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Protein

Steryl acetyl hydrolase 1

Gene

SAY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the deacetylation of acetylated sterols. Involved in the resistance to eugenol and pregnenolone toxicity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei250By similarity1
Active sitei395By similarity1

GO - Molecular functioni

  • steryl deacetylase activity Source: SGD

GO - Biological processi

  • catabolic process Source: GO_Central
  • response to toxic substance Source: SGD
  • sterol deacetylation Source: SGD
  • sterol metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-30932-MONOMER

Protein family/group databases

ESTHERiyeast-SAY1 Steryl_acetyl_hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Steryl acetyl hydrolase 1 (EC:3.1.1.-)
Gene namesi
Name:SAY1
Ordered Locus Names:YGR263C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR263C
SGDiS000003495 SAY1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 45CytoplasmicSequence analysisAdd BLAST44
Transmembranei46 – 66Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini67 – 424LumenalSequence analysisAdd BLAST358

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002028622 – 424Steryl acetyl hydrolase 1Add BLAST423

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Glycosylationi85N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi283N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

MaxQBiP53324
PaxDbiP53324
PRIDEiP53324

PTM databases

iPTMnetiP53324

Interactioni

Protein-protein interaction databases

BioGridi33514, 80 interactors
DIPiDIP-5103N
IntActiP53324, 17 interactors
MINTiP53324
STRINGi4932.YGR263C

Structurei

3D structure databases

ProteinModelPortaliP53324
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi176 – 178Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity3

Sequence similaritiesi

Belongs to the 'GDXG' lipolytic enzyme family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP53324
OMAiICQYVLN
OrthoDBiEOG092C250X

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR019436 Say1-like
PfamiView protein in Pfam
PF10340 Say1_Mug180, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANSGLDSK VEYYRLQENE IISAVSSEDA DQNDAGFRLS TIHLHLFHGL
60 70 80 90 100
KFAALLFTVV PVFIILDSMK IIFQRKRRFC LDHVNRSFLR QSSWILDERI
110 120 130 140 150
CQYVLNPLFV CLYPSTFSSP TYVKCNIPIE DQKSPENNIF QDHQLNAPKI
160 170 180 190 200
VSTKFYQYVM PEGFDPTTDP VLVFYHGGGY ALKLTPTSFS FLNNMRNAFP
210 220 230 240 250
KMAILVPDYT VTATDDQSKK YPLQILQNVA IFDYVVKTMG CKNVVIMGDS
260 270 280 290 300
AGGNAVLNIV LYLRKCHREI YPKKVIAISP WANATFFHEG EKEYMQGTQE
310 320 330 340 350
WDGLCLKSHS MFGRMFVGNN PNVDFTSDPF VNIEKNFETK MWQDILKKCS
360 370 380 390 400
VMITYGSDEL LSFQNKILAK KMSDASEGCN HFTAKNVLVE HQGYHTGPIL
410 420
NYSRNMDRWT NIPSIARILE FMQS
Length:424
Mass (Da):48,541
Last modified:October 1, 1996 - v1
Checksum:iEB0D1D6078CD6516
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07777 Genomic DNA Translation: CAA69085.1
Z73048 Genomic DNA Translation: CAA97292.1
Z73049 Genomic DNA Translation: CAA97295.1
BK006941 Genomic DNA Translation: DAA08353.1
PIRiS64596
RefSeqiNP_011779.3, NM_001181392.3

Genome annotation databases

EnsemblFungiiYGR263C; YGR263C; YGR263C
GeneIDi853179
KEGGisce:YGR263C

Similar proteinsi

Entry informationi

Entry nameiSAY1_YEAST
AccessioniPrimary (citable) accession number: P53324
Secondary accession number(s): D6VV42
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health