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Protein

Nucleoside diphosphate-linked moiety X motif 6

Gene

NUDT6

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May contribute to the regulation of cell proliferation.1 Publication

GO - Molecular functioni

  • growth factor activity Source: UniProtKB
  • hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside diphosphate-linked moiety X motif 6 (EC:3.6.1.-)
Short name:
Nudix motif 6
Alternative name(s):
Antisense basic fibroblast growth factor
Protein GFG
Gene namesi
Name:NUDT6
Synonyms:FGF2AS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

HGNCiHGNC:8053. NUDT6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31839.

Polymorphism and mutation databases

BioMutaiNUDT6.
DMDMi17380446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Nucleoside diphosphate-linked moiety X motif 6PRO_0000057107Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki76 – 76Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP53370.
MaxQBiP53370.
PaxDbiP53370.
PRIDEiP53370.

PTM databases

iPTMnetiP53370.
PhosphoSiteiP53370.

Expressioni

Tissue specificityi

Detected in liver, kidney and esophagus (at protein level). Ubiquitous.2 Publications

Gene expression databases

BgeeiP53370.
CleanExiHS_NUDT6.
ExpressionAtlasiP53370. baseline and differential.
GenevisibleiP53370. HS.

Organism-specific databases

HPAiHPA039202.

Interactioni

Subunit structurei

Monomer and homodimer.1 Publication

GO - Molecular functioni

  • growth factor activity Source: UniProtKB

Protein-protein interaction databases

BioGridi116333. 11 interactions.
IntActiP53370. 4 interactions.
STRINGi9606.ENSP00000306070.

Structurei

Secondary structure

1
316
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 594Combined sources
Helixi60 – 634Combined sources
Turni64 – 663Combined sources
Helixi71 – 8717Combined sources
Beta strandi92 – 987Combined sources
Helixi99 – 1046Combined sources
Helixi105 – 1106Combined sources
Beta strandi114 – 1207Combined sources
Beta strandi122 – 1287Combined sources
Beta strandi145 – 1528Combined sources
Turni153 – 1564Combined sources
Beta strandi157 – 16711Combined sources
Beta strandi170 – 1723Combined sources
Beta strandi175 – 1773Combined sources
Helixi184 – 19613Combined sources
Beta strandi200 – 21011Combined sources
Beta strandi223 – 23210Combined sources
Turni240 – 2423Combined sources
Beta strandi243 – 2508Combined sources
Helixi251 – 2566Combined sources
Beta strandi258 – 2603Combined sources
Helixi262 – 27716Combined sources
Helixi279 – 2813Combined sources
Beta strandi284 – 2907Combined sources
Beta strandi292 – 2954Combined sources
Beta strandi297 – 3037Combined sources
Helixi307 – 3104Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FXTX-ray2.30A/B/C/D/E/F/G/H45-134[»]
3H95X-ray1.70A141-316[»]
ProteinModelPortaliP53370.
SMRiP53370. Positions 45-131, 143-312.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53370.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini141 – 273133Nudix hydrolasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi176 – 19722Nudix boxAdd
BLAST

Sequence similaritiesi

Belongs to the Nudix hydrolase family.Curated
Contains 1 nudix hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0648. Eukaryota.
ENOG410Z4GH. LUCA.
GeneTreeiENSGT00390000008458.
HOGENOMiHOG000231274.
HOVERGENiHBG052692.
InParanoidiP53370.
OMAiQAAVQQW.
OrthoDBiEOG7VB2GP.
PhylomeDBiP53370.
TreeFamiTF106346.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR003293. Nudix_hydrolase6-like.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR01356. GFGPROTEIN.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P53370-1) [UniParc]FASTAAdd to basket

Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRQPLSWGRW RAMLARTYGP GPSAGYRWAS GAQGYVRNPP VGACDLQGEL
60 70 80 90 100
DRFGGISVRL ARLDALDRLD AAAFQKGLQA AVQQWRSEGR TAVWLHIPIL
110 120 130 140 150
QSRFIAPAAS LGFCFHHAES DSSTLTLWLR EGPSRLPGYA SHQVGVAGAV
160 170 180 190 200
FDESTRKILV VQDRNKLKNM WKFPGGLSEP EEDIGDTAVR EVFEETGIKS
210 220 230 240 250
EFRSVLSIRQ QHTNPGAFGK SDMYIICRLK PYSFTINFCQ EECLRCEWMD
260 270 280 290 300
LNDLAKTENT TPITSRVARL LLYGYREGFD KIDLTVEELP AVYTGLFYKL
310
YHKELPENYK TMKGID
Length:316
Mass (Da):35,679
Last modified:November 16, 2001 - v2
Checksum:i41EC95893B52180A
GO
Isoform 2 (identifier: P53370-2) [UniParc]FASTAAdd to basket

Also known as: A

The sequence of this isoform differs from the canonical sequence as follows:
     1-169: Missing.

Show »
Length:147
Mass (Da):17,082
Checksum:iA74B7E75EA979B4D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti181 – 1811E → G in AAA67062 (PubMed:7984147).Curated
Sequence conflicti198 – 1981I → V in AAA67062 (PubMed:7984147).Curated
Sequence conflicti205 – 2051V → L in AAA67062 (PubMed:7984147).Curated
Sequence conflicti213 – 2142TN → RS in AAA67062 (PubMed:7984147).Curated
Sequence conflicti217 – 2171A → R in AAA67062 (PubMed:7984147).Curated
Sequence conflicti220 – 2201K → M in AAA67062 (PubMed:7984147).Curated
Sequence conflicti225 – 2251I → L in AAA67062 (PubMed:7984147).Curated
Sequence conflicti230 – 2323KPY → QPR in AAA67062 (PubMed:7984147).Curated
Sequence conflicti241 – 2411E → Q in AAA67062 (PubMed:7984147).Curated
Sequence conflicti245 – 2451R → K in AAA67062 (PubMed:7984147).Curated
Sequence conflicti252 – 2532ND → ES in AAA67062 (PubMed:7984147).Curated
Sequence conflicti256 – 2594KTEN → RTKH in AAA67062 (PubMed:7984147).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti114 – 1141C → R.
Corresponds to variant rs12648093 [ dbSNP | Ensembl ].
VAR_050412
Natural varianti209 – 2091R → Q.
Corresponds to variant rs1048201 [ dbSNP | Ensembl ].
VAR_021909

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 169169Missing in isoform 2. CuratedVSP_003729Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019633 mRNA. Translation: AAD01636.2.
AF019632 mRNA. Translation: AAD01635.1.
AK291871 mRNA. Translation: BAF84560.1.
BC009842 mRNA. Translation: AAH09842.1.
L31408 mRNA. Translation: AAA67062.1.
CCDSiCCDS3729.1. [P53370-2]
CCDS43268.1. [P53370-1]
RefSeqiNP_009014.2. NM_007083.4. [P53370-1]
NP_932158.1. NM_198041.2. [P53370-2]
UniGeneiHs.558459.

Genome annotation databases

EnsembliENST00000304430; ENSP00000306070; ENSG00000170917. [P53370-1]
ENST00000339154; ENSP00000344011; ENSG00000170917. [P53370-2]
ENST00000502270; ENSP00000424117; ENSG00000170917. [P53370-2]
GeneIDi11162.
KEGGihsa:11162.
UCSCiuc003iew.3. human. [P53370-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF019633 mRNA. Translation: AAD01636.2.
AF019632 mRNA. Translation: AAD01635.1.
AK291871 mRNA. Translation: BAF84560.1.
BC009842 mRNA. Translation: AAH09842.1.
L31408 mRNA. Translation: AAA67062.1.
CCDSiCCDS3729.1. [P53370-2]
CCDS43268.1. [P53370-1]
RefSeqiNP_009014.2. NM_007083.4. [P53370-1]
NP_932158.1. NM_198041.2. [P53370-2]
UniGeneiHs.558459.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FXTX-ray2.30A/B/C/D/E/F/G/H45-134[»]
3H95X-ray1.70A141-316[»]
ProteinModelPortaliP53370.
SMRiP53370. Positions 45-131, 143-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116333. 11 interactions.
IntActiP53370. 4 interactions.
STRINGi9606.ENSP00000306070.

