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Protein

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Gene

CPD1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the metabolism of ADP-ribose 1',2'-cyclic phosphate which is produced as a consequence of tRNA splicing.2 Publications

Miscellaneous

Present with 1520 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.

Kineticsi

  1. KM=4.97 mM for adenosine 1',2'-cyclic phosphate2 Publications
  2. KM=14.29 mM for guanosine 1',2'-cyclic phosphate2 Publications
  3. KM=12.18 mM for cytidine 1',2'-cyclic phosphate2 Publications
  4. KM=8.91 mM for uridine 1',2'-cyclic phosphate2 Publications
  5. KM=0.37 mM for ADP-ribose 1',2'-cyclic phosphate2 Publications
  6. KM=0.33 mM for ribose 1,3-cyclic phosphate2 Publications
  1. Vmax=353 µmol/min/mg enzyme toward adenosine 1',2'-cyclic phosphate2 Publications
  2. Vmax=390 µmol/min/mg enzyme toward guanosine 1',2'-cyclic phosphate2 Publications
  3. Vmax=598 µmol/min/mg enzyme toward cytidine 1',2'-cyclic phosphate2 Publications
  4. Vmax=296 µmol/min/mg enzyme toward uridine 1',2'-cyclic phosphate2 Publications
  5. Vmax=61 µmol/min/mg enzyme toward ADP-ribose 1',2'-cyclic phosphate2 Publications
  6. Vmax=0.02 µmol/min/mg enzyme toward ribose 1,3-cyclic phosphate2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei39Proton donor/acceptorBy similarity1
Binding sitei41SubstrateBy similarity1
Active sitei150Proton donor/acceptorBy similarity1
Binding sitei152SubstrateBy similarity1
Binding sitei155SubstrateBy similarity1

GO - Molecular functioni

  • 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity Source: SGD

GO - Biological processi

  • cyclic nucleotide metabolic process Source: SGD

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciMetaCyc:YGR247W-MONOMER.
YEAST:YGR247W-MONOMER.
SABIO-RKiP53314.

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 3'-phosphodiesterase (EC:3.1.4.37)
Short name:
CPDase
Gene namesi
Name:CPD1
Ordered Locus Names:YGR247W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR247W.
SGDiS000003479. CPD1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39H → A: Abolishes cyclic nucleotide phosphodiesterase activity. 1 Publication1
Mutagenesisi41T → A: Reduces strongly cyclic nucleotide phosphodiesterase activity. 1 Publication1
Mutagenesisi150H → A: Abolishes cyclic nucleotide phosphodiesterase activity. 1 Publication1
Mutagenesisi152S → A: Reduces strongly cyclic nucleotide phosphodiesterase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002028591 – 2392',3'-cyclic-nucleotide 3'-phosphodiesteraseAdd BLAST239

Proteomic databases

MaxQBiP53314.
PRIDEiP53314.
TopDownProteomicsiP53314.

Interactioni

Protein-protein interaction databases

BioGridi33498. 49 interactors.
DIPiDIP-5558N.
IntActiP53314. 1 interactor.
MINTiMINT-477473.
STRINGi4932.YGR247W.

Structurei

3D structure databases

ProteinModelPortaliP53314.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000111920.
KOiK15435.
OMAiFEPHITI.
OrthoDBiEOG092C4YE1.

Family and domain databases

Gene3Di3.90.1140.10. 1 hit.
InterProiView protein in InterPro
IPR009097. cNuc_Pdiesterase.
IPR012386. Cyclic-nucl_3Pdiesterase.
PANTHERiPTHR28141. PTHR28141. 1 hit.
PfamiView protein in Pfam
PF07823. CPDase. 1 hit.
SUPFAMiSSF55144. SSF55144. 2 hits.

Sequencei

Sequence statusi: Complete.

P53314-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIALWYCPP QGSVAYETLQ MLIFSFQTLF PDSPVFEPHV TVTSHLVCNS
60 70 80 90 100
KDDVNKILTS CVAAIQSIRS HQTAKKGRKG QVSHAVAAPL VSFNGCSVGK
110 120 130 140 150
QYFKKIVLEC NKNKILYGVA QVMREMYVEI DPETRSSRAA TWVHEEFHPH
160 170 180 190 200
VSLLYSDIHP VSQASLRVVQ QRIEDALDVQ LVPREKRKGS GNADGSNEVQ
210 220 230
MRWDFDVSSS LSWNIPGTFK VVNCVGPVQE WEVLGRVDV
Length:239
Mass (Da):26,732
Last modified:October 1, 1996 - v1
Checksum:iDE38A74FAD0E3A55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07703 Genomic DNA. Translation: CAA68969.1.
Z73032 Genomic DNA. Translation: CAA97276.1.
AY558381 Genomic DNA. Translation: AAS56707.1.
BK006941 Genomic DNA. Translation: DAA08338.1.
PIRiS64573.
RefSeqiNP_011763.1. NM_001181376.1.

Genome annotation databases

EnsemblFungiiYGR247W; YGR247W; YGR247W.
GeneIDi853162.
KEGGisce:YGR247W.

Similar proteinsi

Entry informationi

Entry nameiCPD1_YEAST
AccessioniPrimary (citable) accession number: P53314
Secondary accession number(s): D6VV27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names