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Protein

7,8-dihydro-8-oxoguanine triphosphatase

Gene

Nudt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Antimutagenic. Plays a redundant role in sanitizing oxidized nucleotide pools, such as 8-oxo-dGTP pools. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress (PubMed:7586133). Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP (By similarity). Does not play a role in U8 snoRNA decapping activity. Binds U8 snoRNA (By similarity).By similarity1 Publication

Catalytic activityi

8-oxo-dGTP + H2O = 8-oxo-dGMP + diphosphate.1 Publication
2-hydroxy-dATP + H2O = 2-hydroxy-dAMP + diphosphate.By similarity

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8Substrate; via carbonyl oxygenBy similarity1
Binding sitei23SubstrateBy similarity1
Sitei27Important for 2-OH-dATPase and 8-oxo-dGTPase activitiesBy similarity1
Binding sitei33SubstrateBy similarity1
Metal bindingi37Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi52MagnesiumBy similarity1
Metal bindingi55MagnesiumBy similarity1
Metal bindingi56MagnesiumBy similarity1
Sitei117Essential for 2-OH-dATPase and 8-oxo-dGTPase activitiesBy similarity1
Sitei119Essential for 2-OH-dATPase activity and important for 8-oxo-dGTPase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aging Source: RGD
  • dATP catabolic process Source: UniProtKB
  • dGTP catabolic process Source: UniProtKB
  • DNA protection Source: UniProtKB
  • DNA repair Source: InterPro
  • male gonad development Source: RGD
  • purine nucleotide catabolic process Source: UniProtKB
  • response to cadmium ion Source: RGD

Keywordsi

Molecular functionHydrolase, RNA-binding
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2393930 Phosphate bond hydrolysis by NUDT proteins

Names & Taxonomyi

Protein namesi
Recommended name:
7,8-dihydro-8-oxoguanine triphosphatase (EC:3.6.1.551 Publication)
Alternative name(s):
2-hydroxy-dATP diphosphatase (EC:3.6.1.56By similarity)
8-oxo-dGTPase
Nucleoside diphosphate-linked moiety X motif 1
Short name:
Nudix motif 1
Gene namesi
Name:Nudt1
Synonyms:Mth1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi621080 Nudt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000005710121 – 1567,8-dihydro-8-oxoguanine triphosphataseAdd BLAST136

Proteomic databases

PaxDbiP53369

PTM databases

PhosphoSitePlusiP53369

Expressioni

Tissue specificityi

Expressed in liver in hepatocytes and weakly in Kupffer's cells. Expression detected in testes in primary and secondary spermatocytes and interstitial cells. High expression levels detected in the inner cortex of kidney, with lower levels detected in the tubules of the outer cortex, medulla, renal pelvis and glomeruli (at protein level). Expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.2 Publications

Gene expression databases

BgeeiENSRNOG00000001260
GenevisibleiP53369 RN

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001700

Structurei

3D structure databases

ProteinModelPortaliP53369
SMRiP53369
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 132Nudix hydrolasePROSITE-ProRule annotationAdd BLAST112

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 38Substrate bindingBy similarity4
Regioni117 – 120Substrate bindingBy similarity4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 58Nudix boxPROSITE-ProRule annotationAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IXIA Eukaryota
COG0494 LUCA
GeneTreeiENSGT00390000000341
HOGENOMiHOG000261970
HOVERGENiHBG000032
InParanoidiP53369
KOiK17816
OMAiGAGKWNG
OrthoDBiEOG091G0O4Y
PhylomeDBiP53369
TreeFamiTF106348

Family and domain databases

InterProiView protein in InterPro
IPR020476 Nudix_hydrolase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
IPR003563 OxG-triPHTase
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
PRINTSiPR01403 8OXTPHPHTASE
PR00502 NUDIXFAMILY
SUPFAMiSSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSRLYTLV LVLQPQRVLL GMKKRGFGAG RWNGFGGKVQ EGETIEDGAK
60 70 80 90 100
RELLEESGLR VDTLHKVGHI SFEFVGSPEL MDVHIFSTDH VHGTPTESEE
110 120 130 140 150
MRPQWFQLDQ IPFADMWPDD SYWFPLLLQK KKFCGHFKFH GQDTILSYSL

REVDEF
Length:156
Mass (Da):18,018
Last modified:October 1, 1996 - v1
Checksum:i60AFB6522CB03E18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D49977 mRNA Translation: BAA08726.1
D49980 Genomic DNA Translation: BAA08727.1
RefSeqiNP_476461.1, NM_057120.1
XP_006248973.1, XM_006248911.3
UniGeneiRn.10669

Genome annotation databases

EnsembliENSRNOT00000001700; ENSRNOP00000001700; ENSRNOG00000001260
GeneIDi117260
KEGGirno:117260
UCSCiRGD:621080 rat

Entry informationi

Entry namei8ODP_RAT
AccessioniPrimary (citable) accession number: P53369
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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