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Reviewed, UniProtKB/Swiss-Prot P53429 (G3P1_GIALA)

Last modified June 16, 2009. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH
    EC=1.2.1.12
Gene names
Name: GAP1
OrganismGiardia lamblia (Giardia intestinalis)
Taxonomic identifier5741 [NCBI]
Taxonomic lineageEukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia

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Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

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Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD or NADH binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Glyceraldehyde-3-phosphate dehydrogenase 1
PRO_0000145526

Regions

Nucleotide binding12 – 132NAD By similarity
Region151 – 1533Glyceraldehyde 3-phosphate binding By similarity
Region211 – 2122Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1521Nucleophile By similarity
Binding site341NAD By similarity
Binding site791NAD; via carbonyl oxygen By similarity
Binding site1821Glyceraldehyde 3-phosphate By similarity
Binding site2341Glyceraldehyde 3-phosphate By similarity
Binding site3161NAD By similarity
Site1791Activates thiol group during catalysis By similarity

Natural variations

Natural variant2141A → P in strain: ATCC 30957 / WB.
Natural variant2681N → K in strain: ATCC 30957 / WB.

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Sequences

Sequence LengthMass (Da)Tools
P53429-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 762F1AE90143B462

FASTA33736,450
        10         20         30         40         50         60 
MPIRLGINGF GRIGRMALRA SLNIDGVQVV AINDPFTDCE YMEYMLKYDT VHGRFDGTIA 

        70         80         90        100        110        120 
HSEDSITVNG NKISVFKSMK PEEIPWGKTQ VDIVLECTGR FTTKKDAELH ITGGCKRVII 

       130        140        150        160        170        180 
SAPSADAPMF VCGCNLETYD PSTMKVISNA SCTTNCLAPL AMVVNKKFGI KEGLMTTVHA 

       190        200        210        220        230        240 
VTATQLPVDG PSKKDWRGGR SCGANVIPSS TGAAKAVGKV LPALNGKLTG MAFRVPVPDV 

       250        260        270        280        290        300 
SVVDLTCTLE KDATYDEICA EIKRGSENEL KGIMTYTNED VVSSDFLSTT STCNFDSKAG 

       310        320        330 
IMLNSRFVKL VAWYDNEFGY ANKLVELAKY VGSKGCQ

« Hide

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References

[1]Rozario C., Smith M.W., Muller M.
Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 30957 / WB.
[2]Smith M.W., Doolittle R.F.
Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-312.
Strain: ATCC 30957 / WB.

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Cross-references

Sequence databases

M88062 Genomic DNA. Translation: AAB18421.1.

3D structure databases

HSSPHSSP built from PDB template 1CRW based on UniProtKB P56649.
ModBaseSearch...

Enzyme and pathway databases

BRENDA1.2.1.12. 322.

Family and domain databases

InterProIPR000173. GlycerAld_3-P_DH.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
PRINTSPR00078. G3PDHDRGNASE.
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameG3P1_GIALA
AccessionPrimary (citable) accession number: P53429
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents