Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ammonium transporter MEP3

Gene

MEP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity.2 Publications

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD
  • organic cation transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP3
Gene namesi
Name:MEP3
Synonyms:AMT3
Ordered Locus Names:YPR138C
ORF Names:P9659.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR138C.
SGDiS000006342. MEP3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1717ExtracellularSequence analysisAdd
BLAST
Transmembranei18 – 3821HelicalSequence analysisAdd
BLAST
Topological domaini39 – 4810CytoplasmicSequence analysis
Transmembranei49 – 6921HelicalSequence analysisAdd
BLAST
Topological domaini70 – 10839ExtracellularSequence analysisAdd
BLAST
Transmembranei109 – 12921HelicalSequence analysisAdd
BLAST
Topological domaini130 – 13910CytoplasmicSequence analysis
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Topological domaini161 – 17313ExtracellularSequence analysisAdd
BLAST
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 20915CytoplasmicSequence analysisAdd
BLAST
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Topological domaini231 – 2399ExtracellularSequence analysis
Transmembranei240 – 26021HelicalSequence analysisAdd
BLAST
Topological domaini261 – 2677CytoplasmicSequence analysis
Transmembranei268 – 28821HelicalSequence analysisAdd
BLAST
Topological domaini289 – 2891ExtracellularSequence analysis
Transmembranei290 – 31021HelicalSequence analysisAdd
BLAST
Topological domaini311 – 33020CytoplasmicSequence analysisAdd
BLAST
Transmembranei331 – 35121HelicalSequence analysisAdd
BLAST
Topological domaini352 – 37221ExtracellularSequence analysisAdd
BLAST
Transmembranei373 – 39321HelicalSequence analysisAdd
BLAST
Topological domaini394 – 48996CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Ammonium transporter MEP3PRO_0000139756Add
BLAST

Interactioni

Protein-protein interaction databases

BioGridi36307. 29 interactions.
DIPiDIP-4909N.
IntActiP53390. 19 interactions.
MINTiMINT-554327.

Structurei

3D structure databases

ProteinModelPortaliP53390.
SMRiP53390. Positions 14-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP53390.
KOiK03320.
OMAiATKIKYY.
OrthoDBiEOG092C1Z22.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGDGHLWT ETYDSSTVAF MILGAALVFF MVPGLGFLYS GLARRKSALA
60 70 80 90 100
LIWVVIMATL VGILQWYFWG YSLAFSKTAT NNKFIGNLDS FGFRNVYGKI
110 120 130 140 150
SDDSTYPELI YAIFQMMFMC VALSIIAGAT AERGKLFPHM VFLFVFATLV
160 170 180 190 200
YCPITYWIWA PGGWAYQWGV LDWAGGGNIE ILSAVAGFVY SYFLGRRKEN
210 220 230 240 250
LLINFRPHNV SMVTLGTSIL WFGWLLFNAA SSLSPNMRSV YAFMNTCLSA
260 270 280 290 300
TTGGMTWCLL DYRSEKKWST VGLCSGIICG LVAATPSSGC ITLYGSLIQG
310 320 330 340 350
IIAGVVCNFA TKIKYYLKVD DSLDLLAEHG IAGVVGLIFN ALFAADWVIG
360 370 380 390 400
MDGTTKHKGG WLTHNWKQMY IQIAYIGASA GYCAVVTAII CFVLGKIPGV
410 420 430 440 450
HLRVTEEAEA LGLDEDQIGE FAYDYVEVRR DYYQWGVDTD ALHTTCNGAN
460 470 480
SASETNPTED SQNSSLSSAT VSGQNEKSNN PKLHHAKEA
Length:489
Mass (Da):53,690
Last modified:October 1, 1996 - v1
Checksum:i2309338F36D96258
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti7 – 71H → P in AAT92794 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68278.1.
AY692775 Genomic DNA. Translation: AAT92794.1.
BK006949 Genomic DNA. Translation: DAA11552.1.
PIRiS69027.
RefSeqiNP_015464.1. NM_001184235.1.

Genome annotation databases

EnsemblFungiiYPR138C; YPR138C; YPR138C.
GeneIDi856260.
KEGGisce:YPR138C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68278.1.
AY692775 Genomic DNA. Translation: AAT92794.1.
BK006949 Genomic DNA. Translation: DAA11552.1.
PIRiS69027.
RefSeqiNP_015464.1. NM_001184235.1.

3D structure databases

ProteinModelPortaliP53390.
SMRiP53390. Positions 14-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36307. 29 interactions.
DIPiDIP-4909N.
IntActiP53390. 19 interactions.
MINTiMINT-554327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR138C; YPR138C; YPR138C.
GeneIDi856260.
KEGGisce:YPR138C.

Organism-specific databases

EuPathDBiFungiDB:YPR138C.
SGDiS000006342. MEP3.

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP53390.
KOiK03320.
OMAiATKIKYY.
OrthoDBiEOG092C1Z22.

Enzyme and pathway databases

BioCyciYEAST:G3O-34273-MONOMER.

Miscellaneous databases

PROiP53390.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP3_YEAST
AccessioniPrimary (citable) accession number: P53390
Secondary accession number(s): D6W4D6, Q6B2F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.