Reviewed, UniProtKB/Swiss-Prot P53357 (PA12_DOLMA)

Names and origin

Protein names	Recommended name:     Phospholipase A1 2     EC=3.1.1.32     EC=3.1.1.4 Alternative name(s):     Allergen Dol m I     Allergen=Dol m 1.02
Organism	Dolichovespula maculata (White-face hornet) (Bald-faced hornet)
Taxonomic identifier	7441 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Dolichovespula

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.
Catalytic activity	Phosphatidylcholine + H(2)O = 2-acylglycerophosphocholine + a carboxylate. Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains six disulfide bonds By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Disease	Allergen
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	phospholipase A1 activity Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Phospholipase A1 2		PRO_0000090375
Sites
	Active site	140	1	Charge relay system By similarity
	Active site	168	1	Charge relay system By similarity
	Active site	232	1	Charge relay system By similarity
Natural variations
	Natural variant	55	1	G → E
	Natural variant	295	1	F → Y

Sequences

	Sequence		Length	Mass (Da)
	P53357-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 85816A837C0F3AF8	FASTA	303	33,782
	10 20 30 40 50 60 GILPECKLVP EEISFVLSTR ENRDGVYLTL QKLKNGKMFK NSDLSSKKVP FLIHGFISSA 70 80 90 100 110 120 TNKNYADMTR ALLDKDDIMV ISIDWRDGAC SNEFALLKFI GYPKAVENTR AVGKYIADFS 130 140 150 160 170 180 KILIQKYKVL LENIRLIGHS LGAQIAGFAG KEFQRFKLGK YPEIIGLDPA GPSFKKKDCP 190 200 210 220 230 240 ERICETDAHY VQILHTSSNL GTERTLGTVD FYINDGSNQP GCTYIIGETC SHTRAVKYLT 250 260 270 280 290 300 ECIRRECCLI GVPQSKNPQP VSKCTRNECV CVGLNAKEYP KKGSFYVPVE AKAPFCNNNG KII

References

[1]	"Allergens in hymenoptera venom. XXVI: the complete amino acid sequences of two vespid venom phospholipases." Hoffman D.R. Int. Arch. Allergy Immunol. 104:184-190(1994) [PubMed: 8199462] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	A44563.
3D structure databases
HSSP	HSSP built from PDB template 1BU8 based on UniProtKB P54318.
ModBase	Search...
Family and domain databases
InterPro	IPR002334. Dol/Ves_allerg. IPR000734. Lipase. IPR008262. Lipase_AS. IPR013818. Lipase_N. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit.
Pfam	PF00151. Lipase. 1 hit. [Graphical view]
PRINTS	PR00825. DOLALLERGEN. PR00821. TAGLIPASE.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA12_DOLMA

Accession

Primary (citable) accession number: P53357

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)