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P53384

- NUBP1_HUMAN

UniProt

P53384 - NUBP1_HUMAN

Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

NUBP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (01 Sep 2009)
      Previous versions | rss
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    Functioni

    Implicated in the regulation of centrosome duplication By similarity. Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins.By similarity1 Publication

    Cofactori

    Binds 4 4Fe-4S clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi8 – 81Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi22 – 221Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi25 – 251Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi31 – 311Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi235 – 2351Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation
    Metal bindingi238 – 2381Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 698ATPUniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. iron-sulfur cluster binding Source: UniProtKB
    4. metal ion binding Source: UniProtKB-KW
    5. nucleoside-triphosphatase activity Source: InterPro
    6. nucleotide binding Source: ProtInc
    7. protein binding Source: UniProtKB

    GO - Biological processi

    1. cell growth Source: UniProtKB
    2. cellular iron ion homeostasis Source: UniProtKB
    3. centrosome localization Source: Ensembl
    4. iron-sulfur cluster assembly Source: UniProtKB
    5. negative regulation of centrosome duplication Source: Ensembl
    6. protein localization to cell cortex Source: Ensembl
    7. small molecule metabolic process Source: Reactome

    Keywords - Ligandi

    4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_160176. Cytosolic iron-sulfur cluster assembly.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
    Alternative name(s):
    Nucleotide-binding protein 1UniRule annotation
    Short name:
    NBP 1UniRule annotation
    Gene namesi
    Name:NUBP1UniRule annotation
    Synonyms:NBP, NBP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 16

    Organism-specific databases

    HGNCiHGNC:8041. NUBP1.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: HPA
    2. cytosol Source: UniProtKB
    3. extracellular vesicular exosome Source: UniProt
    4. plasma membrane Source: HPA

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31823.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320Cytosolic Fe-S cluster assembly factor NUBP1PRO_0000184943Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine3 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP53384.
    PaxDbiP53384.
    PRIDEiP53384.

    PTM databases

    PhosphoSiteiP53384.

    Expressioni

    Gene expression databases

    ArrayExpressiP53384.
    BgeeiP53384.
    CleanExiHS_NUBP1.
    GenevestigatoriP53384.

    Organism-specific databases

    HPAiHPA041656.
    HPA041799.

    Interactioni

    Subunit structurei

    Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1 By similarity. Interacts with NUBP2.1 PublicationUniRule annotation

    Protein-protein interaction databases

    BioGridi110762. 19 interactions.
    IntActiP53384. 1 interaction.
    MINTiMINT-3019860.
    STRINGi9606.ENSP00000283027.

    Structurei

    3D structure databases

    ProteinModelPortaliP53384.
    SMRiP53384. Positions 44-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0489.
    HOGENOMiHOG000079916.
    HOVERGENiHBG051027.
    InParanoidiP53384.
    OMAiMCREMGV.
    PhylomeDBiP53384.
    TreeFamiTF300755.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_03038. NUBP1.
    InterProiIPR003593. AAA+_ATPase.
    IPR025723. Anion-transp_ATPase-like_dom.
    IPR019591. Mrp/NBP35_ATP-bd.
    IPR000808. Mrp_CS.
    IPR028601. NUBP1/Nbp35.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR23264. PTHR23264. 1 hit.
    PfamiPF02374. ArsA_ATPase. 1 hit.
    PF10609. ParA. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS01215. MRP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P53384-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEEVPHDCPG ADSAQAGRGA SCQGCPNQRL CASGAGATPD TAIEEIKEKM    50
    KTVKHKILVL SGKGGVGKST FSAHLAHGLA EDENTQIALL DIDICGPSIP 100
    KIMGLEGEQV HQSGSGWSPV YVEDNLGVMS VGFLLSSPDD AVIWRGPKKN 150
    GMIKQFLRDV DWGEVDYLIV DTPPGTSDEH LSVVRYLATA HIDGAVIITT 200
    PQEVSLQDVR KEINFCRKVK LPIIGVVENM SGFICPKCKK ESQIFPPTTG 250
    GAELMCQDLE VPLLGRVPLD PLIGKNCDKG QSFFIDAPDS PATLAYRSII 300
    QRIQEFCNLH QSKEENLISS 320
    Length:320
    Mass (Da):34,534
    Last modified:September 1, 2009 - v2
    Checksum:i346E1C694224E437
    GO
    Isoform 2 (identifier: P53384-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         110-120: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:309
    Mass (Da):33,412
    Checksum:i1A2201FF81478FFE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti204 – 2041V → L in AAA61932. (PubMed:7926816)Curated
    Sequence conflicti232 – 2321G → P in AAA61932. (PubMed:7926816)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti39 – 391P → A.2 Publications
    Corresponds to variant rs2233531 [ dbSNP | Ensembl ].
    VAR_020359

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei110 – 12011Missing in isoform 2. 1 PublicationVSP_029043Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01833 mRNA. Translation: AAA61932.1.
    AK223204 mRNA. Translation: BAD96924.1.
    BC100290 mRNA. Translation: AAI00291.1.
    BC109322 mRNA. Translation: AAI09323.1.
    BC109323 mRNA. Translation: AAI09324.1.
    CCDSiCCDS10543.1. [P53384-1]
    CCDS61839.1. [P53384-2]
    PIRiJC4010.
    RefSeqiNP_001265435.1. NM_001278506.1. [P53384-2]
    NP_002475.2. NM_002484.3. [P53384-1]
    UniGeneiHs.81469.

