Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P53365

- ARFP2_HUMAN

UniProt

P53365 - ARFP2_HUMAN

Protein

Arfaptin-2

Gene

ARFIP2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

    GO - Molecular functioni

    1. GTP binding Source: ProtInc
    2. GTP-dependent protein binding Source: UniProtKB
    3. protein binding Source: IntAct
    4. Rac GTPase binding Source: UniProtKB

    GO - Biological processi

    1. actin cytoskeleton organization Source: UniProtKB
    2. cellular component movement Source: ProtInc
    3. lamellipodium assembly Source: UniProtKB
    4. ruffle organization Source: UniProtKB
    5. small GTPase mediated signal transduction Source: ProtInc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arfaptin-2
    Alternative name(s):
    ADP-ribosylation factor-interacting protein 2
    Partner of RAC1
    Short name:
    Protein POR1
    Gene namesi
    Name:ARFIP2
    Synonyms:POR1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:17160. ARFIP2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB
    2. cytoplasm Source: UniProtKB
    3. plasma membrane Source: UniProtKB
    4. ruffle Source: UniProtKB

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA134879398.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 341341Arfaptin-2PRO_0000064667Add
    BLAST

    Proteomic databases

    MaxQBiP53365.
    PaxDbiP53365.
    PRIDEiP53365.

    PTM databases

    PhosphoSiteiP53365.

    Expressioni

    Gene expression databases

    ArrayExpressiP53365.
    BgeeiP53365.
    CleanExiHS_ARFIP2.
    GenevestigatoriP53365.

    Organism-specific databases

    HPAiHPA038156.

    Interactioni

    Subunit structurei

    Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARF6P623304EBI-638194,EBI-638181
    RAC1P630009EBI-638194,EBI-413628

    Protein-protein interaction databases

    BioGridi117174. 19 interactions.
    IntActiP53365. 13 interactions.
    MINTiMINT-1438715.
    STRINGi9606.ENSP00000254584.

    Structurei

    Secondary structure

    1
    341
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi124 – 17350
    Helixi176 – 1783
    Helixi179 – 21537
    Helixi217 – 24529
    Turni247 – 2493
    Turni252 – 2543
    Helixi260 – 31657

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1I49X-ray2.80A/B118-341[»]
    1I4DX-ray2.50A/B118-341[»]
    1I4LX-ray2.70A/B118-341[»]
    1I4TX-ray2.60A/B118-341[»]
    4DCNX-ray3.01C/D118-315[»]
    ProteinModelPortaliP53365.
    SMRiP53365. Positions 119-319.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP53365.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini121 – 321201AHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 AH domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG302946.
    HOGENOMiHOG000236308.
    HOVERGENiHBG050564.
    InParanoidiP53365.
    OMAiHALGDTF.
    OrthoDBiEOG7VQJD9.
    PhylomeDBiP53365.
    TreeFamiTF314945.

    Family and domain databases

    Gene3Di1.20.1270.60. 1 hit.
    InterProiIPR027267. AH/BAR-dom.
    IPR010504. AH_dom.
    [Graphical view]
    PfamiPF06456. Arfaptin. 1 hit.
    [Graphical view]
    SMARTiSM01015. Arfaptin. 1 hit.
    [Graphical view]
    PROSITEiPS50870. AH. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P53365-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI    50
    VSGGYGGSGD GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG 100
    INTYKCTKQL LSERFGRGSR TVDLELELQI ELLRETKRKY ESVLQLGRAL 150
    TAHLYSLLQT QHALGDAFAD LSQKSPELQE EFGYNAETQK LLCKNGETLL 200
    GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT DLEELSLGPR 250
    DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL 300
    FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q 341
    Length:341
    Mass (Da):37,856
    Last modified:October 1, 1996 - v1
    Checksum:iAB8D224430D8E213
    GO
    Isoform 2 (identifier: P53365-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-105: MTDGILGKAA...VKKWGINTYK → MKPALCLVAMGALVMDSSPQ

    Note: No experimental confirmation available.

    Show »
    Length:256
    Mass (Da):29,044
    Checksum:iE94956B55C3FFF70
    GO
    Isoform 3 (identifier: P53365-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-38: Missing.

    Show »
    Length:303
    Mass (Da):33,782
    Checksum:i6C52F76110467F97
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti225 – 2251V → A in BAG63298. (PubMed:14702039)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 105105MTDGI…INTYK → MKPALCLVAMGALVMDSSPQ in isoform 2. 1 PublicationVSP_042524Add
    BLAST
    Alternative sequencei1 – 3838Missing in isoform 3. 1 PublicationVSP_046913Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52522 mRNA. Translation: AAA97924.1.
    AK301856 mRNA. Translation: BAG63298.1.
    AK304512 mRNA. Translation: BAG65318.1.
    AC084337 Genomic DNA. No translation available.
    CH471064 Genomic DNA. Translation: EAW68707.1.
    CH471064 Genomic DNA. Translation: EAW68708.1.
    BC000392 mRNA. Translation: AAH00392.1.
    X97567 mRNA. Translation: CAA66179.1.
    CCDSiCCDS55739.1. [P53365-2]
    CCDS55740.1. [P53365-3]
    CCDS7765.1. [P53365-1]
    PIRiG02516.
    RefSeqiNP_001229784.1. NM_001242855.1. [P53365-3]
    NP_001229785.1. NM_001242856.1. [P53365-2]
    NP_036534.1. NM_012402.3. [P53365-1]
    XP_005252897.1. XM_005252840.2. [P53365-1]
    UniGeneiHs.732203.
    Hs.75139.

