P53365 (ARFP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arfaptin-2

Alternative name(s):
ADP-ribosylation factor-interacting protein 2
Partner of RAC1
Short name=Protein POR1

Gene names

Name:	ARFIP2
Synonyms:	POR1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.
Subunit structure	Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level. Ref.7
Sequence similarities	Contains 1 AH domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton organization Inferred from mutant phenotype PubMed 9312003. Source: UniProtKB cellular component movement Traceable author statement Ref.6. Source: ProtInc lamellipodium assembly Traceable author statement PubMed 9312003. Source: UniProtKB ruffle organization Traceable author statement PubMed 9312003. Source: UniProtKB small GTPase mediated signal transduction Traceable author statement Ref.6. Source: ProtInc
Cellular_component	cell cortex Inferred from direct assay PubMed 9312003. Source: UniProtKB plasma membrane Traceable author statement PubMed 9312003. Source: UniProtKB ruffle Inferred from mutant phenotype Ref.6. Source: UniProtKB
Molecular_function	GTP binding Traceable author statement Ref.6. Source: ProtInc GTP-dependent protein binding Traceable author statement PubMed 9312003. Source: UniProtKB Rac GTPase binding Traceable author statement PubMed 9312003. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARF6	P62330	4	EBI-638194,EBI-638181
RAC1	P63000	9	EBI-638194,EBI-413628

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P53365-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P53365-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MTDGILGKAA...VKKWGINTYK → MKPALCLVAMGALVMDSSPQ

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 341

341

Arfaptin-2

PRO_0000064667

Regions

Domain

121 – 321

201

AH

Natural variations

Alternative sequence

1 – 105

105

MTDGI…INTYK → MKPALCLVAMGALVMDSSPQ in isoform 2.

VSP_042524

Secondary structure

1

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341

Helix Strand Turn

Details...

Helix

124 – 173

50

Helix

176 – 178

3

Helix

179 – 215

37

Helix

217 – 245

29

Turn

247 – 249

3

Turn

252 – 254

3

Helix

260 – 316

57

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AB8D224430D8E213	FASTA	341	37,856
	10 20 30 40 50 60 MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI VSGGYGGSGD 70 80 90 100 110 120 GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG INTYKCTKQL LSERFGRGSR 130 140 150 160 170 180 TVDLELELQI ELLRETKRKY ESVLQLGRAL TAHLYSLLQT QHALGDAFAD LSQKSPELQE 190 200 210 220 230 240 EFGYNAETQK LLCKNGETLL GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT 250 260 270 280 290 300 DLEELSLGPR DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL 310 320 330 340 FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q
	Isoform 2 [UniParc]. Checksum: E94956B55C3FFF70	FASTA	256	29,044
	10 20 30 40 50 60 MKPALCLVAM GALVMDSSPQ CTKQLLSERF GRGSRTVDLE LELQIELLRE TKRKYESVLQ 70 80 90 100 110 120 LGRALTAHLY SLLQTQHALG DAFADLSQKS PELQEEFGYN AETQKLLCKN GETLLGAVNF 130 140 150 160 170 180 FVSSINTLVT KTMEDTLMTV KQYEAARLEY DAYRTDLEEL SLGPRDAGTR GRLESAQATF 190 200 210 220 230 240 QAHRDKYEKL RGDVAIKLKF LEENKIKVMH KQLLLFHNAV SAYFAGNQKQ LEQTLQQFNI 250 KLRPPGAEKP SWLEEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes." Kanoh H., Williger B.-T., Exton J.H. J. Biol. Chem. 272:5421-5429(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Uterus.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[6]	"Identification of a novel Rac1-interacting protein involved in membrane ruffling." van Aelst L., Joneson T., Bar-Sagi D. EMBO J. 15:3778-3786(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-341 (ISOFORM 1).
[7]	"ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins." Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A. J. Biol. Chem. 276:22826-22837(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARL1.
[8]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]	"The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways." Tarricone C., Xiao B., Justin N., Walker P.A., Rittinger K., Gamblin S.J., Smerdon S.J. Nature 411:215-219(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 118-341 IN COMPLEX WITH RAC1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U52522 mRNA. Translation: AAA97924.1.
AK304512 mRNA. Translation: BAG65318.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68707.1.
CH471064 Genomic DNA. Translation: EAW68708.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.

IPI

IPI00021257.
IPI00908576.

PIR

G02516.

RefSeq

NP_001229784.1. NM_001242855.1.
NP_001229785.1. NM_001242856.1.
NP_036534.1. NM_012402.3.

UniGene

Hs.732203.
Hs.75139.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I49	X-ray	2.80	A/B	118-341	[»]
1I4D	X-ray	2.50	A/B	118-341	[»]
1I4L	X-ray	2.70	A/B	118-341	[»]
1I4T	X-ray	2.60	A/B	118-341	[»]
4DCN	X-ray	3.01	C/D	118-315	[»]

ProteinModelPortal

P53365.

ModBase

Search...

Protein-protein interaction databases

IntAct

P53365. 11 interactions.

MINT

MINT-1438715.

STRING

9606.ENSP00000254584.

PTM databases

PhosphoSite

P53365.

Polymorphism databases

DMDM

1703205.

Proteomic databases

PaxDb

P53365.

PRIDE

P53365.

Protocols and materials databases

DNASU

23647.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000254584; ENSP00000254584; ENSG00000132254.
ENST00000396777; ENSP00000379998; ENSG00000132254.
ENST00000423813; ENSP00000398375; ENSG00000132254.
ENST00000445086; ENSP00000391427; ENSG00000132254.

GeneID

23647.

KEGG

hsa:23647.

UCSC

uc001mdk.3. human.

Organism-specific databases

CTD

23647.

GeneCards

GC11M006496.

HGNC

HGNC:17160. ARFIP2.

HPA

HPA038156.

MIM

601638. gene.

neXtProt

NX_P53365.

PharmGKB

PA134879398.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG302946.

HOGENOM

HOG000236308.

HOVERGEN

HBG050564.

InParanoid

P53365.

OMA

HALGDTF.

OrthoDB

EOG4M398W.

PhylomeDB

P53365.

Enzyme and pathway databases

Pathway_Interaction_DB

arf_3pathway. Arf1 pathway.

Gene expression databases

ArrayExpress

P53365.

Bgee

P53365.

CleanEx

HS_ARFIP2.

Genevestigator

P53365.

GermOnline

ENSG00000132254. Homo sapiens.

Family and domain databases

Gene3D

1.20.1270.60. 1 hit.

InterPro

IPR027267. AH/BAR-dom.
IPR010504. AH_dom.
[Graphical view]

Pfam

PF06456. Arfaptin. 1 hit.
[Graphical view]

SMART

SM01015. Arfaptin. 1 hit.
[Graphical view]

PROSITE

PS50870. AH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

ARFIP2. human.

EvolutionaryTrace

P53365.

GenomeRNAi

23647.

NextBio

46467.

SOURCE

Search...

Entry information

Entry name

ARFP2_HUMAN

Accession

Primary (citable) accession number: P53365
Secondary accession number(s): B4E306, D3DQT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.