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Protein

Antiviral helicase SLH1

Gene

SLH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with SKI2, represses the translation of non-poly(A) mRNAs. May block translation by inhibiting translation initiation factor 5B (FUN12) action on mRNAs lacking a 3' poly(A) structure. Involved in antiviral defense, preventing L-A dsRNA virus propagation by specifically blocking translation of viral mRNAs.2 Publications

Miscellaneous

Present with 486 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi310 – 317ATPPROSITE-ProRule annotation8
Nucleotide bindingi1162 – 1169ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • nucleic acid binding Source: InterPro
  • RNA helicase activity Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • defense response to virus Source: UniProtKB-KW
  • regulation of translation Source: SGD
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processAntiviral defense, Translation regulation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30937-MONOMER
ReactomeiR-SCE-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral helicase SLH1 (EC:3.6.4.13)
Alternative name(s):
SKI2-like helicase 1
Gene namesi
Name:SLH1
Ordered Locus Names:YGR271W
ORF Names:G9365
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR271W
SGDiS000003503 SLH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020851 – 1967Antiviral helicase SLH1Add BLAST1967

Proteomic databases

MaxQBiP53327
PaxDbiP53327
PRIDEiP53327

PTM databases

iPTMnetiP53327

Interactioni

Protein-protein interaction databases

BioGridi33520, 133 interactors
DIPiDIP-5563N
IntActiP53327, 6 interactors
MINTiP53327
STRINGi4932.YGR271W

Structurei

3D structure databases

ProteinModelPortaliP53327
SMRiP53327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini297 – 485Helicase ATP-binding 1PROSITE-ProRule annotationAdd BLAST189
Domaini516 – 735Helicase C-terminal 1PROSITE-ProRule annotationAdd BLAST220
Domaini795 – 1100SEC63 1Add BLAST306
Domaini1149 – 1324Helicase ATP-binding 2PROSITE-ProRule annotationAdd BLAST176
Domaini1355 – 1550Helicase C-terminal 2PROSITE-ProRule annotationAdd BLAST196
Domaini1626 – 1776SEC63 2Add BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi427 – 430DEVH box4
Motifi1266 – 1269DEAH box4

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00640000091272
HOGENOMiHOG000157749
InParanoidiP53327
KOiK18664
OMAiNAKPNYG
OrthoDBiEOG092C01UD

Family and domain databases

CDDicd00079 HELICc, 2 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR035892 C2_domain_sf
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR004179 Sec63-dom
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00270 DEAD, 2 hits
PF00271 Helicase_C, 2 hits
PF02889 Sec63, 2 hits
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM00487 DEXDc, 2 hits
SM00490 HELICc, 2 hits
SM00973 Sec63, 2 hits
SUPFAMiSSF46785 SSF46785, 2 hits
SSF52540 SSF52540, 4 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 2 hits
PS51194 HELICASE_CTER, 2 hits

Sequencei

Sequence statusi: Complete.

