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P53320

- MTM1_YEAST

UniProt

P53320 - MTM1_YEAST

Protein

Mitochondrial carrier protein MTM1

Gene

MTM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Involved in the mitochondrial activation of SOD2 by specifically facilitating insertion of the essential manganese cofactor. Has the ability to activate iron regulon in an iron-dependent manner. Responds to calorie restriction (CR) strength.3 Publications

    GO - Molecular functioni

    1. metallochaperone activity Source: SGD
    2. pyridoxal phosphate binding Source: SGD

    GO - Biological processi

    1. manganese ion transport Source: SGD

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30927-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial carrier protein MTM1
    Alternative name(s):
    Manganese trafficking factor for mitochondrial SOD2
    Gene namesi
    Name:MTM1
    Ordered Locus Names:YGR257C
    ORF Names:G9175
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR257c.
    SGDiS000003489. MTM1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: UniProtKB-SubCell
    3. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Disruption phenotypei

    Loss of mitochondrial manganese superoxide dismutase SOD2 activity due to misincorporation of iron into SOD2 rather than manganese. Normal SOD2 activity when in association with YFH1 deletion. Doesn't impair activity of a cytosolic version of manganese SOD. The iron regulatory transcription factor AFT1 is constitutively active. Accumulates mtDNA mutations. Elevated mitochondrial iron and manganese levels.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Mitochondrial carrier protein MTM1PRO_0000090697Add
    BLAST

    Proteomic databases

    MaxQBiP53320.
    PaxDbiP53320.
    PRIDEiP53320.

    Expressioni

    Gene expression databases

    GenevestigatoriP53320.

    Interactioni

    Protein-protein interaction databases

    BioGridi33509. 10 interactions.
    DIPiDIP-5452N.
    IntActiP53320. 1 interaction.
    MINTiMINT-559667.
    STRINGi4932.YGR257C.

    Structurei

    3D structure databases

    ProteinModelPortaliP53320.
    SMRiP53320. Positions 15-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei17 – 3620Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei126 – 14621Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei162 – 18221Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei229 – 24921Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei268 – 28619Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei331 – 35222Helical; Name=6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati14 – 149136Solcar 1Add
    BLAST
    Repeati156 – 25095Solcar 2Add
    BLAST
    Repeati266 – 35994Solcar 3Add
    BLAST

    Sequence similaritiesi

    Contains 3 Solcar repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG276989.
    GeneTreeiENSGT00390000000106.
    HOGENOMiHOG000214473.
    KOiK15119.
    OMAiLETIWRT.
    OrthoDBiEOG7SBNZS.

    Family and domain databases

    Gene3Di1.50.40.10. 2 hits.
    InterProiIPR018108. Mitochondrial_sb/sol_carrier.
    IPR023395. Mt_carrier_dom.
    [Graphical view]
    PfamiPF00153. Mito_carr. 3 hits.
    [Graphical view]
    SUPFAMiSSF103506. SSF103506. 2 hits.
    PROSITEiPS50920. SOLCAR. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53320-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDRNTSNSL TLKERMLSAG AGSVLTSLIL TPMDVVRIRL QQQQMIPDCS    50
    CDGAAEVPNA VSSGSKMKTF TNVGGQNLNN AKIFWESACF QELHCKNSSL 100
    KFNGTLEAFT KIASVEGITS LWRGISLTLL MAIPANMVYF SGYEYIRDVS 150
    PIASTYPTLN PLFCGAIARV FAATSIAPLE LVKTKLQSIP RSSKSTKTWM 200
    MVKDLLNETR QEMKMVGPSR ALFKGLEITL WRDVPFSAIY WSSYELCKER 250
    LWLDSTRFAS KDANWVHFIN SFASGCISGM IAAICTHPFD VGKTRWQISM 300
    MNNSDPKGGN RSRNMFKFLE TIWRTEGLAA LYTGLAARVI KIRPSCAIMI 350
    SSYEISKKVF GNKLHQ 366
    Length:366
    Mass (Da):40,763
    Last modified:October 1, 1996 - v1
    Checksum:iB0358B6EE818CB1E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99228 Genomic DNA. Translation: CAA67613.1.
    Z73042 Genomic DNA. Translation: CAA97286.1.
    BK006941 Genomic DNA. Translation: DAA08348.1.
    PIRiS64589.
    RefSeqiNP_011773.3. NM_001181386.3.

    Genome annotation databases

    EnsemblFungiiYGR257C; YGR257C; YGR257C.
    GeneIDi853173.
    KEGGisce:YGR257C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99228 Genomic DNA. Translation: CAA67613.1 .
    Z73042 Genomic DNA. Translation: CAA97286.1 .
    BK006941 Genomic DNA. Translation: DAA08348.1 .
    PIRi S64589.
    RefSeqi NP_011773.3. NM_001181386.3.

    3D structure databases

    ProteinModelPortali P53320.
    SMRi P53320. Positions 15-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33509. 10 interactions.
    DIPi DIP-5452N.
    IntActi P53320. 1 interaction.
    MINTi MINT-559667.
    STRINGi 4932.YGR257C.

    Proteomic databases

    MaxQBi P53320.
    PaxDbi P53320.
    PRIDEi P53320.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR257C ; YGR257C ; YGR257C .
    GeneIDi 853173.
    KEGGi sce:YGR257C.

    Organism-specific databases

    CYGDi YGR257c.
    SGDi S000003489. MTM1.

    Phylogenomic databases

    eggNOGi NOG276989.
    GeneTreei ENSGT00390000000106.
    HOGENOMi HOG000214473.
    KOi K15119.
    OMAi LETIWRT.
    OrthoDBi EOG7SBNZS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30927-MONOMER.

    Miscellaneous databases

    NextBioi 973299.
    PROi P53320.

    Gene expression databases

    Genevestigatori P53320.

    Family and domain databases

    Gene3Di 1.50.40.10. 2 hits.
    InterProi IPR018108. Mitochondrial_sb/sol_carrier.
    IPR023395. Mt_carrier_dom.
    [Graphical view ]
    Pfami PF00153. Mito_carr. 3 hits.
    [Graphical view ]
    SUPFAMi SSF103506. SSF103506. 2 hits.
    PROSITEi PS50920. SOLCAR. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene."
      Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L.
      Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Manganese activation of superoxide dismutase 2 in Saccharomyces cerevisiae requires MTM1, a member of the mitochondrial carrier family."
      Luk E., Carroll M., Baker M., Culotta V.C.
      Proc. Natl. Acad. Sci. U.S.A. 100:10353-10357(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    6. "The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2."
      Yang M., Cobine P.A., Molik S., Naranuntarat A., Lill R., Winge D.R., Culotta V.C.
      EMBO J. 25:1775-1783(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis."
      Kumanovics A., Chen O.S., Li L., Bagley D., Adkins E.M., Lin H., Dingra N.N., Outten C.E., Keller G., Winge D., Ward D.M., Kaplan J.
      J. Biol. Chem. 283:10276-10286(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Transcriptional response according to strength of calorie restriction in Saccharomyces cerevisiae."
      Lee Y.-L., Lee C.-K.
      Mol. Cells 26:299-307(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiMTM1_YEAST
    AccessioniPrimary (citable) accession number: P53320
    Secondary accession number(s): D6VV37
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 538 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3