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Protein

Ammonium transporter MEP3

Gene

MEP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transporter for ammonium (both charged and uncharged NH3 and NH4) to use as a nitrogen source. The affinity of MEP2 is about twenty times higher than that of MEP1. MEP3 has the lowest affinity.2 Publications

GO - Molecular functioni

  • ammonium transmembrane transporter activity Source: SGD

GO - Biological processi

  • ammonium transport Source: SGD
  • nitrogen utilization Source: SGD
  • organic cation transport Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Ammonia transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34273-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ammonium transporter MEP3
Gene namesi
Name:MEP3
Synonyms:AMT3
Ordered Locus Names:YPR138C
ORF Names:P9659.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR138C.
SGDiS000006342. MEP3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 17ExtracellularSequence analysisAdd BLAST17
Transmembranei18 – 38HelicalSequence analysisAdd BLAST21
Topological domaini39 – 48CytoplasmicSequence analysis10
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 108ExtracellularSequence analysisAdd BLAST39
Transmembranei109 – 129HelicalSequence analysisAdd BLAST21
Topological domaini130 – 139CytoplasmicSequence analysis10
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 173ExtracellularSequence analysisAdd BLAST13
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 209CytoplasmicSequence analysisAdd BLAST15
Transmembranei210 – 230HelicalSequence analysisAdd BLAST21
Topological domaini231 – 239ExtracellularSequence analysis9
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 267CytoplasmicSequence analysis7
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289ExtracellularSequence analysis1
Transmembranei290 – 310HelicalSequence analysisAdd BLAST21
Topological domaini311 – 330CytoplasmicSequence analysisAdd BLAST20
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352 – 372ExtracellularSequence analysisAdd BLAST21
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21
Topological domaini394 – 489CytoplasmicSequence analysisAdd BLAST96

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001397561 – 489Ammonium transporter MEP3Add BLAST489

Proteomic databases

PRIDEiP53390.

Interactioni

Protein-protein interaction databases

BioGridi36307. 29 interactors.
DIPiDIP-4909N.
IntActiP53390. 19 interactors.
MINTiMINT-554327.

Structurei

3D structure databases

ProteinModelPortaliP53390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP53390.
KOiK03320.
OMAiATKIKYY.
OrthoDBiEOG092C1Z22.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53390-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGDGHLWT ETYDSSTVAF MILGAALVFF MVPGLGFLYS GLARRKSALA
60 70 80 90 100
LIWVVIMATL VGILQWYFWG YSLAFSKTAT NNKFIGNLDS FGFRNVYGKI
110 120 130 140 150
SDDSTYPELI YAIFQMMFMC VALSIIAGAT AERGKLFPHM VFLFVFATLV
160 170 180 190 200
YCPITYWIWA PGGWAYQWGV LDWAGGGNIE ILSAVAGFVY SYFLGRRKEN
210 220 230 240 250
LLINFRPHNV SMVTLGTSIL WFGWLLFNAA SSLSPNMRSV YAFMNTCLSA
260 270 280 290 300
TTGGMTWCLL DYRSEKKWST VGLCSGIICG LVAATPSSGC ITLYGSLIQG
310 320 330 340 350
IIAGVVCNFA TKIKYYLKVD DSLDLLAEHG IAGVVGLIFN ALFAADWVIG
360 370 380 390 400
MDGTTKHKGG WLTHNWKQMY IQIAYIGASA GYCAVVTAII CFVLGKIPGV
410 420 430 440 450
HLRVTEEAEA LGLDEDQIGE FAYDYVEVRR DYYQWGVDTD ALHTTCNGAN
460 470 480
SASETNPTED SQNSSLSSAT VSGQNEKSNN PKLHHAKEA
Length:489
Mass (Da):53,690
Last modified:October 1, 1996 - v1
Checksum:i2309338F36D96258
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7H → P in AAT92794 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68278.1.
AY692775 Genomic DNA. Translation: AAT92794.1.
BK006949 Genomic DNA. Translation: DAA11552.1.
PIRiS69027.
RefSeqiNP_015464.1. NM_001184235.1.

Genome annotation databases

EnsemblFungiiYPR138C; YPR138C; YPR138C.
GeneIDi856260.
KEGGisce:YPR138C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40829 Genomic DNA. Translation: AAB68278.1.
AY692775 Genomic DNA. Translation: AAT92794.1.
BK006949 Genomic DNA. Translation: DAA11552.1.
PIRiS69027.
RefSeqiNP_015464.1. NM_001184235.1.

3D structure databases

ProteinModelPortaliP53390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36307. 29 interactors.
DIPiDIP-4909N.
IntActiP53390. 19 interactors.
MINTiMINT-554327.

Proteomic databases

PRIDEiP53390.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR138C; YPR138C; YPR138C.
GeneIDi856260.
KEGGisce:YPR138C.

Organism-specific databases

EuPathDBiFungiDB:YPR138C.
SGDiS000006342. MEP3.

Phylogenomic databases

GeneTreeiENSGT00530000064546.
HOGENOMiHOG000017736.
InParanoidiP53390.
KOiK03320.
OMAiATKIKYY.
OrthoDBiEOG092C1Z22.

Enzyme and pathway databases

BioCyciYEAST:G3O-34273-MONOMER.

Miscellaneous databases

PROiP53390.

Family and domain databases

Gene3Di1.10.3430.10. 1 hit.
InterProiIPR029020. Ammonium/urea_transptr.
IPR001905. Ammonium_transpt.
IPR018047. Ammonium_transpt_CS.
IPR024041. NH4_transpt_AmtB-like_dom.
[Graphical view]
PANTHERiPTHR11730. PTHR11730. 1 hit.
PfamiPF00909. Ammonium_transp. 1 hit.
[Graphical view]
SUPFAMiSSF111352. SSF111352. 1 hit.
TIGRFAMsiTIGR00836. amt. 1 hit.
PROSITEiPS01219. AMMONIUM_TRANSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMEP3_YEAST
AccessioniPrimary (citable) accession number: P53390
Secondary accession number(s): D6W4D6, Q6B2F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.