P53323 (BUD32_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase BUD32
EC=2.7.11.1

Alternative name(s):
Bud site selection protein BUD32
Low-dye-binding protein 14
piD261

Gene names

Name:	BUD32
Synonyms:	LDB14
Ordered Locus Names:	YGR262C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection. Ref.9 Ref.10
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1.
Subcellular location	Cytoplasm. Nucleus. Chromosome › telomere Ref.7.
Miscellaneous	Present with 3020 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular bud site selection Inferred from mutant phenotype Ref.5. Source: SGD lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro positive regulation of transcription from RNA polymerase II promoter Inferred from physical interaction Ref.9. Source: SGD telomere maintenance Inferred from mutant phenotype Ref.10. Source: SGD threonylcarbamoyladenosine metabolic process Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	EKC/KEOPS complex Inferred from direct assay Ref.10Ref.9. Source: SGD chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay Ref.7. Source: SGD membrane Inferred from electronic annotation. Source: InterPro nucleus Inferred from direct assay Ref.7. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Inferred from direct assay. Source: SGD protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CGI121	Q03705	8	EBI-3809,EBI-912262
GON7	P46984	7	EBI-3809,EBI-26178
GRX3	Q03835	3	EBI-3809,EBI-22178
GRX4	P32642	9	EBI-3809,EBI-22211
KAE1	P36132	12	EBI-3809,EBI-26411

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Serine/threonine-protein kinase BUD32		PRO_0000088189
Regions
	Domain	16 – 261	246	Protein kinase
	Nucleotide binding	22 – 30	9	ATP By similarity
Sites
	Active site	161	1	Proton acceptor By similarity
	Binding site	43	1	ATP By similarity
Amino acid modifications
	Modified residue	187	1	Phosphoserine; by autocatalysis Ref.6
	Modified residue	189	1	Phosphoserine; by autocatalysis Ref.6
Experimental info
	Mutagenesis	187	1	S → A: Reduced kinase activity. Ref.6
	Mutagenesis	189	1	S → A: Reduced kinase activity. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P53323 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D12E97BAE21B3385	FASTA	261	29,936
	10 20 30 40 50 60 MTQEFIDKVS SYLTPDVDIA PISQGAEAIV FTTTTHPYLP RAKDSHQKYI IKYRPPKRYR 70 80 90 100 110 120 HPQIDQALTK HRTLNESRLL AKLYLIPGLC VPQLIACDPY NGFIWLEFLG EDLPGGHGFS 130 140 150 160 170 180 NLKNFLWMHD QDPYSDLVAT TLRKVGRQIG LLHWNDYCHG DLTSSNIVLV RDGARWTPHL 190 200 210 220 230 240 IDFGLGSVSN LVEDKGVDLY VLERAILSTH SKHAEKYNAW IMEGFEEVYR EQGAKGAKKL 250 260 KEVTKRFEEV RLRGRKRSML G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes." Clemente M.L., Sartori G., Cardazzo B., Carignani G. Yeast 13:287-290(1997) [PubMed: 9090059] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: INVOLVEMENT IN BUDDING.
[6]	"Structure-function analysis of yeast piD261/Bud32, an atypical protein kinase essential for normal cell life." Facchin S., Lopreiato R., Stocchetto S., Arrigoni G., Cesaro L., Marin O., Carignani G., Pinna L.A. Biochem. J. 364:457-463(2002) [PubMed: 12023889] [Abstract] Cited for: PHOSPHORYLATION AT SER-187 AND SER-189, MUTAGENESIS OF SER-187 AND SER-189.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Yeast homolog of a cancer-testis antigen defines a new transcription complex." Kisseleva-Romanova E., Lopreiato R., Baudin-Baillieu A., Rousselle J.-C., Ilan L., Hofmann K., Namane A., Mann C., Libri D. EMBO J. 25:3576-3585(2006) [PubMed: 16874308] [Abstract] Cited for: MASS SPECTROMETRY, IDENTIFICATION IN THE EKC/KEOPS COMPLEX, FUNCTION OF THE EKC/KEOPS COMPLEX.
[10]	"A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator." Downey M., Houlsworth R., Maringele L., Rollie A., Brehme M., Galicia S., Guillard S., Partington M., Zubko M.K., Krogan N.J., Emili A., Greenblatt J.F., Harrington L., Lydall D., Durocher D. Cell 124:1155-1168(2006) [PubMed: 16564010] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE EKC/KEOPS COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y07777 Genomic DNA. Translation: CAA69084.1. Z73047 Genomic DNA. Translation: CAA97291.1. AY558547 Genomic DNA. Translation: AAS56873.1. BK006941 Genomic DNA. Translation: DAA08352.1.
PIR	S64595.
RefSeq	NP_011778.1. NM_001181391.1.
3D structure databases
ProteinModelPortal	P53323.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5008N.
IntAct	P53323. 43 interactions.
MINT	MINT-571543.
STRING	P53323.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR262C; YGR262C; YGR262C.
GeneID	853178.
KEGG	sce:YGR262C.
NMPDR	fig|4932.3.peg.2907.
Organism-specific databases
CYGD	YGR262c.
SGD	S000003494. BUD32.
Phylogenomic databases
eggNOG	fuNOG08130.
GeneTree	EFGT00050000005785.
HOGENOM	HBG574115.
OMA	MLLKPPL.
OrthoDB	EOG4R7ZKZ.
Enzyme and pathway databases
BRENDA	2.7.11.1. 984.
Gene expression databases
ArrayExpress	P53323.
Genevestigator	P53323.
GermOnline	YGR262C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022495. Kinase-assoc_endopept-1. IPR011009. Kinase-like_dom. IPR010440. LipoPS_kinase. IPR000719. Prot_kinase_cat_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
KO	K08851.
Pfam	PF06293. Kdo. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. Arch_bud32. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973311.

Entry information

Entry name

BUD32_YEAST

Accession

Primary (citable) accession number: P53323
Secondary accession number(s): D6VV41

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program