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Reviewed, UniProtKB/Swiss-Prot P53365 (ARFP2_HUMAN)

Last modified November 25, 2008. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Arfaptin-2
Alternative name(s):
    ADP-ribosylation factor-interacting protein 2
    Partner of RAC1
      Short name=Protein POR1
Gene names
Name: ARFIP2
Synonyms: POR1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

Subunit structure

Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities.

Sequence similarities

Contains 1 AH domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Arfaptin-2
PRO_0000064667

Regions

Domain121 – 321201AH

Secondary structure

.......... 341
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53365-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: AB8D224430D8E213

FASTA34137,856
        10         20         30         40         50         60 
MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI VSGGYGGSGD 

        70         80         90        100        110        120 
GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG INTYKCTKQL LSERFGRGSR 

       130        140        150        160        170        180 
TVDLELELQI ELLRETKRKY ESVLQLGRAL TAHLYSLLQT QHALGDAFAD LSQKSPELQE 

       190        200        210        220        230        240 
EFGYNAETQK LLCKNGETLL GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT 

       250        260        270        280        290        300 
DLEELSLGPR DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL 

       310        320        330        340 
FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q 

« Hide

References

« Hide 'large scale' references
[1]"Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes."
Kanoh H., Williger B.-T., Exton J.H.
J. Biol. Chem. 272:5421-5429(1997) [PubMed: 9038142] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[3]"Identification of a novel Rac1-interacting protein involved in membrane ruffling."
van Aelst L., Joneson T., Bar-Sagi D.
EMBO J. 15:3778-3786(1996) [PubMed: 8670882] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-341.
[4]"The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways."
Tarricone C., Xiao B., Justin N., Walker P.A., Rittinger K., Gamblin S.J., Smerdon S.J.
Nature 411:215-219(2001) [PubMed: 11346801] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 118-341 IN COMPLEX WITH RAC1.
+Additional computationally mapped references.

Cross-references

Sequence databases

U52522 mRNA. Translation: AAA97924.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.
PIRG02516.
RefSeqNP_036534.1.
UniGeneHs.75139

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1I49X-ray2.80A/B118-341[»]
1I4DX-ray2.50A/B118-341[»]
1I4LX-ray2.70A/B118-341[»]
1I4TX-ray2.60A/B118-341[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP53365.

PTM databases

PhosphoSiteP53365.

Genome annotation databases

EnsemblENSG00000132254. Homo sapiens. [Contig view]
GeneID23647.
KEGGhsa:23647.

Organism-specific databases

H-InvDBHIX0022270.
HGNCHGNC:17160. ARFIP2.
MIM601638. gene.
PharmGKBPA134879398.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP53365.
HOVERGENP53365.

Gene expression databases

ArrayExpressP53365.
CleanExHS_ARFIP2.
GermOnlineENSG00000132254. Homo sapiens.

Family and domain databases

InterProIPR010504. Arfaptin.
[Graphical view]
Gene3DG3DSA:1.20.1270.60. Arfaptin. 1 hit.
PfamPF06456. Arfaptin. 1 hit.
[Graphical view]
PROSITEPS50870. AH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP53365.
NextBio46467.
SOURCESearch...

Entry information

Entry nameARFP2_HUMAN
AccessionPrimary (citable) accession number: P53365
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 25, 2008
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents