P53323 (BUD32_YEAST) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 114.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Serine/threonine-protein kinase BUD32 EC=2.7.11.1 Alternative name(s): Bud site selection protein BUD32 Low-dye-binding protein 14 piD261 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | ||||||
| Taxonomic identifier | 559292 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 261 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection. Ref.9 Ref.10 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1. |
| Subcellular location | |
| Miscellaneous | Present with 3020 molecules/cell in log phase SD medium. Ref.8 |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| CGI121 | Q03705 | 8 | EBI-3809,EBI-912262 | |
| GON7 | P46984 | 7 | EBI-3809,EBI-26178 | |
| GRX3 | Q03835 | 3 | EBI-3809,EBI-22178 | |
| GRX4 | P32642 | 9 | EBI-3809,EBI-22211 | |
| KAE1 | P36132 | 12 | EBI-3809,EBI-26411 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 261 | 261 | Serine/threonine-protein kinase BUD32 | PRO_0000088189 | |||||
Regions | |||||||||
| Domain | 16 – 261 | 246 | Protein kinase | ||||||
| Nucleotide binding | 22 – 30 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 161 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 43 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 187 | 1 | Phosphoserine; by autocatalysis Ref.6 | ||||||
| Modified residue | 189 | 1 | Phosphoserine; by autocatalysis Ref.6 | ||||||
Experimental info | |||||||||
| Mutagenesis | 187 | 1 | S → A: Reduced kinase activity. Ref.6 | ||||||
| Mutagenesis | 189 | 1 | S → A: Reduced kinase activity. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes." Clemente M.L., Sartori G., Cardazzo B., Carignani G. Yeast 13:287-290(1997) [PubMed: 9090059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c. |
| [4] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [5] | "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: INVOLVEMENT IN BUDDING. |
| [6] | "Structure-function analysis of yeast piD261/Bud32, an atypical protein kinase essential for normal cell life." Facchin S., Lopreiato R., Stocchetto S., Arrigoni G., Cesaro L., Marin O., Carignani G., Pinna L.A. Biochem. J. 364:457-463(2002) [PubMed: 12023889] [Abstract] Cited for: PHOSPHORYLATION AT SER-187 AND SER-189, MUTAGENESIS OF SER-187 AND SER-189. |
| [7] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [8] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [9] | "Yeast homolog of a cancer-testis antigen defines a new transcription complex." Kisseleva-Romanova E., Lopreiato R., Baudin-Baillieu A., Rousselle J.-C., Ilan L., Hofmann K., Namane A., Mann C., Libri D. EMBO J. 25:3576-3585(2006) [PubMed: 16874308] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE EKC/KEOPS COMPLEX, FUNCTION OF THE EKC/KEOPS COMPLEX. |
| [10] | "A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator." Downey M., Houlsworth R., Maringele L., Rollie A., Brehme M., Galicia S., Guillard S., Partington M., Zubko M.K., Krogan N.J., Emili A., Greenblatt J.F., Harrington L., Lydall D., Durocher D. Cell 124:1155-1168(2006) [PubMed: 16564010] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EKC/KEOPS COMPLEX. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y07777 Genomic DNA. Translation: CAA69084.1. Z73047 Genomic DNA. Translation: CAA97291.1. AY558547 Genomic DNA. Translation: AAS56873.1. BK006941 Genomic DNA. Translation: DAA08352.1. |
| PIR | S64595. |
| RefSeq | NP_011778.1. NM_001181391.1. |
3D structure databases | |
| ProteinModelPortal | P53323. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-5008N. |
| IntAct | P53323. 43 interactions. |
| MINT | MINT-571543. |
| STRING | P53323. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | YGR262C; YGR262C; YGR262C. |
| GeneID | 853178. |
| KEGG | sce:YGR262C. |
| NMPDR | fig|4932.3.peg.2907. |
Organism-specific databases | |
| CYGD | YGR262c. |
| SGD | S000003494. BUD32. |
Phylogenomic databases | |
| eggNOG | fuNOG08130. |
| GeneTree | EFGT00050000005785. |
| HOGENOM | HBG574115. |
| OMA | MLLKPPL. |
| OrthoDB | EOG4R7ZKZ. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 984. |
Gene expression databases | |
| ArrayExpress | P53323. |
| Genevestigator | P53323. |
| GermOnline | YGR262C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR022495. Kinase-assoc_endopept-1. IPR011009. Kinase-like_dom. IPR010440. LipoPS_kinase. IPR000719. Prot_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view] |
| KO | K08851. |
| Pfam | PF06293. Kdo. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| TIGRFAMs | TIGR03724. Arch_bud32. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 973311. |
Entry information
| Entry name | BUD32_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53323 Secondary accession number(s): D6VV41 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with