ID   CHS2_SECCE              Reviewed;         394 AA.
AC   P53415;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 83.
DE   RecName: Full=Chalcone synthase 2;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase 2;
GN   Name=CHS2;
OS   Secale cereale (Rye).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Secale.
OX   NCBI_TaxID=4550;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kustro;
RA   Haussuehl K.K., Rohde W., Weissenboeck G.;
RT   "Expression of chalcone synthase genes in coleoptiles and primary leaves of
RT   Secale cereale L. after induction by UV radiation: evidence for a UV-
RT   protective role of the coleoptile.";
RL   Bot. Acta 109:229-238(1996).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-coumaroyl-CoA + 2 H(+) + 3 malonyl-CoA = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57355, ChEBI:CHEBI:57384, ChEBI:CHEBI:77645; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; X92547; CAA63305.1; -; mRNA.
DR   AlphaFoldDB; P53415; -.
DR   SMR; P53415; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0102128; F:chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00831; CHS_like; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF95; CHAL_STI_SYNT_C DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..394
FT                   /note="Chalcone synthase 2"
FT                   /id="PRO_0000216049"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   394 AA;  43224 MW;  56A44FF0BD457B1F CRC64;
     MAATMTVEEV RKAQRAEGPA TVLAIGTATP ANCVYQADYP DYYFKITKSD HMADLKEKFK
     RMCDKSQIRK RYMHLTEEIL QDNPNMCAYM APSLDARQDI VVVEVPKLGK AAAQKAIKEW
     GQPRSKITHL VFCTTSGVDM PGADYQLTKM LGLRPSVKRL MMYQQGCFAG GTVLRLAKDL
     AENNRGARVL VVCSEITAVT FRGPHESHLD SLVGQALFGD GAAAVIIGAD PDESIERPLF
     QLVSASQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIERALEDAF KPLGIDDWNS
     VFWIAHPGGP AILDMVEAKV NLNKERMRAT RHVLSEYGNM SSACVLFIMD EMRKRSAEDG
     HTTTGEGMDW GVLFGFGPGL TVETVVLHSV PVTA
//