Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphopantetheine adenylyltransferase

Gene

CAB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity). Plays a role in the physiological regulation of the intracellular CoA concentration.By similarity1 Publication

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • dephospho-CoA kinase activity Source: GO_Central
  • pantetheine-phosphate adenylyltransferase activity Source: SGD

GO - Biological processi

  • coenzyme A biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-487.
YEAST:MONOMER3O-487.
ReactomeiR-SCE-196783. Coenzyme A biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferase (EC:2.7.7.3)
Short name:
PPAT
Alternative name(s):
Coenzyme A biosynthesis protein 4
Dephospho-CoA pyrophosphorylase
Pantetheine-phosphate adenylyltransferase
Gene namesi
Name:CAB4
Ordered Locus Names:YGR277C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR277C.
SGDiS000003509. CAB4.

Subcellular locationi

GO - Cellular componenti

  • CoA-synthesizing protein complex Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002028701 – 305Phosphopantetheine adenylyltransferaseAdd BLAST305

Proteomic databases

MaxQBiP53332.
PRIDEiP53332.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAB2P405066EBI-23648,EBI-25089
CAB3P360765EBI-23648,EBI-26778
CAB5Q039416EBI-23648,EBI-22174

Protein-protein interaction databases

BioGridi33527. 12 interactors.
DIPiDIP-4904N.
IntActiP53332. 5 interactors.
MINTiMINT-528153.

Structurei

3D structure databases

ProteinModelPortaliP53332.
SMRiP53332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic CoaD family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075078.
InParanoidiP53332.
KOiK02201.
OMAiDERCDNV.
OrthoDBiEOG092C09SX.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEENSRVLI VLPYTPPSAT LQRIIGQTIP FLRECQSQLD IVIVPEFKTS
60 70 80 90 100
FQLDSALGKM YSITRDVLLG YGMINSGINI IFNNIHFVES NLQWKVVLLP
110 120 130 140 150
QESTFETWKL ELGQGQYHSI EHYALHDNIM EEIEGPKDAN KFHVTALGGT
160 170 180 190 200
FDHIHDGHKI LLSVSTFITS QRLICGITCD ELLQNKKYKE LIEPYDTRCR
210 220 230 240 250
HVHQFIKLLK PDLSVELVPL RDVCGPTGKV PEIECLVVSR ETVSGAETVN
260 270 280 290 300
KTRIEKGMSP LAVHVVNVLG GREEDGWSEK LSSTEIRRLL KSSASPTCTP

QNPCV
Length:305
Mass (Da):34,307
Last modified:October 1, 1996 - v1
Checksum:iBEB43BEFC54D123C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73062 Genomic DNA. Translation: CAA97307.1.
AY692609 Genomic DNA. Translation: AAT92628.1.
BK006941 Genomic DNA. Translation: DAA08365.1.
PIRiS64612.
RefSeqiNP_011793.3. NM_001181406.3.

Genome annotation databases

EnsemblFungiiYGR277C; YGR277C; YGR277C.
GeneIDi853194.
KEGGisce:YGR277C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73062 Genomic DNA. Translation: CAA97307.1.
AY692609 Genomic DNA. Translation: AAT92628.1.
BK006941 Genomic DNA. Translation: DAA08365.1.
PIRiS64612.
RefSeqiNP_011793.3. NM_001181406.3.

3D structure databases

ProteinModelPortaliP53332.
SMRiP53332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33527. 12 interactors.
DIPiDIP-4904N.
IntActiP53332. 5 interactors.
MINTiMINT-528153.

Proteomic databases

MaxQBiP53332.
PRIDEiP53332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR277C; YGR277C; YGR277C.
GeneIDi853194.
KEGGisce:YGR277C.

Organism-specific databases

EuPathDBiFungiDB:YGR277C.
SGDiS000003509. CAB4.

Phylogenomic databases

GeneTreeiENSGT00550000075078.
InParanoidiP53332.
KOiK02201.
OMAiDERCDNV.
OrthoDBiEOG092C09SX.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-487.
YEAST:MONOMER3O-487.
ReactomeiR-SCE-196783. Coenzyme A biosynthesis.

Miscellaneous databases

PROiP53332.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCOAD_YEAST
AccessioniPrimary (citable) accession number: P53332
Secondary accession number(s): D6VV54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.