P53327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTEYSADSS KSFMIAMQSM IDTSQTFNLD RSKISLPDFD DELKKVQKDE
60 70 80 90 100
QNQRTELTVL SQDRNDWDDI FEEFKDISFA QLQSIIDSYK TKNAVAVYKK
110 120 130 140 150
IGKLINEAET TLSSNVLLET VLQMVYKHQK QELEKELLDF LGTGNIDLVS
160 170 180 190 200
LLLQHRRMIV ATPIETTILL IKNAVNSTPE FLTQQDIRNQ VLKSAEDAKN
210 220 230 240 250
RKLNPATKII KYPHVFRKYE AGSTTAMAFA GQKFTLPVGT TRMSYNTHEE
260 270 280 290 300
IIIPAADQAS NKNYLYTKLL KISDLDHFCK TVFPYETLNQ IQSLVYPVAY
310 320 330 340 350
KTNENMLICA PTGAGKTDIA LLTIINTIKQ FSVVNGENEI DIQYDDFKVI
360 370 380 390 400
YVAPLKALAA EIVDKFSKKL APFNIQVREL TGDMQLTKAE ILATQVIVTT
410 420 430 440 450
PEKWDVVTRK ANGDNDLVSK VKLLIIDEVH LLHEDRGSVI ETLVARTLRQ
460 470 480 490 500
VESSQSMIRI IGLSATLPNF MDVADFLGVN RQIGMFYFDQ SFRPKPLEQQ
510 520 530 540 550
LLGCRGKAGS RQSKENIDKV AYDKLSEMIQ RGYQVMVFVH SRKETVKSAR
560 570 580 590 600
NFIKLAESNH EVDLFAPDPI EKDKYSRSLV KNRDKDMKEI FQFGFGIHHA
610 620 630 640 650
GMARSDRNLT EKMFKDGAIK VLCCTATLAW GVNLPADCVI IKGTQVYDSK
660 670 680 690 700
KGGFIDLGIS DVIQIFGRGG RPGFGSANGT GILCTSNDRL DHYVSLITQQ
710 720 730 740 750
HPIESRFGSK LVDNLNAEIS LGSVTNVDEA IEWLGYTYMF VRMRKNPFTY
760 770 780 790 800
GIDWEEIAND PQLYERRRKM IVVAARRLHA LQMIVFDEVS MHFIAKDLGR
810 820 830 840 850
VSSDFYLLNE SVEIFNQMCD PRATEADVLS MISMSSEFDG IKFREEESKE
860 870 880 890 900
LKRLSDESVE CQIGSQLDTP QGKANVLLQA YISQTRIFDS ALSSDSNYVA
910 920 930 940 950
QNSVRICRAL FLIGVNRRWG KFSNVMLNIC KSIEKRLWAF DHPLCQFDLP
960 970 980 990 1000
ENIIRRIRDT KPSMEHLLEL EADELGELVH NKKAGSRLYK ILSRFPKINI
1010 1020 1030 1040 1050
EAEIFPITTN VMRIHIALGP DFVWDSRIHG DAQFFWVFVE ESDKSQILHF
1060 1070 1080 1090 1100
EKFILNRRQL NNQHEMDFMI PLSDPLPPQV VVKVVSDTWI GCESTHAISF
1110 1120 1130 1140 1150
QHLIRPFNET LQTKLLKLRP LPTSALQNPL IESIYPFKYF NPMQTMTFYT
1160 1170 1180 1190 1200
LYNTNENAFV GSPTGSGKTI VAELAIWHAF KTFPGKKIVY IAPMKALVRE
1210 1220 1230 1240 1250
RVDDWRKKIT PVTGDKVVEL TGDSLPDPKD VHDATIVITT PEKFDGISRN
1260 1270 1280 1290 1300
WQTRKFVQDV SLIIMDEIHL LASDRGPILE MIVSRMNYIS SQTKQPVRLL
1310 1320 1330 1340 1350
GMSTAVSNAY DMAGWLGVKD HGLYNFPSSV RPVPLKMYID GFPDNLAFCP
1360 1370 1380 1390 1400
LMKTMNKPVF MAIKQHSPDK PALIFVASRR QTRLTALDLI HLCGMEDNPR
1410 1420 1430 1440 1450
RFLNIDDEEE LQYYLSQVTD DTLKLSLQFG IGLHHAGLVQ KDRSISHQLF
1460 1470 1480 1490 1500
QKNKIQILIA TSTLAWGVNL PAHLVIIKGT QFFDAKIEGY RDMDLTDILQ
1510 1520 1530 1540 1550
MMGRAGRPAY DTTGTAIVYT KESKKMFYKH FLNVGFPVES SLHKVLDDHL
1560 1570 1580 1590 1600
GAEITSGSIT NKQEALDFLS WTFLFRRAHH NPTYYGIEDD TSTAGVSEHL
1610 1620 1630 1640 1650
SSLIDSTLEN LRESQCVLLH GDDIVATPFL SISSYYYISH LTIRQLLKQI
1660 1670 1680 1690 1700
HDHATFQEVL RWLSLAVEYN ELPVRGGEII MNEEMSQQSR YSVESTFTDE
1710 1720 1730 1740 1750
FELPMWDPHV KTFLLLQAHL SRVDLPIADY IQDTVSVLDQ SLRILQAYID
1760 1770 1780 1790 1800
VASELGYFHT VLTMIKMMQC IKQGYWYEDD PVSVLPGLQL RRIKDYTFSE
1810 1820 1830 1840 1850
QGFIEMTPQQ KKKKLLTLEE IGRFGYKKLL NVFDQLTFGM TESEDTKKRF
1860 1870 1880 1890 1900
VSVCQRLPVL EGMKFEEQEN NEVLTFYSKH LSSKHNNGFE VYCDKFPKIQ
1910 1920 1930 1940 1950
KELWFLIGHK GDELLMIKRC QPKQMNKEVI IHCDLFIPEE IRGEELQFSL
1960
INDALGLRYD MVHKLIS
Length:1,967
Mass (Da):224,850
Last modified:September 21, 2011 - v2
Checksum:i249DF515AEE511A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27F → L in AAC49699 (PubMed:9133744).Curated1
Sequence conflicti51Q → P in AAC49699 (PubMed:9133744).Curated1
Sequence conflicti51Q → P in CAA97301 (PubMed:9169869).Curated1
Sequence conflicti193K → E in AAC49699 (PubMed:9133744).Curated1
Sequence conflicti193K → E in CAA97301 (PubMed:9169869).Curated1
Sequence conflicti438S → P in AAC49699 (PubMed:9133744).Curated1
Sequence conflicti438S → P in CAA97301 (PubMed:9169869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U35242 Genomic DNA Translation: AAC49699.1
Z73056 Genomic DNA Translation: CAA97301.1
BK006941 Genomic DNA Translation: DAA08359.2
PIRiS64604
RefSeqiNP_011787.4, NM_001181400.4

Genome annotation databases

EnsemblFungiiYGR271W; YGR271W; YGR271W
GeneIDi853187
KEGGisce:YGR271W

Similar proteinsi

Entry informationi

Entry nameiSLH1_YEAST
AccessioniPrimary (citable) accession number: P53327
Secondary accession number(s): D6VV48
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: September 21, 2011
Last modified: May 23, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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