P53430 (G3P_MONPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.12

Gene names

Name:

GPD1

Organism

Monascus purpureus (Monascus anka)

Taxonomic identifier

5098 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Elaphomycetaceae › Monascus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000145562
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	151 – 153	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	211 – 212	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	152	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	80	1	NAD; via carbonyl oxygen By similarity
	Binding site	182	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	234	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	316	1	NAD By similarity
	Site	179	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53430 [UniParc]. Last modified April 30, 2003. Version 3. Checksum: 2C1E4035EFF5A142	FASTA	337	36,402
	10 20 30 40 50 60 MVVPKVGING FGRIGRIVFR NAIEHEGVDI VAVNDPFIEV HYAAYMLKYD STHGRFNGTV 70 80 90 100 110 120 EFDGNTLIVN GKKIKFYAER DPAQIPWSET GAEYVVESTG VFTKQEKASL HLKGGAKKVI 130 140 150 160 170 180 ISAPSSDSPM FVMGVNNDQY TKDITVLSNA SCTTNCLAPL AKVINDKFGI VEGLMTTVHS 190 200 210 220 230 240 YTATQKVVDG PSNKDWRGGR TAAQNIIPSS TGAAKAVGKV IPSLNGKLTG MSMRVPTSNV 250 260 270 280 290 300 SVVDLTARLE KAATYDEIKQ AVKEASEGPL KGVLGYTEDD VVSSDLNGDP HSSIFDAKAG 310 320 330 IALNSNFVKL VSWYDNEWGY SRRVIDLIAY IAQVDAQ

References

[1]	"Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase (gpd1) from Monascus anka IFO4478." Kim J., Choi Y.D., Kim S.U. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 16360 / CBS 283.34 / JCM 6934 / NBRC 4478.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z68498 Genomic DNA. Translation: CAA92807.3.
3D structure databases
ProteinModelPortal	P53430.
SMR	P53430. Positions 5-332.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00109; UER00184.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10836. PTHR10836. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_MONPU

Accession

Primary (citable) accession number: P53430

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	April 30, 2003
Last modified:	March 6, 2013
This is version 82 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program