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Protein

Dicarboxylic amino acid permease

Gene

DIP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Can transport glutamate, aspartate, glutamine, asparagine, serine, alanine and glycine.1 Publication

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: SGD

GO - Biological processi

  • amino acid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-34148-MONOMER.

Protein family/group databases

TCDBi2.A.3.10.13. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Dicarboxylic amino acid permease
Gene namesi
Name:DIP5
Ordered Locus Names:YPL265W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL265W.
SGDiS000006186. DIP5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9090CytoplasmicSequence analysisAdd
BLAST
Transmembranei91 – 11121HelicalSequence analysisAdd
BLAST
Topological domaini112 – 1198ExtracellularSequence analysis
Transmembranei120 – 14223HelicalSequence analysisAdd
BLAST
Topological domaini143 – 15614CytoplasmicSequence analysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence analysisAdd
BLAST
Topological domaini178 – 20124ExtracellularSequence analysisAdd
BLAST
Transmembranei202 – 22221HelicalSequence analysisAdd
BLAST
Topological domaini223 – 2319CytoplasmicSequence analysis
Transmembranei232 – 25221HelicalSequence analysisAdd
BLAST
Topological domaini253 – 28533ExtracellularSequence analysisAdd
BLAST
Transmembranei286 – 30621HelicalSequence analysisAdd
BLAST
Topological domaini307 – 32216CytoplasmicSequence analysisAdd
BLAST
Transmembranei323 – 34321HelicalSequence analysisAdd
BLAST
Topological domaini344 – 37229ExtracellularSequence analysisAdd
BLAST
Transmembranei373 – 39321HelicalSequence analysisAdd
BLAST
Topological domaini394 – 42330CytoplasmicSequence analysisAdd
BLAST
Transmembranei424 – 44421HelicalSequence analysisAdd
BLAST
Topological domaini445 – 45410ExtracellularSequence analysis
Transmembranei455 – 47521HelicalSequence analysisAdd
BLAST
Topological domaini476 – 50126CytoplasmicSequence analysisAdd
BLAST
Transmembranei502 – 52221HelicalSequence analysisAdd
BLAST
Topological domaini523 – 5308ExtracellularSequence analysis
Transmembranei531 – 55121HelicalSequence analysisAdd
BLAST
Topological domaini552 – 60857CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Dicarboxylic amino acid permeasePRO_0000054150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei22 – 221PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53388.
PRIDEiP53388.

PTM databases

iPTMnetiP53388.

Interactioni

Protein-protein interaction databases

BioGridi35948. 59 interactions.
DIPiDIP-5540N.
IntActiP53388. 20 interactions.
MINTiMINT-499810.

Structurei

3D structure databases

ProteinModelPortaliP53388.
SMRiP53388. Positions 91-510.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi574 – 5774Poly-Glu

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP53388.
KOiK16261.
OMAiHLSIFLG.
OrthoDBiEOG74J9HZ.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53388-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMPLKKMFT STSPRNSSSL DSDHDAYYSK QNPDNFPVKE QEIYNIDLEE
60 70 80 90 100
NNVSSRSSTS TSPSARDDSF AVPDGKDENT RLRKDLKARH ISMIAIGGSL
110 120 130 140 150
GTGLLIGTGT ALLTGGPVAM LIAYAFVGLL VFYTMACLGE MASYIPLDGF
160 170 180 190 200
TSYASRYVDP ALGFAIGYTY LFKYFILPPN QLTAAALVIQ YWISRDRVNP
210 220 230 240 250
GVWITIFLVV IVAINVVGVK FFGEFEFWLS SFKVMVMLGL ILLLFIIMLG
260 270 280 290 300
GGPNHDRLGF RYWRDPGAFK EYSTAITGGK GKFVSFVAVF VYSLFSYTGI
310 320 330 340 350
ELTGIVCSEA ENPRKSVPKA IKLTVYRIIV FYLCTVFLLG MCVAYNDPRL
360 370 380 390 400
LSTKGKSMSA AASPFVVAIQ NSGIEVLPHI FNACVLVFVF SACNSDLYVS
410 420 430 440 450
SRNLYALAID GKAPKIFAKT SRWGVPYNAL ILSVLFCGLA YMNVSSGSAK
460 470 480 490 500
IFNYFVNVVS MFGILSWITI LIVYIYFDKA CRAQGIDKSK FAYVAPGQRY
510 520 530 540 550
GAYFALFFCI LIALIKNFTV FLGHKFDYKT FITGYIGLPV YIISWAGYKL
560 570 580 590 600
IYKTKVIKST DVDLYTFKEI YDREEEEGRM KDQEKEERLK SNGKNMEWFY

EKFLGNIF
Length:608
Mass (Da):68,097
Last modified:October 1, 1996 - v1
Checksum:i488A382656039D0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95802 Genomic DNA. Translation: CAA65074.1.
Z73621 Genomic DNA. Translation: CAA98000.1.
BK006949 Genomic DNA. Translation: DAA11171.1.
PIRiS65298.
RefSeqiNP_015058.1. NM_001184079.1.

Genome annotation databases

EnsemblFungiiYPL265W; YPL265W; YPL265W.
GeneIDi855863.
KEGGisce:YPL265W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95802 Genomic DNA. Translation: CAA65074.1.
Z73621 Genomic DNA. Translation: CAA98000.1.
BK006949 Genomic DNA. Translation: DAA11171.1.
PIRiS65298.
RefSeqiNP_015058.1. NM_001184079.1.

3D structure databases

ProteinModelPortaliP53388.
SMRiP53388. Positions 91-510.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35948. 59 interactions.
DIPiDIP-5540N.
IntActiP53388. 20 interactions.
MINTiMINT-499810.

Protein family/group databases

TCDBi2.A.3.10.13. the amino acid-polyamine-organocation (apc) family.

PTM databases

iPTMnetiP53388.

Proteomic databases

MaxQBiP53388.
PRIDEiP53388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL265W; YPL265W; YPL265W.
GeneIDi855863.
KEGGisce:YPL265W.

Organism-specific databases

EuPathDBiFungiDB:YPL265W.
SGDiS000006186. DIP5.

Phylogenomic databases

GeneTreeiENSGT00510000049744.
HOGENOMiHOG000261848.
InParanoidiP53388.
KOiK16261.
OMAiHLSIFLG.
OrthoDBiEOG74J9HZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-34148-MONOMER.

Miscellaneous databases

PROiP53388.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004762. Amino_acid_permease_fungi.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00913. 2A0310. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Vissers S., Grosjean S., Andre B.
    Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sigma 1278B.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Substrate specificity and gene expression of the amino-acid permeases in Saccharomyces cerevisiae."
    Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.
    Curr. Genet. 36:317-328(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDIP5_YEAST
AccessioniPrimary (citable) accession number: P53388
Secondary accession number(s): D6W3A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 432 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.