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Protein

Alpha-amylase I

Gene

AMY1

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi356 – 3561CalciumBy similarity
Metal bindingi408 – 4081Calcium; via carbonyl oxygenBy similarity
Metal bindingi417 – 4171CalciumBy similarity
Binding sitei445 – 4451ChlorideBy similarity
Active sitei447 – 4471NucleophileBy similarity
Metal bindingi451 – 4511Calcium; via carbonyl oxygenBy similarity
Active sitei484 – 4841Proton donorBy similarity
Binding sitei550 – 5501ChlorideBy similarity
Sitei552 – 5521Transition state stabilizerBy similarity
Binding sitei589 – 5891ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase I (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY1
Synonyms:AMY I
ORF Names:AAEL006719
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1616Sequence analysisAdd
BLAST
Chaini17 – 737721Alpha-amylase IPRO_0000001359Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi40 – 401N-linked (GlcNAc...)Sequence analysis
Glycosylationi166 – 1661N-linked (GlcNAc...)Sequence analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence analysis
Glycosylationi233 – 2331N-linked (GlcNAc...)Sequence analysis
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence analysis
Disulfide bondi286 ↔ 342By similarity
Glycosylationi388 – 3881N-linked (GlcNAc...)Sequence analysis
Disulfide bondi396 ↔ 410By similarity
Glycosylationi403 – 4031N-linked (GlcNAc...)Sequence analysis
Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence analysis
Glycosylationi595 – 5951N-linked (GlcNAc...)Sequence analysis
Glycosylationi619 – 6191N-linked (GlcNAc...)Sequence analysis
Disulfide bondi622 ↔ 628By similarity
Glycosylationi673 – 6731N-linked (GlcNAc...)Sequence analysis
Disulfide bondi693 ↔ 705By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed specifically in the proximal-lateral lobes of the adult female salivary gland.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7159.AAEL006719-PA.

Structurei

3D structure databases

ProteinModelPortaliP53354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni17 – 260244Not found in other alpha-amylasesAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
InParanoidiP53354.
KOiK01176.
OMAiVSPINEY.
OrthoDBiEOG091G07V6.
PhylomeDBiP53354.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLLVILYS ALVVTNAQLP GFSFGPSGFG GFSLSNPLFN ASFAPANPVD
60 70 80 90 100
RISDIASRFR DVANNVQRNL PKIPQFTNPL SAFSRFARPT SSSGSTNANS
110 120 130 140 150
NNNQNFNSNQ ANEGTSGARN AINEVLNRIP GLSGVPRVPE IPKVPEIPSF
160 170 180 190 200
GNPSDVFGPF VNSLRNMTNQ GINDPGQMFN QVNKFLPDVS RIANSVANYA
210 220 230 240 250
KSAANSGIWK NISNDIKHEM QNRIESMTQM LSNITSAMKN IAQSLPNLMQ
260 270 280 290 300
DQSLNTTLRN QPFFFPGHSG IVHLFEWKFS DIAEECENVL GPNGYGGVQV
310 320 330 340 350
SPINEYLVSP SRAWWERYQP ISFEIKSRSG NEKQFSDMVK RCMKAGVRIY
360 370 380 390 400
VDVVVNHMAA PGASAPLYGT AGSTCDPQAR DYPGVPFNRS HFHADCQIND
410 420 430 440 450
YNNATNVRNC ELAALPDLDQ SNRFVQNKII QYLNHLLDLG VAGFRMDACK
460 470 480 490 500
HMRPEDLKSI YVRLKPVNAM FLFPPGARPF IFQEVIDLGT EGVSAKEYTN
510 520 530 540 550
LGVVTEFNWC IAVGGVFRGT TNADALELLT KNGSAGVLLP SSQALVFVDN
560 570 580 590 600
HDNQRGHGAG GDSILTYKTK RQYIQAVAFT LATDYGIARV MSSYNFSDPD
610 620 630 640 650
QGPPQDTVQV IKSPGFAANN SCSNGWVCEH RWPEIRKMIQ FKNFVAGSSL
660 670 680 690 700
DHIQATQNTF AFCRGEKGFI VFNNSENTIT QQYHTCLPQG QYCDIISGEV
710 720 730
SQSTCTGTVV NVDANGDAEI TLPKNSIVAI YVPSRLS
Length:737
Mass (Da):80,891
Last modified:July 22, 2008 - v2
Checksum:i682A1F42055AC753
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191L → F in AAA29343 (PubMed:7505701).Curated
Sequence conflicti137 – 1371R → H in AAA29343 (PubMed:7505701).Curated
Sequence conflicti158 – 1581G → A in AAA29343 (PubMed:7505701).Curated
Sequence conflicti173 – 1731N → K in AAB60935 (PubMed:9443377).Curated
Sequence conflicti178 – 1781M → I in AAB60935 (PubMed:9443377).Curated
Sequence conflicti178 – 1781M → I in AAA29343 (PubMed:7505701).Curated
Sequence conflicti184 – 1841K → T in AAB60935 (PubMed:9443377).Curated
Sequence conflicti197 – 1971A → D in AAA29343 (PubMed:7505701).Curated
Sequence conflicti230 – 2301M → I in AAB60935 (PubMed:9443377).Curated
Sequence conflicti245 – 2451L → V in AAB60935 (PubMed:9443377).Curated
Sequence conflicti288 – 2881N → K in AAB60935 (PubMed:9443377).Curated
Sequence conflicti288 – 2881N → K in AAA29343 (PubMed:7505701).Curated
Sequence conflicti378 – 3781Q → L in AAB60935 (PubMed:9443377).Curated
Sequence conflicti453 – 4531R → Q in AAA29343 (PubMed:7505701).Curated
Sequence conflicti462 – 4621V → D in AAB60935 (PubMed:9443377).Curated
Sequence conflicti462 – 4621V → D in AAA29343 (PubMed:7505701).Curated
Sequence conflicti512 – 5121A → V in AAB60935 (PubMed:9443377).Curated
Sequence conflicti512 – 5121A → V in AAA29343 (PubMed:7505701).Curated
Sequence conflicti571 – 5711R → P in AAB60935 (PubMed:9443377).Curated
Sequence conflicti571 – 5711R → P in AAA29343 (PubMed:7505701).Curated
Sequence conflicti612 – 6121K → R in AAB60935 (PubMed:9443377).Curated
Sequence conflicti612 – 6121K → R in AAA29343 (PubMed:7505701).Curated
Sequence conflicti617 – 6171A → V in AAB60935 (PubMed:9443377).Curated
Sequence conflicti617 – 6171A → V in AAA29343 (PubMed:7505701).Curated
Sequence conflicti618 – 6181A → V in AAB60935 (PubMed:9443377).Curated
Sequence conflicti624 – 6241N → T in AAB60935 (PubMed:9443377).Curated
Sequence conflicti624 – 6241N → T in AAA29343 (PubMed:7505701).Curated
Sequence conflicti727 – 7271I → V in AAB60935 (PubMed:9443377).Curated
Sequence conflicti727 – 7271I → V in AAA29345 (PubMed:7505701).Curated
Sequence conflicti732 – 7321V → I in AAB60935 (PubMed:9443377).Curated
Sequence conflicti732 – 7321V → I in AAA29345 (PubMed:7505701).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000569 Genomic DNA. Translation: AAB60935.1.
CH477404 Genomic DNA. Translation: EAT41631.1.
L03637 mRNA. Translation: AAA29343.1.
L03638 mRNA. Translation: AAA29344.1.
L03639 Genomic DNA. Translation: AAA29345.1.
L03640 Genomic DNA. Translation: AAA29346.1.
RefSeqiXP_001652172.1. XM_001652122.1.
UniGeneiAae.20631.

Genome annotation databases

EnsemblMetazoaiAAEL006719-RA; AAEL006719-PA; AAEL006719.
GeneIDi5568295.
KEGGiaag:AaeL_AAEL006719.
VectorBaseiAAEL006719-RA; AAEL006719-PA; AAEL006719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000569 Genomic DNA. Translation: AAB60935.1.
CH477404 Genomic DNA. Translation: EAT41631.1.
L03637 mRNA. Translation: AAA29343.1.
L03638 mRNA. Translation: AAA29344.1.
L03639 Genomic DNA. Translation: AAA29345.1.
L03640 Genomic DNA. Translation: AAA29346.1.
RefSeqiXP_001652172.1. XM_001652122.1.
UniGeneiAae.20631.

3D structure databases

ProteinModelPortaliP53354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7159.AAEL006719-PA.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL006719-RA; AAEL006719-PA; AAEL006719.
GeneIDi5568295.
KEGGiaag:AaeL_AAEL006719.
VectorBaseiAAEL006719-RA; AAEL006719-PA; AAEL006719.

Organism-specific databases

CTDi5568295.

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
InParanoidiP53354.
KOiK01176.
OMAiVSPINEY.
OrthoDBiEOG091G07V6.
PhylomeDBiP53354.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_AEDAE
AccessioniPrimary (citable) accession number: P53354
Secondary accession number(s): O02413, Q175A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 22, 2008
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.