P53367 (ARFP1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arfaptin-1

Alternative name(s):
ADP-ribosylation factor-interacting protein 1

Gene names

Name:

ARFIP1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative target protein of ADP-ribosylation factor.
Subunit structure	Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6. Ref.4
Tissue specificity	Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.
Sequence similarities	Contains 1 AH domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from direct assay. Source: MGI regulation of protein secretion Inferred from direct assay. Source: MGI
Cellular component	Golgi membrane Inferred from direct assay Ref.1. Source: UniProtKB cytosol Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P53367-1)

Also known as: 1b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P53367-2)

Also known as: 1a;

The sequence of this isoform differs from the canonical sequence as follows:
     69-100: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Arfaptin-1		PRO_0000064665
Regions
	Domain	153 – 353	201	AH
Amino acid modifications
	Modified residue	69	1	Phosphoserine Ref.5
	Modified residue	79	1	Phosphoserine Ref.5
	Modified residue	132	1	Phosphoserine Ref.5 Ref.6
	Modified residue	272	1	Phosphothreonine Ref.7
	Modified residue	361	1	Phosphothreonine Ref.7
Natural variations
	Alternative sequence	69 – 100	32	Missing in isoform A.		VSP_004088

Sequences

	Sequence		Length	Mass (Da)
	Isoform B (1b) [UniParc]. Last modified May 27, 2002. Version 2. Checksum: 7A315D0E67DECDBF	FASTA	373	41,738
	10 20 30 40 50 60 MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS HGFDNTKEGV 70 80 90 100 110 120 IEAGAFQGSP APPLPSVMSP SRVAASRLAQ QGSDLIVPAG GQRTQTKSGP VILADEIKNP 130 140 150 160 170 180 AMEKLELVRK WSLNTYKCTR QIISEKLGRG SRTVDLELEA QIDILRDNKK KYENILKLAQ 190 200 210 220 230 240 TLSTQLFQMV HTQRQLGDAF ADLSLKSLEL HEEFGYNADT QKLLAKNGET LLGAINFFIA 250 260 270 280 290 300 SVNTLVNKTI EDTLMTVKQY ESARIEYDAY RTDLEELNLG PRDANTLPKI EQSQHLFQAH 310 320 330 340 350 360 KEKYDKMRND VSVKLKFLEE NKVKVLHNQL VLFHNAIAAY FAGNQKQLEQ TLKQFHIKLK 370 TPGVDAPSWL EEQ
	Isoform A (1a) [UniParc]. Checksum: 94675E3FF3FB9D08	FASTA	341	38,599
	10 20 30 40 50 60 MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS HGFDNTKEGV 70 80 90 100 110 120 IEAGAFQGGQ RTQTKSGPVI LADEIKNPAM EKLELVRKWS LNTYKCTRQI ISEKLGRGSR 130 140 150 160 170 180 TVDLELEAQI DILRDNKKKY ENILKLAQTL STQLFQMVHT QRQLGDAFAD LSLKSLELHE 190 200 210 220 230 240 EFGYNADTQK LLAKNGETLL GAINFFIASV NTLVNKTIED TLMTVKQYES ARIEYDAYRT 250 260 270 280 290 300 DLEELNLGPR DANTLPKIEQ SQHLFQAHKE KYDKMRNDVS VKLKFLEENK VKVLHNQLVL 310 320 330 340 FHNAIAAYFA GNQKQLEQTL KQFHIKLKTP GVDAPSWLEE Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes." Kanoh H., Williger B.-T., Exton J.H. J. Biol. Chem. 272:5421-5429(1997) [PubMed: 9038142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
[2]	"Arfaptin-1b, a long splice variant of arfaptin-1." Premont R.T., Lefkowitz R.J. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B). Tissue: Uterus.
[4]	"Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D." Williger B.-T., Provost J.J., Ho W.-T., Milstine J., Exton J.H. FEBS Lett. 454:85-89(1999) [PubMed: 10413101] [Abstract] Cited for: INTERACTION WITH ARF1; ARF3; ARF5 AND ARF6.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69; SER-79 AND SER-132, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[6]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-272 AND THR-361, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U52521 mRNA. Translation: AAA97923.1. AF124489 mRNA. Translation: AAD29390.1. BC103759 mRNA. Translation: AAI03760.1.
IPI	IPI00021258. IPI00216520.
PIR	G02515.
RefSeq	NP_001020764.1. NM_001025593.1. NP_001020766.1. NM_001025595.1. NP_055262.1. NM_014447.2.
UniGene	Hs.416089.
3D structure databases
ProteinModelPortal	P53367.
SMR	P53367. Positions 151-351.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53367. 1 interaction.
MINT	MINT-3019815.
STRING	P53367.
PTM databases
PhosphoSite	P53367.
Polymorphism databases
DMDM	21264399.
Proteomic databases
PRIDE	P53367.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000353617; ENSP00000296557; ENSG00000164144. ENST00000405727; ENSP00000384189; ENSG00000164144.
GeneID	27236.
KEGG	hsa:27236.
UCSC	uc003imz.1. human.
Organism-specific databases
CTD	27236.
GeneCards	GC04P153701.
H-InvDB	HIX0004575.
HGNC	HGNC:21496. ARFIP1.
HPA	CAB005080.
MIM	605928. gene.
neXtProt	NX_P53367.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11170.
GeneTree	ENSGT00500000044828.
HOGENOM	HBG714438.
HOVERGEN	HBG050564.
InParanoid	P53367.
OMA	TKNPAME.
OrthoDB	EOG4BCDNH.
PhylomeDB	P53367.
Gene expression databases
ArrayExpress	P53367.
Bgee	P53367.
CleanEx	HS_ARFIP1.
Genevestigator	P53367.
GermOnline	ENSG00000164144. Homo sapiens.
Family and domain databases
InterPro	IPR010504. Arfaptin_homology_dom. [Graphical view]
Gene3D	G3DSA:1.20.1270.60. Arfaptin. 1 hit.
Pfam	PF06456. Arfaptin. 1 hit. [Graphical view]
SMART	SM01015. Arfaptin. 1 hit. [Graphical view]
PROSITE	PS50870. AH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	50103.
SOURCE	Search...

Entry information

Entry name

ARFP1_HUMAN

Accession

Primary (citable) accession number: P53367
Secondary accession number(s): Q3SYL4, Q9Y2X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.