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Protein

High-affinity nicotinic acid transporter

Gene

TNA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the uptake of nicotinic acid.

GO - Molecular functioni

  • nicotinamide mononucleotide transmembrane transporter activity Source: SGD

GO - Biological processi

  • nicotinamide mononucleotide transmembrane transport Source: GOC
  • nicotinamide mononucleotide transport Source: SGD
  • quinolinic acid transmembrane transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30929-MONOMER.

Protein family/group databases

TCDBi2.A.1.14.11. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity nicotinic acid transporter
Alternative name(s):
Nicotinic acid permease
Gene namesi
Name:TNA1
Ordered Locus Names:YGR260W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VII

Organism-specific databases

CYGDiYGR260w.
EuPathDBiFungiDB:YGR260W.
SGDiS000003492. TNA1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 130130ExtracellularSequence AnalysisAdd
BLAST
Transmembranei131 – 15121HelicalSequence AnalysisAdd
BLAST
Topological domaini152 – 1521CytoplasmicSequence Analysis
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Topological domaini174 – 18714ExtracellularSequence AnalysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence AnalysisAdd
BLAST
Topological domaini209 – 2179CytoplasmicSequence Analysis
Transmembranei218 – 23821HelicalSequence AnalysisAdd
BLAST
Topological domaini239 – 25012ExtracellularSequence AnalysisAdd
BLAST
Transmembranei251 – 27121HelicalSequence AnalysisAdd
BLAST
Topological domaini272 – 32352CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei324 – 34421HelicalSequence AnalysisAdd
BLAST
Topological domaini345 – 35511ExtracellularSequence AnalysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence AnalysisAdd
BLAST
Topological domaini377 – 3848CytoplasmicSequence Analysis
Transmembranei385 – 40521HelicalSequence AnalysisAdd
BLAST
Topological domaini406 – 4105ExtracellularSequence Analysis
Transmembranei411 – 43121HelicalSequence AnalysisAdd
BLAST
Topological domaini432 – 44413CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei445 – 46521HelicalSequence AnalysisAdd
BLAST
Topological domaini466 – 4749ExtracellularSequence Analysis
Transmembranei475 – 49521HelicalSequence AnalysisAdd
BLAST
Topological domaini496 – 53439CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534High-affinity nicotinic acid transporterPRO_0000121369Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Phosphoserine1 Publication
Cross-linki283 – 283Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP53322.
PaxDbiP53322.
PeptideAtlasiP53322.

Expressioni

Gene expression databases

GenevestigatoriP53322.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CSG2P352061EBI-2051164,EBI-2051140
ERG11P106141EBI-2051164,EBI-5127
ERP1Q053591EBI-2051164,EBI-6581
ERV25P548371EBI-2051164,EBI-6642
PHO88P382641EBI-2051164,EBI-13350
SHR3Q027741EBI-2051164,EBI-17099
SPF1P399861EBI-2051164,EBI-3128
SWP1Q027951EBI-2051164,EBI-12666

Protein-protein interaction databases

BioGridi33511. 89 interactions.
DIPiDIP-8004N.
MINTiMINT-4488034.
STRINGi4932.YGR260W.

Structurei

3D structure databases

ProteinModelPortaliP53322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00620000088258.
HOGENOMiHOG000167306.
InParanoidiP53322.
OMAiLEDSWFF.
OrthoDBiEOG7S4XG9.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.

Sequencei

Sequence statusi: Complete.

P53322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKFTMESP KHLVDDVLFI SPTNDGSEEK PTEVTFQEDE GHDASLHNRS
60 70 80 90 100
HDKKSELATE REIMATTTDD DGIPSPSHPM EKRVLRKMDI YLIPLMGMLY
110 120 130 140 150
FLSNLDKSNI GNAEVAGLSK DIHLVGTQYN TCVTVFFATY VLFDPIGTNL
160 170 180 190 200
LKIMGPPLMM SICLTCFGAI SLGTAWVKNY AQLIVVRLLL GAFEGMIYPA
210 220 230 240 250
INMYLSVCYR REQYALRFAF VFSAACLSSS FGGLIAYGCS KISGSLKDWQ
260 270 280 290 300
YIYIVEGCIS LGFVPFYAFG LSKNLEDSWF FNKEEKEYIS ERYKTMNTFD
310 320 330 340 350
PDEKFEWFQV WQAVKDVKTW ASAVALFGID LTTFGLTVFL PIIITSMGFT
360 370 380 390 400
NVRAQLMTVP IYFLTAIVFF ICAVWSDRIK LRSPFILGAC LTTSIGIAIV
410 420 430 440 450
LGSQVHGVRY FGVYILCMGI YVNAACNCLW LSGNTGNYFK RATALGINLF
460 470 480 490 500
FGSGSGLVSG QIFVAKDKPR YIKGLSISLA FQVFSIFMTV VQIFLYKREN
510 520 530
DKKKAIIDRC NELGEPIPYD ERLSDKNPEF KYMY
Length:534
Mass (Da):60,135
Last modified:October 1, 1996 - v1
Checksum:iADE7C1DE793FDAA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07777 Genomic DNA. Translation: CAA69082.1.
Z73044 Genomic DNA. Translation: CAA97289.1.
BK006941 Genomic DNA. Translation: DAA08350.1.
PIRiS64593.
RefSeqiNP_011776.1. NM_001181389.1.

Genome annotation databases

EnsemblFungiiYGR260W; YGR260W; YGR260W.
GeneIDi853175.
KEGGisce:YGR260W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07777 Genomic DNA. Translation: CAA69082.1.
Z73044 Genomic DNA. Translation: CAA97289.1.
BK006941 Genomic DNA. Translation: DAA08350.1.
PIRiS64593.
RefSeqiNP_011776.1. NM_001181389.1.

3D structure databases

ProteinModelPortaliP53322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33511. 89 interactions.
DIPiDIP-8004N.
MINTiMINT-4488034.
STRINGi4932.YGR260W.

Protein family/group databases

TCDBi2.A.1.14.11. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBiP53322.
PaxDbiP53322.
PeptideAtlasiP53322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR260W; YGR260W; YGR260W.
GeneIDi853175.
KEGGisce:YGR260W.

Organism-specific databases

CYGDiYGR260w.
EuPathDBiFungiDB:YGR260W.
SGDiS000003492. TNA1.

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00620000088258.
HOGENOMiHOG000167306.
InParanoidiP53322.
OMAiLEDSWFF.
OrthoDBiEOG7S4XG9.

Enzyme and pathway databases

BioCyciYEAST:G3O-30929-MONOMER.

Miscellaneous databases

NextBioi973305.
PROiP53322.

Gene expression databases

GenevestigatoriP53322.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of an 11.6 kb region from the right arm of chromosome VII of Saccharomyces cerevisiae between the RAD2 and the MES1 genes reveals the presence of three new genes."
    Clemente M.L., Sartori G., Cardazzo B., Carignani G.
    Yeast 13:287-290(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Transcriptional regulation of the Saccharomyces cerevisiae DAL5 gene family and identification of the high affinity nicotinic acid permease TNA1 (YGR260w)."
    Llorente B., Dujon B.
    FEBS Lett. 475:237-241(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-283.
    Strain: SUB592.
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTNA1_YEAST
AccessioniPrimary (citable) accession number: P53322
Secondary accession number(s): D6VV39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 27, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.