Reviewed, UniProtKB/Swiss-Prot P53429 (G3P1_GIALA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH
    EC=1.2.1.12

Gene names

Name:

GAP1

Organism

Giardia lamblia (Giardia intestinalis)

Taxonomic identifier

5741 [NCBI]

Taxonomic lineage

Eukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Glyceraldehyde-3-phosphate dehydrogenase 1		PRO_0000145526
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	151 – 153	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	211 – 212	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	152	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	79	1	NAD; via carbonyl oxygen By similarity
	Binding site	182	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	234	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	316	1	NAD By similarity
	Site	179	1	Activates thiol group during catalysis By similarity
Natural variations
	Natural variant	214	1	A → P in strain: ATCC 30957 / WB.
	Natural variant	268	1	N → K in strain: ATCC 30957 / WB.

Sequences

	Sequence		Length	Mass (Da)
	P53429-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 762F1AE90143B462	FASTA	337	36,450
	10 20 30 40 50 60 MPIRLGINGF GRIGRMALRA SLNIDGVQVV AINDPFTDCE YMEYMLKYDT VHGRFDGTIA 70 80 90 100 110 120 HSEDSITVNG NKISVFKSMK PEEIPWGKTQ VDIVLECTGR FTTKKDAELH ITGGCKRVII 130 140 150 160 170 180 SAPSADAPMF VCGCNLETYD PSTMKVISNA SCTTNCLAPL AMVVNKKFGI KEGLMTTVHA 190 200 210 220 230 240 VTATQLPVDG PSKKDWRGGR SCGANVIPSS TGAAKAVGKV LPALNGKLTG MAFRVPVPDV 250 260 270 280 290 300 SVVDLTCTLE KDATYDEICA EIKRGSENEL KGIMTYTNED VVSSDFLSTT STCNFDSKAG 310 320 330 IMLNSRFVKL VAWYDNEFGY ANKLVELAKY VGSKGCQ

References

[1]	Rozario C., Smith M.W., Muller M. Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 30957 / WB.
[2]	Smith M.W., Doolittle R.F. Submitted (MAR-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 14-312. Strain: ATCC 30957 / WB.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M88062 Genomic DNA. Translation: AAB18421.1.
3D structure databases
HSSP	HSSP built from PDB template 1CRW based on UniProtKB P56649.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_GIALA

Accession

Primary (citable) accession number: P53429

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)