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Protein

Pre-mRNA-splicing factor CWC22

Gene

CWC22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30942-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC22
Alternative name(s):
Complexed with CEF1 protein 22
Gene namesi
Name:CWC22
Ordered Locus Names:YGR278W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR278W.
SGDiS000003510. CWC22.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Pre-mRNA-splicing factor CWC22PRO_0000215678Add
BLAST

Proteomic databases

MaxQBiP53333.
PeptideAtlasiP53333.

PTM databases

iPTMnetiP53333.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi33528. 33 interactions.
DIPiDIP-1869N.
IntActiP53333. 15 interactions.
MINTiMINT-408252.

Structurei

3D structure databases

ProteinModelPortaliP53333.
SMRiP53333. Positions 11-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 204183MIF4GPROSITE-ProRule annotationAdd
BLAST
Domaini290 – 407118MIPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi533 – 57745Arg-richAdd
BLAST

Sequence similaritiesi

Belongs to the CWC22 family.Curated
Contains 1 MI domain.PROSITE-ProRule annotation
Contains 1 MIF4G domain.Curated

Phylogenomic databases

GeneTreeiENSGT00650000093379.
HOGENOMiHOG000112091.
InParanoidiP53333.
KOiK13100.
OMAiEHEEAYR.
OrthoDBiEOG71VT2Q.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
[Graphical view]
SMARTiSM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51366. MI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL
60 70 80 90 100
IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPDI GETLAKELML
110 120 130 140 150
MFVQQFNRKD YVSCGNILQC LSILFLYDVI HEIVILQILL LLLEKNSLRL
160 170 180 190 200
VIAVMKICGW KLALVSKKTH DMIWEKLRYI LQTQELSSTL RESLETLFEI
210 220 230 240 250
RQKDYKSGSQ GLFILDPTSY TVHTHSYIVS DEDEANKELG NFEKCENFNE
260 270 280 290 300
LTMAFDTLRQ KLLINNTSDT NEGSNSQLQI YDMTSTNDVE FKKKIYLVLK
310 320 330 340 350
SSLSGDEAAH KLLKLKIANN LKKSVVDIII KSSLQESTFS KFYSILSERM
360 370 380 390 400
ITFHRSWQTA YNETFEQNYT QDIEDYETDQ LRILGKFWGH LISYEFLPMD
410 420 430 440 450
CLKIIKLTEE ESCPQGRIFI KFLFQELVNE LGLDELQLRL NSSKLDGMFP
460 470 480 490 500
LEGDAEHIRY SINFFTAIGL GLLTEDMRSR LTIIQEVEDA EEEEKKLREE
510 520 530 540 550
EELEKLRKKA RESQPTQGPK IHESRLFLQK DTRENSRSRS PFTVETRKRA
560 570
RSRTPPRGSR NHRNRSRTPP ARRQRHR
Length:577
Mass (Da):67,294
Last modified:October 1, 1996 - v1
Checksum:i773E33C71E4A29D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73063 Genomic DNA. Translation: CAA97309.1.
BK006941 Genomic DNA. Translation: DAA08366.1.
PIRiS64613.
RefSeqiNP_011794.3. NM_001181407.3.

Genome annotation databases

EnsemblFungiiYGR278W; YGR278W; YGR278W.
GeneIDi853195.
KEGGisce:YGR278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73063 Genomic DNA. Translation: CAA97309.1.
BK006941 Genomic DNA. Translation: DAA08366.1.
PIRiS64613.
RefSeqiNP_011794.3. NM_001181407.3.

3D structure databases

ProteinModelPortaliP53333.
SMRiP53333. Positions 11-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33528. 33 interactions.
DIPiDIP-1869N.
IntActiP53333. 15 interactions.
MINTiMINT-408252.

PTM databases

iPTMnetiP53333.

Proteomic databases

MaxQBiP53333.
PeptideAtlasiP53333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR278W; YGR278W; YGR278W.
GeneIDi853195.
KEGGisce:YGR278W.

Organism-specific databases

EuPathDBiFungiDB:YGR278W.
SGDiS000003510. CWC22.

Phylogenomic databases

GeneTreeiENSGT00650000093379.
HOGENOMiHOG000112091.
InParanoidiP53333.
KOiK13100.
OMAiEHEEAYR.
OrthoDBiEOG71VT2Q.

Enzyme and pathway databases

BioCyciYEAST:G3O-30942-MONOMER.

Miscellaneous databases

NextBioi973356.
PROiP53333.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
[Graphical view]
SMARTiSM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51366. MI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
    Volckaert G., Voet M., Robben J.
    Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs."
    Ohi M.D., Link A.J., Ren L., Jennings J.L., McDonald W.H., Gould K.L.
    Mol. Cell. Biol. 22:2011-2024(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CWC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCWC22_YEAST
AccessioniPrimary (citable) accession number: P53333
Secondary accession number(s): D6VV55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.