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Protein

Alpha-amylase I

Gene

AMY1

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi356CalciumBy similarity1
Metal bindingi408Calcium; via carbonyl oxygenBy similarity1
Metal bindingi417CalciumBy similarity1
Binding sitei445ChlorideBy similarity1
Active sitei447NucleophileBy similarity1
Metal bindingi451Calcium; via carbonyl oxygenBy similarity1
Active sitei484Proton donorBy similarity1
Binding sitei550ChlorideBy similarity1
Sitei552Transition state stabilizerBy similarity1
Binding sitei589ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase I (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
Gene namesi
Name:AMY1
Synonyms:AMY I
ORF Names:AAEL006719
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000000135917 – 737Alpha-amylase IAdd BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi40N-linked (GlcNAc...)Sequence analysis1
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1
Glycosylationi233N-linked (GlcNAc...)Sequence analysis1
Glycosylationi255N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi286 ↔ 342By similarity
Glycosylationi388N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi396 ↔ 410By similarity
Glycosylationi403N-linked (GlcNAc...)Sequence analysis1
Glycosylationi532N-linked (GlcNAc...)Sequence analysis1
Glycosylationi595N-linked (GlcNAc...)Sequence analysis1
Glycosylationi619N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi622 ↔ 628By similarity
Glycosylationi673N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi693 ↔ 705By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed specifically in the proximal-lateral lobes of the adult female salivary gland.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7159.AAEL006719-PA.

Structurei

3D structure databases

ProteinModelPortaliP53354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 260Not found in other alpha-amylasesAdd BLAST244

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
InParanoidiP53354.
KOiK01176.
OMAiVSPINEY.
OrthoDBiEOG091G07V6.
PhylomeDBiP53354.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLLVILYS ALVVTNAQLP GFSFGPSGFG GFSLSNPLFN ASFAPANPVD
60 70 80 90 100
RISDIASRFR DVANNVQRNL PKIPQFTNPL SAFSRFARPT SSSGSTNANS
110 120 130 140 150
NNNQNFNSNQ ANEGTSGARN AINEVLNRIP GLSGVPRVPE IPKVPEIPSF
160 170 180 190 200
GNPSDVFGPF VNSLRNMTNQ GINDPGQMFN QVNKFLPDVS RIANSVANYA
210 220 230 240 250
KSAANSGIWK NISNDIKHEM QNRIESMTQM LSNITSAMKN IAQSLPNLMQ
260 270 280 290 300
DQSLNTTLRN QPFFFPGHSG IVHLFEWKFS DIAEECENVL GPNGYGGVQV
310 320 330 340 350
SPINEYLVSP SRAWWERYQP ISFEIKSRSG NEKQFSDMVK RCMKAGVRIY
360 370 380 390 400
VDVVVNHMAA PGASAPLYGT AGSTCDPQAR DYPGVPFNRS HFHADCQIND
410 420 430 440 450
YNNATNVRNC ELAALPDLDQ SNRFVQNKII QYLNHLLDLG VAGFRMDACK
460 470 480 490 500
HMRPEDLKSI YVRLKPVNAM FLFPPGARPF IFQEVIDLGT EGVSAKEYTN
510 520 530 540 550
LGVVTEFNWC IAVGGVFRGT TNADALELLT KNGSAGVLLP SSQALVFVDN
560 570 580 590 600
HDNQRGHGAG GDSILTYKTK RQYIQAVAFT LATDYGIARV MSSYNFSDPD
610 620 630 640 650
QGPPQDTVQV IKSPGFAANN SCSNGWVCEH RWPEIRKMIQ FKNFVAGSSL
660 670 680 690 700
DHIQATQNTF AFCRGEKGFI VFNNSENTIT QQYHTCLPQG QYCDIISGEV
710 720 730
SQSTCTGTVV NVDANGDAEI TLPKNSIVAI YVPSRLS
Length:737
Mass (Da):80,891
Last modified:July 22, 2008 - v2
Checksum:i682A1F42055AC753
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19L → F in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti137R → H in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti158G → A in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti173N → K in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti178M → I in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti178M → I in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti184K → T in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti197A → D in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti230M → I in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti245L → V in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti288N → K in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti288N → K in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti378Q → L in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti453R → Q in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti462V → D in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti462V → D in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti512A → V in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti512A → V in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti571R → P in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti571R → P in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti612K → R in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti612K → R in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti617A → V in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti617A → V in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti618A → V in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti624N → T in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti624N → T in AAA29343 (PubMed:7505701).Curated1
Sequence conflicti727I → V in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti727I → V in AAA29345 (PubMed:7505701).Curated1
Sequence conflicti732V → I in AAB60935 (PubMed:9443377).Curated1
Sequence conflicti732V → I in AAA29345 (PubMed:7505701).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000569 Genomic DNA. Translation: AAB60935.1.
CH477404 Genomic DNA. Translation: EAT41631.1.
L03637 mRNA. Translation: AAA29343.1.
L03638 mRNA. Translation: AAA29344.1.
L03639 Genomic DNA. Translation: AAA29345.1.
L03640 Genomic DNA. Translation: AAA29346.1.
RefSeqiXP_001652172.1. XM_001652122.1.
UniGeneiAae.20631.

Genome annotation databases

EnsemblMetazoaiAAEL006719-RA; AAEL006719-PA; AAEL006719.
GeneIDi5568295.
KEGGiaag:AaeL_AAEL006719.
VectorBaseiAAEL006719-RA; AAEL006719-PA; AAEL006719.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000569 Genomic DNA. Translation: AAB60935.1.
CH477404 Genomic DNA. Translation: EAT41631.1.
L03637 mRNA. Translation: AAA29343.1.
L03638 mRNA. Translation: AAA29344.1.
L03639 Genomic DNA. Translation: AAA29345.1.
L03640 Genomic DNA. Translation: AAA29346.1.
RefSeqiXP_001652172.1. XM_001652122.1.
UniGeneiAae.20631.

3D structure databases

ProteinModelPortaliP53354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7159.AAEL006719-PA.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL006719-RA; AAEL006719-PA; AAEL006719.
GeneIDi5568295.
KEGGiaag:AaeL_AAEL006719.
VectorBaseiAAEL006719-RA; AAEL006719-PA; AAEL006719.

Organism-specific databases

CTDi5568295.

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
InParanoidiP53354.
KOiK01176.
OMAiVSPINEY.
OrthoDBiEOG091G07V6.
PhylomeDBiP53354.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY1_AEDAE
AccessioniPrimary (citable) accession number: P53354
Secondary accession number(s): O02413, Q175A0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 22, 2008
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.