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Protein

Cytosolic Fe-S cluster assembly factor NUBP1

Gene

NUBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery (PubMed:18573874). Required for maturation of extramitochondrial Fe-S proteins (PubMed:18573874). The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins (PubMed:18573874). Implicated in the regulation of centrosome duplication (By similarity). Negatively regulates cilium formation and structure (By similarity).By similarity1 Publication

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi22Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi25Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi31Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi235Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi238Iron-sulfur 2 (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 69ATPUniRule annotation8

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • ATP binding Source: UniProtKB-KW
  • iron-sulfur cluster binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • nucleotide binding Source: ProtInc

GO - Biological processi

  • cell growth Source: UniProtKB
  • cell projection organization Source: UniProtKB-KW
  • cellular iron ion homeostasis Source: UniProtKB
  • centrosome localization Source: Ensembl
  • iron-sulfur cluster assembly Source: UniProtKB
  • negative regulation of centrosome duplication Source: Ensembl
  • protein localization to cell cortex Source: Ensembl

Keywordsi

Biological processCilium biogenesis/degradation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-2564830. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1UniRule annotation
Alternative name(s):
Nucleotide-binding protein 1UniRule annotation
Short name:
NBP 1UniRule annotation
Gene namesi
Name:NUBP1UniRule annotation
Synonyms:NBP, NBP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:8041. NUBP1.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication
  • Nucleus By similarity
  • Cell projection By similarity
  • Cytoplasmcytoskeletoncilium axoneme By similarity
  • Cytoplasmcytoskeletoncilium basal body By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing center By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome

  • Note: Enriched in centrioles of microtubule asters during prophase, prometaphase and telophase stages of mitosis. Localized at centrioles and in the nucleus at interphase. Colocalizes with nubp-2 at prometaphase. Specifically localizes to the axenome of motile cilia as opposed to primary non-motile cilia. Localization is independent of NUBP2 and KIFC1.By similarity

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • cilium Source: UniProtKB-KW
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • nucleus Source: UniProtKB-SubCell
  • plasma membrane Source: HPA

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi4682.
OpenTargetsiENSG00000103274.
PharmGKBiPA31823.

Polymorphism and mutation databases

DMDMi257050984.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001849431 – 320Cytosolic Fe-S cluster assembly factor NUBP1Add BLAST320

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei319PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP53384.
MaxQBiP53384.
PaxDbiP53384.
PeptideAtlasiP53384.
PRIDEiP53384.

PTM databases

iPTMnetiP53384.
PhosphoSitePlusiP53384.

Expressioni

Gene expression databases

BgeeiENSG00000103274.
CleanExiHS_NUBP1.
ExpressionAtlasiP53384. baseline and differential.
GenevisibleiP53384. HS.

Organism-specific databases

HPAiHPA041656.
HPA041799.

Interactioni

Subunit structurei

Heterotetramer of 2 NUBP1 and 2 NUBP2 chains (By similarity). Interacts with KIFC1 (By similarity). Interacts with NUBP2 (PubMed:18573874). Interacts with the BBS/CCT complex subunit CCT1 (By similarity).UniRule annotationBy similarity1 Publication

Protein-protein interaction databases

BioGridi110762. 19 interactors.
IntActiP53384. 1 interactor.
MINTiMINT-3019860.
STRINGi9606.ENSP00000283027.

Structurei

3D structure databases

ProteinModelPortaliP53384.
SMRiP53384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00390000009735.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiP53384.
OMAiQRIREFC.
OrthoDBiEOG091G0DY5.
PhylomeDBiP53384.
TreeFamiTF300755.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
IPR033756. YlxH/NBP35.
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiView protein in Pfam
PF10609. ParA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS01215. MRP. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53384-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEEVPHDCPG ADSAQAGRGA SCQGCPNQRL CASGAGATPD TAIEEIKEKM
60 70 80 90 100
KTVKHKILVL SGKGGVGKST FSAHLAHGLA EDENTQIALL DIDICGPSIP
110 120 130 140 150
KIMGLEGEQV HQSGSGWSPV YVEDNLGVMS VGFLLSSPDD AVIWRGPKKN
160 170 180 190 200
GMIKQFLRDV DWGEVDYLIV DTPPGTSDEH LSVVRYLATA HIDGAVIITT
210 220 230 240 250
PQEVSLQDVR KEINFCRKVK LPIIGVVENM SGFICPKCKK ESQIFPPTTG
260 270 280 290 300
GAELMCQDLE VPLLGRVPLD PLIGKNCDKG QSFFIDAPDS PATLAYRSII
310 320
QRIQEFCNLH QSKEENLISS
Length:320
Mass (Da):34,534
Last modified:September 1, 2009 - v2
Checksum:i346E1C694224E437
GO
Isoform 2 (identifier: P53384-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-120: Missing.

