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Protein

Antiviral helicase SLH1

Gene

SLH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Together with SKI2, represses the translation of non-poly(A) mRNAs. May block translation by inhibiting translation initiation factor 5B (FUN12) action on mRNAs lacking a 3' poly(A) structure. Involved in antiviral defense, preventing L-A dsRNA virus propagation by specifically blocking translation of viral mRNAs.2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi310 – 3178ATPPROSITE-ProRule annotation
Nucleotide bindingi1162 – 11698ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro
  • RNA helicase activity Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • defense response to virus Source: UniProtKB-KW
  • regulation of translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Antiviral defense, Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30937-MONOMER.
ReactomeiR-SCE-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral helicase SLH1 (EC:3.6.4.13)
Alternative name(s):
SKI2-like helicase 1
Gene namesi
Name:SLH1
Ordered Locus Names:YGR271W
ORF Names:G9365
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR271W.
SGDiS000003503. SLH1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19671967Antiviral helicase SLH1PRO_0000102085Add
BLAST

Proteomic databases

MaxQBiP53327.
PeptideAtlasiP53327.

PTM databases

iPTMnetiP53327.

Interactioni

Protein-protein interaction databases

BioGridi33520. 43 interactions.
DIPiDIP-5563N.
IntActiP53327. 6 interactions.
MINTiMINT-516762.

Structurei

3D structure databases

ProteinModelPortaliP53327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini297 – 485189Helicase ATP-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini516 – 735220Helicase C-terminal 1PROSITE-ProRule annotationAdd
BLAST
Domaini795 – 1100306SEC63 1Add
BLAST
Domaini1149 – 1324176Helicase ATP-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini1355 – 1550196Helicase C-terminal 2PROSITE-ProRule annotationAdd
BLAST
Domaini1626 – 1776151SEC63 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi427 – 4304DEVH box
Motifi1266 – 12694DEAH box

