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P53397

- OGG1_YEAST

UniProt

P53397 - OGG1_YEAST

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Protein
N-glycosylase/DNA lyase
Gene
OGG1, YML060W, YM9958.02
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei134 – 1341DNA By similarity
Binding sitei139 – 1391DNA By similarity
Binding sitei189 – 1891DNA By similarity
Active sitei241 – 2411Schiff-base intermediate with DNA
Binding sitei258 – 25818-oxoguanine; via carbonyl oxygen By similarity
Binding sitei260 – 26018-oxoguanine By similarity
Binding sitei262 – 2621DNA By similarity
Binding sitei320 – 32018-oxoguanine By similarity
Binding sitei324 – 32418-oxoguanine By similarity

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: SGD

GO - Biological processi

  1. DNA catabolic process, endonucleolytic Source: GOC
  2. DNA repair Source: SGD
  3. base-excision repair, AP site formation Source: SGD
  4. error-free translesion synthesis Source: SGD
  5. nucleotide-excision repair Source: InterPro
  6. telomere maintenance via telomerase Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32655-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Including the following 2 domains:
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Name:OGG1
Ordered Locus Names:YML060W
ORF Names:YM9958.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYML060w.
SGDiS000004525. OGG1.

Subcellular locationi

GO - Cellular componenti

  1. CBF3 complex Source: SGD
  2. mitochondrion Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi241 – 2411K → Q: Abolishes both DNA glycosylase and AP lyase activity. 2 Publications
Mutagenesisi241 – 2411K → R: Diminishes both DNA glycosylase and AP lyase activity. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376N-glycosylase/DNA lyase
PRO_0000058595Add
BLAST

Proteomic databases

MaxQBiP53397.
PaxDbiP53397.

Expressioni

Gene expression databases

GenevestigatoriP53397.

Interactioni

Protein-protein interaction databases

BioGridi35106. 38 interactions.
DIPiDIP-4538N.
MINTiMINT-566071.
STRINGi4932.YML060W.

Structurei

3D structure databases

ProteinModelPortaliP53397.
SMRiP53397. Positions 12-328.

Family & Domainsi

Sequence similaritiesi

Belongs to the type-1 OGG1 family.

Phylogenomic databases

eggNOGiCOG0122.
GeneTreeiENSGT00640000091554.
HOGENOMiHOG000180756.
KOiK03660.
OMAiSELCLAN.
OrthoDBiEOG7TQV9T.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
IPR004577. Ogg.
IPR012904. OGG_N.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
PF07934. OGG_N. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00588. ogg. 1 hit.

Sequencei

Sequence statusi: Complete.

P53397-1 [UniParc]FASTAAdd to Basket

« Hide

MSYKFGKLAI NKSELCLANV LQAGQSFRWI WDEKLNQYST TMKIGQQEKY    50
SVVILRQDEE NEILEFVAVG DCGNQDALKT HLMKYFRLDV SLKHLFDNVW 100
IPSDKAFAKL SPQGIRILAQ EPWETLISFI CSSNNNISRI TRMCNSLCSN 150
FGNLITTIDG VAYHSFPTSE ELTSRATEAK LRELGFGYRA KYIIETARKL 200
VNDKAEANIT SDTTYLQSIC KDAQYEDVRE HLMSYNGVGP KVADCVCLMG 250
LHMDGIVPVD VHVSRIAKRD YQISANKNHL KELRTKYNAL PISRKKINLE 300
LDHIRLMLFK KWGSYAGWAQ GVLFSKEIGG TSGSTTTGTI KKRKWDMIKE 350
TEAIVTKQMK LKVELSDLHI KEAKID 376
Length:376
Mass (Da):42,781
Last modified:October 1, 1996 - v1
Checksum:i4ADB72EB20E4BF20
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U44855 Genomic DNA. Translation: AAC49312.1.
Z46729 Genomic DNA. Translation: CAA86715.1.
BK006946 Genomic DNA. Translation: DAA09837.1.
PIRiS49801.
RefSeqiNP_013651.1. NM_001182419.1.

Genome annotation databases

EnsemblFungiiYML060W; YML060W; YML060W.
GeneIDi854942.
KEGGisce:YML060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U44855 Genomic DNA. Translation: AAC49312.1 .
Z46729 Genomic DNA. Translation: CAA86715.1 .
BK006946 Genomic DNA. Translation: DAA09837.1 .
PIRi S49801.
RefSeqi NP_013651.1. NM_001182419.1.

3D structure databases

ProteinModelPortali P53397.
SMRi P53397. Positions 12-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35106. 38 interactions.
DIPi DIP-4538N.
MINTi MINT-566071.
STRINGi 4932.YML060W.

Proteomic databases

MaxQBi P53397.
PaxDbi P53397.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML060W ; YML060W ; YML060W .
GeneIDi 854942.
KEGGi sce:YML060W.

Organism-specific databases

CYGDi YML060w.
SGDi S000004525. OGG1.

Phylogenomic databases

eggNOGi COG0122.
GeneTreei ENSGT00640000091554.
HOGENOMi HOG000180756.
KOi K03660.
OMAi SELCLAN.
OrthoDBi EOG7TQV9T.

Enzyme and pathway databases

BioCyci YEAST:G3O-32655-MONOMER.

Miscellaneous databases

NextBioi 977995.
PROi P53397.

Gene expression databases

Genevestigatori P53397.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProi IPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
IPR004577. Ogg.
IPR012904. OGG_N.
[Graphical view ]
Pfami PF00730. HhH-GPD. 1 hit.
PF07934. OGG_N. 1 hit.
[Graphical view ]
SMARTi SM00478. ENDO3c. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
TIGRFAMsi TIGR00588. ogg. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine."
    van der Kemp P.A., Thomas D., Barbey R., de Oliveira R., Boiteux S.
    Proc. Natl. Acad. Sci. U.S.A. 93:5197-5202(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The Ogg1 protein of Saccharomyces cerevisiae: a 7,8-dihydro-8-oxoguanine DNA glycosylase/AP lyase whose lysine 241 is a critical residue for catalytic activity."
    Girard P.-M., Guibourt N., Boiteux S.
    Nucleic Acids Res. 25:3204-3211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF LYS-241.
  5. "Catalytic and DNA binding properties of the ogg1 protein of Saccharomyces cerevisiae: comparison between the wild type and the K241R and K241Q active-site mutant proteins."
    Guibourt N., Castaing B., Van Der Kemp P.A., Boiteux S.
    Biochemistry 39:1716-1724(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS OF LYS-241.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOGG1_YEAST
AccessioniPrimary (citable) accession number: P53397
Secondary accession number(s): D6VZB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3690 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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