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Protein

Alpha-glucosidase MAL12

Gene

MAL12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 5060 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei214NucleophileBy similarity1
Active sitei276Proton donorBy similarity1
Sitei349Transition state stabilizerBy similarity1

GO - Molecular functioni

  • alpha-1,4-glucosidase activity Source: UniProtKB-EC
  • glucan 1,4-alpha-maltotriohydrolase activity Source: SGD
  • maltose alpha-glucosidase activity Source: SGD
  • sucrose alpha-glucosidase activity Source: SGD

GO - Biological processi

  • maltose catabolic process Source: SGD
  • sucrose catabolic process Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processMaltose metabolism

Enzyme and pathway databases

BioCyciYEAST:YGR292W-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiAGL13L_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-glucosidase MAL12 (EC:3.2.1.20)
Alternative name(s):
Maltase
Gene namesi
Name:MAL12
Synonyms:MAL1S
Ordered Locus Names:YGR292W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR292W.
SGDiS000003524. MAL12.

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2932.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000543281 – 584Alpha-glucosidase MAL12Add BLAST584

Proteomic databases

MaxQBiP53341.
PRIDEiP53341.
TopDownProteomicsiP53341.

Interactioni

Protein-protein interaction databases

BioGridi33540. 248 interactors.
DIPiDIP-4890N.
IntActiP53341. 1 interactor.
MINTiMINT-519330.

Chemistry databases

BindingDBiP53341.

Structurei

3D structure databases

ProteinModelPortaliP53341.
SMRiP53341.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

GeneTreeiENSGT00870000137001.
HOGENOMiHOG000220641.
InParanoidiP53341.
KOiK01182.
OMAiQVIPYVS.
OrthoDBiEOG092C12JX.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P53341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD
60 70 80 90 100
LGVDAIWVCP FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG
110 120 130 140 150
MKFITDLVIN HCSTEHEWFK ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP
160 170 180 190 200
PNNWKSFFGG SAWTFDETTN EFYLRLFASR QVDLNWENED CRRAIFESAV
210 220 230 240 250
GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP NWGSHNGPRI
260 270 280 290 300
HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF
310 320 330 340 350
THVEVGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ
360 370 380 390 400
ARSITRFADD SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP
410 420 430 440 450
IEKYEDVDVK NNYEIIKKSF GKNSKEMKDF FKGIALLSRD HSRTPMPWTK
460 470 480 490 500
DKPNAGFTGP DVKPWFLLNE SFEQGINVEQ ESRDDDSVLN FWKRALQARK
510 520 530 540 550
KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF SGEEIEFSLP
560 570 580
REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK
Length:584
Mass (Da):68,094
Last modified:October 1, 1996 - v1
Checksum:iB10FCD5A5E423211
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73077 Genomic DNA. Translation: CAA97325.1.
BK006941 Genomic DNA. Translation: DAA08378.1.
PIRiS64627.
RefSeqiNP_011808.3. NM_001181421.3.

Genome annotation databases

EnsemblFungiiYGR292W; YGR292W; YGR292W.
GeneIDi853209.
KEGGisce:YGR292W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73077 Genomic DNA. Translation: CAA97325.1.
BK006941 Genomic DNA. Translation: DAA08378.1.
PIRiS64627.
RefSeqiNP_011808.3. NM_001181421.3.

3D structure databases

ProteinModelPortaliP53341.
SMRiP53341.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33540. 248 interactors.
DIPiDIP-4890N.
IntActiP53341. 1 interactor.
MINTiMINT-519330.

Chemistry databases

BindingDBiP53341.
ChEMBLiCHEMBL2932.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.
mycoCLAPiAGL13L_YEAST.

Proteomic databases

MaxQBiP53341.
PRIDEiP53341.
TopDownProteomicsiP53341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR292W; YGR292W; YGR292W.
GeneIDi853209.
KEGGisce:YGR292W.

Organism-specific databases

EuPathDBiFungiDB:YGR292W.
SGDiS000003524. MAL12.

Phylogenomic databases

GeneTreeiENSGT00870000137001.
HOGENOMiHOG000220641.
InParanoidiP53341.
KOiK01182.
OMAiQVIPYVS.
OrthoDBiEOG092C12JX.

Enzyme and pathway databases

BioCyciYEAST:YGR292W-MONOMER.
ReactomeiR-SCE-352230. Amino acid transport across the plasma membrane.

Miscellaneous databases

PROiPR:P53341.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiView protein in InterPro
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMAL12_YEAST
AccessioniPrimary (citable) accession number: P53341
Secondary accession number(s): D6VV67
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.