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Protein

Phosphopantetheine adenylyltransferase

Gene

CAB4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate (By similarity). Plays a role in the physiological regulation of the intracellular CoA concentration.By similarity1 Publication

Catalytic activityi

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • pantetheine-phosphate adenylyltransferase activity Source: SGD

GO - Biological processi

  • coenzyme A biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-487.
YEAST:MONOMER3O-487.
ReactomeiR-SCE-196783. Coenzyme A biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantetheine adenylyltransferase (EC:2.7.7.3)
Short name:
PPAT
Alternative name(s):
Coenzyme A biosynthesis protein 4
Dephospho-CoA pyrophosphorylase
Pantetheine-phosphate adenylyltransferase
Gene namesi
Name:CAB4
Ordered Locus Names:YGR277C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR277C.
SGDiS000003509. CAB4.

Subcellular locationi

GO - Cellular componenti

  • CoA-synthesizing protein complex Source: SGD
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Phosphopantetheine adenylyltransferasePRO_0000202870Add
BLAST

Proteomic databases

MaxQBiP53332.
PeptideAtlasiP53332.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KES1P358441EBI-23648,EBI-9648

Protein-protein interaction databases

BioGridi33527. 12 interactions.
DIPiDIP-4904N.
IntActiP53332. 2 interactions.
MINTiMINT-528153.

Structurei

3D structure databases

ProteinModelPortaliP53332.
SMRiP53332. Positions 146-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic CoaD family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075078.
InParanoidiP53332.
KOiK02201.
OMAiRDVCGPT.
OrthoDBiEOG7TQV9M.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEENSRVLI VLPYTPPSAT LQRIIGQTIP FLRECQSQLD IVIVPEFKTS
60 70 80 90 100
FQLDSALGKM YSITRDVLLG YGMINSGINI IFNNIHFVES NLQWKVVLLP
110 120 130 140 150
QESTFETWKL ELGQGQYHSI EHYALHDNIM EEIEGPKDAN KFHVTALGGT
160 170 180 190 200
FDHIHDGHKI LLSVSTFITS QRLICGITCD ELLQNKKYKE LIEPYDTRCR
210 220 230 240 250
HVHQFIKLLK PDLSVELVPL RDVCGPTGKV PEIECLVVSR ETVSGAETVN
260 270 280 290 300
KTRIEKGMSP LAVHVVNVLG GREEDGWSEK LSSTEIRRLL KSSASPTCTP

QNPCV
Length:305
Mass (Da):34,307
Last modified:October 1, 1996 - v1
Checksum:iBEB43BEFC54D123C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73062 Genomic DNA. Translation: CAA97307.1.
AY692609 Genomic DNA. Translation: AAT92628.1.
BK006941 Genomic DNA. Translation: DAA08365.1.
PIRiS64612.
RefSeqiNP_011793.3. NM_001181406.3.

Genome annotation databases

EnsemblFungiiYGR277C; YGR277C; YGR277C.
GeneIDi853194.
KEGGisce:YGR277C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z73062 Genomic DNA. Translation: CAA97307.1.
AY692609 Genomic DNA. Translation: AAT92628.1.
BK006941 Genomic DNA. Translation: DAA08365.1.
PIRiS64612.
RefSeqiNP_011793.3. NM_001181406.3.

3D structure databases

ProteinModelPortaliP53332.
SMRiP53332. Positions 146-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33527. 12 interactions.
DIPiDIP-4904N.
IntActiP53332. 2 interactions.
MINTiMINT-528153.

Proteomic databases

MaxQBiP53332.
PeptideAtlasiP53332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR277C; YGR277C; YGR277C.
GeneIDi853194.
KEGGisce:YGR277C.

Organism-specific databases

EuPathDBiFungiDB:YGR277C.
SGDiS000003509. CAB4.

Phylogenomic databases

GeneTreeiENSGT00550000075078.
InParanoidiP53332.
KOiK02201.
OMAiRDVCGPT.
OrthoDBiEOG7TQV9M.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-487.
YEAST:MONOMER3O-487.
ReactomeiR-SCE-196783. Coenzyme A biosynthesis.

Miscellaneous databases

NextBioi973353.
PROiP53332.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR004821. Cyt_trans-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2 genes and an ABC transporter gene."
    Volckaert G., Voet M., Robben J.
    Yeast 13:251-259(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
    Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
    Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-305.
    Strain: ATCC 204508 / S288c.
  6. Cited for: SUBCELLULAR LOCATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces cerevisiae: identification of a conditional mutation in the pantothenate kinase gene CAB1."
    Olzhausen J., Schuebbe S., Schueller H.-J.
    Curr. Genet. 55:163-173(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCOAD_YEAST
AccessioniPrimary (citable) accession number: P53332
Secondary accession number(s): D6VV54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.