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Protein

Arfaptin-2

Gene

ARFIP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • GTP binding Source: ProtInc
  • GTP-dependent protein binding Source: UniProtKB
  • phosphatidylinositol-4-phosphate binding Source: FlyBase
  • Rac GTPase binding Source: UniProtKB

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • lamellipodium assembly Source: UniProtKB
  • movement of cell or subcellular component Source: ProtInc
  • regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
  • ruffle organization Source: UniProtKB
  • small GTPase mediated signal transduction Source: ProtInc
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132254-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
SIGNORiP53365.

Names & Taxonomyi

Protein namesi
Recommended name:
Arfaptin-2
Alternative name(s):
ADP-ribosylation factor-interacting protein 2
Partner of RAC1
Short name:
Protein POR1
Gene namesi
Name:ARFIP2
Synonyms:POR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:17160. ARFIP2.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cell cortex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: Reactome
  • plasma membrane Source: UniProtKB
  • ruffle Source: UniProtKB
  • trans-Golgi network membrane Source: FlyBase
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000132254.
PharmGKBiPA134879398.

Polymorphism and mutation databases

BioMutaiARFIP2.
DMDMi1703205.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000646671 – 341Arfaptin-2Add BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei72PhosphoserineCombined sources1
Modified residuei76PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP53365.
MaxQBiP53365.
PaxDbiP53365.
PeptideAtlasiP53365.
PRIDEiP53365.

PTM databases

iPTMnetiP53365.
PhosphoSitePlusiP53365.

Expressioni

Gene expression databases

BgeeiENSG00000132254.
CleanExiHS_ARFIP2.
ExpressionAtlasiP53365. baseline and differential.
GenevisibleiP53365. HS.

Organism-specific databases

HPAiCAB032985.
HPA038156.

Interactioni

Subunit structurei

Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-638194,EBI-638194
AGTRAPQ6RW134EBI-638194,EBI-741181
ARF6P623304EBI-638194,EBI-638181
CEP63Q96MT83EBI-638194,EBI-741977
HAUS1Q96CS23EBI-638194,EBI-2514791
HGSO149643EBI-638194,EBI-740220
ING5Q8WYH83EBI-638194,EBI-488533
ITGB3BPQ133524EBI-638194,EBI-712105
ITGB3BPQ13352-53EBI-638194,EBI-10175826
KRT15P190123EBI-638194,EBI-739566
MAL2Q969L23EBI-638194,EBI-944295
RAC1P630009EBI-638194,EBI-413628
TRIM54Q9BYV23EBI-638194,EBI-2130429
USHBP1Q8N6Y03EBI-638194,EBI-739895

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL
  • GTP-dependent protein binding Source: UniProtKB
  • Rac GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi117174. 37 interactors.
IntActiP53365. 28 interactors.
MINTiMINT-1438715.
STRINGi9606.ENSP00000254584.

Structurei

Secondary structure

1341
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi124 – 173Combined sources50
Helixi176 – 178Combined sources3
Helixi179 – 215Combined sources37
Helixi217 – 245Combined sources29
Turni247 – 249Combined sources3
Turni252 – 254Combined sources3
Helixi260 – 316Combined sources57

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I49X-ray2.80A/B118-341[»]
1I4DX-ray2.50A/B118-341[»]
1I4LX-ray2.70A/B118-341[»]
1I4TX-ray2.60A/B118-341[»]
4DCNX-ray3.01C/D118-315[»]
ProteinModelPortaliP53365.
SMRiP53365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP53365.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini121 – 321AHPROSITE-ProRule annotationAdd BLAST201

Sequence similaritiesi

Contains 1 AH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3876. Eukaryota.
ENOG410YJUM. LUCA.
GeneTreeiENSGT00500000044828.
HOGENOMiHOG000236308.
HOVERGENiHBG050564.
InParanoidiP53365.
KOiK20314.
PhylomeDBiP53365.
TreeFamiTF314945.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR010504. AH_dom.
IPR030798. Arfaptin_fam.
[Graphical view]
PANTHERiPTHR12141. PTHR12141. 1 hit.
PfamiPF06456. Arfaptin. 1 hit.
[Graphical view]
SMARTiSM01015. Arfaptin. 1 hit.
[Graphical view]
PROSITEiPS50870. AH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P53365-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI
60 70 80 90 100
VSGGYGGSGD GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG
110 120 130 140 150
INTYKCTKQL LSERFGRGSR TVDLELELQI ELLRETKRKY ESVLQLGRAL
160 170 180 190 200
TAHLYSLLQT QHALGDAFAD LSQKSPELQE EFGYNAETQK LLCKNGETLL
210 220 230 240 250
GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT DLEELSLGPR
260 270 280 290 300
DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL
310 320 330 340
FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q
Length:341
Mass (Da):37,856
Last modified:October 1, 1996 - v1
Checksum:iAB8D224430D8E213
GO
Isoform 2 (identifier: P53365-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MTDGILGKAA...VKKWGINTYK → MKPALCLVAMGALVMDSSPQ

