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Protein

Tubulin gamma chain

Gene

TUB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays.

Miscellaneous

Present with 7200 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi143 – 149GTPSequence analysis7

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • cytoplasmic microtubule organization Source: InterPro
  • microtubule nucleation Source: SGD
  • mitotic spindle organization in nucleus Source: SGD
  • positive regulation of cytoplasmic translation Source: SGD

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32329-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin gamma chain
Alternative name(s):
Gamma-tubulin
Gene namesi
Name:TUB4
Ordered Locus Names:YLR212C
ORF Names:L8167.21
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR212C
SGDiS000004202 TUB4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000484811 – 473Tubulin gamma chainAdd BLAST473

Proteomic databases

MaxQBiP53378
PaxDbiP53378
PRIDEiP53378

PTM databases

iPTMnetiP53378

Interactioni

Subunit structurei

Interacts with SPC72, SPC97 and SPC98.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31480, 145 interactors
DIPiDIP-821N
IntActiP53378, 14 interactors
MINTiP53378
STRINGi4932.YLR212C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5FLZelectron microscopy6.90C/D1-473[»]
5FM1electron microscopy8.00C/D1-473[»]
ProteinModelPortaliP53378
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000144217
HOGENOMiHOG000165714
InParanoidiP53378
KOiK10389
OMAiEGFMLMH
OrthoDBiEOG092C2U1W

Family and domain databases

CDDicd02188 gamma_tubulin, 1 hit
Gene3Di1.10.287.600, 1 hit
3.30.1330.20, 1 hit
3.40.50.1440, 1 hit
InterProiView protein in InterPro
IPR002454 Gamma_tubulin
IPR008280 Tub_FtsZ_C
IPR000217 Tubulin
IPR018316 Tubulin/FtsZ_2-layer-sand-dom
IPR037103 Tubulin/FtsZ_C_sf
IPR036525 Tubulin/FtsZ_GTPase_sf
IPR023123 Tubulin_C
IPR017975 Tubulin_CS
IPR003008 Tubulin_FtsZ_GTPase
PANTHERiPTHR11588 PTHR11588, 1 hit
PfamiView protein in Pfam
PF00091 Tubulin, 1 hit
PF03953 Tubulin_C, 1 hit
PRINTSiPR01164 GAMMATUBULIN
PR01161 TUBULIN
SMARTiView protein in SMART
SM00864 Tubulin, 1 hit
SM00865 Tubulin_C, 1 hit
SUPFAMiSSF52490 SSF52490, 1 hit
SSF55307 SSF55307, 1 hit
PROSITEiView protein in PROSITE
PS00227 TUBULIN, 1 hit

Sequencei

Sequence statusi: Complete.

P53378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGEIITLQA GQCGNHVGKF LWSQLAKEHA IGTDGLSQLP DSSTERDDDT
60 70 80 90 100
KPFFRENSRN KFTPRAIMMD SEPSVIADVE NTFRGFFDPR NTWVASDGAS
110 120 130 140 150
AGNSWANGYD IGTRNQDDIL NKIDKEIDST DNFEGFQLLH SVAGGTGSGL
160 170 180 190 200
GSNLLEALCD RYPKKILTTY SVFPARSSEV VVQSYNTILA LRRLIEDSDA
210 220 230 240 250
TVVFDNASLL NISGKVFRNP NIDLQHTNQL ISTIISSVTN SIRFPSYMYS
260 270 280 290 300
SMSSIYSTLI PSPELHFLSP SFTPFTSDYI HDDIAHKGHS SYDVMLDLLD
310 320 330 340 350
PSNSLVSTAM NNPTYFNVYN TIIGNVEPRQ ISRAMTKLQQ RIKFPSWSSS
360 370 380 390 400
AMHVNIGRRS PYLPLQPNEN EVSGMMLSNM STVVNVFENA CNTFDKVFAK
410 420 430 440 450
GAFLNNYNVG DLFQSMQNVQ DEFAESREVV QSLMEDYVAA EQDSYLDDVL
460 470
VDDENMVGEL EEDLDADGDH KLV
Length:473
Mass (Da):52,627
Last modified:October 1, 1996 - v1
Checksum:i3EA6F89E4A9A12F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14913 Genomic DNA Translation: AAB67442.1
BK006945 Genomic DNA Translation: DAA09529.1
PIRiS48563
RefSeqiNP_013313.1, NM_001182099.1

Genome annotation databases

EnsemblFungiiYLR212C; YLR212C; YLR212C
GeneIDi850909
KEGGisce:YLR212C

Similar proteinsi

Entry informationi

Entry nameiTBG_YEAST
AccessioniPrimary (citable) accession number: P53378
Secondary accession number(s): D6VYL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 25, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health