Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P53390 (MEP3_YEAST)

Last modified November 3, 2009. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Ammonium transporter MEP3
Gene names
Name: MEP3
Synonyms: AMT3
Ordered Locus Names: YPR138C
ORF Names: P9659.14
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length489 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity. Ref.1 Ref.4

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Present with 3820 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the ammonium transporter (TC 2.A.49) family. [View classification]

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 489489Ammonium transporter MEP3
PRO_0000139756

Regions

Topological domain1 – 1717Extracellular Potential
Transmembrane18 – 3821 Potential
Topological domain39 – 4810Cytoplasmic Potential
Transmembrane49 – 6921 Potential
Topological domain70 – 10839Extracellular Potential
Transmembrane109 – 12921 Potential
Topological domain130 – 13910Cytoplasmic Potential
Transmembrane140 – 16021 Potential
Topological domain161 – 17313Extracellular Potential
Transmembrane174 – 19421 Potential
Topological domain195 – 20915Cytoplasmic Potential
Transmembrane210 – 23021 Potential
Topological domain231 – 2399Extracellular Potential
Transmembrane240 – 26021 Potential
Topological domain261 – 2677Cytoplasmic Potential
Transmembrane268 – 28821 Potential
Topological domain2891Extracellular Potential
Transmembrane290 – 31021 Potential
Topological domain311 – 33020Cytoplasmic Potential
Transmembrane331 – 35121 Potential
Topological domain352 – 37221Extracellular Potential
Transmembrane373 – 39321 Potential
Topological domain394 – 48996Cytoplasmic Potential

Experimental info

Sequence conflict71H → P in AAT92794. Ref.3

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P53390-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2309338F36D96258

FASTA48953,690
        10         20         30         40         50         60 
MARGDGHLWT ETYDSSTVAF MILGAALVFF MVPGLGFLYS GLARRKSALA LIWVVIMATL 

        70         80         90        100        110        120 
VGILQWYFWG YSLAFSKTAT NNKFIGNLDS FGFRNVYGKI SDDSTYPELI YAIFQMMFMC 

       130        140        150        160        170        180 
VALSIIAGAT AERGKLFPHM VFLFVFATLV YCPITYWIWA PGGWAYQWGV LDWAGGGNIE 

       190        200        210        220        230        240 
ILSAVAGFVY SYFLGRRKEN LLINFRPHNV SMVTLGTSIL WFGWLLFNAA SSLSPNMRSV 

       250        260        270        280        290        300 
YAFMNTCLSA TTGGMTWCLL DYRSEKKWST VGLCSGIICG LVAATPSSGC ITLYGSLIQG 

       310        320        330        340        350        360 
IIAGVVCNFA TKIKYYLKVD DSLDLLAEHG IAGVVGLIFN ALFAADWVIG MDGTTKHKGG 

       370        380        390        400        410        420 
WLTHNWKQMY IQIAYIGASA GYCAVVTAII CFVLGKIPGV HLRVTEEAEA LGLDEDQIGE 

       430        440        450        460        470        480 
FAYDYVEVRR DYYQWGVDTD ALHTTCNGAN SASETNPTED SQNSSLSSAT VSGQNEKSNN 


PKLHHAKEA

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"A family of ammonium transporters in Saccharomyces cerevisiae."
Marini A.-M., Soussi-Boudekou S., Vissers S., Andre B.
Mol. Cell. Biol. 17:4282-4293(1997) [PubMed: 9234685] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: Sigma 1278B.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed: 9169875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane."
Soupene E., Ramirez R.M., Kustu S.
Mol. Cell. Biol. 21:5733-5741(2001) [PubMed: 11486013] [Abstract]
Cited for: FUNCTION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

U40829 Genomic DNA. Translation: AAB68278.1.
AY692775 Genomic DNA. Translation: AAT92794.1.
PIRS69027.
RefSeqNP_015464.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:4909N.
IntActP53390. 27 interactions.
STRINGP53390.

Genome annotation databases

EnsemblYPR138C; YPR138C; YPR138C; Saccharomyces cerevisiae. [Genome view]
GeneID856260.
GenomeReviewsGene locus YPR138C in contig U00094_GR.
KEGGsce:YPR138C.
NMPDRfig|4932.3.peg.6603.

Organism-specific databases

CYGDYPR138c.
SGDS000006342. MEP3.

Phylogenomic databases

HOGENOMP53390.
OMANVSMVTL.

Gene expression databases

ArrayExpressP53390.
GenevestigatorP53390.
GermOnlineYPR138C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
[Graphical view]
PANTHERPTHR11730. Ammonium_transpt. 1 hit.
PfamPF00909. Ammonium_transp. 1 hit.
[Graphical view]
TIGRFAMsTIGR00836. amt. 1 hit.
PROSITEPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio981551.

Hide | Top

Entry information

Entry nameMEP3_YEAST
AccessionPrimary (citable) accession number: P53390
Secondary accession number(s): Q6B2F5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 3, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents