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P53320 (MTM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial carrier protein MTM1
Alternative name(s):
Manganese trafficking factor for mitochondrial SOD2
Gene names
Name:MTM1
Ordered Locus Names:YGR257C
ORF Names:G9175
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the mitochondrial activation of SOD2 by specifically facilitating insertion of the essential manganese cofactor. Has the ability to activate iron regulon in an iron-dependent manner. Responds to calorie restriction (CR) strength. Ref.5 Ref.7 Ref.8

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.5.

Disruption phenotype

Loss of mitochondrial manganese superoxide dismutase SOD2 activity due to misincorporation of iron into SOD2 rather than manganese. Normal SOD2 activity when in association with YFH1 deletion. Doesn't impair activity of a cytosolic version of manganese SOD. The iron regulatory transcription factor AFT1 is constitutively active. Accumulates mtDNA mutations. Elevated mitochondrial iron and manganese levels. Ref.5 Ref.6

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]

Contains 3 Solcar repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Mitochondrial carrier protein MTM1
PRO_0000090697

Regions

Transmembrane17 – 3620Helical; Name=1; Potential
Transmembrane126 – 14621Helical; Name=2; Potential
Transmembrane162 – 18221Helical; Name=3; Potential
Transmembrane229 – 24921Helical; Name=4; Potential
Transmembrane268 – 28619Helical; Name=5; Potential
Transmembrane331 – 35222Helical; Name=6; Potential
Repeat14 – 149136Solcar 1
Repeat156 – 25095Solcar 2
Repeat266 – 35994Solcar 3

Sequences

Sequence LengthMass (Da)Tools
P53320 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B0358B6EE818CB1E

FASTA36640,763
        10         20         30         40         50         60 
MSDRNTSNSL TLKERMLSAG AGSVLTSLIL TPMDVVRIRL QQQQMIPDCS CDGAAEVPNA 

        70         80         90        100        110        120 
VSSGSKMKTF TNVGGQNLNN AKIFWESACF QELHCKNSSL KFNGTLEAFT KIASVEGITS 

       130        140        150        160        170        180 
LWRGISLTLL MAIPANMVYF SGYEYIRDVS PIASTYPTLN PLFCGAIARV FAATSIAPLE 

       190        200        210        220        230        240 
LVKTKLQSIP RSSKSTKTWM MVKDLLNETR QEMKMVGPSR ALFKGLEITL WRDVPFSAIY 

       250        260        270        280        290        300 
WSSYELCKER LWLDSTRFAS KDANWVHFIN SFASGCISGM IAAICTHPFD VGKTRWQISM 

       310        320        330        340        350        360 
MNNSDPKGGN RSRNMFKFLE TIWRTEGLAA LYTGLAARVI KIRPSCAIMI SSYEISKKVF 


GNKLHQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene."
Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L.
Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Manganese activation of superoxide dismutase 2 in Saccharomyces cerevisiae requires MTM1, a member of the mitochondrial carrier family."
Luk E., Carroll M., Baker M., Culotta V.C.
Proc. Natl. Acad. Sci. U.S.A. 100:10353-10357(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]"The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2."
Yang M., Cobine P.A., Molik S., Naranuntarat A., Lill R., Winge D.R., Culotta V.C.
EMBO J. 25:1775-1783(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis."
Kumanovics A., Chen O.S., Li L., Bagley D., Adkins E.M., Lin H., Dingra N.N., Outten C.E., Keller G., Winge D., Ward D.M., Kaplan J.
J. Biol. Chem. 283:10276-10286(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Transcriptional response according to strength of calorie restriction in Saccharomyces cerevisiae."
Lee Y.-L., Lee C.-K.
Mol. Cells 26:299-307(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99228 Genomic DNA. Translation: CAA67613.1.
Z73042 Genomic DNA. Translation: CAA97286.1.
BK006941 Genomic DNA. Translation: DAA08348.1.
PIRS64589.
RefSeqNP_011773.3. NM_001181386.3.

3D structure databases

ProteinModelPortalP53320.
SMRP53320. Positions 15-364.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33509. 10 interactions.
DIPDIP-5452N.
IntActP53320. 1 interaction.
MINTMINT-559667.
STRING4932.YGR257C.

Proteomic databases

PaxDbP53320.
PRIDEP53320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR257C; YGR257C; YGR257C.
GeneID853173.
KEGGsce:YGR257C.

Organism-specific databases

CYGDYGR257c.
SGDS000003489. MTM1.

Phylogenomic databases

eggNOGNOG276989.
GeneTreeENSGT00390000000106.
HOGENOMHOG000214473.
KOK15119.
OMANERHHAS.
OrthoDBEOG7SBNZS.

Enzyme and pathway databases

BioCycYEAST:G3O-30927-MONOMER.

Gene expression databases

GenevestigatorP53320.

Family and domain databases

Gene3D1.50.40.10. 2 hits.
InterProIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMSSF103506. SSF103506. 2 hits.
PROSITEPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio973299.
PROP53320.

Entry information

Entry nameMTM1_YEAST
AccessionPrimary (citable) accession number: P53320
Secondary accession number(s): D6VV37
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 19, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families