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P53365

- ARFP2_HUMAN

UniProt

P53365 - ARFP2_HUMAN

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Protein

Arfaptin-2

Gene

ARFIP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

GO - Molecular functioni

  1. GTP binding Source: ProtInc
  2. GTP-dependent protein binding Source: UniProtKB
  3. Rac GTPase binding Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. cellular component movement Source: ProtInc
  3. lamellipodium assembly Source: UniProtKB
  4. ruffle organization Source: UniProtKB
  5. small GTPase mediated signal transduction Source: ProtInc
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Arfaptin-2
Alternative name(s):
ADP-ribosylation factor-interacting protein 2
Partner of RAC1
Short name:
Protein POR1
Gene namesi
Name:ARFIP2
Synonyms:POR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:17160. ARFIP2.

Subcellular locationi

GO - Cellular componenti

  1. cell cortex Source: UniProtKB
  2. cytoplasm Source: UniProtKB
  3. plasma membrane Source: UniProtKB
  4. ruffle Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134879398.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Arfaptin-2PRO_0000064667Add
BLAST

Proteomic databases

MaxQBiP53365.
PaxDbiP53365.
PRIDEiP53365.

PTM databases

PhosphoSiteiP53365.

Expressioni

Gene expression databases

BgeeiP53365.
CleanExiHS_ARFIP2.
ExpressionAtlasiP53365. baseline and differential.
GenevestigatoriP53365.

Organism-specific databases

HPAiHPA038156.

Interactioni

Subunit structurei

Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ARF6P623304EBI-638194,EBI-638181
RAC1P630009EBI-638194,EBI-413628

Protein-protein interaction databases

BioGridi117174. 21 interactions.
IntActiP53365. 13 interactions.
MINTiMINT-1438715.
STRINGi9606.ENSP00000254584.

Structurei

Secondary structure

1
341
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi124 – 17350Combined sources
Helixi176 – 1783Combined sources
Helixi179 – 21537Combined sources
Helixi217 – 24529Combined sources
Turni247 – 2493Combined sources
Turni252 – 2543Combined sources
Helixi260 – 31657Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I49X-ray2.80A/B118-341[»]
1I4DX-ray2.50A/B118-341[»]
1I4LX-ray2.70A/B118-341[»]
1I4TX-ray2.60A/B118-341[»]
4DCNX-ray3.01C/D118-315[»]
ProteinModelPortaliP53365.
SMRiP53365. Positions 119-319.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini121 – 321201AHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 AH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG302946.
GeneTreeiENSGT00500000044828.
HOGENOMiHOG000236308.
HOVERGENiHBG050564.
InParanoidiP53365.
OMAiHALGDTF.
OrthoDBiEOG7VQJD9.
PhylomeDBiP53365.
TreeFamiTF314945.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR010504. AH_dom.
[Graphical view]
PfamiPF06456. Arfaptin. 1 hit.
[Graphical view]
SMARTiSM01015. Arfaptin. 1 hit.
[Graphical view]
PROSITEiPS50870. AH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P53365-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI
60 70 80 90 100
VSGGYGGSGD GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG
110 120 130 140 150
INTYKCTKQL LSERFGRGSR TVDLELELQI ELLRETKRKY ESVLQLGRAL
160 170 180 190 200
TAHLYSLLQT QHALGDAFAD LSQKSPELQE EFGYNAETQK LLCKNGETLL
210 220 230 240 250
GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT DLEELSLGPR
260 270 280 290 300
DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL
310 320 330 340
FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q
Length:341
Mass (Da):37,856
Last modified:October 1, 1996 - v1
Checksum:iAB8D224430D8E213
GO
Isoform 2 (identifier: P53365-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MTDGILGKAA...VKKWGINTYK → MKPALCLVAMGALVMDSSPQ

Note: No experimental confirmation available.

Show »
Length:256
Mass (Da):29,044
Checksum:iE94956B55C3FFF70
GO
Isoform 3 (identifier: P53365-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:303
Mass (Da):33,782
Checksum:i6C52F76110467F97
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2251V → A in BAG63298. (PubMed:14702039)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 105105MTDGI…INTYK → MKPALCLVAMGALVMDSSPQ in isoform 2. 1 PublicationVSP_042524Add
BLAST
Alternative sequencei1 – 3838Missing in isoform 3. 1 PublicationVSP_046913Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52522 mRNA. Translation: AAA97924.1.
AK301856 mRNA. Translation: BAG63298.1.
AK304512 mRNA. Translation: BAG65318.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68707.1.
CH471064 Genomic DNA. Translation: EAW68708.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.
CCDSiCCDS55739.1. [P53365-2]
CCDS55740.1. [P53365-3]
CCDS7765.1. [P53365-1]
PIRiG02516.
RefSeqiNP_001229784.1. NM_001242855.1. [P53365-3]
NP_001229785.1. NM_001242856.1. [P53365-2]
NP_036534.1. NM_012402.3. [P53365-1]
XP_005252897.1. XM_005252840.2. [P53365-1]
UniGeneiHs.732203.
Hs.75139.

