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P53341

- MAL12_YEAST

UniProt

P53341 - MAL12_YEAST

Protein

Alpha-glucosidase MAL12

Gene

MAL12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei214 – 2141NucleophileBy similarity
    Active sitei276 – 2761Proton donorBy similarity
    Sitei349 – 3491Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. cation binding Source: InterPro
    2. glucan 1,4-alpha-maltotriohydrolase activity Source: SGD
    3. maltose alpha-glucosidase activity Source: SGD
    4. sucrose alpha-glucosidase activity Source: SGD

    GO - Biological processi

    1. maltose catabolic process Source: SGD
    2. sucrose catabolic process Source: SGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Maltose metabolism

    Enzyme and pathway databases

    BioCyciYEAST:YGR292W-MONOMER.

    Protein family/group databases

    CAZyiGH13. Glycoside Hydrolase Family 13.
    mycoCLAPiAGL13L_YEAST.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-glucosidase MAL12 (EC:3.2.1.20)
    Alternative name(s):
    Maltase
    Gene namesi
    Name:MAL12
    Synonyms:MAL1S
    Ordered Locus Names:YGR292W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR292w.
    SGDiS000003524. MAL12.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 584584Alpha-glucosidase MAL12PRO_0000054328Add
    BLAST

    Proteomic databases

    MaxQBiP53341.
    PaxDbiP53341.

    Expressioni

    Gene expression databases

    GenevestigatoriP53341.

    Interactioni

    Protein-protein interaction databases

    BioGridi33540. 71 interactions.
    DIPiDIP-4890N.
    IntActiP53341. 1 interaction.
    MINTiMINT-519330.
    STRINGi4932.YGR292W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53341.
    SMRiP53341. Positions 4-582.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiCOG0366.
    GeneTreeiENSGT00530000063127.
    HOGENOMiHOG000220641.
    KOiK01187.
    OMAiKSAMEFW.
    OrthoDBiEOG7M3J7X.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P53341-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD    50
    LGVDAIWVCP FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG 100
    MKFITDLVIN HCSTEHEWFK ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP 150
    PNNWKSFFGG SAWTFDETTN EFYLRLFASR QVDLNWENED CRRAIFESAV 200
    GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP NWGSHNGPRI 250
    HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF 300
    THVEVGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ 350
    ARSITRFADD SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP 400
    IEKYEDVDVK NNYEIIKKSF GKNSKEMKDF FKGIALLSRD HSRTPMPWTK 450
    DKPNAGFTGP DVKPWFLLNE SFEQGINVEQ ESRDDDSVLN FWKRALQARK 500
    KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF SGEEIEFSLP 550
    REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK 584
    Length:584
    Mass (Da):68,094
    Last modified:October 1, 1996 - v1
    Checksum:iB10FCD5A5E423211
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73077 Genomic DNA. Translation: CAA97325.1.
    BK006941 Genomic DNA. Translation: DAA08378.1.
    PIRiS64627.
    RefSeqiNP_011808.3. NM_001181421.3.

    Genome annotation databases

    EnsemblFungiiYGR292W; YGR292W; YGR292W.
    GeneIDi853209.
    KEGGisce:YGR292W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z73077 Genomic DNA. Translation: CAA97325.1 .
    BK006941 Genomic DNA. Translation: DAA08378.1 .
    PIRi S64627.
    RefSeqi NP_011808.3. NM_001181421.3.

    3D structure databases

    ProteinModelPortali P53341.
    SMRi P53341. Positions 4-582.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33540. 71 interactions.
    DIPi DIP-4890N.
    IntActi P53341. 1 interaction.
    MINTi MINT-519330.
    STRINGi 4932.YGR292W.

    Chemistry

    BindingDBi P53341.
    ChEMBLi CHEMBL2932.

    Protein family/group databases

    CAZyi GH13. Glycoside Hydrolase Family 13.
    mycoCLAPi AGL13L_YEAST.

    Proteomic databases

    MaxQBi P53341.
    PaxDbi P53341.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR292W ; YGR292W ; YGR292W .
    GeneIDi 853209.
    KEGGi sce:YGR292W.

    Organism-specific databases

    CYGDi YGR292w.
    SGDi S000003524. MAL12.

    Phylogenomic databases

    eggNOGi COG0366.
    GeneTreei ENSGT00530000063127.
    HOGENOMi HOG000220641.
    KOi K01187.
    OMAi KSAMEFW.
    OrthoDBi EOG7M3J7X.

    Enzyme and pathway databases

    BioCyci YEAST:YGR292W-MONOMER.

    Miscellaneous databases

    NextBioi 973392.

    Gene expression databases

    Genevestigatori P53341.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
      Volckaert G., Voet M., Robben J.
      Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMAL12_YEAST
    AccessioniPrimary (citable) accession number: P53341
    Secondary accession number(s): D6VV67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5060 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3