    Genome annotation databases

    EnsembliENST00000283027; ENSP00000283027; ENSG00000103274. [P53384-1]
    ENST00000433392; ENSP00000409654; ENSG00000103274. [P53384-2]
    GeneIDi4682.
    KEGGihsa:4682.
    UCSCiuc002daa.1. human. [P53384-1]
    uc002dab.1. human. [P53384-2]

    Polymorphism databases

    DMDMi257050984.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U01833 mRNA. Translation: AAA61932.1 .
    AK223204 mRNA. Translation: BAD96924.1 .
    BC100290 mRNA. Translation: AAI00291.1 .
    BC109322 mRNA. Translation: AAI09323.1 .
    BC109323 mRNA. Translation: AAI09324.1 .
    CCDSi CCDS10543.1. [P53384-1 ]
    CCDS61839.1. [P53384-2 ]
    PIRi JC4010.
    RefSeqi NP_001265435.1. NM_001278506.1. [P53384-2 ]
    NP_002475.2. NM_002484.3. [P53384-1 ]
    UniGenei Hs.81469.

    3D structure databases

    ProteinModelPortali P53384.
    SMRi P53384. Positions 44-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110762. 19 interactions.
    IntActi P53384. 1 interaction.
    MINTi MINT-3019860.
    STRINGi 9606.ENSP00000283027.

    PTM databases

    PhosphoSitei P53384.

    Polymorphism databases

    DMDMi 257050984.

    Proteomic databases

    MaxQBi P53384.
    PaxDbi P53384.
    PRIDEi P53384.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000283027 ; ENSP00000283027 ; ENSG00000103274 . [P53384-1 ]
    ENST00000433392 ; ENSP00000409654 ; ENSG00000103274 . [P53384-2 ]
    GeneIDi 4682.
    KEGGi hsa:4682.
    UCSCi uc002daa.1. human. [P53384-1 ]
    uc002dab.1. human. [P53384-2 ]

    Organism-specific databases

    CTDi 4682.
    GeneCardsi GC16P010837.
    H-InvDB HIX0038641.
    HGNCi HGNC:8041. NUBP1.
    HPAi HPA041656.
    HPA041799.
    MIMi 600280. gene.
    neXtProti NX_P53384.
    PharmGKBi PA31823.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0489.
    HOGENOMi HOG000079916.
    HOVERGENi HBG051027.
    InParanoidi P53384.
    OMAi MCREMGV.
    PhylomeDBi P53384.
    TreeFami TF300755.

    Enzyme and pathway databases

    Reactomei REACT_160176. Cytosolic iron-sulfur cluster assembly.

    Miscellaneous databases

    GenomeRNAii 4682.
    NextBioi 18050.
    PROi P53384.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53384.
    Bgeei P53384.
    CleanExi HS_NUBP1.
    Genevestigatori P53384.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_03038. NUBP1.
    InterProi IPR003593. AAA+_ATPase.
    IPR025723. Anion-transp_ATPase-like_dom.
    IPR019591. Mrp/NBP35_ATP-bd.
    IPR000808. Mrp_CS.
    IPR028601. NUBP1/Nbp35.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR23264. PTHR23264. 1 hit.
    Pfami PF02374. ArsA_ATPase. 1 hit.
    PF10609. ParA. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS01215. MRP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD."
      Shahrestanifar M., Saha D.P., Scala L.A., Basu A., Howells R.D.
      Gene 147:281-285(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-39.
      Tissue: Spleen.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-39.
      Tissue: Eye.
    4. "Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis."
      Stehling O., Netz D.J.A., Niggemeyer B., Roesser R., Eisenstein R.S., Puccio H., Pierik A.J., Lill R.
      Mol. Cell. Biol. 28:5517-5528(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NUBP2, SUBCELLULAR LOCATION, EPR SPECTROSCOPY OF IRON-SULFUR CLUSTERS.
    5. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNUBP1_HUMAN
    AccessioniPrimary (citable) accession number: P53384
    Secondary accession number(s): Q32M30, Q498A9, Q53FS7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 1, 2009
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 16
      Human chromosome 16: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3