P53356 (HTK16_HYDVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase HTK16
EC=2.7.10.2

Gene names

Name:

HTK16

Organism

Hydra vulgaris (Hydra) (Hydra attenuata)

Taxonomic identifier

6087 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Hydrozoa › Hydroida › Anthomedusae › Hydridae › Hydra

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be involved in signal transduction.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Tissue specificity	Epithelial cells.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 5 ANK repeats. Contains 1 protein kinase domain. Contains 2 SH2 domains.

Ontologies

Keywords
Domain	ANK repeat Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 757	757	Tyrosine-protein kinase HTK16		PRO_0000088105
Regions
	Domain	10 – 102	93	SH2 1
	Repeat	115 – 147	33	ANK 1
	Repeat	151 – 180	30	ANK 2
	Repeat	184 – 214	31	ANK 3
	Repeat	219 – 248	30	ANK 4
	Repeat	252 – 281	30	ANK 5
	Domain	287 – 379	93	SH2 2
	Domain	484 – 740	257	Protein kinase
	Nucleotide binding	490 – 498	9	ATP By similarity
Sites
	Active site	608	1	Proton acceptor By similarity
	Binding site	516	1	ATP By similarity
Amino acid modifications
	Modified residue	746	1	Phosphotyrosine Potential

Sequences

	Sequence		Length	Mass (Da)
	P53356 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 144D09E9109D794F	FASTA	757	85,598
	10 20 30 40 50 60 MSKNSDALLW YHGKITREVA VQVLLRKGGR DGFFLIRDCG NAPEDYVLSM MFRSQILHFQ 70 80 90 100 110 120 INCLGDNKFS IDNGPIFQGL DMLISYYKVI SDGLPCKLVD FCVGKIAPLY ALKYGLDTRL 130 140 150 160 170 180 HLACEEKNPN TVKELLQDSV IKENVNARSI SGLTALHISC SNGDNDIVAM LLNAGADASA 190 200 210 220 230 240 IDANGRTPVQ VVCFYNHAST LHLLISKGSA DFLKRSPNNG WVPLHEAAMR GSLECVKVLL 250 260 270 280 290 300 SFNASMYPRS LDGDTPRDLA LQYENYNVVE FFDNYPVNQP KTSITQWLHQ NLDRNGALII 310 320 330 340 350 360 LQNASMADGS FLIRSSIKCH GYYVLTLVYE KKTYHFQIKS RADRWFYIDD GPLFETLPHL 370 380 390 400 410 420 VDHYMQYADG LPTLLQFPVP SAENRKRPLP PTPTKNQLKL PVPPSRPIKN NNGLPQPLPY 430 440 450 460 470 480 PEFTNESDSD IFTRLECEKE KPLPKLPRPV VNHTEVPNSV NVGQKGDQTM KNNAQQNIIL 490 500 510 520 530 540 KESISFGKEL GVGEFGSVIK GIWLSPGGKE INVAMKTLHK DKMVQGEKEF LREALVMSQL 550 560 570 580 590 600 NHPCIVSLLG VCLGPPMILV QELVEMGALL DYLMDYQPEI QEVDLKLWAS QIAFGMMYLE 610 620 630 640 650 660 LKRFVHRDLA ARNILLANKK QVKISDFGLS RAVGTGSDYY QAKQGGRWPV RWYAPESINY 670 680 690 700 710 720 GTFSTKSDVW SYGITLWEMF TFGDLPYGEM TGNEVVSFLE HCGRLEKPDE CPIHTYSIML 730 740 750 SCWHIDPNKR PTFNELHSTF STDPEYEDVR IYRDRIK

References

[1]

"Identification of a gene encoding a novel protein-tyrosine kinase containing SH2 domains and ankyrin-like repeats." Chan T.A., Chu C.A., Rauen K.A., Kroiher M., Tatarewicz S.M., Steele R.E. Oncogene 9:1253-1259(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Irvine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00936 mRNA. Translation: AAC27350.1.
3D structure databases
ProteinModelPortal	P53356.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.10.2. 2720.
Family and domain databases
Gene3D	1.25.40.20. 1 hit. 3.30.505.10. 2 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF00023. Ank. 3 hits. PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 2 hits. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SMART	SM00248. ANK. 5 hits. SM00252. SH2. 2 hits. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HTK16_HYDVU

Accession

Primary (citable) accession number: P53356

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)