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Protein

Steryl acetyl hydrolase 1

Gene

SAY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the deacetylation of acetylated sterols. Involved in the resistance to eugenol and pregnenolone toxicity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei250 – 2501By similarity
Active sitei395 – 3951By similarity

GO - Molecular functioni

  • steryl deacetylase activity Source: SGD

GO - Biological processi

  • response to toxic substance Source: SGD
  • sterol deacetylation Source: SGD
  • sterol metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-30932-MONOMER.

Protein family/group databases

ESTHERiyeast-SAY1. Steryl_acetyl_hydrolase.

Names & Taxonomyi

Protein namesi
Recommended name:
Steryl acetyl hydrolase 1 (EC:3.1.1.-)
Gene namesi
Name:SAY1
Ordered Locus Names:YGR263C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR263C.
SGDiS000003495. SAY1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 4544CytoplasmicSequence analysisAdd
BLAST
Transmembranei46 – 6621Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini67 – 424358LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum lumen Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: SGD
  • lipid particle Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 424423Steryl acetyl hydrolase 1PRO_0000202862Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Glycosylationi85 – 851N-linked (GlcNAc...)Sequence analysis
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

MaxQBiP53324.

Interactioni

Protein-protein interaction databases

BioGridi33514. 19 interactions.
DIPiDIP-5103N.
IntActiP53324. 3 interactions.
MINTiMINT-476303.

Structurei

3D structure databases

ProteinModelPortaliP53324.
SMRiP53324. Positions 172-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi176 – 1783Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion holeBy similarity

Sequence similaritiesi

Belongs to the 'GDXG' lipolytic enzyme family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiP53324.
OMAiICQYVLN.
OrthoDBiEOG092C250X.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR019436. Say1-like.
[Graphical view]
PfamiPF10340. DUF2424. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P53324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAANSGLDSK VEYYRLQENE IISAVSSEDA DQNDAGFRLS TIHLHLFHGL
60 70 80 90 100
KFAALLFTVV PVFIILDSMK IIFQRKRRFC LDHVNRSFLR QSSWILDERI
110 120 130 140 150
CQYVLNPLFV CLYPSTFSSP TYVKCNIPIE DQKSPENNIF QDHQLNAPKI
160 170 180 190 200
VSTKFYQYVM PEGFDPTTDP VLVFYHGGGY ALKLTPTSFS FLNNMRNAFP
210 220 230 240 250
KMAILVPDYT VTATDDQSKK YPLQILQNVA IFDYVVKTMG CKNVVIMGDS
260 270 280 290 300
AGGNAVLNIV LYLRKCHREI YPKKVIAISP WANATFFHEG EKEYMQGTQE
310 320 330 340 350
WDGLCLKSHS MFGRMFVGNN PNVDFTSDPF VNIEKNFETK MWQDILKKCS
360 370 380 390 400
VMITYGSDEL LSFQNKILAK KMSDASEGCN HFTAKNVLVE HQGYHTGPIL
410 420
NYSRNMDRWT NIPSIARILE FMQS
Length:424
Mass (Da):48,541
Last modified:October 1, 1996 - v1
Checksum:iEB0D1D6078CD6516
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07777 Genomic DNA. Translation: CAA69085.1.
Z73048 Genomic DNA. Translation: CAA97292.1.
Z73049 Genomic DNA. Translation: CAA97295.1.
BK006941 Genomic DNA. Translation: DAA08353.1.
PIRiS64596.
RefSeqiNP_011779.3. NM_001181392.3.

Genome annotation databases

EnsemblFungiiYGR263C; YGR263C; YGR263C.
GeneIDi853179.
KEGGisce:YGR263C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07777 Genomic DNA. Translation: CAA69085.1.
Z73048 Genomic DNA. Translation: CAA97292.1.
Z73049 Genomic DNA. Translation: CAA97295.1.
BK006941 Genomic DNA. Translation: DAA08353.1.
PIRiS64596.
RefSeqiNP_011779.3. NM_001181392.3.

3D structure databases

ProteinModelPortaliP53324.
SMRiP53324. Positions 172-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33514. 19 interactions.
DIPiDIP-5103N.
IntActiP53324. 3 interactions.
MINTiMINT-476303.

Protein family/group databases

ESTHERiyeast-SAY1. Steryl_acetyl_hydrolase.

Proteomic databases

MaxQBiP53324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR263C; YGR263C; YGR263C.
GeneIDi853179.
KEGGisce:YGR263C.

Organism-specific databases

EuPathDBiFungiDB:YGR263C.
SGDiS000003495. SAY1.

Phylogenomic databases

InParanoidiP53324.
OMAiICQYVLN.
OrthoDBiEOG092C250X.

Enzyme and pathway databases

BioCyciYEAST:G3O-30932-MONOMER.

Miscellaneous databases

PROiP53324.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR019436. Say1-like.
[Graphical view]
PfamiPF10340. DUF2424. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAY1_YEAST
AccessioniPrimary (citable) accession number: P53324
Secondary accession number(s): D6VV42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.