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated
Contains 2 helicase ATP-binding domains.PROSITE-ProRule annotation
Contains 2 helicase C-terminal domains.PROSITE-ProRule annotation
Contains 2 SEC63 domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00640000091272.
HOGENOMiHOG000157749.
InParanoidiP53327.
KOiK18664.
OMAiGKIMSYY.
OrthoDBiEOG7XDBQ3.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTEYSADSS KSFMIAMQSM IDTSQTFNLD RSKISLPDFD DELKKVQKDE
60 70 80 90 100
QNQRTELTVL SQDRNDWDDI FEEFKDISFA QLQSIIDSYK TKNAVAVYKK
110 120 130 140 150
IGKLINEAET TLSSNVLLET VLQMVYKHQK QELEKELLDF LGTGNIDLVS
160 170 180 190 200
LLLQHRRMIV ATPIETTILL IKNAVNSTPE FLTQQDIRNQ VLKSAEDAKN
210 220 230 240 250
RKLNPATKII KYPHVFRKYE AGSTTAMAFA GQKFTLPVGT TRMSYNTHEE
260 270 280 290 300
IIIPAADQAS NKNYLYTKLL KISDLDHFCK TVFPYETLNQ IQSLVYPVAY
310 320 330 340 350
KTNENMLICA PTGAGKTDIA LLTIINTIKQ FSVVNGENEI DIQYDDFKVI
360 370 380 390 400
YVAPLKALAA EIVDKFSKKL APFNIQVREL TGDMQLTKAE ILATQVIVTT
410 420 430 440 450
PEKWDVVTRK ANGDNDLVSK VKLLIIDEVH LLHEDRGSVI ETLVARTLRQ
460 470 480 490 500
VESSQSMIRI IGLSATLPNF MDVADFLGVN RQIGMFYFDQ SFRPKPLEQQ
510 520 530 540 550
LLGCRGKAGS RQSKENIDKV AYDKLSEMIQ RGYQVMVFVH SRKETVKSAR
560 570 580 590 600
NFIKLAESNH EVDLFAPDPI EKDKYSRSLV KNRDKDMKEI FQFGFGIHHA
610 620 630 640 650
GMARSDRNLT EKMFKDGAIK VLCCTATLAW GVNLPADCVI IKGTQVYDSK
660 670 680 690 700
KGGFIDLGIS DVIQIFGRGG RPGFGSANGT GILCTSNDRL DHYVSLITQQ
710 720 730 740 750
HPIESRFGSK LVDNLNAEIS LGSVTNVDEA IEWLGYTYMF VRMRKNPFTY
760 770 780 790 800
GIDWEEIAND PQLYERRRKM IVVAARRLHA LQMIVFDEVS MHFIAKDLGR
810 820 830 840 850
VSSDFYLLNE SVEIFNQMCD PRATEADVLS MISMSSEFDG IKFREEESKE
860 870 880 890 900
LKRLSDESVE CQIGSQLDTP QGKANVLLQA YISQTRIFDS ALSSDSNYVA
910 920 930 940 950
QNSVRICRAL FLIGVNRRWG KFSNVMLNIC KSIEKRLWAF DHPLCQFDLP
960 970 980 990 1000
ENIIRRIRDT KPSMEHLLEL EADELGELVH NKKAGSRLYK ILSRFPKINI
1010 1020 1030 1040 1050
EAEIFPITTN VMRIHIALGP DFVWDSRIHG DAQFFWVFVE ESDKSQILHF
1060 1070 1080 1090 1100
EKFILNRRQL NNQHEMDFMI PLSDPLPPQV VVKVVSDTWI GCESTHAISF
1110 1120 1130 1140 1150
QHLIRPFNET LQTKLLKLRP LPTSALQNPL IESIYPFKYF NPMQTMTFYT
1160 1170 1180 1190 1200
LYNTNENAFV GSPTGSGKTI VAELAIWHAF KTFPGKKIVY IAPMKALVRE
1210 1220 1230 1240 1250
RVDDWRKKIT PVTGDKVVEL TGDSLPDPKD VHDATIVITT PEKFDGISRN
1260 1270 1280 1290 1300
WQTRKFVQDV SLIIMDEIHL LASDRGPILE MIVSRMNYIS SQTKQPVRLL
1310 1320 1330 1340 1350
GMSTAVSNAY DMAGWLGVKD HGLYNFPSSV RPVPLKMYID GFPDNLAFCP
1360 1370 1380 1390 1400
LMKTMNKPVF MAIKQHSPDK PALIFVASRR QTRLTALDLI HLCGMEDNPR
1410 1420 1430 1440 1450
RFLNIDDEEE LQYYLSQVTD DTLKLSLQFG IGLHHAGLVQ KDRSISHQLF
1460 1470 1480 1490 1500
QKNKIQILIA TSTLAWGVNL PAHLVIIKGT QFFDAKIEGY RDMDLTDILQ
1510 1520 1530 1540 1550
MMGRAGRPAY DTTGTAIVYT KESKKMFYKH FLNVGFPVES SLHKVLDDHL
1560 1570 1580 1590 1600
GAEITSGSIT NKQEALDFLS WTFLFRRAHH NPTYYGIEDD TSTAGVSEHL
1610 1620 1630 1640 1650
SSLIDSTLEN LRESQCVLLH GDDIVATPFL SISSYYYISH LTIRQLLKQI
1660 1670 1680 1690 1700
HDHATFQEVL RWLSLAVEYN ELPVRGGEII MNEEMSQQSR YSVESTFTDE
1710 1720 1730 1740 1750
FELPMWDPHV KTFLLLQAHL SRVDLPIADY IQDTVSVLDQ SLRILQAYID
1760 1770 1780 1790 1800
VASELGYFHT VLTMIKMMQC IKQGYWYEDD PVSVLPGLQL RRIKDYTFSE
1810 1820 1830 1840 1850
QGFIEMTPQQ KKKKLLTLEE IGRFGYKKLL NVFDQLTFGM TESEDTKKRF
1860 1870 1880 1890 1900
VSVCQRLPVL EGMKFEEQEN NEVLTFYSKH LSSKHNNGFE VYCDKFPKIQ
1910 1920 1930 1940 1950
KELWFLIGHK GDELLMIKRC QPKQMNKEVI IHCDLFIPEE IRGEELQFSL
1960
INDALGLRYD MVHKLIS
Length:1,967
Mass (Da):224,850
Last modified:September 21, 2011 - v2
Checksum:i249DF515AEE511A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271F → L in AAC49699 (PubMed:9133744).Curated
Sequence conflicti51 – 511Q → P in AAC49699 (PubMed:9133744).Curated
Sequence conflicti51 – 511Q → P in CAA97301 (PubMed:9169869).Curated
Sequence conflicti193 – 1931K → E in AAC49699 (PubMed:9133744).Curated
Sequence conflicti193 – 1931K → E in CAA97301 (PubMed:9169869).Curated
Sequence conflicti438 – 4381S → P in AAC49699 (PubMed:9133744).Curated
Sequence conflicti438 – 4381S → P in CAA97301 (PubMed:9169869).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35242 Genomic DNA. Translation: AAC49699.1.
Z73056 Genomic DNA. Translation: CAA97301.1.
BK006941 Genomic DNA. Translation: DAA08359.2.
PIRiS64604.
RefSeqiNP_011787.4. NM_001181400.4.