Note: No experimental confirmation available.
Show »
Length:309
Mass (Da):33,412
Checksum:i1A2201FF81478FFE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti204V → L in AAA61932 (PubMed:7926816).Curated1
Sequence conflicti232G → P in AAA61932 (PubMed:7926816).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02035939P → A2 PublicationsCorresponds to variant dbSNP:rs2233531Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_029043110 – 120Missing in isoform 2. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01833 mRNA. Translation: AAA61932.1.
AK223204 mRNA. Translation: BAD96924.1.
BC100290 mRNA. Translation: AAI00291.1.
BC109322 mRNA. Translation: AAI09323.1.
BC109323 mRNA. Translation: AAI09324.1.
CCDSiCCDS10543.1. [P53384-1]
CCDS61839.1. [P53384-2]
PIRiJC4010.
RefSeqiNP_001265435.1. NM_001278506.1. [P53384-2]
NP_002475.2. NM_002484.3. [P53384-1]
UniGeneiHs.81469.

Genome annotation databases

EnsembliENST00000283027; ENSP00000283027; ENSG00000103274. [P53384-1]
ENST00000433392; ENSP00000409654; ENSG00000103274. [P53384-2]
GeneIDi4682.
KEGGihsa:4682.
UCSCiuc002daa.3. human. [P53384-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01833 mRNA. Translation: AAA61932.1.
AK223204 mRNA. Translation: BAD96924.1.
BC100290 mRNA. Translation: AAI00291.1.
BC109322 mRNA. Translation: AAI09323.1.
BC109323 mRNA. Translation: AAI09324.1.
CCDSiCCDS10543.1. [P53384-1]
CCDS61839.1. [P53384-2]
PIRiJC4010.
RefSeqiNP_001265435.1. NM_001278506.1. [P53384-2]
NP_002475.2. NM_002484.3. [P53384-1]
UniGeneiHs.81469.

3D structure databases

ProteinModelPortaliP53384.
SMRiP53384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110762. 19 interactors.
IntActiP53384. 1 interactor.
MINTiMINT-3019860.
STRINGi9606.ENSP00000283027.

PTM databases

iPTMnetiP53384.
PhosphoSitePlusiP53384.

Polymorphism and mutation databases

DMDMi257050984.

Proteomic databases

EPDiP53384.
MaxQBiP53384.
PaxDbiP53384.
PeptideAtlasiP53384.
PRIDEiP53384.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000283027; ENSP00000283027; ENSG00000103274. [P53384-1]
ENST00000433392; ENSP00000409654; ENSG00000103274. [P53384-2]
GeneIDi4682.
KEGGihsa:4682.
UCSCiuc002daa.3. human. [P53384-1]

Organism-specific databases

CTDi4682.
DisGeNETi4682.
GeneCardsiNUBP1.
H-InvDBHIX0038641.
HGNCiHGNC:8041. NUBP1.
HPAiHPA041656.
HPA041799.
MIMi600280. gene.
neXtProtiNX_P53384.
OpenTargetsiENSG00000103274.
PharmGKBiPA31823.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410KCSJ. Eukaryota.
COG0489. LUCA.
GeneTreeiENSGT00390000009735.
HOGENOMiHOG000079916.
HOVERGENiHBG051027.
InParanoidiP53384.
OMAiQRIREFC.
OrthoDBiEOG091G0DY5.
PhylomeDBiP53384.
TreeFamiTF300755.

Enzyme and pathway databases

ReactomeiR-HSA-2564830. Cytosolic iron-sulfur cluster assembly.

Miscellaneous databases

GenomeRNAii4682.
PROiP53384.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103274.
CleanExiHS_NUBP1.
ExpressionAtlasiP53384. baseline and differential.
GenevisibleiP53384. HS.

Family and domain databases

CDDicd02037. MRP-like. 1 hit.
Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_02040. Mrp_NBP35. 1 hit.
MF_03038. NUBP1. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR019591. Mrp/NBP35_ATP-bd.
IPR000808. Mrp_CS.
IPR028601. NUBP1/Nbp35.
IPR027417. P-loop_NTPase.
IPR033756. YlxH/NBP35.
PANTHERiPTHR23264. PTHR23264. 1 hit.
PfamiView protein in Pfam
PF10609. ParA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS01215. MRP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUBP1_HUMAN
AccessioniPrimary (citable) accession number: P53384
Secondary accession number(s): Q32M30, Q498A9, Q53FS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 1, 2009
Last modified: March 15, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.