ID   6PGD2_YEAST             Reviewed;         492 AA.
AC   P53319;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   16-JUN-2009, entry version 79.
DE   RecName: Full=6-phosphogluconate dehydrogenase, decarboxylating 2;
DE            EC=1.1.1.44;
GN   Name=GND2; OrderedLocusNames=YGR256W; ORFNames=G9170;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=97279233; PubMed=9133741;
RX   DOI=10.1002/(SICI)1097-0061(19970330)13:4<369::AID-YEA81>3.3.CO;2-M;
RA   Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F.,
RA   Frontali L.;
RT   "Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome
RT   VII reveals the presence of three new open reading frames and of a
RT   tRNAThr gene.";
RL   Yeast 13:369-372(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   MEDLINE=97313265; PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M.,
RA   Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J.,
RA   Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E.,
RA   Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E.,
RA   Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B.,
RA   Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L.,
RA   Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M.,
RA   Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M.,
RA   Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B.,
RA   Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W.,
RA   Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A.,
RA   Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S.,
RA   Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L.,
RA   Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S.,
RA   Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J.,
RA   Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M.,
RA   Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B.,
RA   Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J.,
RA   Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M.,
RA   van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M.,
RA   Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H.,
RA   Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M.,
RA   Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A.,
RA   Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F.,
RA   Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G.,
RA   Kolodner R.D., LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-
RT   encoding clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A.,
RA   Dephoure N., O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- CATALYTIC ACTIVITY: 6-phospho-D-gluconate + NADP(+) = D-ribulose
CC       5-phosphate + CO(2) + NADPH.
CC   -!- PATHWAY: Carbohydrate degradation; pentose phosphate pathway; D-
CC       ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage):
CC       step 3/3.
CC   -!- INTERACTION:
CC       Self; NbExp=1; IntAct=EBI-1972, EBI-1972;
CC   -!- MISCELLANEOUS: Present with 556 molecules/cell in log phase SD
CC       medium.
CC   -!- SIMILARITY: Belongs to the 6-phosphogluconate dehydrogenase
CC       family.
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DR   EMBL; X99228; CAA67612.1; -; Genomic_DNA.
DR   EMBL; Z73041; CAA97285.1; -; Genomic_DNA.
DR   EMBL; AY692811; AAT92830.1; -; Genomic_DNA.
DR   PIR; S64588; S64588.
DR   RefSeq; NP_011772.1; -.
DR   HSSP; P00349; 2PGD.
DR   DIP; DIP:6605N; -.
DR   IntAct; P53319; 6.
DR   PeptideAtlas; P53319; -.
DR   Ensembl; YGR256W; Saccharomyces cerevisiae.
DR   GeneID; 853172; -.
DR   GenomeReviews; Y13135_GR; YGR256W.
DR   KEGG; sce:YGR256W; -.
DR   NMPDR; fig|4932.3.peg.2901; -.
DR   CYGD; YGR256w; -.
DR   SGD; S000003488; GND2.
DR   HOGENOM; P53319; -.
DR   OMA; P53319; PECANEN.
DR   BRENDA; 1.1.1.44; 250.
DR   NextBio; 973296; -.
DR   ArrayExpress; P53319; -.
DR   GermOnline; YGR256W; Saccharomyces cerevisiae.
DR   GO; GO:0005829; C:cytosol; TAS:SGD.
DR   GO; GO:0001950; C:plasma membrane enriched fraction; IDA:SGD.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0004616; F:phosphogluconate dehydrogenase (decarboxyla...; IMP:SGD.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0009051; P:pentose-phosphate shunt, oxidative branch; IGI:SGD.
DR   InterPro; IPR006183; 6-phosphogluconate_DH.
DR   InterPro; IPR006114; 6PGDH_C.
DR   InterPro; IPR006113; 6PGDH_decarbox.
DR   InterPro; IPR006115; 6PGDH_NAD-bd.
DR   InterPro; IPR006184; 6PGdom_BS.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR012284; Fibritin/6PGD_C-extension.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:1.20.5.320; Fibritin/6PGD_C-extension; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   Pfam; PF00393; 6PGD; 1.
DR   Pfam; PF03446; NAD_binding_2; 1.
DR   PRINTS; PR00076; 6PGDHDRGNASE.
DR   TIGRFAMs; TIGR00873; gnd; 1.
DR   PROSITE; PS00461; 6PGD; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; NADP; Oxidoreductase; Pentose shunt.
FT   CHAIN         1    492       6-phosphogluconate dehydrogenase,
FT                                decarboxylating 2.
FT                                /FTId=PRO_0000090076.
SQ   SEQUENCE   492 AA;  53923 MW;  3D75D53563987735 CRC64;
     MSKAVGDLGL VGLAVMGQNL ILNAADHGFT VVAYNRTQSK VDRFLANEAK GKSIIGATSI
     EDLVAKLKKP RKIMLLIKAG APVDTLIKEL VPHLDKGDII IDGGNSHFPD TNRRYEELTK
     QGILFVGSGV SGGEDGARFG PSLMPGGSAE AWPHIKNIFQ SIAAKSNGEP CCEWVGPAGS
     GHYVKMVHNG IEYGDMQLIC EAYDIMKRIG RFTDKEISEV FDKWNTGVLD SFLIEITRDI
     LKFDDVDGKP LVEKIMDTAG QKGTGKWTAI NALDLGMPVT LIGEAVFARC LSAIKDERKR
     ASKLLAGPTV PKDAIHDREQ FVYDLEQALY ASKIISYAQG FMLIREAARS YGWKLNNPAI
     ALMWRGGCII RSVFLAEITK AYRDDPDLEN LLFNEFFASA VTKAQSGWRR TIALAATYGI
     PTPAFSTALA FYDGYRSERL PANLLQAQRD YFGAHTFRIL PECASAHLPV DKDIHINWTG
     HGGNISSSTY QA
//