P53318 (COQ6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquinone biosynthesis monooxygenase COQ6
EC=1.14.13.-

Gene names

Name:	COQ6
Ordered Locus Names:	YGR255C
ORF Names:	G9165

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	FAD Potential.
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.1.
Miscellaneous	Present with 2940 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UbiH/COQ6 family.
Sequence caution	The sequence CAA56705.1 differs from that shown. Reason: Frameshift at positions 212 and 434.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	FAD Flavoprotein
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquinone biosynthetic process Inferred from mutant phenotype PubMed 21944752PubMed 9266513. Source: SGD
Cellular_component	mitochondrial inner membrane Inferred from direct assay Ref.1. Source: SGD
Molecular_function	N,N-dimethylaniline monooxygenase activity Inferred from sequence alignment Ref.1. Source: SGD flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-4943,EBI-4943

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Ubiquinone biosynthesis monooxygenase COQ6		PRO_0000207581
Experimental info
	Sequence conflict	60	1	V → I in CAA56704. Ref.5
	Sequence conflict	68	1	K → R in CAA56704. Ref.5
	Sequence conflict	313	1	K → E in CAA56705. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P53318 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B6D4286545CCE695	FASTA	479	53,527
	10 20 30 40 50 60 MFFSKVMLTR RILVRGLATA KSSAPKLTDV LIVGGGPAGL TLAASIKNSP QLKDLKTTLV 70 80 90 100 110 120 DMVDLKDKLS DFYNSPPDYF TNRIVSVTPR SIHFLENNAG ATLMHDRIQS YDGLYVTDGC 130 140 150 160 170 180 SKATLDLARD SMLCMIEIIN IQASLYNRIS QYDSKKDSID IIDNTKVVNI KHSDPNDPLS 190 200 210 220 230 240 WPLVTLSNGE VYKTRLLVGA DGFNSPTRRF SQIPSRGWMY NAYGVVASMK LEYPPFKLRG 250 260 270 280 290 300 WQRFLPTGPI AHLPMPENNA TLVWSSSERL SRLLLSLPPE SFTALINAAF VLEDADMNYY 310 320 330 340 350 360 YRTLEDGSMD TDKLIEDIKF RTEEIYATLK DESDIDEIYP PRVVSIIDKT RARFPLKLTH 370 380 390 400 410 420 ADRYCTDRVA LVGDAAHTTH PLAGQGLNMG QTDVHGLVYA LEKAMERGLD IGSSLSLEPF 430 440 450 460 470 WAERYPSNNV LLGMADKLFK LYHTNFPPVV ALRTFGLNLT NKIGPVKNMI IDTLGGNEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin-dependent monooxygenase required for coenzyme Q biosynthesis." Gin P., Hsu A.Y., Rothman S.C., Jonassen T., Lee P.T., Tzagoloff A., Clarke C.F. J. Biol. Chem. 278:25308-25316(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.
[2]	"Sequence analysis of a 10.5 kb DNA fragment from the yeast chromosome VII reveals the presence of three new open reading frames and of a tRNAThr gene." Mazzoni C., Ruzzi M., Rinaldi T., Solinas F., Montebove F., Frontali L. Yeast 13:369-372(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	Miosga T. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF003698 Genomic DNA. Translation: AAB61341.1. X99228 Genomic DNA. Translation: CAA67617.1. Z73040 Genomic DNA. Translation: CAA97284.1. X80690 Genomic DNA. Translation: CAA56704.1. Frameshift. X80690 Genomic DNA. Translation: CAA56705.1. Frameshift. BK006941 Genomic DNA. Translation: DAA08346.1.
PIR	S64587.
RefSeq	NP_011771.1. NM_001181384.1.
3D structure databases
ProteinModelPortal	P53318.
SMR	P53318. Positions 25-67, 184-438.
ModBase	Search...
Protein-protein interaction databases
IntAct	P53318. 6 interactions.
MINT	MINT-619446.
Proteomic databases
PaxDb	P53318.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR255C; YGR255C; YGR255C.
GeneID	853170.
KEGG	sce:YGR255C.
Organism-specific databases
CYGD	YGR255c.
SGD	S000003487. COQ6.
Phylogenomic databases
eggNOG	COG0654.
GeneTree	ENSGT00390000015152.
HOGENOM	HOG000255772.
KO	K06126.
OMA	MMQSVDI.
OrthoDB	EOG496316.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16019. YEAST:YGR255C-MONOMER.
UniPathway	UPA00232.
Gene expression databases
Genevestigator	P53318.
GermOnline	YGR255C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002938. mOase_FAD-bd. IPR003042. Rng_hydrolase-like. IPR018168. Ubi_Hdrlase_CS. IPR010971. Ubi_Hdrxlases. IPR000689. UbQ_biosynth_mOase. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
TIGRFAMs	TIGR01989. COQ6. 1 hit. TIGR01988. Ubi-OHases. 1 hit.
PROSITE	PS01304. UBIH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973291.

Entry information

Entry name

COQ6_YEAST

Accession

Primary (citable) accession number: P53318
Secondary accession number(s): D6VV35, Q05674, Q05675

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program