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Protein

Phosphatidate cytidylyltransferase

Gene

cdgs-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Provides CDP-diacylglycerol, an important precursor for the synthesis of phosphatidylinositol (PtdIns).By similarity

Catalytic activityi

CTP + phosphatidate = diphosphate + CDP-diacylglycerol.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Probable glycerol-3-phosphate acyltransferase, mitochondrial (acl-6)
  2. Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase acl-2 (acl-2), Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase acl-1 (acl-1), Putative 1-acyl-sn-glycerol-3-phosphate acyltransferase acl-12 (acl-12)
  3. Phosphatidate cytidylyltransferase (cdgs-1), Phosphatidate cytidylyltransferase, mitochondrial (Y71F9B.2)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-CEL-1483148. Synthesis of PG.
R-CEL-1483226. Synthesis of PI.
UniPathwayiUPA00557; UER00614.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidate cytidylyltransferase (EC:2.7.7.41)
Alternative name(s):
CDP-DAG synthase
CDP-DG synthase
CDP-diacylglycerol synthase
CDP-diglyceride pyrophosphorylase
CDP-diglyceride synthase
Gene namesi
Name:cdgs-1
ORF Names:C33H5.18
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC33H5.18a; CE04161; WBGene00016384; cdgs-1.
C33H5.18b; CE26907; WBGene00016384; cdgs-1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei95 – 117HelicalSequence analysisAdd BLAST23
Transmembranei121 – 143HelicalSequence analysisAdd BLAST23
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21
Transmembranei214 – 234HelicalSequence analysisAdd BLAST21
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Transmembranei288 – 308HelicalSequence analysisAdd BLAST21
Transmembranei367 – 387HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Reduced levels of phosphatidylinositol. No effect on two-cell stage nuclear morphology. Significant rescue of the nuclear morphology defects resulting from inhibition of cnep-1 or partial inhibition of lpin-1 but does not rescue the nuclear envelope disassembly defects observed following depletion of npp-12.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000907191 – 465Phosphatidate cytidylyltransferaseAdd BLAST465

Proteomic databases

EPDiP53439.
PaxDbiP53439.
PeptideAtlasiP53439.
PRIDEiP53439.

PTM databases

iPTMnetiP53439.

Expressioni

Gene expression databases

BgeeiWBGene00016384.

Interactioni

Protein-protein interaction databases

IntActiP53439. 1 interactor.
MINTiMINT-3386513.
STRINGi6239.C33H5.18b.

Structurei

3D structure databases

ProteinModelPortaliP53439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CDS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1440. Eukaryota.
COG0575. LUCA.
GeneTreeiENSGT00390000016175.
HOGENOMiHOG000209582.
InParanoidiP53439.
KOiK00981.
OMAiQRVAHEP.
OrthoDBiEOG091G06CA.
PhylomeDBiP53439.

Family and domain databases

InterProiIPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view]
PIRSFiPIRSF018269. PC_trans_euk. 1 hit.
PROSITEiPS01315. CDS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform b (identifier: P53439-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDQPPAENA DVRQRRAPES PVTERLRAPA RDDARPTSDE SDMEGILQDE
60 70 80 90 100
DSDAGSKNKE EKLERLTQAI PQDKGSLGVF ADSMLEALPP RWRNWVVRGL
110 120 130 140 150
FSIIMISTFT FIVTRGATWL MFLVFLIQFK CFQEIISIGL AVYRLYDFPW
160 170 180 190 200
FRALSWYFLL TSNYFFFGES LIDYWGIVLK KDNFLHFLVA YHRLVSFALY
210 220 230 240 250
CIGFVSFVLS LRKGYYMRQF SLFAWTHLTL LLIVSQSFFI IQNIFQGLIW
260 270 280 290 300
FLAPVAMIIC CDIMSYMFGF FWGKTPLIKL SPKKTWEGFI GGAFSTVVFG
310 320 330 340 350
ILLSLALYNR PFFVCPVQHY QTDSSNCTIP LAFQLQDYPV PRPFSFVYKI
360 370 380 390 400
LRKEPIIQLC PFVFHSIALS LFASILGPFG GFFASGFKRA FKIKDFGDVI
410 420 430 440 450
PGHGGLMDRF DCQLLMGTFV MVYIHSFIRV PDASKLLKQI MTLEPQDQLN
460
IFNLLQSELS KTGLI
Note: No experimental confirmation available.
Length:465
Mass (Da):53,460
Last modified:September 3, 2014 - v2
Checksum:iD02E7C5F17E9D3DD
GO
Isoform a (identifier: P53439-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-62: SDAGSKNKEEK → R

Note: No experimental confirmation available.
Show »
Length:455
Mass (Da):52,442
Checksum:iECD8048CA0A28649
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05541752 – 62SDAGSKNKEEK → R in isoform a. CuratedAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080768 Genomic DNA. Translation: CCD66581.1.
FO080768 Genomic DNA. Translation: CCD66582.1.
PIRiT34155.
RefSeqiNP_501297.1. NM_068896.3. [P53439-2]
NP_501298.1. NM_068897.3. [P53439-1]
UniGeneiCel.17593.

Genome annotation databases

EnsemblMetazoaiC33H5.18b; C33H5.18b; WBGene00016384. [P53439-1]
GeneIDi3565046.
KEGGicel:CELE_C33H5.18.
UCSCiC33H5.18b. c. elegans. [P53439-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080768 Genomic DNA. Translation: CCD66581.1.
FO080768 Genomic DNA. Translation: CCD66582.1.
PIRiT34155.
RefSeqiNP_501297.1. NM_068896.3. [P53439-2]
NP_501298.1. NM_068897.3. [P53439-1]
UniGeneiCel.17593.

3D structure databases

ProteinModelPortaliP53439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP53439. 1 interactor.
MINTiMINT-3386513.
STRINGi6239.C33H5.18b.

PTM databases

iPTMnetiP53439.

Proteomic databases

EPDiP53439.
PaxDbiP53439.
PeptideAtlasiP53439.
PRIDEiP53439.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC33H5.18b; C33H5.18b; WBGene00016384. [P53439-1]
GeneIDi3565046.
KEGGicel:CELE_C33H5.18.
UCSCiC33H5.18b. c. elegans. [P53439-1]

Organism-specific databases

CTDi3565046.
WormBaseiC33H5.18a; CE04161; WBGene00016384; cdgs-1.
C33H5.18b; CE26907; WBGene00016384; cdgs-1.

Phylogenomic databases

eggNOGiKOG1440. Eukaryota.
COG0575. LUCA.
GeneTreeiENSGT00390000016175.
HOGENOMiHOG000209582.
InParanoidiP53439.
KOiK00981.
OMAiQRVAHEP.
OrthoDBiEOG091G06CA.
PhylomeDBiP53439.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00614.
ReactomeiR-CEL-1483148. Synthesis of PG.
R-CEL-1483226. Synthesis of PI.

Miscellaneous databases

PROiP53439.

Gene expression databases

BgeeiWBGene00016384.

Family and domain databases

InterProiIPR000374. PC_trans.
IPR016720. PC_Trfase_euk.
[Graphical view]
PIRSFiPIRSF018269. PC_trans_euk. 1 hit.
PROSITEiPS01315. CDS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDSA_CAEEL
AccessioniPrimary (citable) accession number: P53439
Secondary accession number(s): G8JY50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 3, 2014
Last modified: November 2, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.