Note: No experimental confirmation available.
Show »
Length:256
Mass (Da):29,044
Checksum:iE94956B55C3FFF70
GO
Isoform 3 (identifier: P53365-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:303
Mass (Da):33,782
Checksum:i6C52F76110467F97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225V → A in BAG63298 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0425241 – 105MTDGI…INTYK → MKPALCLVAMGALVMDSSPQ in isoform 2. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_0469131 – 38Missing in isoform 3. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52522 mRNA. Translation: AAA97924.1.
AK301856 mRNA. Translation: BAG63298.1.
AK304512 mRNA. Translation: BAG65318.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68707.1.
CH471064 Genomic DNA. Translation: EAW68708.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.
CCDSiCCDS55739.1. [P53365-2]
CCDS55740.1. [P53365-3]
CCDS7765.1. [P53365-1]
PIRiG02516.
RefSeqiNP_001229784.1. NM_001242855.1. [P53365-3]
NP_001229785.1. NM_001242856.1. [P53365-2]
NP_036534.1. NM_012402.3. [P53365-1]
XP_005252897.1. XM_005252840.4. [P53365-1]
UniGeneiHs.732203.
Hs.75139.

Genome annotation databases

EnsembliENST00000254584; ENSP00000254584; ENSG00000132254. [P53365-1]
ENST00000396777; ENSP00000379998; ENSG00000132254. [P53365-1]
ENST00000423813; ENSP00000398375; ENSG00000132254. [P53365-3]
ENST00000445086; ENSP00000391427; ENSG00000132254. [P53365-2]
GeneIDi23647.
KEGGihsa:23647.
UCSCiuc001mdk.4. human. [P53365-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52522 mRNA. Translation: AAA97924.1.
AK301856 mRNA. Translation: BAG63298.1.
AK304512 mRNA. Translation: BAG65318.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68707.1.
CH471064 Genomic DNA. Translation: EAW68708.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.
CCDSiCCDS55739.1. [P53365-2]
CCDS55740.1. [P53365-3]
CCDS7765.1. [P53365-1]
PIRiG02516.
RefSeqiNP_001229784.1. NM_001242855.1. [P53365-3]
NP_001229785.1. NM_001242856.1. [P53365-2]
NP_036534.1. NM_012402.3. [P53365-1]
XP_005252897.1. XM_005252840.4. [P53365-1]
UniGeneiHs.732203.
Hs.75139.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1I49X-ray2.80A/B118-341[»]
1I4DX-ray2.50A/B118-341[»]
1I4LX-ray2.70A/B118-341[»]
1I4TX-ray2.60A/B118-341[»]
4DCNX-ray3.01C/D118-315[»]
ProteinModelPortaliP53365.
SMRiP53365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117174. 37 interactors.
IntActiP53365. 28 interactors.
MINTiMINT-1438715.
STRINGi9606.ENSP00000254584.

PTM databases

iPTMnetiP53365.
PhosphoSitePlusiP53365.

Polymorphism and mutation databases

BioMutaiARFIP2.
DMDMi1703205.

Proteomic databases

EPDiP53365.
MaxQBiP53365.
PaxDbiP53365.
PeptideAtlasiP53365.
PRIDEiP53365.

Protocols and materials databases

DNASUi23647.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000254584; ENSP00000254584; ENSG00000132254. [P53365-1]
ENST00000396777; ENSP00000379998; ENSG00000132254. [P53365-1]
ENST00000423813; ENSP00000398375; ENSG00000132254. [P53365-3]
ENST00000445086; ENSP00000391427; ENSG00000132254. [P53365-2]
GeneIDi23647.
KEGGihsa:23647.
UCSCiuc001mdk.4. human. [P53365-1]

Organism-specific databases

CTDi23647.
GeneCardsiARFIP2.
HGNCiHGNC:17160. ARFIP2.
HPAiCAB032985.
HPA038156.
MIMi601638. gene.
neXtProtiNX_P53365.
OpenTargetsiENSG00000132254.
PharmGKBiPA134879398.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3876. Eukaryota.
ENOG410YJUM. LUCA.
GeneTreeiENSGT00500000044828.
HOGENOMiHOG000236308.
HOVERGENiHBG050564.
InParanoidiP53365.
KOiK20314.
PhylomeDBiP53365.
TreeFamiTF314945.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000132254-MONOMER.
ReactomeiR-HSA-6811440. Retrograde transport at the Trans-Golgi-Network.
SIGNORiP53365.

Miscellaneous databases

ChiTaRSiARFIP2. human.
EvolutionaryTraceiP53365.
GeneWikiiARFIP2.
GenomeRNAii23647.
PROiP53365.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000132254.
CleanExiHS_ARFIP2.
ExpressionAtlasiP53365. baseline and differential.
GenevisibleiP53365. HS.

Family and domain databases

Gene3Di1.20.1270.60. 1 hit.
InterProiIPR027267. AH/BAR-dom.
IPR010504. AH_dom.
IPR030798. Arfaptin_fam.
[Graphical view]
PANTHERiPTHR12141. PTHR12141. 1 hit.
PfamiPF06456. Arfaptin. 1 hit.
[Graphical view]
SMARTiSM01015. Arfaptin. 1 hit.
[Graphical view]
PROSITEiPS50870. AH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARFP2_HUMAN
AccessioniPrimary (citable) accession number: P53365
Secondary accession number(s): B4DX86, B4E306, D3DQT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.