    Genome annotation databases

    EnsembliENST00000254584; ENSP00000254584; ENSG00000132254. [P53365-1]
    ENST00000396777; ENSP00000379998; ENSG00000132254. [P53365-1]
    ENST00000423813; ENSP00000398375; ENSG00000132254. [P53365-3]
    ENST00000445086; ENSP00000391427; ENSG00000132254. [P53365-2]
    GeneIDi23647.
    KEGGihsa:23647.
    UCSCiuc001mdk.3. human. [P53365-1]
    uc010ram.2. human. [P53365-2]

    Polymorphism databases

    DMDMi1703205.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52522 mRNA. Translation: AAA97924.1 .
    AK301856 mRNA. Translation: BAG63298.1 .
    AK304512 mRNA. Translation: BAG65318.1 .
    AC084337 Genomic DNA. No translation available.
    CH471064 Genomic DNA. Translation: EAW68707.1 .
    CH471064 Genomic DNA. Translation: EAW68708.1 .
    BC000392 mRNA. Translation: AAH00392.1 .
    X97567 mRNA. Translation: CAA66179.1 .
    CCDSi CCDS55739.1. [P53365-2 ]
    CCDS55740.1. [P53365-3 ]
    CCDS7765.1. [P53365-1 ]
    PIRi G02516.
    RefSeqi NP_001229784.1. NM_001242855.1. [P53365-3 ]
    NP_001229785.1. NM_001242856.1. [P53365-2 ]
    NP_036534.1. NM_012402.3. [P53365-1 ]
    XP_005252897.1. XM_005252840.2. [P53365-1 ]
    UniGenei Hs.732203.
    Hs.75139.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1I49 X-ray 2.80 A/B 118-341 [» ]
    1I4D X-ray 2.50 A/B 118-341 [» ]
    1I4L X-ray 2.70 A/B 118-341 [» ]
    1I4T X-ray 2.60 A/B 118-341 [» ]
    4DCN X-ray 3.01 C/D 118-315 [» ]
    ProteinModelPortali P53365.
    SMRi P53365. Positions 119-319.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 117174. 19 interactions.
    IntActi P53365. 13 interactions.
    MINTi MINT-1438715.
    STRINGi 9606.ENSP00000254584.

    PTM databases

    PhosphoSitei P53365.

    Polymorphism databases

    DMDMi 1703205.

    Proteomic databases

    MaxQBi P53365.
    PaxDbi P53365.
    PRIDEi P53365.

    Protocols and materials databases

    DNASUi 23647.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000254584 ; ENSP00000254584 ; ENSG00000132254 . [P53365-1 ]
    ENST00000396777 ; ENSP00000379998 ; ENSG00000132254 . [P53365-1 ]
    ENST00000423813 ; ENSP00000398375 ; ENSG00000132254 . [P53365-3 ]
    ENST00000445086 ; ENSP00000391427 ; ENSG00000132254 . [P53365-2 ]
    GeneIDi 23647.
    KEGGi hsa:23647.
    UCSCi uc001mdk.3. human. [P53365-1 ]
    uc010ram.2. human. [P53365-2 ]

    Organism-specific databases

    CTDi 23647.
    GeneCardsi GC11M006496.
    HGNCi HGNC:17160. ARFIP2.
    HPAi HPA038156.
    MIMi 601638. gene.
    neXtProti NX_P53365.
    PharmGKBi PA134879398.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG302946.
    HOGENOMi HOG000236308.
    HOVERGENi HBG050564.
    InParanoidi P53365.
    OMAi HALGDTF.
    OrthoDBi EOG7VQJD9.
    PhylomeDBi P53365.
    TreeFami TF314945.

    Miscellaneous databases

    ChiTaRSi ARFIP2. human.
    EvolutionaryTracei P53365.
    GeneWikii ARFIP2.
    GenomeRNAii 23647.
    NextBioi 46467.
    PROi P53365.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P53365.
    Bgeei P53365.
    CleanExi HS_ARFIP2.
    Genevestigatori P53365.

    Family and domain databases

    Gene3Di 1.20.1270.60. 1 hit.
    InterProi IPR027267. AH/BAR-dom.
    IPR010504. AH_dom.
    [Graphical view ]
    Pfami PF06456. Arfaptin. 1 hit.
    [Graphical view ]
    SMARTi SM01015. Arfaptin. 1 hit.
    [Graphical view ]
    PROSITEi PS50870. AH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes."
      Kanoh H., Williger B.-T., Exton J.H.
      J. Biol. Chem. 272:5421-5429(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
      Tissue: Testis and Uterus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Lung.
    6. "Identification of a novel Rac1-interacting protein involved in membrane ruffling."
      van Aelst L., Joneson T., Bar-Sagi D.
      EMBO J. 15:3778-3786(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-341 (ISOFORM 1).
    7. "ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins."
      Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A.
      J. Biol. Chem. 276:22826-22837(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARL1.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways."
      Tarricone C., Xiao B., Justin N., Walker P.A., Rittinger K., Gamblin S.J., Smerdon S.J.
      Nature 411:215-219(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 118-341 IN COMPLEX WITH RAC1.

    Entry informationi

    Entry nameiARFP2_HUMAN
    AccessioniPrimary (citable) accession number: P53365
    Secondary accession number(s): B4DX86, B4E306, D3DQT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3