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Protein

Ammonium transporter MEP3

Gene

MEP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity.2 Publications

Miscellaneous

Present with 3820 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD

Keywordsi

Biological processAmmonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34273-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP3
Gene namesi
Name:MEP3
Synonyms:AMT3
Ordered Locus Names:YPR138C
ORF Names:P9659.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR138C
SGDiS000006342 MEP3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17ExtracellularSequence analysisAdd BLAST17
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Topological domaini39 – 48CytoplasmicSequence analysis10
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 108ExtracellularSequence analysisAdd BLAST39
Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
Topological domaini130 – 139CytoplasmicSequence analysis10
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 173ExtracellularSequence analysisAdd BLAST13
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 209CytoplasmicSequence analysisAdd BLAST15
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
Topological domaini231 – 239ExtracellularSequence analysis9
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 267CytoplasmicSequence analysis7
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289ExtracellularSequence analysis1
Transmembranei290 – 310HelicalSequence analysisAdd BLAST21
Topological domaini311 – 330CytoplasmicSequence analysisAdd BLAST20
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352 – 372ExtracellularSequence analysisAdd BLAST21
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21
Topological domaini394 – 489CytoplasmicSequence analysisAdd BLAST96

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397561 – 489Ammonium transporter MEP3Add BLAST489

Proteomic databases

PaxDbiP53390
PRIDEiP53390

Interactioni

Protein-protein interaction databases

BioGridi36307, 47 interactors
DIPiDIP-4909N
IntActiP53390, 22 interactors
MINTiP53390
STRINGi4932.YPR138C

Structurei

3D structure databases

ProteinModelPortaliP53390
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064546
HOGENOMiHOG000017736
InParanoidiP53390
KOiK03320
OMAiMFAAMTW
OrthoDBiEOG092C1Z22

Family and domain databases

Gene3Di1.10.3430.10, 1 hit
InterProiView protein in InterPro
IPR029020 Ammonium/urea_transptr
IPR001905 Ammonium_transpt
IPR018047 Ammonium_transpt_CS
IPR024041 NH4_transpt_AmtB-like_dom
PfamiView protein in Pfam
PF00909 Ammonium_transp, 1 hit
TIGRFAMsiTIGR00836 amt, 1 hit
PROSITEiView protein in PROSITE
PS01219 AMMONIUM_TRANSP, 1 hit

Sequencei

Sequence statusi: Complete.

P53390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGDGHLWT ETYDSSTVAF MILGAALVFF MVPGLGFLYS GLARRKSALA
60 70 80 90 100
LIWVVIMATL VGILQWYFWG YSLAFSKTAT NNKFIGNLDS FGFRNVYGKI
110 120 130 140 150
SDDSTYPELI YAIFQMMFMC VALSIIAGAT AERGKLFPHM VFLFVFATLV
160 170 180 190 200
YCPITYWIWA PGGWAYQWGV LDWAGGGNIE ILSAVAGFVY SYFLGRRKEN
210 220 230 240 250
LLINFRPHNV SMVTLGTSIL WFGWLLFNAA SSLSPNMRSV YAFMNTCLSA
260 270 280 290 300
TTGGMTWCLL DYRSEKKWST VGLCSGIICG LVAATPSSGC ITLYGSLIQG
310 320 330 340 350
IIAGVVCNFA TKIKYYLKVD DSLDLLAEHG IAGVVGLIFN ALFAADWVIG
360 370 380 390 400
MDGTTKHKGG WLTHNWKQMY IQIAYIGASA GYCAVVTAII CFVLGKIPGV
410 420 430 440 450
HLRVTEEAEA LGLDEDQIGE FAYDYVEVRR DYYQWGVDTD ALHTTCNGAN
460 470 480
SASETNPTED SQNSSLSSAT VSGQNEKSNN PKLHHAKEA
Length:489
Mass (Da):53,690
Last modified:October 1, 1996 - v1
Checksum:i2309338F36D96258
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7H → P in AAT92794 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA Translation: AAB68278.1
AY692775 Genomic DNA Translation: AAT92794.1
BK006949 Genomic DNA Translation: DAA11552.1
PIRiS69027
RefSeqiNP_015464.1, NM_001184235.1

Genome annotation databases

EnsemblFungiiYPR138C; YPR138C; YPR138C
GeneIDi856260
KEGGisce:YPR138C

Similar proteinsi

Entry informationi

Entry nameiMEP3_YEAST
AccessioniPrimary (citable) accession number: P53390
Secondary accession number(s): D6W4D6, Q6B2F5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

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