Reviewed,
UniProtKB/Swiss-Prot P53323 (BUD32_YEAST)
Last modified
June 16, 2009.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Serine/threonine-protein kinase BUD32 EC=2.7.11.1 Alternative name(s): Bud site selection protein BUD32 piD261 Low-dye-binding protein 14 | ||||||
| Gene names |
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| Organism | Saccharomyces cerevisiae (Baker's yeast) [Complete proteome] | ||||||
| Taxonomic identifier | 4932 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces |
Protein attributes
| Sequence length | 261 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection. Ref.8 Ref.9 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subunit structure | Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1. |
| Subcellular location | |
| Miscellaneous | Present with 3020 molecules/cell in log phase SD medium. Ref.7 |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| P38800 | 1 | EBI-3809,EBI-24597 | ||
| CGI121 | Q03705 | 1 | EBI-3809,EBI-912262 | |
| GON7 | P46984 | 1 | EBI-3809,EBI-26178 | |
| GRX4 | P32642 | 5 | EBI-3809,EBI-22211 | |
| KAE1 | P36132 | 2 | EBI-3809,EBI-26411 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 261 | 261 | Serine/threonine-protein kinase BUD32 | PRO_0000088189 | |||||
Regions | |||||||||
| Domain | 16 – 261 | 246 | Protein kinase | ||||||
| Nucleotide binding | 22 – 30 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 161 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 43 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 187 | 1 | Phosphoserine; by autocatalysis Ref.5 | ||||||
| Modified residue | 189 | 1 | Phosphoserine; by autocatalysis Ref.5 | ||||||
Experimental info | |||||||||
| Mutagenesis | 187 | 1 | S → A: Reduced kinase activity. Ref.5 | ||||||
| Mutagenesis | 189 | 1 | S → A: Reduced kinase activity. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes." Clemente M.L., Sartori G., Cardazzo B., Carignani G. Yeast 13:287-290(1997) [PubMed: 9090059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [2] | "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K.Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679. |
| [3] | "Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J.Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c. |
| [4] | "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: INVOLVEMENT IN BUDDING. |
| [5] | "Structure-function analysis of yeast piD261/Bud32, an atypical protein kinase essential for normal cell life." Facchin S., Lopreiato R., Stocchetto S., Arrigoni G., Cesaro L., Marin O., Carignani G., Pinna L.A. Biochem. J. 364:457-463(2002) [PubMed: 12023889] [Abstract] Cited for: PHOSPHORYLATION AT SER-187 AND SER-189, MUTAGENESIS OF SER-187 AND SER-189. |
| [6] | "Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| [7] | "Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. |
| [8] | "Yeast homolog of a cancer-testis antigen defines a new transcription complex." Kisseleva-Romanova E., Lopreiato R., Baudin-Baillieu A., Rousselle J.-C., Ilan L., Hofmann K., Namane A., Mann C., Libri D. EMBO J. 25:3576-3585(2006) [PubMed: 16874308] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE EKC/KEOPS COMPLEX, FUNCTION OF THE EKC/KEOPS COMPLEX. |
| [9] | "A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator." Downey M., Houlsworth R., Maringele L., Rollie A., Brehme M., Galicia S., Guillard S., Partington M., Zubko M.K., Krogan N.J., Emili A., Greenblatt J.F., Harrington L., Lydall D., Durocher D. Cell 124:1155-1168(2006) [PubMed: 16564010] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EKC/KEOPS COMPLEX. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| Y07777 Genomic DNA. Translation: CAA69084.1. Z73047 Genomic DNA. Translation: CAA97291.1. AY558547 Genomic DNA. Translation: AAS56873.1. | |
| PIR | S64595. |
| RefSeq | NP_011778.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:5008N. |
| IntAct | P53323. 41 interactions. |
Proteomic databases | |
| PRIDE | P53323. |
Genome annotation databases | |
| Ensembl | YGR262C. Saccharomyces cerevisiae. [Contig view] |
| GeneID | 853178. |
| GenomeReviews | Gene locus YGR262C in contig Y13135_GR. |
| KEGG | sce:YGR262C. |
| NMPDR | fig|4932.3.peg.2907. |
Organism-specific databases | |
| CYGD | YGR262c. |
| SGD | S000003494. BUD32. |
| Yeast-GFP | Search... |
Phylogenomic databases | |
| HOGENOM | P53323. |
| OMA | P53323. VVYFVDY. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 250. |
Gene expression databases | |
| GermOnline | YGR262C. Saccharomyces cerevisiae. |
Family and domain databases | |
| InterPro | IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR008266. Tyr_pkinase_AS. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 973311. |
Entry information
| Entry name | BUD32_YEAST | ||||||||
| Accession | Primary (citable) accession number: P53323 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |
| Yeast Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD |
| Yeast chromosome VII Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names |

Clusters with


