SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P53320

- MTM1_YEAST

UniProt

P53320 - MTM1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Mitochondrial carrier protein MTM1

Gene
MTM1, YGR257C, G9175
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the mitochondrial activation of SOD2 by specifically facilitating insertion of the essential manganese cofactor. Has the ability to activate iron regulon in an iron-dependent manner. Responds to calorie restriction (CR) strength.3 Publications

GO - Molecular functioni

  1. metallochaperone activity Source: SGD
  2. pyridoxal phosphate binding Source: SGD

GO - Biological processi

  1. manganese ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30927-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial carrier protein MTM1
Alternative name(s):
Manganese trafficking factor for mitochondrial SOD2
Gene namesi
Name:MTM1
Ordered Locus Names:YGR257C
ORF Names:G9175
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR257c.
SGDiS000003489. MTM1.

Subcellular locationi

Mitochondrion inner membrane; Multi-pass membrane protein Reviewed prediction 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei17 – 3620Helical; Name=1; Reviewed predictionAdd
BLAST
Transmembranei126 – 14621Helical; Name=2; Reviewed predictionAdd
BLAST
Transmembranei162 – 18221Helical; Name=3; Reviewed predictionAdd
BLAST
Transmembranei229 – 24921Helical; Name=4; Reviewed predictionAdd
BLAST
Transmembranei268 – 28619Helical; Name=5; Reviewed predictionAdd
BLAST
Transmembranei331 – 35222Helical; Name=6; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: UniProtKB-SubCell
  3. mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Loss of mitochondrial manganese superoxide dismutase SOD2 activity due to misincorporation of iron into SOD2 rather than manganese. Normal SOD2 activity when in association with YFH1 deletion. Doesn't impair activity of a cytosolic version of manganese SOD. The iron regulatory transcription factor AFT1 is constitutively active. Accumulates mtDNA mutations. Elevated mitochondrial iron and manganese levels.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Mitochondrial carrier protein MTM1PRO_0000090697Add
BLAST

Proteomic databases

MaxQBiP53320.
PaxDbiP53320.
PRIDEiP53320.

Expressioni

Gene expression databases

GenevestigatoriP53320.

Interactioni

Protein-protein interaction databases

BioGridi33509. 10 interactions.
DIPiDIP-5452N.
IntActiP53320. 1 interaction.
MINTiMINT-559667.
STRINGi4932.YGR257C.

Structurei

3D structure databases

ProteinModelPortaliP53320.
SMRiP53320. Positions 15-364.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati14 – 149136Solcar 1Add
BLAST
Repeati156 – 25095Solcar 2Add
BLAST
Repeati266 – 35994Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG276989.
GeneTreeiENSGT00390000000106.
HOGENOMiHOG000214473.
KOiK15119.
OMAiLETIWRT.
OrthoDBiEOG7SBNZS.

Family and domain databases

Gene3Di1.50.40.10. 2 hits.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 2 hits.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P53320-1 [UniParc]FASTAAdd to Basket

« Hide

MSDRNTSNSL TLKERMLSAG AGSVLTSLIL TPMDVVRIRL QQQQMIPDCS    50
CDGAAEVPNA VSSGSKMKTF TNVGGQNLNN AKIFWESACF QELHCKNSSL 100
KFNGTLEAFT KIASVEGITS LWRGISLTLL MAIPANMVYF SGYEYIRDVS 150
PIASTYPTLN PLFCGAIARV FAATSIAPLE LVKTKLQSIP RSSKSTKTWM 200
MVKDLLNETR QEMKMVGPSR ALFKGLEITL WRDVPFSAIY WSSYELCKER 250
LWLDSTRFAS KDANWVHFIN SFASGCISGM IAAICTHPFD VGKTRWQISM 300
MNNSDPKGGN RSRNMFKFLE TIWRTEGLAA LYTGLAARVI KIRPSCAIMI 350
SSYEISKKVF GNKLHQ 366
Length:366
Mass (Da):40,763
Last modified:October 1, 1996 - v1
Checksum:iB0358B6EE818CB1E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99228 Genomic DNA. Translation: CAA67613.1.
Z73042 Genomic DNA. Translation: CAA97286.1.
BK006941 Genomic DNA. Translation: DAA08348.1.
PIRiS64589.
RefSeqiNP_011773.3. NM_001181386.3.

Genome annotation databases

EnsemblFungiiYGR257C; YGR257C; YGR257C.
GeneIDi853173.
KEGGisce:YGR257C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X99228 Genomic DNA. Translation: CAA67613.1 .
Z73042 Genomic DNA. Translation: CAA97286.1 .
BK006941 Genomic DNA. Translation: DAA08348.1 .
PIRi S64589.
RefSeqi NP_011773.3. NM_001181386.3.

3D structure databases

ProteinModelPortali P53320.
SMRi P53320. Positions 15-364.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33509. 10 interactions.
DIPi DIP-5452N.
IntActi P53320. 1 interaction.
MINTi MINT-559667.
STRINGi 4932.YGR257C.

Proteomic databases

MaxQBi P53320.
PaxDbi P53320.
PRIDEi P53320.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR257C ; YGR257C ; YGR257C .
GeneIDi 853173.
KEGGi sce:YGR257C.

Organism-specific databases

CYGDi YGR257c.
SGDi S000003489. MTM1.

Phylogenomic databases

eggNOGi NOG276989.
GeneTreei ENSGT00390000000106.
HOGENOMi HOG000214473.
KOi K15119.
OMAi LETIWRT.
OrthoDBi EOG7SBNZS.

Enzyme and pathway databases

BioCyci YEAST:G3O-30927-MONOMER.

Miscellaneous databases

NextBioi 973299.
PROi P53320.

Gene expression databases

Genevestigatori P53320.

Family and domain databases

Gene3Di 1.50.40.10. 2 hits.
InterProi IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view ]
Pfami PF00153. Mito_carr. 3 hits.
[Graphical view ]
SUPFAMi SSF103506. SSF103506. 2 hits.
PROSITEi PS50920. SOLCAR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene."
    Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L.
    Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Manganese activation of superoxide dismutase 2 in Saccharomyces cerevisiae requires MTM1, a member of the mitochondrial carrier family."
    Luk E., Carroll M., Baker M., Culotta V.C.
    Proc. Natl. Acad. Sci. U.S.A. 100:10353-10357(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  6. "The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2."
    Yang M., Cobine P.A., Molik S., Naranuntarat A., Lill R., Winge D.R., Culotta V.C.
    EMBO J. 25:1775-1783(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis."
    Kumanovics A., Chen O.S., Li L., Bagley D., Adkins E.M., Lin H., Dingra N.N., Outten C.E., Keller G., Winge D., Ward D.M., Kaplan J.
    J. Biol. Chem. 283:10276-10286(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Transcriptional response according to strength of calorie restriction in Saccharomyces cerevisiae."
    Lee Y.-L., Lee C.-K.
    Mol. Cells 26:299-307(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMTM1_YEAST
AccessioniPrimary (citable) accession number: P53320
Secondary accession number(s): D6VV37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3

Similar proteinsi