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Protein

Pre-mRNA-splicing factor CWC22

Gene

CWC22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

Miscellaneous

Present with 3260 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30942-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC22
Alternative name(s):
Complexed with CEF1 protein 22
Gene namesi
Name:CWC22
Ordered Locus Names:YGR278W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR278W.
SGDiS000003510. CWC22.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002156781 – 577Pre-mRNA-splicing factor CWC22Add BLAST577

Proteomic databases

MaxQBiP53333.
PRIDEiP53333.

PTM databases

iPTMnetiP53333.

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33528. 159 interactors.
DIPiDIP-1869N.
IntActiP53333. 29 interactors.
MINTiMINT-408252.
STRINGi4932.YGR278W.

Structurei

Secondary structure

1577
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 30Combined sources22
Turni34 – 36Combined sources3
Helixi37 – 46Combined sources10
Turni49 – 52Combined sources4
Helixi53 – 65Combined sources13
Helixi73 – 86Combined sources14
Helixi88 – 108Combined sources21
Helixi111 – 126Combined sources16
Helixi132 – 142Combined sources11
Helixi148 – 165Combined sources18
Helixi167 – 183Combined sources17
Helixi188 – 203Combined sources16
Turni217 – 219Combined sources3
Helixi237 – 240Combined sources4
Helixi286 – 300Combined sources15
Beta strandi301 – 303Combined sources3
Turni304 – 307Combined sources4
Helixi308 – 314Combined sources7
Turni319 – 323Combined sources5
Helixi324 – 335Combined sources12
Beta strandi336 – 338Combined sources3
Helixi341 – 351Combined sources11
Helixi355 – 370Combined sources16
Helixi373 – 375Combined sources3
Helixi380 – 393Combined sources14
Helixi399 – 404Combined sources6
Beta strandi409 – 411Combined sources3
Helixi414 – 431Combined sources18
Helixi434 – 442Combined sources9
Beta strandi447 – 449Combined sources3
Helixi455 – 468Combined sources14
Helixi475 – 484Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50Z1-577[»]
5GMKelectron microscopy3.40Z1-577[»]
5LQWelectron microscopy5.80H1-577[»]
5MPSelectron microscopy3.85H1-577[»]
5MQ0electron microscopy4.17H1-577[»]
5WSGelectron microscopy4.00Z1-577[»]
ProteinModelPortaliP53333.
SMRiP53333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 204MIF4GPROSITE-ProRule annotationAdd BLAST183
Domaini290 – 407MIPROSITE-ProRule annotationAdd BLAST118

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi533 – 577Arg-richAdd BLAST45

Sequence similaritiesi

Belongs to the CWC22 family.Curated

Phylogenomic databases

GeneTreeiENSGT00650000093379.
HOGENOMiHOG000112091.
InParanoidiP53333.
KOiK13100.
OMAiYFTSIGM.
OrthoDBiEOG092C3ZLI.

Family and domain databases

InterProiView protein in InterPro
IPR016024. ARM-type_fold.
IPR003891. Initiation_fac_eIF4g_MI.
IPR003890. MIF4G-like_typ-3.
PfamiView protein in Pfam
PF02847. MA3. 1 hit.
SMARTiView protein in SMART
SM00544. MA3. 1 hit.
SM00543. MIF4G. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS51366. MI. 1 hit.

Sequencei

Sequence statusi: Complete.

P53333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL
60 70 80 90 100
IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPDI GETLAKELML
110 120 130 140 150
MFVQQFNRKD YVSCGNILQC LSILFLYDVI HEIVILQILL LLLEKNSLRL
160 170 180 190 200
VIAVMKICGW KLALVSKKTH DMIWEKLRYI LQTQELSSTL RESLETLFEI
210 220 230 240 250
RQKDYKSGSQ GLFILDPTSY TVHTHSYIVS DEDEANKELG NFEKCENFNE
260 270 280 290 300
LTMAFDTLRQ KLLINNTSDT NEGSNSQLQI YDMTSTNDVE FKKKIYLVLK
310 320 330 340 350
SSLSGDEAAH KLLKLKIANN LKKSVVDIII KSSLQESTFS KFYSILSERM
360 370 380 390 400
ITFHRSWQTA YNETFEQNYT QDIEDYETDQ LRILGKFWGH LISYEFLPMD
410 420 430 440 450
CLKIIKLTEE ESCPQGRIFI KFLFQELVNE LGLDELQLRL NSSKLDGMFP
460 470 480 490 500
LEGDAEHIRY SINFFTAIGL GLLTEDMRSR LTIIQEVEDA EEEEKKLREE
510 520 530 540 550
EELEKLRKKA RESQPTQGPK IHESRLFLQK DTRENSRSRS PFTVETRKRA
560 570
RSRTPPRGSR NHRNRSRTPP ARRQRHR
Length:577
Mass (Da):67,294
Last modified:October 1, 1996 - v1
Checksum:i773E33C71E4A29D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73063 Genomic DNA. Translation: CAA97309.1.
BK006941 Genomic DNA. Translation: DAA08366.1.
PIRiS64613.
RefSeqiNP_011794.3. NM_001181407.3.

Genome annotation databases

EnsemblFungiiYGR278W; YGR278W; YGR278W.
GeneIDi853195.
KEGGisce:YGR278W.

Similar proteinsi

Entry informationi

Entry nameiCWC22_YEAST
AccessioniPrimary (citable) accession number: P53333
Secondary accession number(s): D6VV55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names