Genome annotation databases

EnsembliENST00000254584; ENSP00000254584; ENSG00000132254. [P53365-1]
ENST00000396777; ENSP00000379998; ENSG00000132254. [P53365-1]
ENST00000423813; ENSP00000398375; ENSG00000132254. [P53365-3]
ENST00000445086; ENSP00000391427; ENSG00000132254. [P53365-2]
GeneIDi23647.
KEGGihsa:23647.
UCSCiuc001mdk.3. human. [P53365-1]
uc010ram.2. human. [P53365-2]

Polymorphism databases

DMDMi1703205.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52522 mRNA. Translation: AAA97924.1 .
AK301856 mRNA. Translation: BAG63298.1 .
AK304512 mRNA. Translation: BAG65318.1 .
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68707.1 .
CH471064 Genomic DNA. Translation: EAW68708.1 .
BC000392 mRNA. Translation: AAH00392.1 .
X97567 mRNA. Translation: CAA66179.1 .
CCDSi CCDS55739.1. [P53365-2 ]
CCDS55740.1. [P53365-3 ]
CCDS7765.1. [P53365-1 ]
PIRi G02516.
RefSeqi NP_001229784.1. NM_001242855.1. [P53365-3 ]
NP_001229785.1. NM_001242856.1. [P53365-2 ]
NP_036534.1. NM_012402.3. [P53365-1 ]
XP_005252897.1. XM_005252840.2. [P53365-1 ]
UniGenei Hs.732203.
Hs.75139.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1I49 X-ray 2.80 A/B 118-341 [» ]
1I4D X-ray 2.50 A/B 118-341 [» ]
1I4L X-ray 2.70 A/B 118-341 [» ]
1I4T X-ray 2.60 A/B 118-341 [» ]
4DCN X-ray 3.01 C/D 118-315 [» ]
ProteinModelPortali P53365.
SMRi P53365. Positions 119-319.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 117174. 21 interactions.
IntActi P53365. 13 interactions.
MINTi MINT-1438715.
STRINGi 9606.ENSP00000254584.

PTM databases

PhosphoSitei P53365.

Polymorphism databases

DMDMi 1703205.

Proteomic databases

MaxQBi P53365.
PaxDbi P53365.
PRIDEi P53365.

Protocols and materials databases

DNASUi 23647.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000254584 ; ENSP00000254584 ; ENSG00000132254 . [P53365-1 ]
ENST00000396777 ; ENSP00000379998 ; ENSG00000132254 . [P53365-1 ]
ENST00000423813 ; ENSP00000398375 ; ENSG00000132254 . [P53365-3 ]
ENST00000445086 ; ENSP00000391427 ; ENSG00000132254 . [P53365-2 ]
GeneIDi 23647.
KEGGi hsa:23647.
UCSCi uc001mdk.3. human. [P53365-1 ]
uc010ram.2. human. [P53365-2 ]

Organism-specific databases

CTDi 23647.
GeneCardsi GC11M006496.
HGNCi HGNC:17160. ARFIP2.
HPAi HPA038156.
MIMi 601638. gene.
neXtProti NX_P53365.
PharmGKBi PA134879398.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG302946.
GeneTreei ENSGT00500000044828.
HOGENOMi HOG000236308.
HOVERGENi HBG050564.
InParanoidi P53365.
OMAi HALGDTF.
OrthoDBi EOG7VQJD9.
PhylomeDBi P53365.
TreeFami TF314945.

Miscellaneous databases

ChiTaRSi ARFIP2. human.
EvolutionaryTracei P53365.
GeneWikii ARFIP2.
GenomeRNAii 23647.
NextBioi 46467.
PROi P53365.
SOURCEi Search...

Gene expression databases

Bgeei P53365.
CleanExi HS_ARFIP2.
ExpressionAtlasi P53365. baseline and differential.
Genevestigatori P53365.

Family and domain databases

Gene3Di 1.20.1270.60. 1 hit.
InterProi IPR027267. AH/BAR-dom.
IPR010504. AH_dom.
[Graphical view ]
Pfami PF06456. Arfaptin. 1 hit.
[Graphical view ]
SMARTi SM01015. Arfaptin. 1 hit.
[Graphical view ]
PROSITEi PS50870. AH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes."
    Kanoh H., Williger B.-T., Exton J.H.
    J. Biol. Chem. 272:5421-5429(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
    Tissue: Testis and Uterus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  6. "Identification of a novel Rac1-interacting protein involved in membrane ruffling."
    van Aelst L., Joneson T., Bar-Sagi D.
    EMBO J. 15:3778-3786(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-341 (ISOFORM 1).
  7. "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins."
    Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A.
    J. Biol. Chem. 276:22826-22837(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARL1.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways."
    Tarricone C., Xiao B., Justin N., Walker P.A., Rittinger K., Gamblin S.J., Smerdon S.J.
    Nature 411:215-219(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 118-341 IN COMPLEX WITH RAC1.

Entry informationi

Entry nameiARFP2_HUMAN
AccessioniPrimary (citable) accession number: P53365
Secondary accession number(s): B4DX86, B4E306, D3DQT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3