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P53367

- ARFP1_HUMAN

UniProt

P53367 - ARFP1_HUMAN

Protein

Arfaptin-1

Gene

ARFIP1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    Putative target protein of ADP-ribosylation factor.

    GO - Biological processi

    1. intracellular protein transport Source: MGI
    2. regulation of protein secretion Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arfaptin-1
    Alternative name(s):
    ADP-ribosylation factor-interacting protein 1
    Gene namesi
    Name:ARFIP1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 4

    Organism-specific databases

    HGNCiHGNC:21496. ARFIP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MGI
    2. Golgi membrane Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134920621.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 373372Arfaptin-1PRO_0000064665Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine3 Publications
    Modified residuei5 – 51Phosphoserine1 Publication
    Modified residuei69 – 691Phosphoserine2 Publications
    Modified residuei79 – 791Phosphoserine1 Publication
    Modified residuei132 – 1321Phosphoserine2 Publications
    Modified residuei361 – 3611Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP53367.
    PaxDbiP53367.
    PRIDEiP53367.

    PTM databases

    PhosphoSiteiP53367.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart.

    Gene expression databases

    ArrayExpressiP53367.
    BgeeiP53367.
    CleanExiHS_ARFIP1.
    GenevestigatoriP53367.

    Organism-specific databases

    HPAiCAB005080.
    HPA037375.

    Interactioni

    Subunit structurei

    Interacts with non-myristoylated GTP-bound ARF3, but not to GDP-bound ARF3, and also to ARF1. Binds with lower affinity to ARF5 and with very little affinity to ARF6.1 Publication

    Protein-protein interaction databases

    BioGridi118084. 19 interactions.
    IntActiP53367. 3 interactions.
    MINTiMINT-3019815.
    STRINGi9606.ENSP00000296557.

    Structurei

    3D structure databases

    ProteinModelPortaliP53367.
    SMRiP53367. Positions 151-351.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini153 – 353201AHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG302946.
    HOGENOMiHOG000236308.
    HOVERGENiHBG050564.
    InParanoidiP53367.
    OMAiLLTVKQY.
    OrthoDBiEOG7VQJD9.
    PhylomeDBiP53367.
    TreeFamiTF314945.

    Family and domain databases

    Gene3Di1.20.1270.60. 1 hit.
    InterProiIPR027267. AH/BAR-dom.
    IPR010504. AH_dom.
    [Graphical view]
    PfamiPF06456. Arfaptin. 1 hit.
    [Graphical view]
    SMARTiSM01015. Arfaptin. 1 hit.
    [Graphical view]
    PROSITEiPS50870. AH. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform B (identifier: P53367-1) [UniParc]FASTAAdd to Basket

    Also known as: 1b

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQESPKNSA AEIPVTSNGE VDDSREHSFN RDLKHSLPSG LGLSETQITS    50
    HGFDNTKEGV IEAGAFQGSP APPLPSVMSP SRVAASRLAQ QGSDLIVPAG 100
    GQRTQTKSGP VILADEIKNP AMEKLELVRK WSLNTYKCTR QIISEKLGRG 150
    SRTVDLELEA QIDILRDNKK KYENILKLAQ TLSTQLFQMV HTQRQLGDAF 200
    ADLSLKSLEL HEEFGYNADT QKLLAKNGET LLGAINFFIA SVNTLVNKTI 250
    EDTLMTVKQY ESARIEYDAY RTDLEELNLG PRDANTLPKI EQSQHLFQAH 300
    KEKYDKMRND VSVKLKFLEE NKVKVLHNQL VLFHNAIAAY FAGNQKQLEQ 350
    TLKQFHIKLK TPGVDAPSWL EEQ 373
    Length:373
    Mass (Da):41,738
    Last modified:May 27, 2002 - v2
    Checksum:i7A315D0E67DECDBF
    GO
    Isoform A (identifier: P53367-2) [UniParc]FASTAAdd to Basket

    Also known as: 1a

    The sequence of this isoform differs from the canonical sequence as follows:
         69-100: Missing.

    Show »
    Length:341
    Mass (Da):38,599
    Checksum:i94675E3FF3FB9D08
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei69 – 10032Missing in isoform A. 3 PublicationsVSP_004088Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52521 mRNA. Translation: AAA97923.1.
    AF124489 mRNA. Translation: AAD29390.1.
    AK290472 mRNA. Translation: BAF83161.1.
    CH471056 Genomic DNA. Translation: EAX04968.1.
    CH471056 Genomic DNA. Translation: EAX04969.1.
    BC103759 mRNA. Translation: AAI03760.1.
    BC105114 mRNA. Translation: AAI05115.1.
    BC105116 mRNA. Translation: AAI05117.1.
    BC143675 mRNA. Translation: AAI43676.1.
    CCDSiCCDS34080.1. [P53367-1]
    CCDS3780.1. [P53367-2]
    PIRiG02515.
    RefSeqiNP_001020764.1. NM_001025593.2. [P53367-2]
    NP_001020766.1. NM_001025595.2. [P53367-1]
    NP_001274360.1. NM_001287431.1. [P53367-1]
    NP_001274361.1. NM_001287432.1. [P53367-1]
    NP_001274362.1. NM_001287433.1. [P53367-2]
    NP_055262.1. NM_014447.3. [P53367-2]
    UniGeneiHs.416089.

    Genome annotation databases

    EnsembliENST00000353617; ENSP00000296557; ENSG00000164144. [P53367-1]
    ENST00000356064; ENSP00000348360; ENSG00000164144. [P53367-2]
    ENST00000405727; ENSP00000384189; ENSG00000164144. [P53367-2]
    ENST00000451320; ENSP00000395083; ENSG00000164144. [P53367-1]
    GeneIDi27236.
    KEGGihsa:27236.
    UCSCiuc003imz.3. human. [P53367-1]
    uc003ina.3. human. [P53367-2]

    Polymorphism databases

    DMDMi21264399.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52521 mRNA. Translation: AAA97923.1 .
    AF124489 mRNA. Translation: AAD29390.1 .
    AK290472 mRNA. Translation: BAF83161.1 .
    CH471056 Genomic DNA. Translation: EAX04968.1 .
    CH471056 Genomic DNA. Translation: EAX04969.1 .
    BC103759 mRNA. Translation: AAI03760.1 .
    BC105114 mRNA. Translation: AAI05115.1 .
    BC105116 mRNA. Translation: AAI05117.1 .
    BC143675 mRNA. Translation: AAI43676.1 .
    CCDSi CCDS34080.1. [P53367-1 ]
    CCDS3780.1. [P53367-2 ]
    PIRi G02515.
    RefSeqi NP_001020764.1. NM_001025593.2. [P53367-2 ]
    NP_001020766.1. NM_001025595.2. [P53367-1 ]
    NP_001274360.1. NM_001287431.1. [P53367-1 ]
    NP_001274361.1. NM_001287432.1. [P53367-1 ]
    NP_001274362.1. NM_001287433.1. [P53367-2 ]
    NP_055262.1. NM_014447.3. [P53367-2 ]
    UniGenei Hs.416089.

    3D structure databases

    ProteinModelPortali P53367.
    SMRi P53367. Positions 151-351.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 118084. 19 interactions.
    IntActi P53367. 3 interactions.
    MINTi MINT-3019815.
    STRINGi 9606.ENSP00000296557.

    PTM databases

    PhosphoSitei P53367.

    Polymorphism databases

    DMDMi 21264399.

    Proteomic databases

    MaxQBi P53367.
    PaxDbi P53367.
    PRIDEi P53367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000353617 ; ENSP00000296557 ; ENSG00000164144 . [P53367-1 ]
    ENST00000356064 ; ENSP00000348360 ; ENSG00000164144 . [P53367-2 ]
    ENST00000405727 ; ENSP00000384189 ; ENSG00000164144 . [P53367-2 ]
    ENST00000451320 ; ENSP00000395083 ; ENSG00000164144 . [P53367-1 ]
    GeneIDi 27236.
    KEGGi hsa:27236.
    UCSCi uc003imz.3. human. [P53367-1 ]
    uc003ina.3. human. [P53367-2 ]

    Organism-specific databases

    CTDi 27236.
    GeneCardsi GC04P153701.
    HGNCi HGNC:21496. ARFIP1.
    HPAi CAB005080.
    HPA037375.
    MIMi 605928. gene.
    neXtProti NX_P53367.
    PharmGKBi PA134920621.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG302946.
    HOGENOMi HOG000236308.
    HOVERGENi HBG050564.
    InParanoidi P53367.
    OMAi LLTVKQY.
    OrthoDBi EOG7VQJD9.
    PhylomeDBi P53367.
    TreeFami TF314945.

    Miscellaneous databases

    ChiTaRSi ARFIP1. human.
    GeneWikii ARFIP1.
    GenomeRNAii 27236.
    NextBioi 50103.
    PROi P53367.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53367.
    Bgeei P53367.
    CleanExi HS_ARFIP1.
    Genevestigatori P53367.

    Family and domain databases

    Gene3Di 1.20.1270.60. 1 hit.
    InterProi IPR027267. AH/BAR-dom.
    IPR010504. AH_dom.
    [Graphical view ]
    Pfami PF06456. Arfaptin. 1 hit.
    [Graphical view ]
    SMARTi SM01015. Arfaptin. 1 hit.
    [Graphical view ]
    PROSITEi PS50870. AH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes."
      Kanoh H., Williger B.-T., Exton J.H.
      J. Biol. Chem. 272:5421-5429(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    2. "Arfaptin-1b, a long splice variant of arfaptin-1."
      Premont R.T., Lefkowitz R.J.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
      Tissue: Liver.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Tissue: Brain.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
      Tissue: Brain and Uterus.
    6. "Arfaptin 1 forms a complex with ADP-ribosylation factor and inhibits phospholipase D."
      Williger B.-T., Provost J.J., Ho W.-T., Milstine J., Exton J.H.
      FEBS Lett. 454:85-89(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARF1; ARF3; ARF5 AND ARF6.
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-69 AND SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-361, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-5; SER-69 AND SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARFP1_HUMAN
    AccessioniPrimary (citable) accession number: P53367
    Secondary accession number(s): Q2M2X4, Q3SYL4, Q9Y2X6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 4
      Human chromosome 4: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3