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P53330

- RT102_YEAST

UniProt

P53330 - RT102_YEAST

Protein

Regulator of Ty1 transposition protein 102

Gene

RTT102

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (13 Sep 2005)
      Previous versions | rss
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    Functioni

    Probable component of the chromatin structure-remodeling complex (RSC) which is involved in transcription regulation and nucleosome positioning. RSC is responsible for the transfer of a histone octamer from a nucleosome core particle to naked DNA. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. Remodeling reaction also involves DNA translocation, DNA twist and conformational change. As a reconfigurer of centromeric and flanking nucleosomes, RSC complex is required both for proper kinetochore function in chromosome segregation and, via a PKC1-dependent signaling pathway, for organization of the cellular cytoskeleton. Probable component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. chromatin remodeling Source: SGD
    3. chromosome segregation Source: SGD
    4. nucleosome disassembly Source: SGD
    5. regulation of transcription, DNA-templated Source: UniProtKB-KW
    6. transcription elongation from RNA polymerase II promoter Source: SGD

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30940-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulator of Ty1 transposition protein 102
    Gene namesi
    Name:RTT102
    Ordered Locus Names:YGR275W
    ORF Names:G9378
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR275w.
    SGDiS000003507. RTT102.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. RSC complex Source: SGD
    2. SWI/SNF complex Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Regulator of Ty1 transposition protein 102PRO_0000097501Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei77 – 771Phosphoserine1 Publication
    Modified residuei122 – 1221Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP53330.
    PaxDbiP53330.
    PeptideAtlasiP53330.

    Expressioni

    Gene expression databases

    GenevestigatoriP53330.

    Interactioni

    Subunit structurei

    Interacts with STH1 and SWI3. Component of the two forms of the RSC complex composed of at least either RSC1 or RSC2, and ARP7, ARP9, LDB7, NPL6, RSC3, RSC30, RSC4, RSC58, RSC6, RSC8, RSC9, SFH1, STH1, HTL1 and probably RTT102. The complexes interact with histone and histone variant components of centromeric chromatin. Probable additional component of the SWI/SNF global transcription activator complex. The 1.14 MDa SWI/SNF complex is composed of 11 different subunits: one copy each of SWI1, SNF2/SWI2, SNF5, SNF12/SWP73, ARP7/SWP61, ARP9/SWP59; two copies each of SWI3, SNF6, SNF11, SWP82; and three copies of TAF14/SWP29.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    STH1P325975EBI-23637,EBI-18410
    SWI3P325912EBI-23637,EBI-18622

    Protein-protein interaction databases

    BioGridi33525. 74 interactions.
    DIPiDIP-3864N.
    IntActiP53330. 55 interactions.
    MINTiMINT-522104.
    STRINGi4932.YGR275W.

    Structurei

    Secondary structure

    1
    157
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 97
    Beta strandi25 – 306
    Beta strandi60 – 656
    Beta strandi86 – 883

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4I6MX-ray2.80D1-157[»]
    ProteinModelPortaliP53330.
    SMRiP53330. Positions 1-90.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG76281.
    OMAiHEEISAT.
    OrthoDBiEOG7RZ63Q.

    Family and domain databases

    InterProiIPR018304. Tscrpt_reg_Rtt102p.
    [Graphical view]
    PfamiPF09510. Rtt102p. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53330-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDPQTLITKA NKVSYYGNPT SKESWRYDWY QPSKVSSNVQ QPQQQLGDME    50
    NNLEKYPFRY KTWLRNQEDE KNLQRESCED ILDLKEFDRR ILKKSLMTSH 100
    TKGDTSKATG APSANQGDEA LSVDDIRGAV GNSEAIPGLS AGVNNDNTKE 150
    SKDVKMN 157
    Length:157
    Mass (Da):17,795
    Last modified:September 13, 2005 - v2
    Checksum:iD71CA1234D0D0176
    GO

    Sequence cautioni

    The sequence AAS56708.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA58897.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA97305.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84098 Genomic DNA. Translation: CAA58897.1. Different initiation.
    Z73060 Genomic DNA. Translation: CAA97305.1. Different initiation.
    AY558382 Genomic DNA. Translation: AAS56708.1. Different initiation.
    BK006941 Genomic DNA. Translation: DAA08363.1.
    PIRiS64608.
    RefSeqiNP_011791.4. NM_001181404.3.

    Genome annotation databases

    EnsemblFungiiYGR275W; YGR275W; YGR275W.
    GeneIDi853192.
    KEGGisce:YGR275W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84098 Genomic DNA. Translation: CAA58897.1 . Different initiation.
    Z73060 Genomic DNA. Translation: CAA97305.1 . Different initiation.
    AY558382 Genomic DNA. Translation: AAS56708.1 . Different initiation.
    BK006941 Genomic DNA. Translation: DAA08363.1 .
    PIRi S64608.
    RefSeqi NP_011791.4. NM_001181404.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4I6M X-ray 2.80 D 1-157 [» ]
    ProteinModelPortali P53330.
    SMRi P53330. Positions 1-90.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33525. 74 interactions.
    DIPi DIP-3864N.
    IntActi P53330. 55 interactions.
    MINTi MINT-522104.
    STRINGi 4932.YGR275W.

    Proteomic databases

    MaxQBi P53330.
    PaxDbi P53330.
    PeptideAtlasi P53330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR275W ; YGR275W ; YGR275W .
    GeneIDi 853192.
    KEGGi sce:YGR275W.

    Organism-specific databases

    CYGDi YGR275w.
    SGDi S000003507. RTT102.

    Phylogenomic databases

    eggNOGi NOG76281.
    OMAi HEEISAT.
    OrthoDBi EOG7RZ63Q.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30940-MONOMER.

    Miscellaneous databases

    NextBioi 973347.

    Gene expression databases

    Genevestigatori P53330.

    Family and domain databases

    InterProi IPR018304. Tscrpt_reg_Rtt102p.
    [Graphical view ]
    Pfami PF09510. Rtt102p. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145."
      Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.
      Yeast 13:365-368(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Multiple regulators of Ty1 transposition in Saccharomyces cerevisiae have conserved roles in genome maintenance."
      Scholes D.T., Banerjee M., Bowen B., Curcio M.J.
      Genetics 159:1449-1465(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    6. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Finding functional features in Saccharomyces genomes by phylogenetic footprinting."
      Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J., Waterston R., Cohen B.A., Johnston M.
      Science 301:71-76(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    10. "Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits."
      Lee K.K., Prochasson P., Florens L., Swanson S.K., Washburn M.P., Workman J.L.
      Biochem. Soc. Trans. 32:899-903(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH STH1 AND SWI3, IDENTIFICATION IN THE SWI/SNF COMPLEX.
    11. Cited for: IDENTIFICATION IN THE RSC COMPLEX.
    12. "The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion."
      Baetz K.K., Krogan N.J., Emili A., Greenblatt J., Hieter P.
      Mol. Cell. Biol. 24:1232-1244(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE RSC COMPLEX.
    13. "Applicability of tandem affinity purification MudPIT to pathway proteomics in yeast."
      Graumann J., Dunipace L.A., Seol J.H., McDonald W.H., Yates J.R. III, Wold B.J., Deshaies R.J.
      Mol. Cell. Proteomics 3:226-237(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SWI/SNF COMPLEX.
    14. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-122, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRT102_YEAST
    AccessioniPrimary (citable) accession number: P53330
    Secondary accession number(s): D6VV52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: September 13, 2005
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1550 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3