P53330 (RT102_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulator of Ty1 transposition protein 102

Gene names

Name:	RTT102
Ordered Locus Names:	YGR275W
ORF Names:	G9378

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable component of the chromatin structure-remodeling complex (RSC) which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Probable component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.
Subunit structure	Interacts with STH1 and SWI3. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Probable additional component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29. Ref.10 Ref.11 Ref.12 Ref.13
Subcellular location	Nucleus Ref.7.
Miscellaneous	Present with 1550 molecules/cell in log phase SD medium.
Sequence caution	The sequence AAS56708.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA58897.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA97305.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin remodeling Inferred by curator Ref.12. Source: SGD chromosome segregation Inferred from mutant phenotype Ref.12. Source: SGD nucleosome disassembly Inferred from direct assay PubMed 16492771. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription elongation from RNA polymerase II promoter Inferred from direct assay PubMed 17081996. Source: SGD
Cellular_component	RSC complex Inferred from direct assay Ref.12. Source: SGD SWI/SNF complex Inferred from direct assay Ref.10PubMed 18644858. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
STH1	P32597	5	EBI-23637,EBI-18410
SWI3	P32591	2	EBI-23637,EBI-18622

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 157

157

Regulator of Ty1 transposition protein 102

PRO_0000097501

Amino acid modifications

Modified residue

122

1

Phosphoserine Ref.14

Secondary structure

1

.

.

.

.

.

.

.

.

.

157

Helix Strand Turn

Details...

Helix

3 – 9

7

Beta strand

25 – 30

6

Beta strand

60 – 65

6

Beta strand

86 – 88

3

Sequences

	Sequence		Length	Mass (Da)
	P53330 [UniParc]. Last modified September 13, 2005. Version 2. Checksum: D71CA1234D0D0176	FASTA	157	17,795
	10 20 30 40 50 60 MDPQTLITKA NKVSYYGNPT SKESWRYDWY QPSKVSSNVQ QPQQQLGDME NNLEKYPFRY 70 80 90 100 110 120 KTWLRNQEDE KNLQRESCED ILDLKEFDRR ILKKSLMTSH TKGDTSKATG APSANQGDEA 130 140 150 LSVDDIRGAV GNSEAIPGLS AGVNNDNTKE SKDVKMN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145." Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L. Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance." Scholes D.T., Banerjee M., Bowen B., Curcio M.J. Genetics 159:1449-1465(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Finding functional features in Saccharomyces genomes by phylogenetic footprinting." Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M. Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[10]	"Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits." Lee K.K., Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L. Biochem. Soc. Trans. 32:899-903(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH STH1 AND SWI3, IDENTIFICATION IN THE SWI/SNF COMPLEX.
[11]	"High-definition macromolecular composition of yeast RNA-processing complexes." Krogan N.J., Peng W.-T., Cagney G., Robinson M.D., Haw R., Zhong G., Guo X., Zhang X., Canadien V., Richards D.P., Beattie B.K., Lalev A., Zhang W., Davierwala A.P., Mnaimneh S., Starostine A., Tikuisis A.P., Grigull J. , Datta N., Bray J.E., Hughes T.R., Emili A., Greenblatt J.F. Mol. Cell 13:225-239(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RSC COMPLEX.
[12]	"The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion." Baetz K.K., Krogan N.J., Emili A., Greenblatt J., Hieter P. Mol. Cell. Biol. 24:1232-1244(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE RSC COMPLEX.
[13]	"Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast." Graumann J., Dunipace L.A., Seol J.H., McDonald W.H., Yates J.R. III, Wold B.J., Deshaies R.J. Mol. Cell. Proteomics 3:226-237(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SWI/SNF COMPLEX.
[14]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, MASS SPECTROMETRY. Strain: ADR376.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X84098 Genomic DNA. Translation: CAA58897.1. Different initiation.
Z73060 Genomic DNA. Translation: CAA97305.1. Different initiation.
AY558382 Genomic DNA. Translation: AAS56708.1. Different initiation.
BK006941 Genomic DNA. Translation: DAA08363.1.

PIR

S64608.

RefSeq

NP_011791.4. NM_001181404.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4I6M	X-ray	2.80	D	1-157	[»]

ProteinModelPortal

P53330.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3864N.

IntAct

P53330. 54 interactions.

MINT

MINT-522104.

STRING

4932.YGR275W.

Proteomic databases

PaxDb

P53330.

PeptideAtlas

P53330.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGR275W; YGR275W; YGR275W.

GeneID

853192.

KEGG

sce:YGR275W.
sce:YGR281W.

Organism-specific databases

CYGD

YGR275w.

SGD

S000003507. RTT102.

Phylogenomic databases

eggNOG

NOG76281.

OrthoDB

EOG4DBXQK.

Gene expression databases

Genevestigator

P53330.

GermOnline

YGR275W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR018304. Tscrpt_reg_Rtt102p.
[Graphical view]

Pfam

PF09510. Rtt102p. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

973347.

Entry information

Entry name

RT102_YEAST

Accession

Primary (citable) accession number: P53330
Secondary accession number(s): D6VV52

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 13, 2005
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program