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P53319 (6PGD2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphogluconate dehydrogenase, decarboxylating 2

EC=1.1.1.44
Gene names
Name:GND2
Ordered Locus Names:YGR256W
ORF Names:G9170
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH By similarity.

Catalytic activity

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.

Subunit structure

Homodimer By similarity.

Miscellaneous

Present with 556 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the 6-phosphogluconate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4924926-phosphogluconate dehydrogenase, decarboxylating 2
PRO_0000090076

Regions

Nucleotide binding12 – 176NADP By similarity
Nucleotide binding35 – 373NADP By similarity
Nucleotide binding77 – 793NADP By similarity
Region131 – 1333Substrate binding By similarity
Region188 – 1892Substrate binding By similarity

Sites

Active site1851Proton acceptor By similarity
Active site1921Proton donor By similarity
Binding site1051NADP By similarity
Binding site1051Substrate By similarity
Binding site1931Substrate By similarity
Binding site2621Substrate; via amide nitrogen By similarity
Binding site2891Substrate By similarity
Binding site4491Substrate; shared with dimeric partner By similarity
Binding site4551Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
P53319 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 3D75D53563987735

FASTA49253,923
        10         20         30         40         50         60 
MSKAVGDLGL VGLAVMGQNL ILNAADHGFT VVAYNRTQSK VDRFLANEAK GKSIIGATSI 

        70         80         90        100        110        120 
EDLVAKLKKP RKIMLLIKAG APVDTLIKEL VPHLDKGDII IDGGNSHFPD TNRRYEELTK 

       130        140        150        160        170        180 
QGILFVGSGV SGGEDGARFG PSLMPGGSAE AWPHIKNIFQ SIAAKSNGEP CCEWVGPAGS 

       190        200        210        220        230        240 
GHYVKMVHNG IEYGDMQLIC EAYDIMKRIG RFTDKEISEV FDKWNTGVLD SFLIEITRDI 

       250        260        270        280        290        300 
LKFDDVDGKP LVEKIMDTAG QKGTGKWTAI NALDLGMPVT LIGEAVFARC LSAIKDERKR 

       310        320        330        340        350        360 
ASKLLAGPTV PKDAIHDREQ FVYDLEQALY ASKIISYAQG FMLIREAARS YGWKLNNPAI 

       370        380        390        400        410        420 
ALMWRGGCII RSVFLAEITK AYRDDPDLEN LLFNEFFASA VTKAQSGWRR TIALAATYGI 

       430        440        450        460        470        480 
PTPAFSTALA FYDGYRSERL PANLLQAQRD YFGAHTFRIL PECASAHLPV DKDIHINWTG 

       490 
HGGNISSSTY QA 

« Hide

References

« Hide 'large scale' references
[1]"Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene."
Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L.
Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X99228 Genomic DNA. Translation: CAA67612.1.
Z73041 Genomic DNA. Translation: CAA97285.1.
AY692811 Genomic DNA. Translation: AAT92830.1.
BK006941 Genomic DNA. Translation: DAA08347.1.
PIRS64588.
RefSeqNP_011772.3. NM_001181385.3.

3D structure databases

ProteinModelPortalP53319.
SMRP53319. Positions 6-479.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33508. 30 interactions.
DIPDIP-6605N.
IntActP53319. 1 interaction.
MINTMINT-702969.
STRING4932.YGR256W.

Proteomic databases

PaxDbP53319.
PeptideAtlasP53319.
PRIDEP53319.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGR256W; YGR256W; YGR256W.
GeneID853172.
KEGGsce:YGR256W.

Organism-specific databases

CYGDYGR256w.
SGDS000003488. GND2.

Phylogenomic databases

eggNOGCOG0362.
GeneTreeENSGT00390000009023.
HOGENOMHOG000255147.
KOK00033.
OMAPECASAH.
OrthoDBEOG7F252Z.

Enzyme and pathway databases

BioCycYEAST:YGR256W-MONOMER.
UniPathwayUPA00115; UER00410.

Gene expression databases

GenevestigatorP53319.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFPIRSF000109. 6PGD. 1 hit.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR00873. gnd. 1 hit.
PROSITEPS00461. 6PGD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973296.
PROP53319.

Entry information

Entry name6PGD2_YEAST
AccessionPrimary (citable) accession number: P53319
Secondary accession number(s): D6VV36
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways