Reviewed, UniProtKB/Swiss-Prot P53319 (6PGD2_YEAST)

Names and origin

Protein names

Recommended name:
    6-phosphogluconate dehydrogenase, decarboxylating 2
    EC=1.1.1.44

Gene names

Name:	GND2
Ordered Locus Names:	YGR256W
ORF Names:	G9170

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.
Miscellaneous	Present with 556 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the 6-phosphogluconate dehydrogenase family.

Ontologies

Keywords
Biological process	Pentose shunt
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt, oxidative branch Inferred from genetic interaction. Source: SGD
Cellular component	cytosol Traceable author statement. Source: SGD plasma membrane enriched fraction Inferred from direct assay. Source: SGD
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro identical protein binding Inferred from physical interaction. Source: IntAct phosphogluconate dehydrogenase (decarboxylating) activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-1972,EBI-1972

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	6-phosphogluconate dehydrogenase, decarboxylating 2		PRO_0000090076

Sequences

	Sequence		Length	Mass (Da)
	P53319-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3D75D53563987735	FASTA	492	53,923
	10 20 30 40 50 60 MSKAVGDLGL VGLAVMGQNL ILNAADHGFT VVAYNRTQSK VDRFLANEAK GKSIIGATSI 70 80 90 100 110 120 EDLVAKLKKP RKIMLLIKAG APVDTLIKEL VPHLDKGDII IDGGNSHFPD TNRRYEELTK 130 140 150 160 170 180 QGILFVGSGV SGGEDGARFG PSLMPGGSAE AWPHIKNIFQ SIAAKSNGEP CCEWVGPAGS 190 200 210 220 230 240 GHYVKMVHNG IEYGDMQLIC EAYDIMKRIG RFTDKEISEV FDKWNTGVLD SFLIEITRDI 250 260 270 280 290 300 LKFDDVDGKP LVEKIMDTAG QKGTGKWTAI NALDLGMPVT LIGEAVFARC LSAIKDERKR 310 320 330 340 350 360 ASKLLAGPTV PKDAIHDREQ FVYDLEQALY ASKIISYAQG FMLIREAARS YGWKLNNPAI 370 380 390 400 410 420 ALMWRGGCII RSVFLAEITK AYRDDPDLEN LLFNEFFASA VTKAQSGWRR TIALAATYGI 430 440 450 460 470 480 PTPAFSTALA FYDGYRSERL PANLLQAQRD YFGAHTFRIL PECASAHLPV DKDIHINWTG 490 HGGNISSSTY QA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene." Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L. Yeast 13:369-372(1997) [PubMed: 9133741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99228 Genomic DNA. Translation: CAA67612.1. Z73041 Genomic DNA. Translation: CAA97285.1. AY692811 Genomic DNA. Translation: AAT92830.1.
PIR	S64588.
RefSeq	NP_011772.1.
3D structure databases
HSSP	HSSP built from PDB template 2PGD based on UniProtKB P00349.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6605N.
IntAct	P53319. 6 interactions.
Proteomic databases
PeptideAtlas	P53319.
Genome annotation databases
Ensembl	YGR256W. Saccharomyces cerevisiae. [Contig view]
GeneID	853172.
GenomeReviews	Gene locus YGR256W in contig Y13135_GR.
KEGG	sce:YGR256W.
NMPDR	fig|4932.3.peg.2901.
Organism-specific databases
CYGD	YGR256w.
SGD	S000003488. GND2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P53319.
OMA	P53319. PECANEN.
Enzyme and pathway databases
BRENDA	1.1.1.44. 250.
Gene expression databases
ArrayExpress	P53319.
GermOnline	YGR256W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006183. 6-phosphogluconate_DH. IPR006114. 6PGDH_C. IPR006113. 6PGDH_decarbox. IPR006115. 6PGDH_NAD-bd. IPR006184. 6PGdom_BS. IPR013328. DH_multihelical. IPR012284. Fibritin/6PGD_C-extension. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:1.20.5.320. Fibritin/6PGD_C-extension. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00393. 6PGD. 1 hit. PF03446. NAD_binding_2. 1 hit. [Graphical view]
PRINTS	PR00076. 6PGDHDRGNASE.
TIGRFAMs	TIGR00873. gnd. 1 hit.
PROSITE	PS00461. 6PGD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973296.

Entry information

Entry name

6PGD2_YEAST

Accession

Primary (citable) accession number: P53319

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)