Genome annotation databases

EnsemblFungiiYGR271W; YGR271W; YGR271W.
GeneIDi853187.
KEGGisce:YGR271W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35242 Genomic DNA. Translation: AAC49699.1.
Z73056 Genomic DNA. Translation: CAA97301.1.
BK006941 Genomic DNA. Translation: DAA08359.2.
PIRiS64604.
RefSeqiNP_011787.4. NM_001181400.4.

3D structure databases

ProteinModelPortaliP53327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33520. 43 interactions.
DIPiDIP-5563N.
IntActiP53327. 6 interactions.
MINTiMINT-516762.

PTM databases

iPTMnetiP53327.

Proteomic databases

MaxQBiP53327.
PeptideAtlasiP53327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR271W; YGR271W; YGR271W.
GeneIDi853187.
KEGGisce:YGR271W.

Organism-specific databases

EuPathDBiFungiDB:YGR271W.
SGDiS000003503. SLH1.

Phylogenomic databases

GeneTreeiENSGT00640000091272.
HOGENOMiHOG000157749.
InParanoidiP53327.
KOiK18664.
OMAiGKIMSYY.
OrthoDBiEOG7XDBQ3.

Enzyme and pathway databases

BioCyciYEAST:G3O-30937-MONOMER.
ReactomeiR-SCE-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi973332.
PROiP53327.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR003593. AAA+_ATPase.
IPR000008. C2_dom.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR004179. Sec63-dom.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00270. DEAD. 2 hits.
PF00271. Helicase_C. 2 hits.
PF02889. Sec63. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
SM00487. DEXDc. 2 hits.
SM00490. HELICc. 2 hits.
SM00973. Sec63. 2 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
SSF52540. SSF52540. 4 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 2 hits.
PS51194. HELICASE_CTER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of gene encoding a putative RNA-helicase, homologous to SKI2, in chromosome VII of Saccharomyces cerevisiae."
    Martegani E., Vanoni M., Mauri I., Rudoni S., Saliola M., Alberghina L.
    Yeast 13:391-397(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 51; 193 AND 438.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION.
  5. "Linking the 3' poly(A) tail to the subunit joining step of translation initiation: relations of Pab1p, eukaryotic translation initiation factor 5b (Fun12p), and Ski2p-Slh1p."
    Searfoss A., Dever T.E., Wickner R.
    Mol. Cell. Biol. 21:4900-4908(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSLH1_YEAST
AccessioniPrimary (citable) accession number: P53327
Secondary accession number(s): D6VV48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: May 11, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 486 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.