Reviewed, UniProtKB/Swiss-Prot P53365 (ARFP2_HUMAN)

Names and origin

Protein names

Recommended name:
    Arfaptin-2
Alternative name(s):
    ADP-ribosylation factor-interacting protein 2
    Partner of RAC1
      Short name=Protein POR1

Gene names

Name:	ARFIP2
Synonyms:	POR1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
Subunit structure	Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities.
Sequence similarities	Contains 1 AH domain.

Ontologies

Keywords
Technical term	3D-structure
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from mutant phenotype. Source: UniProtKB cell motion Ref.3 Traceable author statement. Source: ProtInc lamellipodium biogenesis Traceable author statement. Source: UniProtKB ruffle organization Traceable author statement. Source: UniProtKB small GTPase mediated signal transduction Ref.3 Traceable author statement. Source: ProtInc
Cellular component	cell cortex Inferred from direct assay. Source: UniProtKB plasma membrane Traceable author statement. Source: UniProtKB ruffle Ref.3 Inferred from mutant phenotype. Source: UniProtKB
Molecular function	GTP binding Ref.3 Traceable author statement. Source: ProtInc GTP-dependent protein binding Inferred from physical interaction. Source: UniProtKB Rac GTPase binding Traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARF6	P62330	1	EBI-638194,EBI-638181
RAC1	P63000	4	EBI-638194,EBI-413628

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 341

341

Arfaptin-2

PRO_0000064667

Regions

Domain

121 – 321

201

AH

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

341

Helix Strand Turn

Details...

Helix

124 – 174

51

Turn

176 – 178

3

Helix

179 – 215

37

Helix

217 – 245

29

Helix

254 – 316

63

Sequences

	Sequence		Length	Mass (Da)
	P53365-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AB8D224430D8E213	FASTA	341	37,856
	10 20 30 40 50 60 MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI VSGGYGGSGD 70 80 90 100 110 120 GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG INTYKCTKQL LSERFGRGSR 130 140 150 160 170 180 TVDLELELQI ELLRETKRKY ESVLQLGRAL TAHLYSLLQT QHALGDAFAD LSQKSPELQE 190 200 210 220 230 240 EFGYNAETQK LLCKNGETLL GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT 250 260 270 280 290 300 DLEELSLGPR DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL 310 320 330 340 FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes." Kanoh H., Williger B.-T., Exton J.H. J. Biol. Chem. 272:5421-5429(1997) [PubMed: 9038142] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Identification of a novel Rac1-interacting protein involved in membrane ruffling." van Aelst L., Joneson T., Bar-Sagi D. EMBO J. 15:3778-3786(1996) [PubMed: 8670882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-341.
[4]	"The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways." Tarricone C., Xiao B., Justin N., Walker P.A., Rittinger K., Gamblin S.J., Smerdon S.J. Nature 411:215-219(2001) [PubMed: 11346801] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 118-341 IN COMPLEX WITH RAC1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U52522 mRNA. Translation: AAA97924.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.

PIR

G02516.

RefSeq

NP_036534.1.

UniGene

Hs.75139

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I49	X-ray	2.80	A/B	118-341	[»]
1I4D	X-ray	2.50	A/B	118-341	[»]
1I4L	X-ray	2.70	A/B	118-341	[»]
1I4T	X-ray	2.60	A/B	118-341	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P53365.

PTM databases

PhosphoSite

P53365.

Genome annotation databases

Ensembl

ENSG00000132254. Homo sapiens. [Contig view]

GeneID

23647.

KEGG

hsa:23647.

Organism-specific databases

H-InvDB

HIX0022270.

HGNC

HGNC:17160. ARFIP2.

MIM

601638. gene.

PharmGKB

PA134879398.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P53365.

HOVERGEN

P53365.

Gene expression databases

ArrayExpress

P53365.

CleanEx

HS_ARFIP2.

GermOnline

ENSG00000132254. Homo sapiens.

Family and domain databases

InterPro

IPR010504. Arfaptin.
[Graphical view]

Gene3D

G3DSA:1.20.1270.60. Arfaptin. 1 hit.

Pfam

PF06456. Arfaptin. 1 hit.
[Graphical view]

PROSITE

PS50870. AH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

LinkHub

P53365.

NextBio

46467.

SOURCE

Search...

Entry information

Entry name

ARFP2_HUMAN

Accession

Primary (citable) accession number: P53365

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)