SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P53330

- RT102_YEAST

UniProt

P53330 - RT102_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Regulator of Ty1 transposition protein 102
Gene
RTT102, YGR275W, G9378
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Probable component of the chromatin structure-remodeling complex (RSC) which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Probable component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. chromatin remodeling Source: SGD
  3. chromosome segregation Source: SGD
  4. nucleosome disassembly Source: SGD
  5. regulation of transcription, DNA-templated Source: UniProtKB-KW
  6. transcription elongation from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30940-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of Ty1 transposition protein 102
Gene namesi
Name:RTT102
Ordered Locus Names:YGR275W
ORF Names:G9378
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR275w.
SGDiS000003507. RTT102.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. RSC complex Source: SGD
  2. SWI/SNF complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Regulator of Ty1 transposition protein 102
PRO_0000097501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei77 – 771Phosphoserine1 Publication
Modified residuei122 – 1221Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP53330.
PaxDbiP53330.
PeptideAtlasiP53330.

Expressioni

Gene expression databases

GenevestigatoriP53330.

Interactioni

Subunit structurei

Interacts with STH1 and SWI3. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Probable additional component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
STH1P325975EBI-23637,EBI-18410
SWI3P325912EBI-23637,EBI-18622

Protein-protein interaction databases

BioGridi33525. 74 interactions.
DIPiDIP-3864N.
IntActiP53330. 55 interactions.
MINTiMINT-522104.
STRINGi4932.YGR275W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 97
Beta strandi25 – 306
Beta strandi60 – 656
Beta strandi86 – 883

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4I6MX-ray2.80D1-157[»]
ProteinModelPortaliP53330.
SMRiP53330. Positions 1-90.

Family & Domainsi

Phylogenomic databases

eggNOGiNOG76281.
OMAiHEEISAT.
OrthoDBiEOG7RZ63Q.

Family and domain databases

InterProiIPR018304. Tscrpt_reg_Rtt102p.
[Graphical view]
PfamiPF09510. Rtt102p. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53330-1 [UniParc]FASTAAdd to Basket

« Hide

MDPQTLITKA NKVSYYGNPT SKESWRYDWY QPSKVSSNVQ QPQQQLGDME    50
NNLEKYPFRY KTWLRNQEDE KNLQRESCED ILDLKEFDRR ILKKSLMTSH 100
TKGDTSKATG APSANQGDEA LSVDDIRGAV GNSEAIPGLS AGVNNDNTKE 150
SKDVKMN 157
Length:157
Mass (Da):17,795
Last modified:September 13, 2005 - v2
Checksum:iD71CA1234D0D0176
GO

Sequence cautioni

The sequence AAS56708.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA58897.1 differs from that shown. Reason: Erroneous initiation.
The sequence CAA97305.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84098 Genomic DNA. Translation: CAA58897.1. Different initiation.
Z73060 Genomic DNA. Translation: CAA97305.1. Different initiation.
AY558382 Genomic DNA. Translation: AAS56708.1. Different initiation.
BK006941 Genomic DNA. Translation: DAA08363.1.
PIRiS64608.
RefSeqiNP_011791.4. NM_001181404.3.

Genome annotation databases

EnsemblFungiiYGR275W; YGR275W; YGR275W.
GeneIDi853192.
KEGGisce:YGR275W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84098 Genomic DNA. Translation: CAA58897.1 . Different initiation.
Z73060 Genomic DNA. Translation: CAA97305.1 . Different initiation.
AY558382 Genomic DNA. Translation: AAS56708.1 . Different initiation.
BK006941 Genomic DNA. Translation: DAA08363.1 .
PIRi S64608.
RefSeqi NP_011791.4. NM_001181404.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4I6M X-ray 2.80 D 1-157 [» ]
ProteinModelPortali P53330.
SMRi P53330. Positions 1-90.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33525. 74 interactions.
DIPi DIP-3864N.
IntActi P53330. 55 interactions.
MINTi MINT-522104.
STRINGi 4932.YGR275W.

Proteomic databases

MaxQBi P53330.
PaxDbi P53330.
PeptideAtlasi P53330.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR275W ; YGR275W ; YGR275W .
GeneIDi 853192.
KEGGi sce:YGR275W.

Organism-specific databases

CYGDi YGR275w.
SGDi S000003507. RTT102.

Phylogenomic databases

eggNOGi NOG76281.
OMAi HEEISAT.
OrthoDBi EOG7RZ63Q.

Enzyme and pathway databases

BioCyci YEAST:G3O-30940-MONOMER.

Miscellaneous databases

NextBioi 973347.

Gene expression databases

Genevestigatori P53330.

Family and domain databases

InterProi IPR018304. Tscrpt_reg_Rtt102p.
[Graphical view ]
Pfami PF09510. Rtt102p. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
    Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
    Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance."
    Scholes D.T., Banerjee M., Bowen B., Curcio M.J.
    Genetics 159:1449-1465(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
    Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
    Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
  10. "Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits."
    Lee K.K., Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
    Biochem. Soc. Trans. 32:899-903(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH STH1 AND SWI3, IDENTIFICATION IN THE SWI/SNF COMPLEX.
  11. Cited for: IDENTIFICATION IN THE RSC COMPLEX.
  12. "The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion."
    Baetz K.K., Krogan N.J., Emili A., Greenblatt J., Hieter P.
    Mol. Cell. Biol. 24:1232-1244(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE RSC COMPLEX.
  13. "Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast."
    Graumann J., Dunipace L.A., Seol J.H., McDonald W.H., Yates J.R. III, Wold B.J., Deshaies R.J.
    Mol. Cell. Proteomics 3:226-237(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SWI/SNF COMPLEX.
  14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRT102_YEAST
AccessioniPrimary (citable) accession number: P53330
Secondary accession number(s): D6VV52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi