefSeqiNP_501297.1, NM_068896.3 [P53439-2]
NP_501298.1, NM_068897.3 [P53439-1]
UniGeneiCel.17593

Genome annotation databases

EnsemblMetazoaiC33H5.18b.1; C33H5.18b.1; WBGene00016384 [P53439-1]
C33H5.18b.2; C33H5.18b.2; WBGene00016384 [P53439-1]
GeneIDi3565046
KEGGicel:CELE_C33H5.18
UCSCiC33H5.18b c. elegans [P53439-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCDSA_CAEEL
AccessioniPrimary (citable) accession number: P53439
Secondary accession number(s): G8JY50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 3, 2014
Last modified: March 28, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health