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P53365 (ARFP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Arfaptin-2
Alternative name(s):
ADP-ribosylation factor-interacting protein 2
Partner of RAC1
Short name=Protein POR1
Gene names
Name:ARFIP2
Synonyms:POR1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Putative target protein of ADP-ribosylation factor. Involved in membrane ruffling.

Subunit structure

Interacts with RAC1 by binding directly to it. Specifically binds to GTP-bound ARF1 and ARF6, but binds to RAC1.GTP and RAC1.GDP with similar affinities. Directly interacts with ARL1 GTP-bound form; this interaction leads to a > 4-fold increase in the amount of ARL1-bound GTP at steady state level. Ref.7

Sequence similarities

Contains 1 AH domain.

Binary interactions

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P53365-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P53365-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: MTDGILGKAA...VKKWGINTYK → MKPALCLVAMGALVMDSSPQ
Note: No experimental confirmation available.
Isoform 3 (identifier: P53365-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Arfaptin-2
PRO_0000064667

Regions

Domain121 – 321201AH

Natural variations

Alternative sequence1 – 105105MTDGI…INTYK → MKPALCLVAMGALVMDSSPQ in isoform 2.
VSP_042524
Alternative sequence1 – 3838Missing in isoform 3.
VSP_046913

Experimental info

Sequence conflict2251V → A in BAG63298. Ref.2

Secondary structure

.............. 341
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: AB8D224430D8E213

FASTA34137,856
        10         20         30         40         50         60 
MTDGILGKAA TMEIPIHGNG EARQLPEDDG LEQDLQQVMV SGPNLNETSI VSGGYGGSGD 

        70         80         90        100        110        120 
GLIPTGSGRH PSHSTTPSGP GDEVARGIAG EKFDIVKKWG INTYKCTKQL LSERFGRGSR 

       130        140        150        160        170        180 
TVDLELELQI ELLRETKRKY ESVLQLGRAL TAHLYSLLQT QHALGDAFAD LSQKSPELQE 

       190        200        210        220        230        240 
EFGYNAETQK LLCKNGETLL GAVNFFVSSI NTLVTKTMED TLMTVKQYEA ARLEYDAYRT 

       250        260        270        280        290        300 
DLEELSLGPR DAGTRGRLES AQATFQAHRD KYEKLRGDVA IKLKFLEENK IKVMHKQLLL 

       310        320        330        340 
FHNAVSAYFA GNQKQLEQTL QQFNIKLRPP GAEKPSWLEE Q 

« Hide

Isoform 2 [UniParc].

Checksum: E94956B55C3FFF70
Show »

FASTA25629,044
Isoform 3 [UniParc].

Checksum: 6C52F76110467F97
Show »

FASTA30333,782

References

« Hide 'large scale' references
[1]"Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes."
Kanoh H., Williger B.-T., Exton J.H.
J. Biol. Chem. 272:5421-5429(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Tissue: Testis and Uterus.
[3]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Lung.
[6]"Identification of a novel Rac1-interacting protein involved in membrane ruffling."
van Aelst L., Joneson T., Bar-Sagi D.
EMBO J. 15:3778-3786(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-341 (ISOFORM 1).
[7]"ADP-ribosylation factors (ARFs) and ARF-like 1 (ARL1) have both specific and shared effectors: characterizing ARL1-binding proteins."
Van Valkenburgh H., Shern J.F., Sharer J.D., Zhu X., Kahn R.A.
J. Biol. Chem. 276:22826-22837(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARL1.
[8]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[9]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The structural basis of Arfaptin-mediated cross-talk between Rac and Arf signalling pathways."
Tarricone C., Xiao B., Justin N., Walker P.A., Rittinger K., Gamblin S.J., Smerdon S.J.
Nature 411:215-219(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 118-341 IN COMPLEX WITH RAC1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52522 mRNA. Translation: AAA97924.1.
AK301856 mRNA. Translation: BAG63298.1.
AK304512 mRNA. Translation: BAG65318.1.
AC084337 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68707.1.
CH471064 Genomic DNA. Translation: EAW68708.1.
BC000392 mRNA. Translation: AAH00392.1.
X97567 mRNA. Translation: CAA66179.1.
CCDSCCDS55739.1. [P53365-2]
CCDS55740.1. [P53365-3]
CCDS7765.1. [P53365-1]
PIRG02516.
RefSeqNP_001229784.1. NM_001242855.1. [P53365-3]
NP_001229785.1. NM_001242856.1. [P53365-2]
NP_036534.1. NM_012402.3. [P53365-1]
XP_005252897.1. XM_005252840.2. [P53365-1]
UniGeneHs.732203.
Hs.75139.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1I49X-ray2.80A/B118-341[»]
1I4DX-ray2.50A/B118-341[»]
1I4LX-ray2.70A/B118-341[»]
1I4TX-ray2.60A/B118-341[»]
4DCNX-ray3.01C/D118-315[»]
ProteinModelPortalP53365.
SMRP53365. Positions 119-319.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid117174. 19 interactions.
IntActP53365. 13 interactions.
MINTMINT-1438715.
STRING9606.ENSP00000254584.

PTM databases

PhosphoSiteP53365.

Polymorphism databases

DMDM1703205.

Proteomic databases

MaxQBP53365.
PaxDbP53365.
PRIDEP53365.

Protocols and materials databases

DNASU23647.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000254584; ENSP00000254584; ENSG00000132254. [P53365-1]
ENST00000396777; ENSP00000379998; ENSG00000132254. [P53365-1]
ENST00000423813; ENSP00000398375; ENSG00000132254. [P53365-3]
ENST00000445086; ENSP00000391427; ENSG00000132254. [P53365-2]
GeneID23647.
KEGGhsa:23647.
UCSCuc001mdk.3. human. [P53365-1]
uc010ram.2. human. [P53365-2]

Organism-specific databases

CTD23647.
GeneCardsGC11M006496.
HGNCHGNC:17160. ARFIP2.
HPAHPA038156.
MIM601638. gene.
neXtProtNX_P53365.
PharmGKBPA134879398.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG302946.
HOGENOMHOG000236308.
HOVERGENHBG050564.
InParanoidP53365.
OMAHALGDTF.
OrthoDBEOG7VQJD9.
PhylomeDBP53365.
TreeFamTF314945.

Gene expression databases

ArrayExpressP53365.
BgeeP53365.
CleanExHS_ARFIP2.
GenevestigatorP53365.

Family and domain databases

Gene3D1.20.1270.60. 1 hit.
InterProIPR027267. AH/BAR-dom.
IPR010504. AH_dom.
[Graphical view]
PfamPF06456. Arfaptin. 1 hit.
[Graphical view]
SMARTSM01015. Arfaptin. 1 hit.
[Graphical view]
PROSITEPS50870. AH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSARFIP2. human.
EvolutionaryTraceP53365.
GeneWikiARFIP2.
GenomeRNAi23647.
NextBio46467.
PROP53365.
SOURCESearch...

Entry information

Entry nameARFP2_HUMAN
AccessionPrimary (citable) accession number: P53365
Secondary accession number(s): B4DX86, B4E306, D3DQT5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM