P53312 (SUCB_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
EC=6.2.1.5

Alternative name(s):
Succinyl-CoA synthetase beta chain
Short name=SCS-beta

Gene names

Name:	LSC2
Ordered Locus Names:	YGR244C
ORF Names:	G8625

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + succinate + CoA = ADP + phosphate + succinyl-CoA. Ref.4
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.
Subunit structure	Heterodimer of an alpha and a beta subunit By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the succinate/malate CoA ligase beta subunit family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	succinyl-CoA metabolic process Inferred from direct assay Ref.4. Source: SGD tricarboxylic acid cycle Traceable author statement. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.4. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW succinate-CoA ligase (ADP-forming) activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 427		Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial		PRO_0000033365
Regions
	Domain	39 – 267	229	ATP-grasp
Amino acid modifications
	Modified residue	102	1	Phosphoserine Ref.5 Ref.6
	Modified residue	207	1	Phosphoserine Ref.6
	Modified residue	263	1	Phosphoserine Ref.6
	Modified residue	276	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P53312 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 12DF242D4D148648	FASTA	427	46,901
	10 20 30 40 50 60 MYSRKSLSLI SKCGQLSRLN AQAALQARRH LSIHEYRSAQ LLREYGIGTP EGFPAFTPEE 70 80 90 100 110 120 AFEAAKKLNT NKLVIKAQAL TGGRGKGHFD TGYKSGVHMI ESPQQAEDVA KEMLNHNLIT 130 140 150 160 170 180 KQTGIAGKPV SAVYIVKRVD TKHEAYLSIL MDRQTKKPMI IASSQGGMNI EEVAERTPDA 190 200 210 220 230 240 IKKFSIETSK GLSPQMAKDV AKSLGFSPDA QDEAAKAVSN LYKIFMERDA TQVEINPLSE 250 260 270 280 290 300 IEHDPTHKIM CTDAKFGFDD NASFRQEKIY SWRDLSQEDP DEVKAKKYDL NFVKLKGNIG 310 320 330 340 350 360 CLVNGAGLAM ATMDVIKLNG GDPANFLDCG GGATPETIKQ GFELILSNKN VDAIFVNIFG 370 380 390 400 410 420 GIVRCDYVAL GLVEAARELE VRVPIVARLQ GTKVEEGRDI INKSGVKIYS FDELDPAAKK VVELTQN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII." Guerreiro P., Azevedo D., Barreiros T., Rodrigues-Pousada C. Yeast 13:275-280(1997) [PubMed: 9090057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Genes of succinyl-CoA ligase from Saccharomyces cerevisiae." Przybyla-Zawislak B., Dennis R.A., Zakharkin S.O., McCammon M.T. Eur. J. Biochem. 258:736-743(1998) [PubMed: 9874242] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY.
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102; SER-207; SER-263 AND SER-276, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z73029 Genomic DNA. Translation: CAA97273.1. BK006941 Genomic DNA. Translation: DAA08335.1.
PIR	S64570.
RefSeq	NP_011760.1. NM_001181373.1.
3D structure databases
ProteinModelPortal	P53312.
SMR	P53312. Positions 31-427.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4003N.
IntAct	P53312. 2 interactions.
MINT	MINT-520241.
STRING	P53312.
2D gel databases
UCD-2DPAGE	P53312.
Proteomic databases
PeptideAtlas	P53312.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR244C; YGR244C; YGR244C.
GeneID	853159.
KEGG	sce:YGR244C.
NMPDR	fig|4932.3.peg.2889.
Organism-specific databases
CYGD	YGR244c.
SGD	S000003476. LSC2.
Phylogenomic databases
eggNOG	fuNOG07621.
GeneTree	EFGT00050000003524.
HOGENOM	HBG315432.
OMA	KKELYVS.
OrthoDB	EOG42Z7ZK.
Gene expression databases
ArrayExpress	P53312.
Genevestigator	P53312.
GermOnline	YGR244C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013650. ATP-grasp_succ-CoA_synth-type. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR005811. CoA_ligase. IPR017866. Succ-CoA_synthase_bsu_CS. IPR005809. Succ_CoA_synthase_bsu. IPR016102. Succinyl-CoA_synth-like. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
KO	K01900.
PANTHER	PTHR11815. CoA_lig_beta. 1 hit.
Pfam	PF08442. ATP-grasp_2. 1 hit. PF00549. Ligase_CoA. 1 hit. [Graphical view]
PIRSF	PIRSF001554. SucCS_beta. 1 hit.
SUPFAM	SSF52210. CoA_ligase. 1 hit.
TIGRFAMs	TIGR01016. SucCoAbeta. 1 hit.
PROSITE	PS50975. ATP_GRASP. False negative. PS01217. SUCCINYL_COA_LIG_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973258.

Entry information

Entry name

SUCB_YEAST

Accession

Primary (citable) accession number: P53312
Secondary accession number(s): D6VV24

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program