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Protein

Aspartic proteinase MKC7

Gene

MKC7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves proteins C-terminally to the most C-terminal basic residue. Can process the alpha-mating factor precursor. Required for cell wall integrity.3 Publications

Catalytic activityi

Hydrolyzes various precursor proteins with Arg or Lys in P1, and commonly Arg or Lys also in P2. The P3 amino acid is usually non-polar, but otherwise additional basic amino acids are favorable in both non-prime and prime positions.

pH dependencei

Optimum pH is 4-7. Optimum pH is substrate-dependent.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99PROSITE-ProRule annotation1
Active sitei360PROSITE-ProRule annotation1

GO - Molecular functioni

  • aspartic-type endopeptidase activity Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
  • protein catabolic process Source: GO_Central
  • proteolysis Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:G3O-29741-MONOMER.

Protein family/group databases

MEROPSiA01.031.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase MKC7 (EC:3.4.23.41)
Alternative name(s):
Yapsin-2
Gene namesi
Name:MKC7
Synonyms:YPS2
Ordered Locus Names:YDR144C
ORF Names:YD2943.03C, YD8358.01C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR144C.
SGDiS000002551. MKC7.

Subcellular locationi

GO - Cellular componenti

  • anchored component of membrane Source: UniProtKB-KW
  • fungal-type cell wall Source: SGD
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002583523 – 65Sequence analysisAdd BLAST43
ChainiPRO_000002583666 – 575Aspartic proteinase MKC7Add BLAST510
PropeptideiPRO_0000025837576 – 596Removed in mature formSequence analysisAdd BLAST21

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi180N-linked (GlcNAc...)Sequence analysis1
Glycosylationi190N-linked (GlcNAc...)Sequence analysis1
Glycosylationi219N-linked (GlcNAc...)Sequence analysis1
Glycosylationi229N-linked (GlcNAc...)Sequence analysis1
Glycosylationi232N-linked (GlcNAc...)Sequence analysis1
Glycosylationi286N-linked (GlcNAc...)Sequence analysis1
Glycosylationi346N-linked (GlcNAc...)Sequence analysis1
Glycosylationi471N-linked (GlcNAc...)Sequence analysis1
Glycosylationi517N-linked (GlcNAc...)Sequence analysis1
Lipidationi575GPI-anchor amidated asparagineSequence analysis1

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Zymogen

Proteomic databases

MaxQBiP53379.
PRIDEiP53379.

PTM databases

iPTMnetiP53379.

Interactioni

Protein-protein interaction databases

BioGridi32198. 33 interactors.
DIPiDIP-4585N.
IntActiP53379. 1 interactor.
MINTiMINT-515294.

Structurei

3D structure databases

ProteinModelPortaliP53379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 468Peptidase A1PROSITE-ProRule annotationAdd BLAST388

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi532 – 536Poly-Ser5
Compositional biasi567 – 570Poly-Ser4

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00550000075429.
HOGENOMiHOG000248646.
InParanoidiP53379.
KOiK06009.
OMAiYMLFGDN.
OrthoDBiEOG092C3KPP.

Family and domain databases

CDDicd05474. SAP_like. 1 hit.
Gene3Di2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033876. SAP-like.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 3 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53379-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSVLTFVV DALLVCSSIV DAGVTDFPSL PSNEVYVKMN FQKKYGSSFE
60 70 80 90 100
NALDDTKGRT RLMTRDDDYE LVELTNQNSF YSVELDIGTP PQKVTVLVDT
110 120 130 140 150
GSSDLWVTGS DNPYCSTKKK DTTGSSFKQV NKDALASVVE SVFTEISYDT
160 170 180 190 200
TIVTSEATAT FDSTASTSQL IDCATYGTFN TSKSSTFNSN NTEFSIAYGD
210 220 230 240 250
TTFASGTWGH DQLSLNDLNI TGLSFAVANE TNSTVGVLGI GLPGLESTYS
260 270 280 290 300
GVSLSSVQKS YTYNNFPMVL KNSGVIKSTA YSLFANDSDS KHGTILFGAV
310 320 330 340 350
DHGKYAGDLY TIPIINTLQH RGYKDPIQFQ VTLQGLGTSK GDKEDNLTTL
360 370 380 390 400
TTTKIPVLLD SGTTISYMPT ELVKMLADQV GATYSSAYGY YIMDCIKEME
410 420 430 440 450
EESSIIFDFG GFYLSNWLSD FQLVTDSRSN ICILGIAPQS DPTIILGDNF
460 470 480 490 500
LANTYVVYDL DNMEISMAQA NFSDDGEYIE IIESAVPSAL KAPGYSSTWS
510 520 530 540 550
TYESIVSGGN MFSTAANSSI SYFASTSHSA TSSSSSKGQK TQTSTTALSI
560 570 580 590
SKSTSSTSST GMLSPTSSSS TRKENGGHNL NPPFFARFIT AIFHHI
Length:596
Mass (Da):64,269
Last modified:December 21, 2004 - v2
Checksum:iA3C395A370B392BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti546T → A in AAC49112 (PubMed:7479877).Curated1
Sequence conflicti571T → P in AAC49112 (PubMed:7479877).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14733 Genomic DNA. Translation: AAC49112.1.
Z50046 Genomic DNA. Translation: CAA90367.1.
Z54139 Genomic DNA. Translation: CAA90813.1.
BK006938 Genomic DNA. Translation: DAA11987.1.
PIRiS57971.
RefSeqiNP_010428.3. NM_001180451.3.

Genome annotation databases

EnsemblFungiiYDR144C; YDR144C; YDR144C.
GeneIDi851722.
KEGGisce:YDR144C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14733 Genomic DNA. Translation: AAC49112.1.
Z50046 Genomic DNA. Translation: CAA90367.1.
Z54139 Genomic DNA. Translation: CAA90813.1.
BK006938 Genomic DNA. Translation: DAA11987.1.
PIRiS57971.
RefSeqiNP_010428.3. NM_001180451.3.

3D structure databases

ProteinModelPortaliP53379.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32198. 33 interactors.
DIPiDIP-4585N.
IntActiP53379. 1 interactor.
MINTiMINT-515294.

Protein family/group databases

MEROPSiA01.031.

PTM databases

iPTMnetiP53379.

Proteomic databases

MaxQBiP53379.
PRIDEiP53379.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR144C; YDR144C; YDR144C.
GeneIDi851722.
KEGGisce:YDR144C.

Organism-specific databases

EuPathDBiFungiDB:YDR144C.
SGDiS000002551. MKC7.

Phylogenomic databases

GeneTreeiENSGT00550000075429.
HOGENOMiHOG000248646.
InParanoidiP53379.
KOiK06009.
OMAiYMLFGDN.
OrthoDBiEOG092C3KPP.

Enzyme and pathway databases

BioCyciYEAST:G3O-29741-MONOMER.

Miscellaneous databases

PROiP53379.

Family and domain databases

CDDicd05474. SAP_like. 1 hit.
Gene3Di2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033876. SAP-like.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 3 hits.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMKC7_YEAST
AccessioniPrimary (citable) accession number: P53379
Secondary accession number(s): D6VSC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.