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Protein

N-glycosylase/DNA lyase

Gene

OGG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei134DNABy similarity1
Binding sitei139DNABy similarity1
Binding sitei189DNABy similarity1
Active sitei241Schiff-base intermediate with DNA1
Binding sitei2588-oxoguanine; via carbonyl oxygenBy similarity1
Binding sitei2608-oxoguanineBy similarity1
Binding sitei262DNABy similarity1
Binding sitei3208-oxoguanineBy similarity1
Binding sitei3248-oxoguanineBy similarity1

GO - Molecular functioni

GO - Biological processi

  • base-excision repair, AP site formation Source: SGD
  • DNA repair Source: SGD
  • error-free translesion synthesis Source: SGD
  • nucleotide-excision repair Source: InterPro
  • telomere maintenance via telomerase Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32655-MONOMER.
ReactomeiR-SCE-110329. Cleavage of the damaged pyrimidine.
R-SCE-110331. Cleavage of the damaged purine.
R-SCE-110357. Displacement of DNA glycosylase by APEX1.

Names & Taxonomyi

Protein namesi
Recommended name:
N-glycosylase/DNA lyase
Including the following 2 domains:
8-oxoguanine DNA glycosylase (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase (EC:4.2.99.18)
Short name:
AP lyase
Gene namesi
Name:OGG1
Ordered Locus Names:YML060W
ORF Names:YM9958.02
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML060W.
SGDiS000004525. OGG1.

Subcellular locationi

GO - Cellular componenti

  • CBF3 complex Source: SGD
  • mitochondrion Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi241K → Q: Abolishes both DNA glycosylase and AP lyase activity. 2 Publications1
Mutagenesisi241K → R: Diminishes both DNA glycosylase and AP lyase activity. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000585951 – 376N-glycosylase/DNA lyaseAdd BLAST376

Proteomic databases

MaxQBiP53397.
PRIDEiP53397.
TopDownProteomicsiP53397.

Interactioni

Protein-protein interaction databases

BioGridi35106. 36 interactors.
DIPiDIP-4538N.
MINTiMINT-566071.

Structurei

3D structure databases

ProteinModelPortaliP53397.
SMRiP53397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the type-1 OGG1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00640000091554.
HOGENOMiHOG000180756.
InParanoidiP53397.
KOiK03660.
OMAiWRENDEQ.
OrthoDBiEOG092C29TV.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
IPR004577. Ogg1.
IPR012904. OGG_N.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
PF07934. OGG_N. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00588. ogg. 1 hit.

Sequencei

Sequence statusi: Complete.

P53397-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYKFGKLAI NKSELCLANV LQAGQSFRWI WDEKLNQYST TMKIGQQEKY
60 70 80 90 100
SVVILRQDEE NEILEFVAVG DCGNQDALKT HLMKYFRLDV SLKHLFDNVW
110 120 130 140 150
IPSDKAFAKL SPQGIRILAQ EPWETLISFI CSSNNNISRI TRMCNSLCSN
160 170 180 190 200
FGNLITTIDG VAYHSFPTSE ELTSRATEAK LRELGFGYRA KYIIETARKL
210 220 230 240 250
VNDKAEANIT SDTTYLQSIC KDAQYEDVRE HLMSYNGVGP KVADCVCLMG
260 270 280 290 300
LHMDGIVPVD VHVSRIAKRD YQISANKNHL KELRTKYNAL PISRKKINLE
310 320 330 340 350
LDHIRLMLFK KWGSYAGWAQ GVLFSKEIGG TSGSTTTGTI KKRKWDMIKE
360 370
TEAIVTKQMK LKVELSDLHI KEAKID
Length:376
Mass (Da):42,781
Last modified:October 1, 1996 - v1
Checksum:i4ADB72EB20E4BF20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44855 Genomic DNA. Translation: AAC49312.1.
Z46729 Genomic DNA. Translation: CAA86715.1.
BK006946 Genomic DNA. Translation: DAA09837.1.
PIRiS49801.
RefSeqiNP_013651.1. NM_001182419.1.

Genome annotation databases

EnsemblFungiiYML060W; YML060W; YML060W.
GeneIDi854942.
KEGGisce:YML060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44855 Genomic DNA. Translation: AAC49312.1.
Z46729 Genomic DNA. Translation: CAA86715.1.
BK006946 Genomic DNA. Translation: DAA09837.1.
PIRiS49801.
RefSeqiNP_013651.1. NM_001182419.1.

3D structure databases

ProteinModelPortaliP53397.
SMRiP53397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35106. 36 interactors.
DIPiDIP-4538N.
MINTiMINT-566071.

Proteomic databases

MaxQBiP53397.
PRIDEiP53397.
TopDownProteomicsiP53397.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML060W; YML060W; YML060W.
GeneIDi854942.
KEGGisce:YML060W.

Organism-specific databases

EuPathDBiFungiDB:YML060W.
SGDiS000004525. OGG1.

Phylogenomic databases

GeneTreeiENSGT00640000091554.
HOGENOMiHOG000180756.
InParanoidiP53397.
KOiK03660.
OMAiWRENDEQ.
OrthoDBiEOG092C29TV.

Enzyme and pathway databases

BioCyciYEAST:G3O-32655-MONOMER.
ReactomeiR-SCE-110329. Cleavage of the damaged pyrimidine.
R-SCE-110331. Cleavage of the damaged purine.
R-SCE-110357. Displacement of DNA glycosylase by APEX1.

Miscellaneous databases

PROiP53397.

Family and domain databases

CDDicd00056. ENDO3c. 1 hit.
Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
InterProiIPR011257. DNA_glycosylase.
IPR003265. HhH-GPD_domain.
IPR023170. HTH_base_excis_C.
IPR004577. Ogg1.
IPR012904. OGG_N.
[Graphical view]
PfamiPF00730. HhH-GPD. 1 hit.
PF07934. OGG_N. 1 hit.
[Graphical view]
SMARTiSM00478. ENDO3c. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00588. ogg. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiOGG1_YEAST
AccessioniPrimary (citable) accession number: P53397
Secondary accession number(s): D6VZB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3690 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.