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Protein

Pre-mRNA-splicing factor CWC22

Gene

CWC22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30942-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC22
Alternative name(s):
Complexed with CEF1 protein 22
Gene namesi
Name:CWC22
Ordered Locus Names:YGR278W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR278W.
SGDiS000003510. CWC22.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • cytoplasm Source: UniProtKB-SubCell
  • U2-type spliceosomal complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002156781 – 577Pre-mRNA-splicing factor CWC22Add BLAST577

Proteomic databases

MaxQBiP53333.
PRIDEiP53333.

PTM databases

iPTMnetiP53333.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi33528. 33 interactors.
DIPiDIP-1869N.
IntActiP53333. 15 interactors.
MINTiMINT-408252.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50Z1-577[»]
5GMKelectron microscopy3.40Z1-577[»]
5LQWelectron microscopy5.80H1-577[»]
ProteinModelPortaliP53333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 204MIF4GPROSITE-ProRule annotationAdd BLAST183
Domaini290 – 407MIPROSITE-ProRule annotationAdd BLAST118

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi533 – 577Arg-richAdd BLAST45

Sequence similaritiesi

Belongs to the CWC22 family.Curated
Contains 1 MI domain.PROSITE-ProRule annotation
Contains 1 MIF4G domain.Curated

Phylogenomic databases

GeneTreeiENSGT00650000093379.
HOGENOMiHOG000112091.
InParanoidiP53333.
KOiK13100.
OMAiEHEEAYR.
OrthoDBiEOG092C3ZLI.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
[Graphical view]
SMARTiSM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51366. MI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL
60 70 80 90 100
IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPDI GETLAKELML
110 120 130 140 150
MFVQQFNRKD YVSCGNILQC LSILFLYDVI HEIVILQILL LLLEKNSLRL
160 170 180 190 200
VIAVMKICGW KLALVSKKTH DMIWEKLRYI LQTQELSSTL RESLETLFEI
210 220 230 240 250
RQKDYKSGSQ GLFILDPTSY TVHTHSYIVS DEDEANKELG NFEKCENFNE
260 270 280 290 300
LTMAFDTLRQ KLLINNTSDT NEGSNSQLQI YDMTSTNDVE FKKKIYLVLK
310 320 330 340 350
SSLSGDEAAH KLLKLKIANN LKKSVVDIII KSSLQESTFS KFYSILSERM
360 370 380 390 400
ITFHRSWQTA YNETFEQNYT QDIEDYETDQ LRILGKFWGH LISYEFLPMD
410 420 430 440 450
CLKIIKLTEE ESCPQGRIFI KFLFQELVNE LGLDELQLRL NSSKLDGMFP
460 470 480 490 500
LEGDAEHIRY SINFFTAIGL GLLTEDMRSR LTIIQEVEDA EEEEKKLREE
510 520 530 540 550
EELEKLRKKA RESQPTQGPK IHESRLFLQK DTRENSRSRS PFTVETRKRA
560 570
RSRTPPRGSR NHRNRSRTPP ARRQRHR
Length:577
Mass (Da):67,294
Last modified:October 1, 1996 - v1
Checksum:i773E33C71E4A29D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73063 Genomic DNA. Translation: CAA97309.1.
BK006941 Genomic DNA. Translation: DAA08366.1.
PIRiS64613.
RefSeqiNP_011794.3. NM_001181407.3.

Genome annotation databases

EnsemblFungiiYGR278W; YGR278W; YGR278W.
GeneIDi853195.
KEGGisce:YGR278W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73063 Genomic DNA. Translation: CAA97309.1.
BK006941 Genomic DNA. Translation: DAA08366.1.
PIRiS64613.
RefSeqiNP_011794.3. NM_001181407.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50Z1-577[»]
5GMKelectron microscopy3.40Z1-577[»]
5LQWelectron microscopy5.80H1-577[»]
ProteinModelPortaliP53333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33528. 33 interactors.
DIPiDIP-1869N.
IntActiP53333. 15 interactors.
MINTiMINT-408252.

PTM databases

iPTMnetiP53333.

Proteomic databases

MaxQBiP53333.
PRIDEiP53333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR278W; YGR278W; YGR278W.
GeneIDi853195.
KEGGisce:YGR278W.

Organism-specific databases

EuPathDBiFungiDB:YGR278W.
SGDiS000003510. CWC22.

Phylogenomic databases

GeneTreeiENSGT00650000093379.
HOGENOMiHOG000112091.
InParanoidiP53333.
KOiK13100.
OMAiEHEEAYR.
OrthoDBiEOG092C3ZLI.

Enzyme and pathway databases

BioCyciYEAST:G3O-30942-MONOMER.

Miscellaneous databases

PROiP53333.

Family and domain databases

Gene3Di1.25.40.180. 1 hit.
InterProiIPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR016021. MIF4-like.
IPR003890. MIF4G-like_typ-3.
[Graphical view]
PfamiPF02847. MA3. 1 hit.
[Graphical view]
SMARTiSM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51366. MI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCWC22_YEAST
AccessioniPrimary (citable) accession number: P53333
Secondary accession number(s): D6VV55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3260 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.