PTM databases

iPTMnetiP53370.
PhosphoSiteiP53370.

Polymorphism and mutation databases

BioMutaiNUDT6.
DMDMi17380446.

Proteomic databases

EPDiP53370.
MaxQBiP53370.
PaxDbiP53370.
PRIDEiP53370.

Protocols and materials databases

DNASUi11162.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304430; ENSP00000306070; ENSG00000170917. [P53370-1]
ENST00000339154; ENSP00000344011; ENSG00000170917. [P53370-2]
ENST00000502270; ENSP00000424117; ENSG00000170917. [P53370-2]
GeneIDi11162.
KEGGihsa:11162.
UCSCiuc003iew.3. human. [P53370-1]

Organism-specific databases

CTDi11162.
GeneCardsiNUDT6.
HGNCiHGNC:8053. NUDT6.
HPAiHPA039202.
MIMi606261. gene.
neXtProtiNX_P53370.
PharmGKBiPA31839.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0648. Eukaryota.
ENOG410Z4GH. LUCA.
GeneTreeiENSGT00390000008458.
HOGENOMiHOG000231274.
HOVERGENiHBG052692.
InParanoidiP53370.
OMAiQAAVQQW.
OrthoDBiEOG7VB2GP.
PhylomeDBiP53370.
TreeFamiTF106346.

Miscellaneous databases

EvolutionaryTraceiP53370.
GeneWikiiNUDT6.
GenomeRNAii11162.
PROiP53370.
SOURCEiSearch...

Gene expression databases

BgeeiP53370.
CleanExiHS_NUDT6.
ExpressionAtlasiP53370. baseline and differential.
GenevisibleiP53370. HS.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR003293. Nudix_hydrolase6-like.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF00293. NUDIX. 1 hit.
[Graphical view]
PRINTSiPR01356. GFGPROTEIN.
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the promoter for the human antisense fibroblast growth factor-2 gene; regulation by Ets in Jurkat T cells."
    Gagnon M.L., Moy G.K., Klagsbrun M.
    J. Cell. Biochem. 72:492-506(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  4. "Identification and characterization of an antisense RNA transcript (gfg) from the human basic fibroblast growth factor gene."
    Murphy P.R., Knee R.S.
    Mol. Endocrinol. 8:852-859(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 170-269.
    Tissue: Astrocytoma.
  5. "The endogenous fibroblast growth factor-2 antisense gene product regulates pituitary cell growth and hormone production."
    Asa S.L., Ramyar L., Murphy P.R., Li A.W., Ezzat S.
    Mol. Endocrinol. 15:589-599(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Alternative splicing of the FGF antisense gene: differential subcellular localization in human tissues and esophageal adenocarcinoma."
    Zhang S.C., Barclay C., Alexander L.A., Geldenhuys L., Porter G.A., Casson A.G., Murphy P.R.
    J. Mol. Med. 85:1215-1228(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ALTERNATIVE SPLICING, ABSENCE OF ANTI-MUTATOR FUNCTION IN DNA REPAIR, SUBCELLULAR LOCATION, SUBUNIT, TISSUE SPECIFICITY.
  7. "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells."
    Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D.
    J. Proteome Res. 6:298-305(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-76.
    Tissue: Lung adenocarcinoma.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. "Crystal structure of the N-terminal domain and of nudix domain of human NUDT6."
    Structural genomics consortium (SGC)
    Submitted (JUL-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 45-316.

Entry informationi

Entry nameiNUDT6_HUMAN
AccessioniPrimary (citable) accession number: P53370
Secondary accession number(s): A8K756, O95097, Q9UQD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: November 16, 2001
Last modified: June 8, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein is coded from a FGF2 (BFGF) gene antisense transcript.

Caution

The rat protein was reported to play a role in DNA repair (PubMed:9406864), based on its ability to complement E.coli deficient in the DNA repair enzyme mutT that hydrolyzes oxidized guanine nucleotides. PubMed:17569023 found no such activity, neither for the human nor the rat protein.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.