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Protein

Pre-mRNA-splicing factor CWC22

Gene

CWC22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in pre-mRNA splicing.

Miscellaneous

Present with 3260 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA splicing, via spliceosome Source: SGD

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

BioCyciYEAST:G3O-30942-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor CWC22
Alternative name(s):
Complexed with CEF1 protein 22
Gene namesi
Name:CWC22
Ordered Locus Names:YGR278W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR278W
SGDiS000003510 CWC22

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002156781 – 577Pre-mRNA-splicing factor CWC22Add BLAST577

Proteomic databases

MaxQBiP53333
PaxDbiP53333
PRIDEiP53333

PTM databases

iPTMnetiP53333

Interactioni

Subunit structurei

Belongs to the CWC complex (or CEF1-associated complex), a spliceosome subcomplex composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.1 Publication

Protein-protein interaction databases

BioGridi33528, 159 interactors
DIPiDIP-1869N
IntActiP53333, 19 interactors
MINTiP53333
STRINGi4932.YGR278W

Structurei

Secondary structure

1577
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 30Combined sources16
Turni34 – 36Combined sources3
Helixi37 – 44Combined sources8
Turni49 – 52Combined sources4
Helixi53 – 64Combined sources12
Helixi72 – 86Combined sources15
Helixi88 – 107Combined sources20
Helixi111 – 126Combined sources16
Helixi134 – 143Combined sources10
Turni144 – 146Combined sources3
Helixi151 – 165Combined sources15
Helixi167 – 182Combined sources16
Helixi188 – 201Combined sources14
Turni217 – 219Combined sources3
Helixi237 – 240Combined sources4
Turni246 – 249Combined sources4
Helixi250 – 261Combined sources12
Helixi289 – 300Combined sources12
Beta strandi301 – 303Combined sources3
Helixi305 – 315Combined sources11
Helixi322 – 335Combined sources14
Beta strandi336 – 338Combined sources3
Helixi342 – 351Combined sources10
Helixi355 – 370Combined sources16
Helixi372 – 375Combined sources4
Helixi378 – 393Combined sources16
Helixi399 – 404Combined sources6
Turni409 – 411Combined sources3
Helixi414 – 430Combined sources17
Helixi435 – 443Combined sources9
Turni446 – 448Combined sources3
Helixi455 – 468Combined sources14
Turni472 – 474Combined sources3
Helixi475 – 485Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GM6electron microscopy3.50Z1-577[»]
5GMKelectron microscopy3.40Z1-577[»]
5LQWelectron microscopy5.80H1-577[»]
5MPSelectron microscopy3.85H1-577[»]
5MQ0electron microscopy4.17H1-577[»]
5WSGelectron microscopy4.00Z1-577[»]
6BK8electron microscopy3.30L1-577[»]
6EXNelectron microscopy3.70H1-577[»]
ProteinModelPortaliP53333
SMRiP53333
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 204MIF4GPROSITE-ProRule annotationAdd BLAST183
Domaini290 – 407MIPROSITE-ProRule annotationAdd BLAST118

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi533 – 577Arg-richAdd BLAST45

Sequence similaritiesi

Belongs to the CWC22 family.Curated

Phylogenomic databases

GeneTreeiENSGT00650000093379
HOGENOMiHOG000112091
InParanoidiP53333
KOiK13100
OMAiQTNDRKQ
OrthoDBiEOG092C3ZLI

Family and domain databases

Gene3Di1.25.40.180, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
PfamiView protein in Pfam
PF02847 MA3, 1 hit
SMARTiView protein in SMART
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit

Sequencei

Sequence statusi: Complete.

P53333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTATIQDED IKFQRENWEM IRSHVSPIIS NLTMDNLQES HRDLFQVNIL
60 70 80 90 100
IGRNIICKNV VDFTLNKQNG RLIPALSALI ALLNSDIPDI GETLAKELML
110 120 130 140 150
MFVQQFNRKD YVSCGNILQC LSILFLYDVI HEIVILQILL LLLEKNSLRL
160 170 180 190 200
VIAVMKICGW KLALVSKKTH DMIWEKLRYI LQTQELSSTL RESLETLFEI
210 220 230 240 250
RQKDYKSGSQ GLFILDPTSY TVHTHSYIVS DEDEANKELG NFEKCENFNE
260 270 280 290 300
LTMAFDTLRQ KLLINNTSDT NEGSNSQLQI YDMTSTNDVE FKKKIYLVLK
310 320 330 340 350
SSLSGDEAAH KLLKLKIANN LKKSVVDIII KSSLQESTFS KFYSILSERM
360 370 380 390 400
ITFHRSWQTA YNETFEQNYT QDIEDYETDQ LRILGKFWGH LISYEFLPMD
410 420 430 440 450
CLKIIKLTEE ESCPQGRIFI KFLFQELVNE LGLDELQLRL NSSKLDGMFP
460 470 480 490 500
LEGDAEHIRY SINFFTAIGL GLLTEDMRSR LTIIQEVEDA EEEEKKLREE
510 520 530 540 550
EELEKLRKKA RESQPTQGPK IHESRLFLQK DTRENSRSRS PFTVETRKRA
560 570
RSRTPPRGSR NHRNRSRTPP ARRQRHR
Length:577
Mass (Da):67,294
Last modified:October 1, 1996 - v1
Checksum:i773E33C71E4A29D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73063 Genomic DNA Translation: CAA97309.1
BK006941 Genomic DNA Translation: DAA08366.1
PIRiS64613
RefSeqiNP_011794.3, NM_001181407.3

Genome annotation databases

EnsemblFungiiYGR278W; YGR278W; YGR278W
GeneIDi853195
KEGGisce:YGR278W

Similar proteinsi

Entry informationi

Entry nameiCWC22_YEAST
AccessioniPrimary (citable) accession number: P53333
Secondary accession number(s): D6VV55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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