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P53397

- OGG1_YEAST

UniProt

P53397 - OGG1_YEAST

Protein

N-glycosylase/DNA lyase

Gene

OGG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei134 – 1341DNABy similarity
    Binding sitei139 – 1391DNABy similarity
    Binding sitei189 – 1891DNABy similarity
    Active sitei241 – 2411Schiff-base intermediate with DNA
    Binding sitei258 – 25818-oxoguanine; via carbonyl oxygenBy similarity
    Binding sitei260 – 26018-oxoguanineBy similarity
    Binding sitei262 – 2621DNABy similarity
    Binding sitei320 – 32018-oxoguanineBy similarity
    Binding sitei324 – 32418-oxoguanineBy similarity

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: SGD

    GO - Biological processi

    1. base-excision repair, AP site formation Source: SGD
    2. DNA catabolic process, endonucleolytic Source: GOC
    3. DNA repair Source: SGD
    4. error-free translesion synthesis Source: SGD
    5. nucleotide-excision repair Source: InterPro
    6. telomere maintenance via telomerase Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32655-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-glycosylase/DNA lyase
    Including the following 2 domains:
    8-oxoguanine DNA glycosylase (EC:3.2.2.-)
    DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
    Short name:
    AP lyase
    Gene namesi
    Name:OGG1
    Ordered Locus Names:YML060W
    ORF Names:YM9958.02
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML060w.
    SGDiS000004525. OGG1.

    Subcellular locationi

    GO - Cellular componenti

    1. CBF3 complex Source: SGD
    2. mitochondrion Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi241 – 2411K → Q: Abolishes both DNA glycosylase and AP lyase activity. 2 Publications
    Mutagenesisi241 – 2411K → R: Diminishes both DNA glycosylase and AP lyase activity. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 376376N-glycosylase/DNA lyasePRO_0000058595Add
    BLAST

    Proteomic databases

    MaxQBiP53397.
    PaxDbiP53397.

    Expressioni

    Gene expression databases

    GenevestigatoriP53397.

    Interactioni

    Protein-protein interaction databases

    BioGridi35106. 38 interactions.
    DIPiDIP-4538N.
    MINTiMINT-566071.
    STRINGi4932.YML060W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53397.
    SMRiP53397. Positions 12-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the type-1 OGG1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0122.
    GeneTreeiENSGT00640000091554.
    HOGENOMiHOG000180756.
    KOiK03660.
    OMAiSELCLAN.
    OrthoDBiEOG7TQV9T.

    Family and domain databases

    Gene3Di1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    InterProiIPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR023170. HTH_base_excis_C.
    IPR004577. Ogg.
    IPR012904. OGG_N.
    [Graphical view]
    PfamiPF00730. HhH-GPD. 1 hit.
    PF07934. OGG_N. 1 hit.
    [Graphical view]
    SMARTiSM00478. ENDO3c. 1 hit.
    [Graphical view]
    SUPFAMiSSF48150. SSF48150. 1 hit.
    TIGRFAMsiTIGR00588. ogg. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53397-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSYKFGKLAI NKSELCLANV LQAGQSFRWI WDEKLNQYST TMKIGQQEKY    50
    SVVILRQDEE NEILEFVAVG DCGNQDALKT HLMKYFRLDV SLKHLFDNVW 100
    IPSDKAFAKL SPQGIRILAQ EPWETLISFI CSSNNNISRI TRMCNSLCSN 150
    FGNLITTIDG VAYHSFPTSE ELTSRATEAK LRELGFGYRA KYIIETARKL 200
    VNDKAEANIT SDTTYLQSIC KDAQYEDVRE HLMSYNGVGP KVADCVCLMG 250
    LHMDGIVPVD VHVSRIAKRD YQISANKNHL KELRTKYNAL PISRKKINLE 300
    LDHIRLMLFK KWGSYAGWAQ GVLFSKEIGG TSGSTTTGTI KKRKWDMIKE 350
    TEAIVTKQMK LKVELSDLHI KEAKID 376
    Length:376
    Mass (Da):42,781
    Last modified:October 1, 1996 - v1
    Checksum:i4ADB72EB20E4BF20
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U44855 Genomic DNA. Translation: AAC49312.1.
    Z46729 Genomic DNA. Translation: CAA86715.1.
    BK006946 Genomic DNA. Translation: DAA09837.1.
    PIRiS49801.
    RefSeqiNP_013651.1. NM_001182419.1.

    Genome annotation databases

    EnsemblFungiiYML060W; YML060W; YML060W.
    GeneIDi854942.
    KEGGisce:YML060W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U44855 Genomic DNA. Translation: AAC49312.1 .
    Z46729 Genomic DNA. Translation: CAA86715.1 .
    BK006946 Genomic DNA. Translation: DAA09837.1 .
    PIRi S49801.
    RefSeqi NP_013651.1. NM_001182419.1.

    3D structure databases

    ProteinModelPortali P53397.
    SMRi P53397. Positions 12-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35106. 38 interactions.
    DIPi DIP-4538N.
    MINTi MINT-566071.
    STRINGi 4932.YML060W.

    Proteomic databases

    MaxQBi P53397.
    PaxDbi P53397.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML060W ; YML060W ; YML060W .
    GeneIDi 854942.
    KEGGi sce:YML060W.

    Organism-specific databases

    CYGDi YML060w.
    SGDi S000004525. OGG1.

    Phylogenomic databases

    eggNOGi COG0122.
    GeneTreei ENSGT00640000091554.
    HOGENOMi HOG000180756.
    KOi K03660.
    OMAi SELCLAN.
    OrthoDBi EOG7TQV9T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32655-MONOMER.

    Miscellaneous databases

    NextBioi 977995.
    PROi P53397.

    Gene expression databases

    Genevestigatori P53397.

    Family and domain databases

    Gene3Di 1.10.1670.10. 1 hit.
    1.10.340.30. 1 hit.
    InterProi IPR011257. DNA_glycosylase.
    IPR003265. HhH-GPD_domain.
    IPR023170. HTH_base_excis_C.
    IPR004577. Ogg.
    IPR012904. OGG_N.
    [Graphical view ]
    Pfami PF00730. HhH-GPD. 1 hit.
    PF07934. OGG_N. 1 hit.
    [Graphical view ]
    SMARTi SM00478. ENDO3c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48150. SSF48150. 1 hit.
    TIGRFAMsi TIGR00588. ogg. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine."
      van der Kemp P.A., Thomas D., Barbey R., de Oliveira R., Boiteux S.
      Proc. Natl. Acad. Sci. U.S.A. 93:5197-5202(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The Ogg1 protein of Saccharomyces cerevisiae: a 7,8-dihydro-8-oxoguanine DNA glycosylase/AP lyase whose lysine 241 is a critical residue for catalytic activity."
      Girard P.-M., Guibourt N., Boiteux S.
      Nucleic Acids Res. 25:3204-3211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF LYS-241.
    5. "Catalytic and DNA binding properties of the ogg1 protein of Saccharomyces cerevisiae: comparison between the wild type and the K241R and K241Q active-site mutant proteins."
      Guibourt N., Castaing B., Van Der Kemp P.A., Boiteux S.
      Biochemistry 39:1716-1724(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, MUTAGENESIS OF LYS-241.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOGG1_YEAST
    AccessioniPrimary (citable) accession number: P53397
    Secondary accession number(s): D6VZB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3690 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3