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P53330 (RT102_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of Ty1 transposition protein 102
Gene names
Name:RTT102
Ordered Locus Names:YGR275W
ORF Names:G9378
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length157 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable component of the chromatin structure-remodeling complex (RSC) which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Probable component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.

Subunit structure

Interacts with STH1 and SWI3. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Probable additional component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29. Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location

Nucleus Ref.7.

Miscellaneous

Present with 1550 molecules/cell in log phase SD medium.

Sequence caution

The sequence AAS56708.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA58897.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA97305.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

STH1P325975EBI-23637,EBI-18410
SWI3P325912EBI-23637,EBI-18622

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 157157Regulator of Ty1 transposition protein 102
PRO_0000097501

Amino acid modifications

Modified residue771Phosphoserine Ref.15
Modified residue1221Phosphoserine Ref.14 Ref.15

Secondary structure

......... 157
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P53330 [UniParc].

Last modified September 13, 2005. Version 2.
Checksum: D71CA1234D0D0176

FASTA15717,795
        10         20         30         40         50         60 
MDPQTLITKA NKVSYYGNPT SKESWRYDWY QPSKVSSNVQ QPQQQLGDME NNLEKYPFRY 

        70         80         90        100        110        120 
KTWLRNQEDE KNLQRESCED ILDLKEFDRR ILKKSLMTSH TKGDTSKATG APSANQGDEA 

       130        140        150 
LSVDDIRGAV GNSEAIPGLS AGVNNDNTKE SKDVKMN 

« Hide

References

« Hide 'large scale' references
[1]"The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance."
Scholes D.T., Banerjee M., Bowen B., Curcio M.J.
Genetics 159:1449-1465(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[6]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[10]"Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits."
Lee K.K., Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
Biochem. Soc. Trans. 32:899-903(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH STH1 AND SWI3, IDENTIFICATION IN THE SWI/SNF COMPLEX.
[11]"High-definition macromolecular composition of yeast RNA-processing complexes."
Krogan N.J., Peng W.-T., Cagney G., Robinson M.D., Haw R., Zhong G., Guo X., Zhang X., Canadien V., Richards D.P., Beattie B.K., Lalev A., Zhang W., Davierwala A.P., Mnaimneh S., Starostine A., Tikuisis A.P., Grigull J. expand/collapse author list , Datta N., Bray J.E., Hughes T.R., Emili A., Greenblatt J.F.
Mol. Cell 13:225-239(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RSC COMPLEX.
[12]"The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion."
Baetz K.K., Krogan N.J., Emili A., Greenblatt J., Hieter P.
Mol. Cell. Biol. 24:1232-1244(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE RSC COMPLEX.
[13]"Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast."
Graumann J., Dunipace L.A., Seol J.H., McDonald W.H., Yates J.R. III, Wold B.J., Deshaies R.J.
Mol. Cell. Proteomics 3:226-237(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE SWI/SNF COMPLEX.
[14]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[15]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84098 Genomic DNA. Translation: CAA58897.1. Different initiation.
Z73060 Genomic DNA. Translation: CAA97305.1. Different initiation.
AY558382 Genomic DNA. Translation: AAS56708.1. Different initiation.
BK006941 Genomic DNA. Translation: DAA08363.1.
PIRS64608.
RefSeqNP_011791.4. NM_001181404.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4I6MX-ray2.80D1-157[»]
ProteinModelPortalP53330.
SMRP53330. Positions 1-90.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33525. 74 interactions.
DIPDIP-3864N.
IntActP53330. 55 interactions.
MINTMINT-522104.
STRING4932.YGR275W.

Proteomic databases

MaxQBP53330.
PaxDbP53330.
PeptideAtlasP53330.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR275W; YGR275W; YGR275W.
GeneID853192.
KEGGsce:YGR275W.

Organism-specific databases

CYGDYGR275w.
SGDS000003507. RTT102.

Phylogenomic databases

eggNOGNOG76281.
OMAHEEISAT.
OrthoDBEOG7RZ63Q.

Enzyme and pathway databases

BioCycYEAST:G3O-30940-MONOMER.

Gene expression databases

GenevestigatorP53330.

Family and domain databases

InterProIPR018304. Tscrpt_reg_Rtt102p.
[Graphical view]
PfamPF09510. Rtt102p. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973347.

Entry information

Entry nameRT102_YEAST
AccessionPrimary (citable) accession number: P53330
Secondary accession number(s): D6VV52
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 13, 2005
Last modified: June 11, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

PDB cross-references

Index of Protein Data Bank (PDB) cross-references