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P53319

- 6PGD2_YEAST

UniProt

P53319 - 6PGD2_YEAST

Protein

6-phosphogluconate dehydrogenase, decarboxylating 2

Gene

GND2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH.By similarity

    Catalytic activityi

    6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei105 – 1051NADPBy similarity
    Binding sitei105 – 1051SubstrateBy similarity
    Active sitei185 – 1851Proton acceptorBy similarity
    Active sitei192 – 1921Proton donorBy similarity
    Binding sitei193 – 1931SubstrateBy similarity
    Binding sitei262 – 2621Substrate; via amide nitrogenBy similarity
    Binding sitei289 – 2891SubstrateBy similarity
    Binding sitei449 – 4491Substrate; shared with dimeric partnerBy similarity
    Binding sitei455 – 4551Substrate; shared with dimeric partnerBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 176NADPBy similarity
    Nucleotide bindingi35 – 373NADPBy similarity
    Nucleotide bindingi77 – 793NADPBy similarity

    GO - Molecular functioni

    1. NADP binding Source: InterPro
    2. phosphogluconate dehydrogenase (decarboxylating) activity Source: SGD

    GO - Biological processi

    1. D-gluconate metabolic process Source: UniProtKB-KW
    2. pentose-phosphate shunt, oxidative branch Source: SGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Gluconate utilization, Pentose shunt

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciYEAST:YGR256W-MONOMER.
    UniPathwayiUPA00115; UER00410.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phosphogluconate dehydrogenase, decarboxylating 2 (EC:1.1.1.44)
    Gene namesi
    Name:GND2
    Ordered Locus Names:YGR256W
    ORF Names:G9170
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGR256w.
    SGDiS000003488. GND2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4924926-phosphogluconate dehydrogenase, decarboxylating 2PRO_0000090076Add
    BLAST

    Proteomic databases

    MaxQBiP53319.
    PaxDbiP53319.
    PeptideAtlasiP53319.
    PRIDEiP53319.

    Expressioni

    Gene expression databases

    GenevestigatoriP53319.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    BioGridi33508. 30 interactions.
    DIPiDIP-6605N.
    IntActiP53319. 1 interaction.
    MINTiMINT-702969.
    STRINGi4932.YGR256W.

    Structurei

    3D structure databases

    ProteinModelPortaliP53319.
    SMRiP53319. Positions 6-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni131 – 1333Substrate bindingBy similarity
    Regioni188 – 1892Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0362.
    GeneTreeiENSGT00390000009023.
    HOGENOMiHOG000255147.
    KOiK00033.
    OMAiPECANET.
    OrthoDBiEOG7F252Z.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    1.20.5.320. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR006114. 6PGDH_C.
    IPR006113. 6PGDH_decarbox.
    IPR006115. 6PGDH_NADP-bd.
    IPR006184. 6PGdom_BS.
    IPR013328. DH_multihelical.
    IPR012284. Fibritin/6PGD_C-extension.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00393. 6PGD. 1 hit.
    PF03446. NAD_binding_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000109. 6PGD. 1 hit.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR00873. gnd. 1 hit.
    PROSITEiPS00461. 6PGD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P53319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKAVGDLGL VGLAVMGQNL ILNAADHGFT VVAYNRTQSK VDRFLANEAK    50
    GKSIIGATSI EDLVAKLKKP RKIMLLIKAG APVDTLIKEL VPHLDKGDII 100
    IDGGNSHFPD TNRRYEELTK QGILFVGSGV SGGEDGARFG PSLMPGGSAE 150
    AWPHIKNIFQ SIAAKSNGEP CCEWVGPAGS GHYVKMVHNG IEYGDMQLIC 200
    EAYDIMKRIG RFTDKEISEV FDKWNTGVLD SFLIEITRDI LKFDDVDGKP 250
    LVEKIMDTAG QKGTGKWTAI NALDLGMPVT LIGEAVFARC LSAIKDERKR 300
    ASKLLAGPTV PKDAIHDREQ FVYDLEQALY ASKIISYAQG FMLIREAARS 350
    YGWKLNNPAI ALMWRGGCII RSVFLAEITK AYRDDPDLEN LLFNEFFASA 400
    VTKAQSGWRR TIALAATYGI PTPAFSTALA FYDGYRSERL PANLLQAQRD 450
    YFGAHTFRIL PECASAHLPV DKDIHINWTG HGGNISSSTY QA 492
    Length:492
    Mass (Da):53,923
    Last modified:October 1, 1996 - v1
    Checksum:i3D75D53563987735
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99228 Genomic DNA. Translation: CAA67612.1.
    Z73041 Genomic DNA. Translation: CAA97285.1.
    AY692811 Genomic DNA. Translation: AAT92830.1.
    BK006941 Genomic DNA. Translation: DAA08347.1.
    PIRiS64588.
    RefSeqiNP_011772.3. NM_001181385.3.

    Genome annotation databases

    EnsemblFungiiYGR256W; YGR256W; YGR256W.
    GeneIDi853172.
    KEGGisce:YGR256W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X99228 Genomic DNA. Translation: CAA67612.1 .
    Z73041 Genomic DNA. Translation: CAA97285.1 .
    AY692811 Genomic DNA. Translation: AAT92830.1 .
    BK006941 Genomic DNA. Translation: DAA08347.1 .
    PIRi S64588.
    RefSeqi NP_011772.3. NM_001181385.3.

    3D structure databases

    ProteinModelPortali P53319.
    SMRi P53319. Positions 6-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33508. 30 interactions.
    DIPi DIP-6605N.
    IntActi P53319. 1 interaction.
    MINTi MINT-702969.
    STRINGi 4932.YGR256W.

    Proteomic databases

    MaxQBi P53319.
    PaxDbi P53319.
    PeptideAtlasi P53319.
    PRIDEi P53319.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGR256W ; YGR256W ; YGR256W .
    GeneIDi 853172.
    KEGGi sce:YGR256W.

    Organism-specific databases

    CYGDi YGR256w.
    SGDi S000003488. GND2.

    Phylogenomic databases

    eggNOGi COG0362.
    GeneTreei ENSGT00390000009023.
    HOGENOMi HOG000255147.
    KOi K00033.
    OMAi PECANET.
    OrthoDBi EOG7F252Z.

    Enzyme and pathway databases

    UniPathwayi UPA00115 ; UER00410 .
    BioCyci YEAST:YGR256W-MONOMER.

    Miscellaneous databases

    NextBioi 973296.
    PROi P53319.

    Gene expression databases

    Genevestigatori P53319.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    1.20.5.320. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR006114. 6PGDH_C.
    IPR006113. 6PGDH_decarbox.
    IPR006115. 6PGDH_NADP-bd.
    IPR006184. 6PGdom_BS.
    IPR013328. DH_multihelical.
    IPR012284. Fibritin/6PGD_C-extension.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00393. 6PGD. 1 hit.
    PF03446. NAD_binding_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000109. 6PGD. 1 hit.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR00873. gnd. 1 hit.
    PROSITEi PS00461. 6PGD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene."
      Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L.
      Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry namei6PGD2_YEAST
    AccessioniPrimary (citable) accession number: P53319
    Secondary accession number(s): D6VV36
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 556 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3