P53320 (MTM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial carrier protein MTM1

Alternative name(s):
Manganese trafficking factor for mitochondrial SOD2

Gene names

Name:	MTM1
Ordered Locus Names:	YGR257C
ORF Names:	G9175

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the mitochondrial activation of SOD2 by specifically facilitating insertion of the essential manganese cofactor. Has the ability to activate iron regulon in an iron-dependent manner. Responds to calorie restriction (CR) strength. Ref.5 Ref.7 Ref.8
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Potential Ref.5.
Disruption phenotype	Loss of mitochondrial manganese superoxide dismutase SOD2 activity due to misincorporation of iron into SOD2 rather than manganese. Normal SOD2 activity when in association with YFH1 deletion. Doesn't impair activity of a cytosolic version of manganese SOD. The iron regulatory transcription factor AFT1 is constitutively active. Accumulates mtDNA mutations. Elevated mitochondrial iron and manganese levels. Ref.5 Ref.6
Miscellaneous	Present with 538 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification] Contains 3 Solcar repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	manganese ion transport Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrial inner membrane Inferred from sequence or structural similarity PubMed 10930523. Source: SGD
Molecular_function	metallochaperone activity Inferred from mutant phenotype Ref.5. Source: SGD transporter activity Inferred from sequence or structural similarity PubMed 10930523. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Mitochondrial carrier protein MTM1		PRO_0000090697
Regions
	Transmembrane	17 – 36	20	Helical; Name=1; Potential
	Transmembrane	126 – 146	21	Helical; Name=2; Potential
	Transmembrane	162 – 182	21	Helical; Name=3; Potential
	Transmembrane	229 – 249	21	Helical; Name=4; Potential
	Transmembrane	268 – 286	19	Helical; Name=5; Potential
	Transmembrane	331 – 352	22	Helical; Name=6; Potential
	Repeat	14 – 149	136	Solcar 1
	Repeat	156 – 250	95	Solcar 2
	Repeat	266 – 359	94	Solcar 3

Sequences

	Sequence		Length	Mass (Da)
	P53320 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B0358B6EE818CB1E	FASTA	366	40,763
	10 20 30 40 50 60 MSDRNTSNSL TLKERMLSAG AGSVLTSLIL TPMDVVRIRL QQQQMIPDCS CDGAAEVPNA 70 80 90 100 110 120 VSSGSKMKTF TNVGGQNLNN AKIFWESACF QELHCKNSSL KFNGTLEAFT KIASVEGITS 130 140 150 160 170 180 LWRGISLTLL MAIPANMVYF SGYEYIRDVS PIASTYPTLN PLFCGAIARV FAATSIAPLE 190 200 210 220 230 240 LVKTKLQSIP RSSKSTKTWM MVKDLLNETR QEMKMVGPSR ALFKGLEITL WRDVPFSAIY 250 260 270 280 290 300 WSSYELCKER LWLDSTRFAS KDANWVHFIN SFASGCISGM IAAICTHPFD VGKTRWQISM 310 320 330 340 350 360 MNNSDPKGGN RSRNMFKFLE TIWRTEGLAA LYTGLAARVI KIRPSCAIMI SSYEISKKVF GNKLHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene." Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L. Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Manganese activation of superoxide dismutase 2 in Saccharomyces cerevisiae requires MTM1, a member of the mitochondrial carrier family." Luk E., Carroll M., Baker M., Culotta V.C. Proc. Natl. Acad. Sci. U.S.A. 100:10353-10357(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[6]	"The effects of mitochondrial iron homeostasis on cofactor specificity of superoxide dismutase 2." Yang M., Cobine P.A., Molik S., Naranuntarat A., Lill R., Winge D.R., Culotta V.C. EMBO J. 25:1775-1783(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"Identification of FRA1 and FRA2 as genes involved in regulating the yeast iron regulon in response to decreased mitochondrial iron-sulfur cluster synthesis." Kumanovics A., Chen O.S., Li L., Bagley D., Adkins E.M., Lin H., Dingra N.N., Outten C.E., Keller G., Winge D., Ward D.M., Kaplan J. J. Biol. Chem. 283:10276-10286(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Transcriptional response according to strength of calorie restriction in Saccharomyces cerevisiae." Lee Y.-L., Lee C.-K. Mol. Cells 26:299-307(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X99228 Genomic DNA. Translation: CAA67613.1. Z73042 Genomic DNA. Translation: CAA97286.1. BK006941 Genomic DNA. Translation: DAA08348.1.
PIR	S64589.
RefSeq	NP_011773.3. NM_001181386.3.
3D structure databases
ProteinModelPortal	P53320.
SMR	P53320. Positions 15-360.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5452N.
MINT	MINT-559667.
STRING	4932.YGR257C.
Proteomic databases
PaxDb	P53320.
PRIDE	P53320.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR257C; YGR257C; YGR257C.
GeneID	853173.
KEGG	sce:YGR257C. sce:YGR264C.
Organism-specific databases
CYGD	YGR257c.
SGD	S000003489. MTM1.
Phylogenomic databases
eggNOG	NOG276989.
GeneTree	ENSGT00390000000106.
HOGENOM	HOG000214473.
KO	K01874. K15119.
OMA	LETIWRT.
OrthoDB	EOG4936TF.
Gene expression databases
Genevestigator	P53320.
GermOnline	YGR257C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.50.40.10. 2 hits.
InterPro	IPR018108. Mitochondrial_sb/sol_carrier. IPR023395. Mt_carrier_dom. [Graphical view]
Pfam	PF00153. Mito_carr. 3 hits. [Graphical view]
SUPFAM	SSF103506. Mitoch_carrier. 1 hit.
PROSITE	PS50920. SOLCAR. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	973299.

Entry information

Entry name

MTM1_YEAST

Accession

Primary (citable) accession number: P53320
Secondary accession number(s): D6VV37

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program