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P53322 (TNA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High-affinity nicotinic acid transporter
Alternative name(s):
Nicotinic acid permease
Gene names
Name:TNA1
Ordered Locus Names:YGR260W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the uptake of nicotinic acid.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the major facilitator superfamily. Allantoate permease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534High-affinity nicotinic acid transporter
PRO_0000121369

Regions

Topological domain1 – 130130Extracellular Potential
Transmembrane131 – 15121Helical; Potential
Topological domain1521Cytoplasmic Potential
Transmembrane153 – 17321Helical; Potential
Topological domain174 – 18714Extracellular Potential
Transmembrane188 – 20821Helical; Potential
Topological domain209 – 2179Cytoplasmic Potential
Transmembrane218 – 23821Helical; Potential
Topological domain239 – 25012Extracellular Potential
Transmembrane251 – 27121Helical; Potential
Topological domain272 – 32352Cytoplasmic Potential
Transmembrane324 – 34421Helical; Potential
Topological domain345 – 35511Extracellular Potential
Transmembrane356 – 37621Helical; Potential
Topological domain377 – 3848Cytoplasmic Potential
Transmembrane385 – 40521Helical; Potential
Topological domain406 – 4105Extracellular Potential
Transmembrane411 – 43121Helical; Potential
Topological domain432 – 44413Cytoplasmic Potential
Transmembrane445 – 46521Helical; Potential
Topological domain466 – 4749Extracellular Potential
Transmembrane475 – 49521Helical; Potential
Topological domain496 – 53439Cytoplasmic Potential

Amino acid modifications

Modified residue271Phosphoserine Ref.7
Cross-link283Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Sequences

Sequence LengthMass (Da)Tools
P53322 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: ADE7C1DE793FDAA2

FASTA53460,135
        10         20         30         40         50         60 
MSNKFTMESP KHLVDDVLFI SPTNDGSEEK PTEVTFQEDE GHDASLHNRS HDKKSELATE 

        70         80         90        100        110        120 
REIMATTTDD DGIPSPSHPM EKRVLRKMDI YLIPLMGMLY FLSNLDKSNI GNAEVAGLSK 

       130        140        150        160        170        180 
DIHLVGTQYN TCVTVFFATY VLFDPIGTNL LKIMGPPLMM SICLTCFGAI SLGTAWVKNY 

       190        200        210        220        230        240 
AQLIVVRLLL GAFEGMIYPA INMYLSVCYR REQYALRFAF VFSAACLSSS FGGLIAYGCS 

       250        260        270        280        290        300 
KISGSLKDWQ YIYIVEGCIS LGFVPFYAFG LSKNLEDSWF FNKEEKEYIS ERYKTMNTFD 

       310        320        330        340        350        360 
PDEKFEWFQV WQAVKDVKTW ASAVALFGID LTTFGLTVFL PIIITSMGFT NVRAQLMTVP 

       370        380        390        400        410        420 
IYFLTAIVFF ICAVWSDRIK LRSPFILGAC LTTSIGIAIV LGSQVHGVRY FGVYILCMGI 

       430        440        450        460        470        480 
YVNAACNCLW LSGNTGNYFK RATALGINLF FGSGSGLVSG QIFVAKDKPR YIKGLSISLA 

       490        500        510        520        530 
FQVFSIFMTV VQIFLYKREN DKKKAIIDRC NELGEPIPYD ERLSDKNPEF KYMY 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
Clemente M.L., Sartori G., Cardazzo B., Carignani G.
Yeast 13:287-290(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Transcriptional regulation of the Saccharomyces cerevisiae DAL5 gene family and identification of the high affinity nicotinic acid permease TNA1 (YGR260w)."
Llorente B., Dujon B.
FEBS Lett. 475:237-241(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-283.
Strain: SUB592.
[6]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y07777 Genomic DNA. Translation: CAA69082.1.
Z73044 Genomic DNA. Translation: CAA97289.1.
BK006941 Genomic DNA. Translation: DAA08350.1.
PIRS64593.
RefSeqNP_011776.1. NM_001181389.1.

3D structure databases

ProteinModelPortalP53322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33511. 87 interactions.
DIPDIP-8004N.
MINTMINT-4488034.
STRING4932.YGR260W.

Protein family/group databases

TCDB2.A.1.14.11. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBP53322.
PaxDbP53322.
PeptideAtlasP53322.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR260W; YGR260W; YGR260W.
GeneID853175.
KEGGsce:YGR260W.

Organism-specific databases

CYGDYGR260w.
SGDS000003492. TNA1.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00620000088258.
HOGENOMHOG000167306.
OMAAINMYLS.
OrthoDBEOG7S4XG9.

Enzyme and pathway databases

BioCycYEAST:G3O-30929-MONOMER.

Gene expression databases

GenevestigatorP53322.

Family and domain databases

InterProIPR011701. MFS.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
ProtoNetSearch...

Other

NextBio973305.

Entry information

Entry nameTNA1_YEAST
AccessionPrimary (citable) accession number: P53322
Secondary accession number(s): D6VV39
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families