P53341 (MAL12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-glucosidase MAL12
EC=3.2.1.20

Alternative name(s):
Maltase

Gene names

Name:	MAL12
Synonyms:	MAL1S
Ordered Locus Names:	YGR292W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Miscellaneous	Present with 5060 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Maltose metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maltose catabolic process Inferred from genetic interaction PubMed 20471265. Source: SGD sucrose catabolic process Inferred from genetic interaction PubMed 20471265. Source: SGD
Cellular_component	plasma membrane Inferred from direct assay PubMed 16622836. Source: SGD
Molecular_function	cation binding Inferred from electronic annotation. Source: InterPro maltose alpha-glucosidase activity Inferred from direct assay PubMed 20471265. Source: SGD sucrose alpha-glucosidase activity Inferred from direct assay PubMed 20471265. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Alpha-glucosidase MAL12		PRO_0000054328
Sites
	Active site	214	1	Nucleophile By similarity
	Active site	276	1	Proton donor By similarity
	Site	349	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P53341 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B10FCD5A5E423211	FASTA	584	68,094
	10 20 30 40 50 60 MTISDHPETE PKWWKEATIY QIYPASFKDS NNDGWGDLKG ITSKLQYIKD LGVDAIWVCP 70 80 90 100 110 120 FYDSPQQDMG YDISNYEKVW PTYGTNEDCF ELIDKTHKLG MKFITDLVIN HCSTEHEWFK 130 140 150 160 170 180 ESRSSKTNPK RDWFFWRPPK GYDAEGKPIP PNNWKSFFGG SAWTFDETTN EFYLRLFASR 190 200 210 220 230 240 QVDLNWENED CRRAIFESAV GFWLDHGVDG FRIDTAGLYS KRPGLPDSPI FDKTSKLQHP 250 260 270 280 290 300 NWGSHNGPRI HEYHQELHRF MKNRVKDGRE IMTVGEVAHG SDNALYTSAA RYEVSEVFSF 310 320 330 340 350 360 THVEVGTSPF FRYNIVPFTL KQWKEAIASN FLFINGTDSW ATTYIENHDQ ARSITRFADD 370 380 390 400 410 420 SPKYRKISGK LLTLLECSLT GTLYVYQGQE IGQINFKEWP IEKYEDVDVK NNYEIIKKSF 430 440 450 460 470 480 GKNSKEMKDF FKGIALLSRD HSRTPMPWTK DKPNAGFTGP DVKPWFLLNE SFEQGINVEQ 490 500 510 520 530 540 ESRDDDSVLN FWKRALQARK KYKELMIYGY DFQFIDLDSD QIFSFTKEYE DKTLFAALNF 550 560 570 580 SGEEIEFSLP REGASLSFIL GNYDDTDVSS RVLKPWEGRI YLVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene." Volckaert G., Voet M., Robben J. Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73077 Genomic DNA. Translation: CAA97325.1. BK006941 Genomic DNA. Translation: DAA08378.1.
PIR	S64627.
RefSeq	NP_011808.3. NM_001181421.3.
3D structure databases
ProteinModelPortal	P53341.
SMR	P53341. Positions 4-582.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4890N.
IntAct	P53341. 1 interaction.
MINT	MINT-519330.
STRING	4932.YGR292W.
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
mycoCLAP	AGL13L_YEAST.
Proteomic databases
PaxDb	P53341.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR292W; YGR292W; YGR292W.
GeneID	853209.
KEGG	sce:YGR292W.
Organism-specific databases
CYGD	YGR292w.
SGD	S000003524. MAL12.
Phylogenomic databases
eggNOG	COG0366.
GeneTree	ENSGT00530000063127.
HOGENOM	HOG000220641.
KO	K01187.
OMA	WENPQVH.
OrthoDB	EOG4K0TWQ.
Enzyme and pathway databases
BioCyc	YEAST:YGR292W-MONOMER.
Gene expression databases
ArrayExpress	P53341.
Genevestigator	P53341.
GermOnline	YGR292W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
BindingDB	P53341.
ChEMBL	CHEMBL2932.
NextBio	973392.

Entry information

Entry name

MAL12_YEAST

Accession

Primary (citable) accession number: P53341
Secondary accession number(s): D6VV67

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program