P53384 (NUBP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic Fe-S cluster assembly factor NUBP1

Alternative name(s):
Nucleotide-binding protein 1
Short name=NBP 1

Gene names

Name:	NUBP1
Synonyms:	NBP, NBP1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the regulation of centrosome duplication By similarity. Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Ref.4
Cofactor	Binds 4 4Fe-4S clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer By similarity.
Subunit structure	Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1 By similarity. Interacts with NUBP2. Ref.4
Subcellular location	Cytoplasm Ref.4.
Sequence similarities	Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP1/NBP35 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell growth Inferred from mutant phenotype Ref.4. Source: UniProtKB cellular iron ion homeostasis Inferred from mutant phenotype Ref.4. Source: UniProtKB iron-sulfur cluster assembly Inferred from mutant phenotype Ref.4. Source: UniProtKB
Cellular_component	cytosol Inferred from direct assay Ref.4. Source: UniProtKB plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: UniProtKB-KW iron-sulfur cluster binding Inferred from direct assay Ref.4. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleotide binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P53384-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P53384-2)

The sequence of this isoform differs from the canonical sequence as follows:
     110-120: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Cytosolic Fe-S cluster assembly factor NUBP1 HAMAP-Rule MF_03038		PRO_0000184943
Regions
	Nucleotide binding	62 – 69	8	ATP Potential
Sites
	Metal binding	8	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	22	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	25	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	31	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	235	1	Iron-sulfur 2 (4Fe-4S); shared with dimeric partner By similarity
	Metal binding	238	1	Iron-sulfur 2 (4Fe-4S); shared with dimeric partner By similarity
Natural variations
	Alternative sequence	110 – 120	11	Missing in isoform 2.		VSP_029043
	Natural variant	39	1	P → A. Ref.2 Ref.3 Corresponds to variant rs2233531 [ dbSNP | Ensembl ].		VAR_020359
Experimental info
	Sequence conflict	204	1	V → L in AAA61932. Ref.1
	Sequence conflict	232	1	G → P in AAA61932. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 346E1C694224E437	FASTA	320	34,534
	10 20 30 40 50 60 MEEVPHDCPG ADSAQAGRGA SCQGCPNQRL CASGAGATPD TAIEEIKEKM KTVKHKILVL 70 80 90 100 110 120 SGKGGVGKST FSAHLAHGLA EDENTQIALL DIDICGPSIP KIMGLEGEQV HQSGSGWSPV 130 140 150 160 170 180 YVEDNLGVMS VGFLLSSPDD AVIWRGPKKN GMIKQFLRDV DWGEVDYLIV DTPPGTSDEH 190 200 210 220 230 240 LSVVRYLATA HIDGAVIITT PQEVSLQDVR KEINFCRKVK LPIIGVVENM SGFICPKCKK 250 260 270 280 290 300 ESQIFPPTTG GAELMCQDLE VPLLGRVPLD PLIGKNCDKG QSFFIDAPDS PATLAYRSII 310 320 QRIQEFCNLH QSKEENLISS
	Isoform 2 [UniParc]. Checksum: 1A2201FF81478FFE	FASTA	309	33,412
	10 20 30 40 50 60 MEEVPHDCPG ADSAQAGRGA SCQGCPNQRL CASGAGATPD TAIEEIKEKM KTVKHKILVL 70 80 90 100 110 120 SGKGGVGKST FSAHLAHGLA EDENTQIALL DIDICGPSIP KIMGLEGEQY VEDNLGVMSV 130 140 150 160 170 180 GFLLSSPDDA VIWRGPKKNG MIKQFLRDVD WGEVDYLIVD TPPGTSDEHL SVVRYLATAH 190 200 210 220 230 240 IDGAVIITTP QEVSLQDVRK EINFCRKVKL PIIGVVENMS GFICPKCKKE SQIFPPTTGG 250 260 270 280 290 300 AELMCQDLEV PLLGRVPLDP LIGKNCDKGQ SFFIDAPDSP ATLAYRSIIQ RIQEFCNLHQ SKEENLISS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a human cDNA encoding a putative nucleotide-binding protein related to Escherichia coli MinD." Shahrestanifar M., Saha D.P., Scala L.A., Basu A., Howells R.D. Gene 147:281-285(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ALA-39. Tissue: Spleen.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT ALA-39. Tissue: Eye.
[4]	"Human Nbp35 is essential for both cytosolic iron-sulfur protein assembly and iron homeostasis." Stehling O., Netz D.J.A., Niggemeyer B., Roesser R., Eisenstein R.S., Puccio H., Pierik A.J., Lill R. Mol. Cell. Biol. 28:5517-5528(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NUBP2, SUBCELLULAR LOCATION, EPR SPECTROSCOPY OF IRON-SULFUR CLUSTERS.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01833 mRNA. Translation: AAA61932.1. AK223204 mRNA. Translation: BAD96924.1. BC100290 mRNA. Translation: AAI00291.1. BC109322 mRNA. Translation: AAI09323.1. BC109323 mRNA. Translation: AAI09324.1.
IPI	IPI00021277. IPI00869040.
PIR	JC4010.
RefSeq	NP_002475.2. NM_002484.2.
UniGene	Hs.81469.
3D structure databases
ProteinModelPortal	P53384.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000283027.
PTM databases
PhosphoSite	P53384.
Polymorphism databases
DMDM	257050984.
Proteomic databases
PaxDb	P53384.
PRIDE	P53384.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000283027; ENSP00000283027; ENSG00000103274. ENST00000433392; ENSP00000409654; ENSG00000103274.
GeneID	4682.
KEGG	hsa:4682.
UCSC	uc002daa.1. human. uc002dab.1. human.
Organism-specific databases
CTD	4682.
GeneCards	GC16P010837.
H-InvDB	HIX0038641.
HGNC	HGNC:8041. NUBP1.
HPA	HPA041656. HPA041799.
MIM	600280. gene.
neXtProt	NX_P53384.
PharmGKB	PA31823.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0489.
HOGENOM	HOG000079916.
HOVERGEN	HBG051027.
InParanoid	P53384.
OMA	IFPAKTG.
OrthoDB	EOG4BG8WD.
PhylomeDB	P53384.
Gene expression databases
ArrayExpress	P53384.
Bgee	P53384.
CleanEx	HS_NUBP1.
Genevestigator	P53384.
GermOnline	ENSG00000103274. Homo sapiens.
Family and domain databases
HAMAP	MF_03038. NUBP1.
InterPro	IPR003593. AAA+_ATPase. IPR025723. Anion-transp_ATPase-like_dom. IPR019591. ATPase-like_ParA/MinD. IPR000808. Mrp_CS. [Graphical view]
Pfam	PF02374. ArsA_ATPase. 1 hit. PF10609. ParA. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS01215. MRP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4682.
NextBio	18050.
SOURCE	Search...

Entry information

Entry name

NUBP1_HUMAN

Accession

Primary (citable) accession number: P53384
Secondary accession number(s): Q32M30, Q498A9, Q53FS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 1, 2009
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.